Objective To acknowledge the effect of T-lymphocyte subset on lymph node micrometastasis in esophageal cancer patients by the study of the correlation between T-lymphocyte subset and micrometastasis.Methods CD3+、CD4+、CD8+and CD4+/CD8+ were examined in esophageal cancer patients under thoracotomy by flow cytometry,and the micrometastasis were examined by immunohistochemistry in lymph node that were diagnosed as negative for metastases by routine histopathological analysis.Two groups were divided by with or without micro-metastasis,T-lymphocyte subset were compared with two groups.Results CD3+,CD4+ and CD4+/CD8+were significantly lower in group with micrometastasis than in group without micrometastasis,CD8+ was significantly higher in group with micrometagtasis than in group without micrometastasis.Conclusion T-lymphocyte subset is closely associmed with micrometastasis and low CD3+,CD4+ and high CD8+ T-lymphocyte is associated with poor cancer-specific survival.

Objective In order to provide the reasons for local injecting Xiaozhiling to treat malignant tumors. Methods 10 mice were subcutaneosly injected with S 180 Tumor cell to create tumor models and then Xiaozhiling 10ml/kg were intratumorly injected for 3 times.Finally,observation and comparison were made for the pathologic changes under optic and electronic microscopies. Results The results showed that Xiaozhiling had strong antitumor effect on S 180 tumor(adquate rate was 37.3%).Pathalogical exemination revealed that the tumor tissue necrosed obviously,some wesslincataneous cells breaked up, red blood cells extravasation,micro blood clots formed.No changes occurred in the control group. Conclusions Xiaozhiling could be a new drug for clinical treatment of malignant tumor.

Objective: To observe the effect of Ruxiankang (RXK) Capsule (Radix Bupleuri, Poria, Rhizoma Atractylodis macrocephalae, Radix Angelicae Sinensis, Radix Paeoniae Alba, Rhizoma Cyperi, etc.) on mammary gland and sex hormone of rat with mastoplasia. Methods: After benzoate estradiol and progestogen were injected into rats for 30 days the mastoplasia model was made, the model rats were treated with tamoxifen and RXK Capsule for 30days, and injected estradiol at the same time. Results: RXK Capsule and tamoxifen could significantly inhibit HMG rat's mammary gland swell, reduce quantity of lobule alveolus in mammary glands and secretion ( P

Adult ; Aged ; Female ; Gastrointestinal Diseases ; Humans ; Male ; Middle Aged ; Paresthesia ; Pharyngeal Diseases ; Upper Gastrointestinal Tract

Objective To investigate the impacts of γ-ray radiation on gene expressions of peripheral blood lymphocytes in SD rats,and to screen differential expression genes and biological pathways closely related to radiation injury.Methods The differential gene expression of peripheral blood lymphocytes in SD rats was selected by microarray at 6 h after 2 Gy 60Co γ-ray exposure in vitro and in vivo.Bioinformatics analysis of the differentially expressed genes was performed by using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases.Real-time quantitative PCR was applied to verify the screen results of the microarray.Results Fifty-five genes with three times over-expressed level were screened out from the radiation groups both in vitro and in vivo and they were involved in 6 signaling pathways.There were two differentially expressed genes of microarray assay were verified by PCR assay.Conclusions Differential expressed genes in the peripheral blood lymphocytes of SD rats could be induced by γ-ray radiation and they cooperatively contributed to a variety of biological processes.

Adult ; Antineoplastic Agents ; adverse effects ; Comet Assay ; DNA Damage ; Female ; Humans ; Nurses ; Occupational Exposure ; Young Adult

ObjectiveTo explore the relationships of driving coping styles with driving behaviors and road accidents of drivers.MethodsThree hundreds and thirty-seven drivers were randomly surveyed by Driving Coping Questionnaire ( DCQ),Driver Behavior Questionnaire (DBQ).Results ( 1 ) Except avoidance coping style,confrontive coping and emotion-focused coping were correlated positively with the three driving behaviors ( r =0.18 ～ 0.56,P ＜ 0.01 ),and positive appraisal coping had negatively correlations with them (( r =-0.34 ～-0.41,P＜0.01 ).(2)Positive appraisal coping,confrontive coping and emotion-focused coping could predict 33.5％ of error behaviors and 23.5％ of slip behaviors.And confrontive coping and positive appraisal coping could predict 40.2％ of speeding and violation behaviors.(3) Compared with safety drivers,accident drivers had significantly differences in confrontive coping in mild and moderate crashes( t=-2.75,2.80,P＜ 0.01 ).ConclusionDriving coping styles are the important factors influencing drivers' behaviors and road safety.

Objective To explore the characters of non-professional drivers' stress. Methods Two hundred and sixty-four non-professional drivers in Chengdu city were randomly investigated by Driver Stress Inventory(DSI). One hundred and five professional drivers were as control group. Results ①Compared with professional drivers,non-professional drivers scored higher in thrill seeking, cognitive fatigue and aggression (non-professional drivers :4.63±1.49,5.12±1.56,5.65±1.42; professional drivers: 3.60 ± 2.04,3.97 ± 2.02,4.56 ±1.84, P ＜ 0.01 ) ,and lower in hazard monitoring, driving anxiety and physiological fatigue (non-professional drivers:7.04±1.48,6.44±1.50,5.78±1.75; professional drivers:7.89 ± 1.45,6.99 ± 1.28,6.45 ± 2.37, P ＜0.01 ). ②Non-professional drivers' stress had significant differences in gender, age, marital state and driving experiences (P＜0.05). The male drivers scored higher in thrill seeking, aggression and physiological fatigue (male:4.79±1.53,6.78±1.46,5.94 ± 1.80; female:4.32±1.37,5.80±1.37,5.50 ± 1.61, P ＜ 0.05 ). Compared with other age driver groups, the 20 ～29 driver group scored higher in cognitive fatigue (5.44 ± 1.38 ), and lower in hazard monitoring(6.77±1.44) ,driving anxiety (6.03 ±1.46). Compared with unmarried drivers, married non-professional drivers scored higher in hazard monitoring and driving anxiety ,and lower in cognitive fatigue( married drivers:7.23±1.43,6.65±1.48,4.88±1.6; unmarried drivers :6.77±1.47,6.06±1.43,5.59±1.36, P ＜0.05 ). The non-professional drivers who drove more than 20 thousand kilometers scored higher in driving anxiety (6.89±1.41). Conclusion The non-professional drivers' stress isn' t optimistic, and influenced by their gender,age, marital state and driving experiences. Stress management should pay attention to them.

Objective To annalyze the effect of launching blood virus nucleic acid test(NAT) centralization in Chongqing Municipal Blood Center.Methods The various links and key control points in the aspects of the transportation,detection and information system of 32 137 centralized test specimens submitted from 14 basic level local blood stations in Chongqing counties from January 2016 to June 2016 were analyzed.Results Among 32 137 centralized specimens from January to June 2016,55 specimens were rejected for various reasons,the NAT single reactive rate was 5.1‰(164/32 137),which in the Chongqing Municipal Blood Center was 2.3‰(129/55 859)during the same period.The identification detection rate in the basic level blood station was 1.8‰(57/32 137),while which in Chongqing Municipal Blood Center was only 0.6‰(35/55 859).Conclusion The effects of launching centralized NAT have already gradually emerged in Chongqing Municipal Blood Center.The detection ability and level have differences between the laboratory of basic level blood station and blood screening laboratory of blood center.Gradually increasing the centralized test degree conduces to comprehensive improvement of blood detection efficiency and blood saftey.

Objective The paclitaxel loaded solid lipid nanoparticles (PTX-SLNs) were prepared by an ultrasonic-dispersion emulsification technique. The stability of PTX-SLNs was investigated in this study. Methods The technology was preferenced by stability, Zeta potential, particle diameter, and entrapment efficiency as indexes. The doses of lipid materials and coemulsifier, the ultrasonic time, and the ultrasonic power were investigated in detail. Results The optimum prescriptions were definited by one-factor and orthogonal test. The adjuvant: glyceryl monostearate (100/150 mg), Fabaceous Lecithin (100 mg), coemulsifier Pluronic F68∶Tween 80 (2∶1). The samples were sonicated with an energy output of 300 W in 20 min after emulsified at (75?5) ℃. Conclusion The PTX-SLNs are successfully prepared and PTX-SLNs with high stability are fairly dispersed in colloidal solution. This technology has a nice prospect with safety and reliability.