1.Association between insulin resistance and coronary artery disease
Chinese Journal of General Practitioners 2005;0(07):-
Objective To study the effect of insulin resistance on the development of coronary artery disease ( CAD). Methods In total, 204 nondiabetic inpatients diagnosed by elective coronary angiography were selected in the study and divided into CAD and control groups. Relationship between insulin resistance assessed by homeostasis model ( HOMA-IR) and fasting serum level of insulin and occurrence of CAD and the extent of stenosis of coronary arteries was analyzed. Results IR and serum level of insulin were significantly higher in CAD group than those in control group ( P
2.Comparison of tensionfree herniorrhaphy with conventional herniorrhaphy for inguinal hernia
Jiye LI ; Qun DENG ; Xinkui ZHANG ; Tao LI ; Shibin WANG ; Huabo JIAO ; Zhanliang LI
Chinese Journal of General Surgery 2001;0(08):-
ObjectiveTo evaluate the value of tensionfree herniorrhaphy for inguinal hernia. Methods(1)Comparison was made in operating time, early postoperative response, hospitalization time, postoperative complications and recurrence rate between the two herniorrhaphy groups.In tensionfree herniorrhaphy group( n=137),hernias were repaired with polypropylene mesh plug or mesh patch.(2)In conventional herniorrhaphy group (n=98 ) ,hernias were repaired with Bassini operation. Mean Follow up time was 22 month in the 2 groups. Results The operating time, analgesic use, early response and hospitaliz ation time in tensionfree herniorrhaphy group were significantly lower than those in conventional herniorrhaphy group(P
3.Relationship between ZNF139 and multidrug resistance(MDR) related genes in SGC7901 and SGC7901/ADR cell lines
Yong LI ; Qun ZHAO ; Bibo TAN ; Liqiao FAN ; Qingwei LIU ; Zhikai JIAO ; Xuefeng ZHAO ; Yingjie HAO
China Oncology 2013;(7):493-498
Background and purpose:It was reported that zinc ifnger protein 139 (ZNF139) was expressed aberrantly in gastric cancer. But the relationship between ZNF139 and multidrug resistance (MDR) of gastric cancer is still not clear. The purpose of this research was to investigate the expressions and signiifcance of ZNF139, MRP-1, MDR1/P-gp, GST-π in human gastric carcinoma cell lines SGC7901 and SGC7901/ADR. Methods: The expressions of ZNF139, MRP-1, MDR1/P-gp, GST-πwere determined with RT-PCR and Western blot in SGC7901 and SGC7901/ADR cell lines. Then siRNA recombinant plasmid of targeting ZNF139 gene was constructed and imported into gastric cancer cell line SGC7901/ADR, and the expressions of MRP-1, MDR1/P-gp, GST-πwere tested simultaneously. Results:The expressions of ZNF139, MRP-1, MDR1/P-gp, GST-πwere higher in SGC7901/ADR than in SGC7901(P<0.05). ZNF139 was inhibited obviously after siRNA-ZNF139 was transfected into SGC7901/ADR, and expression of MRP-1, MDR1/P-gp, GST-πdecreased(P<0.05). Conclusion:ZNF139 may be invovled in multidrug resistance (MDR) of gastric cancer by up-regulating MRP-1, MDR1/P-gp and GST-π.
4.The Distribution and Drug Resistance of the Common Clinical Gram-Negative Bacillus
Jin-Gui CAO ; Xiao-Feng HE ; Di WU ; Li-Qun JIAO ; Hu ZHANG ;
Microbiology 1992;0(02):-
To determine the distribution and drug resistance tendency of Gram-negative bacillus so as to provide a reasonable selection for antibiotic in clinical treatment.The clinical isolates bacillus were mainly Gram-Negative bacillus during three years.The detection out ratio for Gram-Negative bacillus had decreased gradually but the ratio for non-fermental gram-negative bacillus had increased.The combinations of antibiotic and enzyme inhibitor has more antibacterial activity to non-fermental gram-negative bacillus than antibiotic.The detection out ratio for ESBLs had gradually increased.
5.Surgical treatment for severe distortion of the internal carotid artery
Li-Yong ZHANG ; Wei-Dong LIU ; Li-Xin DU ; Ji-Yue WANG ; Li-Qun JIAO
Chinese Journal of Neuromedicine 2013;12(2):166-170
Objective To evaluate and summarize the possibility and experience of reconstructive vascular operation for V-shaped kinking of internal carotid artery.Methods Reconstructive vascular operation was performed in 11 patients with symptomatic V-shaped kinking of internal carotid artery,admitted to our hospitals from July 2008 to May 2010; 7 of them underwent eversion endarterectomy simultaneously.Results One patient with severe distortion appeared mild agitation,insomnia and headache 3-5 d after the operation,but 1 week later,the system disappeared completely.CTA right after the operation showed that the distortion vascular was straight with good vascular patency.All patients rechecked MR diffusion weighted imaging 1 week after the operation,showing that 2 patients had fresh lacunar infarction in the operation side.Carotid ultrasonography showed no restenosis recurrence,distortion or hemodynamic disorders 3 and 6 months after the operation.1patient rechecked CT brain perfusion imaging 6 months after the operation,showing obviously improved perfusion.Follow up for 6 months reviewed no other new infarction lesions,no obvious nervous signs or symptoms appeared; no one reappeared TIA or had operative complications.Obvious improvement of spirit and reaction was noted in a patient with slow response.Conclusion Reconstructive vascular operation is an effective surgical approach in kinking and coiling of the internal carotid artery.
6.The cytotoxic effect and injury mechanism of deoxynivalenol on articular chondrocytes in human embryo.
Hai-Feng HOU ; Jin-Ping LI ; Guo-Yong DING ; Wen-Jing YE ; Peng JIAO ; Qun-Wei LI
Chinese Journal of Preventive Medicine 2011;45(7):629-632
OBJECTIVEThis study was to explore the cytotoxic effect and the related injury mechanism of deoxynivalenol (DON) on articular chondrocytes in human embryo.
METHODSArticular cartilage cells were isolated from knees of human embryo and cultured in DMEM/F12 medium. The cells of the 4th generation were divided into five groups and incubated with varying concentrations of DON as the followings: control group and group with DON of 0.1, 0.2, 0.4, 1.0 µg/ml. The effects of DON were observed 72 hours after incubation. Cell apoptosis was assayed by flow cytometry (FCM) with Annexin V-FITC/PI staining; MMP-13 and PGE2 were detected by ELISA kits; NO was measured by Griess assay with spectrophotometer. Inducible nitric oxide synthase (iNOS) and collagen II in cells were detected by FCM. The expression levels of iNOS, mRNA and collagen II mRNA were measured with RT-PCR.
RESULTSThe rates of cell apoptosis in DON groups were 6.78% - 19.05%, which were significantly higher than that in control (1.20%, F = 174.761, P < 0.05). The levels of NO in DON groups were 20.8 - 40.7 µmol/L, which were significantly higher than that in control (10.2 µmol/L, F = 91.966, P < 0.05). The levels of MMP-13 in DON groups were 0.25 - 0.56 µmol/L, which were significantly higher than that in control (0 µmol/L, F = 78.420, P < 0.05). The levels of PGE2 in DON groups were 3.2-20.6 µmol/L, which were significantly higher than that in control (11.6 µmol/L, F = 276.453, P < 0.05). The proportions of cells with positive iNOS in DON groups were 14.8% - 56.8% which were significantly higher than that in controls (7.1%, F = 214.614, P < 0.05). The proportions of cells with positive collagen II in groups with DON of 0.4 µg/ml and 1.0 µg/ml were 56.7% and 52.7%, which were significantly lower than that in control (62.2%, F = 5.134, P < 0.05). The relative absorbance values of iNOS mRNA in DON groups were 1.07 - 1.33, which were significantly higher than that in control (0.62, F = 8.358, P < 0.05). The levels of collagen II mRNA in groups with DON of 0.4 µg/ml and 1.0 µg/ml were 0.83 and 0.82, which were significantly lower than that in control (1.14, F = 7.887, P < 0.05).
CONCLUSIONDON could promote anabolism of NO in articular cartilage cells by which up-regulated the expression of PGE2 and MMP-13, which both promoted resolution of articular cartilage matrix such as collagen II. DON induced apoptosis in articular cartilage cells.
Cartilage, Articular ; cytology ; embryology ; Cells, Cultured ; Chondrocytes ; drug effects ; metabolism ; Dinoprostone ; metabolism ; Humans ; Matrix Metalloproteinase 13 ; metabolism ; Nitric Oxide ; biosynthesis ; Trichothecenes ; toxicity
7.Determination of serum acute phase reaction protein in patients with severe acute respiratory syndrome.
Wei-jiao LIAO ; Yi-min LI ; Tao CHEN ; Wei-qun HE ; Yong-ping LIN ; Ning LI
Chinese Journal of Preventive Medicine 2004;38(2):92-93
OBJECTIVETo study the diagnostic value of determinations of serum acute phase reaction protein, such as complement 3 (C3), complement 4 (C4), prealbumin (PA) and C-reactive protein (CRP), etc., in patients with severe acute respiratory syndrome (SARS).
METHODSSerum levels of C3, C4, PA and CRP were determined by turbidimetry in 54 cases of SARS, 20 of other pneumonia and 30 normal persons.
RESULTSSerum concentrations of C3, C4, CRP and PA were (1.18 +/- 0.42) g/L, (1.15 +/- 0.56) g/L, (10.52 +/- 8.77) mg/L and (107 +/- 54) mg/L in SARS patients, (1.30 +/- 0.46) g/L, (0.57 +/- 0.31) g/L, (0.88 +/- 0.43) mg/L and (291 +/- 76) mg/L in patients with other pneumonia, and (1.11 +/- 0.56) g/L, (0.38 +/- 0.26) g/L, (0.42 +/- 0.26) mg/L and (376 +/- 74) mg/L in normal persons, respectively. Serum level of PA was significantly lower and levels of C4 and CRP significantly in patients with SARS higher than those in patients with other pneumonia and normal persons (P < 0.01). There was no significant difference in serum level of C3 between the three groups (P > 0.05).
CONCLUSIONDetermination of serum level of C4, CRP and PA in suspected patients is beneficial to early differential diagnosis for SARS.
Acute-Phase Proteins ; analysis ; Acute-Phase Reaction ; blood ; Adult ; Aged ; C-Reactive Protein ; analysis ; Complement C3 ; analysis ; Complement C4 ; analysis ; Female ; Humans ; Male ; Middle Aged ; Prealbumin ; analysis ; Severe Acute Respiratory Syndrome ; blood
8.Mutation analysis of the HBV reverse transcriptase in nucleos(t)ide-treated patients with chronic HBV infection.
Xiu-Juan JIAO ; Xun PENG ; Xiu-Min JIAO ; Jin-Sheng WANG ; Xie-Wen SUN ; Pei-Li ZHAO ; Shou-Yun WANG ; Jia-Qun LIU ; Tong LI ; Jing-Xian YANG
Chinese Journal of Experimental and Clinical Virology 2012;26(6):453-455
OBJECTIVETo characterize genotypic resistance within HBV RT region in chronic hepatitis B (CHB) patients with nucleos(t)ide analogue (NA) treatment.
METHODSSerum samples of 229 CHB patients with NA treatment were obtained. Full-length HBV RT sequences were amplified, sequenced and analyzed, on the following NA resistant (NAr) mutations belonging to different NAr pathways.
RESULTSAmong 229 HBV isolates, 14.41% (33/229) and 85.59% (196/229) were genotype B and C, respectively; and the patients with HBV genotype C may be more susceptible to develope resistant mutations than patients with HBV genotype B(chi2 = 2.95, P < 0.05). NAr mutations were detected in 63 CHB patients. Mutations were not found at rtI169, rtT184, rtA194 or rtS202. RtM204 mutations were detected at the highest frequency among 63 mutants (40/63, 63.49%) and found to display 11 combination mutation patterns, in which rtM204I were associated with rtL80I/V and rtL180M, and rtM204V were associated with rtL1l80M, respectively. Conclusions There are complicated mutation patterns in the HBV RT region for chronic hepatitis B (CHB) patients with nucleos(t)ide analogue (NA) treatment. RtM204V/I mutation was the highest.
Adolescent ; Adult ; Aged ; Antiviral Agents ; therapeutic use ; Female ; Hepatitis B virus ; drug effects ; enzymology ; genetics ; Hepatitis B, Chronic ; drug therapy ; virology ; Humans ; Male ; Middle Aged ; Mutation ; drug effects ; Nucleosides ; therapeutic use ; Nucleotides ; therapeutic use ; RNA-Directed DNA Polymerase ; genetics ; metabolism ; Viral Proteins ; genetics ; metabolism ; Young Adult
9.Optimization of atrioventricular delay by surface electrocardiography during dual chamber pacing.
Li-qun WU ; Gang GU ; Min CAO ; Yong-chu SHEN ; Kan SU ; Wei-feng SHEN
Chinese Medical Journal 2006;119(6):454-457
BACKGROUNDNominal atrioventricular (AV) interval in dual chamber pacemaker (DDD) is not the best AV delay in the majority of patients with atrioventricular block. To find a simple method for optimizing AV delay adjustment, we assessed surface electrocardiography (ECG) for optimizing AV delay during dual chamber pacing.
METHODSDDD pacemakers were implanted in 46 patients with complete, or almost complete, AV block. Optimal AV delay was achieved by programming an additional delay of 100 ms, to the width of intrinsic P wave or to the interval between pacing spike to the end of P wave on surface ECG. Left ventricular (LV) end diastolic and end systolic volumes, ejection fraction and diastolic parameters were measured by Doppler echocardiography during both nominal and optimal AV delay pacing.
RESULTSCompared to nominal AV delay setting, LV end diastolic volume increased [to (53.2 +/- 11.3) ml from (50.2 +/- 10.2) ml, P < 0.05], end systolic volume decreased [to (26.1 +/- 9.0) ml from (27.9 +/- 8.2) ml, P < 0.05] during adjusted AV delay pacing, resulting in an increase in LV ejection fraction [to (68.2 +/- 5.3)% from (64.5 +/- 4.3)%, P < 0.05]. LV diastolic filling and isovolumic relaxation time were not significantly changed.
CONCLUSIONOptimization of AV delay by surface ECG is a simple method to improve LV systolic function during dual chamber pacing.
Adult ; Aged ; Aged, 80 and over ; Atrioventricular Node ; physiopathology ; Cardiac Pacing, Artificial ; methods ; Electrocardiography ; methods ; Female ; Heart Block ; physiopathology ; therapy ; Humans ; Male ; Middle Aged ; Time Factors
10.The effects of aloesin and arbutin on cultured melanocytes in a synergetic method.
Zhuang-qun YANG ; Zheng-hui WANG ; Jun-bo TU ; Peng LI ; Xiao-yi HU
Chinese Journal of Plastic Surgery 2004;20(5):369-371
OBJECTIVETo study the effects of aloesin and arbutin on normal cultured human melanocytes in synergetic method.
METHODSBuilding up the system of cultured human melanocytes. The cultured melanocytes in vitro were treated with the mixture of aloesin and arbutin. The cell viability and tyrosinase activity was measured by MTT assay, utilization of L-Dopa as the substrate respectively; melanin content was measured by image analysis system. Furthermore, the effects of the mixture on melanocytes were compared with that of aloesin and arbutin.
RESULTSThe mixture of aloesin and arbutin showed an inhibition on tyrosinase activity of human melanocytes and reduced significantly melanin content. Between the mixture and the single use of aloesin or arbutin, there is significant difference (P < 0.05). On the other hand, the mixture has little influence on melanocytes viability and there is negative significance.
CONCLUSIONThe mixture of aloesin and arbutin can significantly inhibit the tyrosinase activity and melanogenesis of cultured human melanocytes. It showed the effects of aloesin and arbutin in a synergistic manner. It is worth to give farther study later.
Arbutin ; pharmacology ; Cells, Cultured ; Chromones ; pharmacology ; Dose-Response Relationship, Drug ; Drug Synergism ; Glucosides ; pharmacology ; Humans ; Melanocytes ; cytology ; drug effects ; metabolism ; Monophenol Monooxygenase ; drug effects ; metabolism