Objective To study the correlation between hand, foot and mouth disease (HFMD) in lung-spleen damp-heat syndrome, syndrome of stagnation and steaming due to damp-heat, and syndrome of stirring wind due to toxic-heat with IL-6, PCT, CRP, CD3+, CD4+, CD8+ and NK cells.Methods Ninety patients with HFMD were randomly selected, dividing into lung-spleen damp-heat syndrome, syndrome of stagnation and steaming due to damp-heat, and syndrome of stirring wind due to toxic-heat, 30 cases for each syndrome. IL-6, PCT, CRP, CD3+, CD4+, CD8+ and NK cells were detected, and the distribution of above indicators in the three syndromes were analyzed.Results Levels of IL-6, PCT and CRP in syndrome of stirring wind due to toxic-heat were significantly higher than those in lung-spleen damp-heat syndrome and syndrome of stagnation and steaming due to damp-heat, with statistical significance (P<0.01); There was statistical significance in absolute value counting of CD3+, CD4+, CD8+ and NK cells among the three syndromes (P<0.01); There was statistical significance in the percentage of CD3+ cells in syndrome of stirring wind due to toxic-heat and lung-spleen damp-heat syndrome (P<0.01) and syndrome of stagnation and steaming due to damp-heat (P<0.05); There was statistical significance in the percentage of CD4+ cells syndrome of stirring wind due to toxic-heat and lung-spleen damp-heat syndrome (P<0.05) and syndrome of stagnation and steaming due to damp-heat (P<0.01); There was statistical significance in the percentages of CD8+ and NK cells in the three syndromes (P<0.01).Conclusion Proinflammatory cytokines, proinflammatory markers, and T cell sub-group related to HFMD in lung-spleen damp-heat syndrome, syndrome of stagnation and steaming due to damp-heat, syndrome of stirring wind due to toxic-heat are not only correlative with morbidity, but also with the severity of diseases and prognosis, which can relatively effectively evaluate the state of diseases and judge prognosis.

Objective: To investigate the effect of miR-144-3p modulating proliferation, migration and apoptosis of liver cancer Huh-7 cells through blocking frizzled class receptor 4 (FZD4)/Wnt/β-catenin pathway and the possible mechanism. Methods: A total of 18 pairs of cancer tissues and corresponding para-cancerous tissues from liver cancer patients, who underwent surgery in Workers' Hospital of Liuzhou City from March 2012 to July 2017, were collected for this study; in addition, hepatic cancer cell lines (Huh-7, SMMC7721 and MHCC97) and human normal liver epithelial cell line THLE-3 were also collected. The expression of miR-144-3p in liver cancer tissues and cell lines was detected by qPCR. MiR-144-3p mimics/inhibitor and FZD4 siRNA were transfected into liver cancer Huh-7 cells; the proliferation, migration and apoptosis of Huh-7 cells were evaluated by CCK-8 assay, Transwell assay, wound healing assay and Annexin V-FITC/PI double staining flow cytometry assay, respectively. The interaction between miR-144-3p and FZD4 was verified by dual-luciferase reporter gene assay. Results: The expression of miR-144-3p was down-regulated in liver cancer tissues and cell lines (P<0.05 or P<0.01). Over-expression of miR-144-3p significantly inhibited cell proliferation viability, migration but induced apoptosis of Huh-7 cells (all P<0.01). Moreover, dual-luciferase reporter gene assay showed that miR-144-3p directly interacted with FZD4 and suppressed its expression. Furthermore, in vitro experiments verified that miR-144-3p targeted FZD4 to suppress the proliferation, migration and promote apoptosis of Huh-7 cells via blocking Wnt/β-catenin pathway (all P<0.01). Conclusion: miR-144-3p inhibits malignant biological behaviors of liver cancer Huh-7 cells via blocking Wnt/FZD4/β-catenin signaling pathway, which may provide potential molecular targets for early diagnosis or treatment of liver cancer.

AIM: To elucidate the effects of losartan on the expression ofmatrix metalloproteinases-2, JNK1/2 and proliferation in cardiac fibroblast. METHODS: Neonatal rat cardiac fibroblasts were cultured. The cells proliferation was determined by MTT. To determine effects of AngⅡ on JNK1/2 activity, cells were incubated (for 0, 2, 5, 10, 30, 60, 120 min) in serum-freemedia with AngⅡ, and the other group fibroblasts were exposed to serum-free media with or without AngⅡ and losartan (AngⅡ 100 nmol/L, AngⅡ 100 nmol/L+losartan 100 nmol/L, losartan100 nmol/L, losartan for 45 min before). Cells protein was collected with MBST buffer. The relative abundance of MMP-2, JNK1/2 and p-JNK1/2 in cells was determined by immunoblotting. The secretion of MMP-2 in media of cell culture was determined by ELISA. RESULTS: AngⅡ increased the proliferation of CFB in a dose-dependent manner, whereas losartan decreased the proliferation of CFB stimulated by AngⅡ in a dose-dependant manner, too (P＜0.05). The relative abundance of JNK1/2 was highest in AngⅡ of the 2-min-stimulated group. AngⅡincreased expression of JNK1/2 and MMP-2 protein (P＜0.05), on the contrary, losartan inhibited JNK1/2 and MMP-2 protein expression.CONCLUSION: AngⅡ induce the increase of proliferation of CFB, expression of JNK1/2 and MMP-2 in CFB, and losartan inhibits these effects of AngⅡ.

Objective To discuss the effect of von willebrand factor (vWF) antibody on idiopathic thrombotic thrombocytopenic purpura(TTP) .Methods 28 cases of idiopathic thrombotic thrombocytopenic purpura were selected in our hospital from January 2011 to June 2013 ,and 30 healthy persons as control group ,ADAMTS13 and vWF of two groups were detected .Results 3 cases of patients were detected with positive vWF antibody ,vWF antibody negative in the remaining patients .3 patients with positive vWF antibody ADAMTS13 antibodies were negative ,ADAMTS13 levels were lower than the normal value .The levels of ADAMTS13 in vWF antibody positive patients was significantly lower than that of vWF antibody negative patients and the control group ,(P<0 .01) .vWF antibody positive patients plasma vWF antibody A was higher than vWF antibody negative and the control group (P<0 .01) .In idiopathic TTP after PE ADAMTS13 antigen increased significantly ,vWF antibody and A levels decreased significantly (P<0 .05) .Conclusion vWF antibodies in idiopathic thrombotic thrombocytopenic plays an important role in the pathogenesis of purpura ,vWF antibody may affect patients ADAMTS13 ,promote vWF complex formation ,effect of disease .

Objective To investigate the effect of myeloid differentiation factor 88 inhibitor ST2825 on the autophagy of THP?1 cells infected by re?combinant mycobacterium smegmatis. Methods The myeloid differentiation factor 88 inhibitor ST2825 was applied on the THP?1 cells infected by recombinant mycobacterium smegmatis,and three groups were defined:the test group with ST2825 treatment,the control group without ST2825 treatment,and the blank group. Autophagosomes were observed under the fluorescence microscope,and the mRNA expression of Beclin?1 gene and Bcl?2gene was analyzed by RT?PCR. Results Compared with the control group,the number of autophagy fluorescent dots in the test group was ob?viously reduced(P<0. 05),and the expression levels of Beclin 1 gene and Bcl?2 gene were declined as indicated by the RT?PCR detection. Con?clusion The myeloid differentiation factor 88 inhibitor ST2825 might inhibit the autophagy of THP?1 cells through interfering the separation of Be?clin?1 and Bcl?2.

A total of 104 marine bacterial strains were isolated from Stelletta tennui around Sanya area of South China Sea by dilution-plate method and were screened for anti-microbial activity on Escherichia coli, Staphylococcus aureu, Pseudomonas fluorescens, Bacillus subtilis, Aspergillus niger, Candida albicans and Pacecilomyces variotii by agar diffusion, paper disc diffusion assay and cell concentration counts methods. It was found that 23 strains, which are 22.2% of the total isolated strains, have anti-microbial activities. Among the 23 strains, A05, A08, A72 and A75 were morphologically, physiologically and biochemically characterized and identified to be the genus Bacillus. At the same time, it was proved that there are positive and negative synergistic effects between or among active strains, e.g., as for A72-75 combination, an obvious enhanced anti-microbial activities on inhibiting Candida albicans and Pseudomomas fluorescens growth was observed than A72 or A75.

OBJECTIVE To investigate infection of Chlamydia trachomatis(CT) and Ureaplasma urealyticum(Uu) in patients with nongonococcal urethritis(NGU). METHODS C.trachomatis was determined by fast immune method of antigen-antibody.U.urealyticum was detected by liquid culture medium. RESULTS Among 320 cases the positive rate of C.trachomatis,and U.urealyticum was 36.25% and 43.75%,respectively.and that of complicated infection was 20.00%.There were significant differences between men and women in C.trachomatis and U.urealyticum infection(P

Objective To investigate the influencing factors for spontaneous bacterial peritonitis (SBP) in patients with acute-on-chronic liver failure (ACLF),and to provide a reference for clinical diagnosis and prognosis evaluation.Methods A retrospective analysis was performed for the clinical data of 667 patients with ACLF who were hospitalized and treated in our hospital from January 2009 to December 2014,and according to the presence or absence of SBP,they were divided into ACLF group(n =232) and ACLF-SBP group(n =435).The general information,laboratory markers,and incidence of complications were compared between the two groups.The t-test was used for comparison of normally distributed continuous data between groups,and the Mann-Whitney U test was used for non-normally distributed continuous data between groups;the chi-square test was used for comparison of categorical data between groups,and a logistic regression analysis was used to identify independent risk factors for ACLF complicated by SBP.Results The comparison of laboratory markers and comorbidities showed that there were significant differences between the two groups in albumin (Alb) (t =-4.110,P ＜ 0.001),alanine aminotransferase (U =-6.653,P ＜ 0.001),aspartate aminotransferase (t =-8.045,P ＜ 0.001),blood sodium (t =-2.879,P =0.006),prothrombin time activity (t =-2.140,P =0.037),international normalized ratio (t =1.453,P =0.042),hemoglobin (t =-3.446,P =0.001),upper gastrointestinal bleeding (x2 =48.252,P =0.002),hepatorenal syndrome (x2 =16.244,P =0.031),and pulmonary infection (x2 =13.564,P ＜ 0.001).The multivariate logistic regression analysis showed that there were significant differences in Alb (OR =1.119,95 ％ CI:1.052 ～ 1.189),platelet count (PLT) (OR =1.035,95 ％ CI:0.755 ～ 1.084),upper gastrointestinal bleeding (OR =1.117,95 ％ C1:0.072 ～ 1.135),and pulmonary infection (OR =2.275,95 ％ CI:0.978 ～ 5.292) (P =0.002,0.038,0.022,and 0.036).Conclusion In the treatment of ACLF patients,risk factors including low Alb,low PLT,upper gastrointestinal bleeding,and pulmonary infection should be prevented,and early diagnosis and intervention of these risk factors helps to reduce the incidence of SBP.

Objective To observe the effect of integrative Chinese and western medicine fast-track rehabilitation in surgery (FTS) for patients during peri-operation period of gynecological laparoscopy. Methods A total of 226 patients receiving gynecological laparoscopic surgery were randomized into observation group and control group, 113 cases in each group. The observation group received integrative Chinese and western FTS treatment, and the control group received routine non-surgery treatment. After operation, time for the first post-operation anal gas evacuation, time for out-of-bed activity, hospitalization time, and incidence of post-operation complications were compared between the two groups. Results In the observation group, time for the first post-operation anal gas evacuation and time for out-of-bed activity were earlier, and hospitalization time was shorter than those in the control group (P<0.05). The incidence of post-operation complications was 0.9%, lower than 4.4% in the control group ( P<0.05). Conclusion Integrative Chinese and western medicine fast-track rehabilitation in surgery shows certain effect on promoting enterokinesia, shortening time for the anal gas evacuation, reducing post-operation complica-tions, promoting post-operation recovery of gastrointestinal function of patients receiving gynecological la-paroscopy .

Objective To explore the value of chemosensitivity assay in vitro on breast cancer.MethodsIn vitro chemosensitivity of 6 species of chemotherapeutic agents applied to 38 cases of breast cancer patients were detected by tissue culture-end point staining-computer image analysis(TECIA).ResultsThe sensitivity to chemotherapeutic agents commonly used in the breast cancer level from high to low was as follow:Doxorubicin(ADM),Paclitaxel(TAX),Vinorelbine(NVB),Cyclophosphamide(CTX),Cisplatin(DDP)and Fluorouracil(FU).ConclusionDrugs sensitivity experiment of cancer in vitro by TECIA has an important value to instruct clinical medication and individual chemotherapy for breast cancer.