1.Surface Enhanced Raman Spectroscopy Sensor Based on Magnetic Beads-induced Nanoparticles Aggregation for Detection of Bacterial Deoxyribonucleic Acid
Qun MA ; Yanle LI ; Nianchun GONG ; Xi JIANG ; Shuangyan HUAN
Chinese Journal of Analytical Chemistry 2015;(11):1676-1681
A novel method based on the separation and enrichment effect of magnetic beads and the fully complementary hybridization of two DNA strands was developed for highly sensitive detection of bacterial DNA using a surface-enhanced Raman spectroscopy (SERS) with 5,5’-dithio-bis(2-nitrobenzoic acid) (DTNB)-modified gold nanoparticles as reporter probes. Capture probe was immobilized onto the surface of streptavidin-enwrapped magnetic beads ( SA-MB ) through high affinity between biotin and avidin, by which the target bacterial DNA sequences that connected with the reported probe decorated AuNPs with DTNB and SH-DNA ( AuNPs@DTNB@DNA) were captured and loaded onto the magnetic beads by the hybridization reaction with the capture probe. Compared with previous methods, this design shortened the distance between particles by the ways that the magnetic beads tempted to nanoparticles aggregation, and produced the plasma resonance coupling effect, which increased the SERS signal significantly. The results showed that, under the optimized conditions and in the concentration range from 5 pmol/L to 5 nmol/L, the method performed a good linear relationship between Raman intensity and DNA concentration. The limit of detection ( LOD) of bacterial DNA was estimated to be 5 pmol/L. The method is simple and low cost, and can be used in the sensitive and selective detection of bacterial DNA.
2.Small cell carcinoma of the gastric cardia: a histopathological analysis.
Li-qun GONG ; Ya-zhou AO ; Chuan-gui ZHANG
Chinese Journal of Oncology 2006;28(3):216-217
Adult
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Aged
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Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Carcinoma, Small Cell
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metabolism
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pathology
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therapy
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Cardia
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Chemotherapy, Adjuvant
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Chromogranin A
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metabolism
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Female
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Follow-Up Studies
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Gastrectomy
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methods
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Humans
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Male
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Middle Aged
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Phosphopyruvate Hydratase
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metabolism
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Stomach Neoplasms
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metabolism
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pathology
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therapy
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Survival Rate
3.WBC Increasing Medicine Usage Analysis in Cancer Patients in a Hospital
Na YANG ; Naping TANG ; Jianping GONG ; Qun LI ; Jian XIAO
China Pharmacist 2016;19(5):925-927
Objective:To investigate the application of leukogenic medicine in cancer patients and standardize the use of WBC increasing medicine in a hospital. Methods:A retrospective analysis of 579 patient records from January 2014 to December in a hospital diagnosed as malignant tumor and issued WBC increasing medicine . The kinds of drugs,timing of administration, dosage,frequency of administration,the use of medication,drug combination and the choice of solvent were analysed by collecting the patients’ essential information, chemotherapy, myelosuppression etc. Results: The l white blood drugs mainly oral preparations;There were 414 rational drug use medication orders,accounting for 71. 50 percent;irrational drug use medical record 165,accounting for 28. 50% . The dosage and duration of treatment in line with requirements,and unreasonable cases mainly suitable varieties and timing of administration based,42. 42% and 27. 88% ,respectively;followed by repeated dosing and combination therapy is not suitable,13. 33% 1and 2. 73,respectively;a lower incidence of solvent choice unsuitable,is 3. 64% . Conclusion:The use of WBC increasing medicine need to be standardized in cancer patients in the hospital. Clinicians need to strengthen studying the knowledge of the application of WBC increasing medicine and use WBC increasing medicine rationally.
4.Novel germline mutations in FLCN gene identified in two Chinese patients with Birt–Hogg–Dubé syndrome
Li TENG ; Ning XIANGHUI ; He QUN ; Gong KAN
Chinese Journal of Cancer 2017;36(2):99-102
Birt–Hogg–Dubé (BHD) syndrome, a hereditary renal cancer syndrome caused by mutations in the folliculin (FLCN) gene, is characterized by the presence of fibrofolliculomas, pulmonary cysts, spontaneous pneumothorax, and renal cell carcinoma (RCC). Few BHD syndrome cases have been reported in Asian countries, and cutaneous presenta-tions are relatively rare in Asian patients. Asian BHD patients may be misdiagnosed due to their atypical manifesta-tions. Here, we report two Chinese BHD patients with novel FLCN mutations (c.946-947delAG in exon 9 and c.770-772delCCT in exon 7). Both of them had RCC and spontaneous pneumothorax without fibrofolliculomas. In patients with RCC and pulmonary cysts but without cutaneous lesions, screening for mutations in the FLCN gene should be performed, especially for those with a family history of RCC or pulmonary cysts (pneumothorax).
5.Surgical treatment of acute biliary pancreatitis
Jun ZHOU ; Yixiong LI ; Huihuang TANG ; Xianwei WANG ; Xuejun GONG ; Guohuang HU ; Qun HE
Chinese Journal of General Surgery 1997;0(06):-
Objective To investigate the timing of operation and the selection of surgical procedure for acute biliary pancreatitis.Methods The clinical data of 247 cases of acute biliary pancreatitis were retrospectively analysed.Results Among 10 non-operation patients,4 died;12 patients underwent emergency operation,5 had postoperative complications and 2 patients died after operation.169 patients underwent delayed operation after effective conservative therapy and 1 patient had postoperative complication;56 patients underwent(selective) operation without any postoperative complication or death.Conclusions Early emergency operation should be performed for relief of biliary obstruction in acute biliary obstructive pancreatitis.The patients with acute non-obstructive biliary pancreatitis should undergo conservative therapy at first and then,after(pancreatitis) has been controlled,delayed operation or selective operation to treat the biliary pathologic condition should be performed.
6.Effect of probucol on plasma adiponectin levels of patients with type 2 diabetes mellitus
Qiangxiang LI ; Huiju ZHONG ; Feiyue ZHU ; Zhuo ZHANG ; Jinlian HE ; Hanren GONG ; Daojun SHEN ; Qun WU
Chinese Journal of Postgraduates of Medicine 2006;0(31):-
0.05). The blood glucose, glycosylated hemoglobin and oxidized low density lipoprotein degrade, insulin resistance were improved in probucol group after treatment, while the adiponectin level was increased(P
7.Determination of Paeonol in Yuxianling Granules by HPLC
Qun HE ; Pingan LIU ; Biqing ZHAO ; Wanzhong LI ; Wei GONG ; Xiao LIANG
China Pharmacy 2005;0(15):-
OBJECTIVE: To establish a HPLC method for the determination of Paeonol in Yuxianling granules. METHODS: The determination was performed on Phenomenex C18-ODS with mobile phase consisted of methanol-water (60∶40) at a flow rate of 1.0mL?min-1. The detection wavelength was set at 274nm. RESULTS: The linear range of Paeonol was 8.24~41.20?g?mL-1(r=0.999 7). The recovery was 99.50% (RSD=1.12%). CONCLUSION: The established method is simple, rapid, sensitive, accurate, stable and reproducible, and suitable for the determination of the content of paeonol in Yuxianling granules.
8.The surgical treatment and prognosis of bilateral sporadic renal tumors.
Ning ZHANG ; Kan GONG ; Li-qun ZHOU ; Yong YANG ; Yan-qun NA
Chinese Journal of Surgery 2007;45(2):121-123
OBJECTIVETo evaluate the surgical management and prognostic characteristics of bilateral sporadic renal tumors (BSRTs).
METHODSTwenty-three cases of BSRTs were identified and retrospectively reviewed. The presenting clinical findings, surgical treatment, and prognosis were evaluated.
RESULTSTwelve patients had synchronous tumors and 11 had asynchronous tumors. BSRTs comprised 1.8% of all RCC cases with a male-to-female ratio of 6.67:1 in the same term. The mean age of patients was 56 years. Seventeen patients were preformed bilateral operation (10 had synchronous and 7 had asynchronous tumors), 4 patients were preformed unilateral operation (1 had synchronous and 3 had asynchronous tumors), and 2 patients were not performed operation (asynchronous tumors). Twenty-one patients were followed up (11 had synchronous tumors and 10 had asynchronous tumors, mean 27 months). One and 5-years cancer specific survival rate of BSRTs were 84.6% and 33.5% respectively. The rate of renal failure of synchronous BSRTs (18%) was lower than that of asynchronous BSRTs (70%) after operation (P=0.030).
CONCLUSIONSPrognosis of BSRTs is poor. The key in the treatment of BSRTs is to detect and resect tumor earlier. Our results indicate that nephron sparing surgery should be performed in order to obtain optimal functional preservation of the renal remnant with complete local excision of tumor.
Adult ; Aged ; Carcinoma, Renal Cell ; pathology ; surgery ; Female ; Follow-Up Studies ; Humans ; Kaplan-Meier Estimate ; Kidney ; pathology ; surgery ; Kidney Neoplasms ; pathology ; surgery ; Male ; Middle Aged ; Nephrectomy ; methods ; Prognosis ; Retrospective Studies ; Treatment Outcome
9.Research in the replacement of heated humidifiers with heat moisture exchangers during mechanical ventilation
Xiaoling LV ; Qiujiang XIA ; Qun LUO ; Zongjun MU ; Yanan TAN ; Gaoyan JIANG ; Yan ZHOU ; Tingting GONG ; Yuan LI
Chinese Journal of Practical Nursing 2009;25(20):8-10
Objective To study the feasibility of using heat and moisture exchangers (HME)as an alternative to heated humidifiers (HH) in patients undergoing mechanical ventilation. Methods 266 pa-tients with mechanical ventilation admitted to our ICU over the recent 3 years were allocated to the experi-mental group (humidification with a heat and moisture exchanger) and the control group (with heated hu-midifier), and the effect of humidification, the reserved time of artificial airway, the time on mechanical yen-tilation, the time of stay in ICU, the ineidenee of ventilator-associated pneumonia (VAP) and the mor-tality rate were comparatively studied and analyzed. Results Significant differences were found between the experimental and the control group in effect of humidification, insufficiency of humidification or excessive hu-midification, airway spasm and time on mechanical ventilation and time of stay in ICU. The incidence of VAP in the control group was significantly higher than that in the experimental group. There were no significant dif-ference between the two groups in the reserved time of artificial airway and the mortality rate. There were no accident of humidification occurred in the experimental group while there were one case complicated with air-way burn and 11 eases complicated with choking with water in the control group. Conclusions We conclude that HH can be replaced by HME on mechanical ventilation while disease evolution and effect of humidification should be monitored closely and keep HME unobstructed.
10.Differential transcription of Bcl-2 and Bax through the cell cycle in prostate cancer cell line.
Xue-Song LI ; Kan GONG ; Guo-Feng SUN ; Li-Qun ZHOU ; Zhi-Song HE ; Dian-Qi XIN ; Yan-Qun NA ; Yun-Yan LIANG ; Dai-Shu WANG ; Ying-Lu GUO
Chinese Journal of Surgery 2008;46(10):768-771
OBJECTIVETo investigate the differential expression of apoptosis associated gene Bcl-2 and Bax through cell cycle and its possible clinical meaning.
METHODSThe prostate cancer cell line PC-3 was synchronized in M, G1, S and G2 phase using modified thymine deoxyriboside blockage and high pressure N2O technique. The efficiency of synchronization was detected by flow-cytometry. RT-PCR and Western blot methods were used to examine the expression of Bcl-2 and Bax in mRNA and protein level.
RESULTSThe synchronized rate of M, G1, S and G2 phase were 92.1%, 87.0%, 80.2% and 75.9% respectively. Bcl-2 was constitutively expressed through the cell cycle, but both the mRNA and protein expression level of Bcl-2 were very high in the G1 phase, dramatically decreased in M, S and G2 phase. The expression level of Bax had no change through the cell cycle.
CONCLUSIONSCell cycle could influence the expression level of Bcl-2 significantly but not Bax, these might have some clinical relevance.
Cell Cycle ; Cell Line, Tumor ; Gene Expression ; Humans ; Male ; Prostatic Neoplasms ; metabolism ; pathology ; Proto-Oncogene Proteins c-bcl-2 ; biosynthesis ; genetics ; RNA, Messenger ; genetics ; bcl-2-Associated X Protein ; biosynthesis ; genetics