2.Application of oral food challenge in the diagnosis of gastrointestinal food allergy in the children
Chinese Journal of Applied Clinical Pediatrics 2016;31(7):557-560
Food allergy is a common illness in children,which is divided into IgE-mediated,non-IgE mediated and mixed-mediated food allergy.Gastrointestinal tract is the mainly affected organ,mostly mediated by non-IgE mechanism.Gastrointestinal food allergy are lack of unified diagnostic criteria currently,whose mechanism is also elusive.Moreover,the double-blind placebo-controlled food challenge is the gold standard for the diagnosis of food allergy.This review focuses on the procedure for the oral food challenge and its application in the diagnosis of gastrointestinal food allergy.
4.The relationship of FOXM1 expression and the clinical pathological factors andclinical response to target-therapy in advanced non-small cell lung cancer
Yan ZHANG ; Wenbin QIAO ; Li SHAN
International Journal of Laboratory Medicine 2017;38(14):1913-1915
Objective To explore the expression of FOXM1 in non-small cell lung cancer(NSCLC) and the relationship between FOXM1 expression and the clinical pathological factors,clinical response to target-therapy in NSCLC remained unknown.Methods A total of 80 NSCLC patients were recruited into this study,FOXM1 expression was assessed by immunohistochemistry and analyzed with the clinical pathological factors and clinical response to target-therapy.Results The positive rate of FOXM1 expression was 41.25%.The positive expression of FOXM1 had no significant difference in patients with different age,gender,cancer staging,smoking history(P>0.05),but had significant difference in patients with different degree of differentiation,lymph node metastasis(P<0.05).Survival time in patients with positive FOXM1 expression was significant shorter than that with negative FOXM1 expression(P<0.05).Conclusion The expression of FOXM1 closely correlated with patients histological differentiation,lymph node metastasis,progress-free survival time in patients with positive FOXM1 expression was significantly shorter than those with negative FOXM1 expression.
5.Serial Analysis of Gene Expression in Parasitological Research
Qiaoli LI ; Zhiming ZHANG ; Zhongdong QIAO
Chinese Journal of Parasitology and Parasitic Diseases 1997;0(06):-
Serial analysis of gene expression (SAGE) is a powerful high-throughput experimental technique that allows rapid, quantitative analysis of global gene expression in eukaryotic organisms. A short sequence taq (10~14 bp),which is defined by an anchoring enzyme site at a fixed distance from polyA tail, contains sufficient information to iden-tify mRNA transcript from which it originates. The taqs are ligated to obtain concatemers that are cloned into a plasmid vector for sequencing. The identification and abundance of mRNA can be observed through bioinformatics and statistical analysis of a given tag. SAGE is not only applied in obtaining global profile of gene expression in a given cell or tissue, but also help identify a set of specific genes to the cellular conditions by comparing the profiles constructed for a pair of cells that are kept at different conditions. This review covers a general introduction of SAGE, its protocol, meth-odological evolution and applications in parasite biology.
6.On the Ultrastructure of Mongolian Milkvetch(Astragalus mongholicus) and a Comparison of Their Isozymes and Lipase
Xiaoling BAI ; Li ZHANG ; Yanxiang QIAO
Chinese Traditional and Herbal Drugs 1994;0(09):-
Astragalus mongholicus with red or green stalks and A.mcmbramaceus with or witho ut down on it leaves were differentiated by comparing their pollen, vein on their leaf surf ace, scanning their stigma and ovary with election microscope and analyzing their isoenzy mes aud lipases
7.Study Progress of Gasotransmitter Hydrogen Sulfide and Cardiovascular Diseases
Journal of Applied Clinical Pediatrics 1986;0(01):-
Hydrogen sulfide is found to be the third gasotransmitter.It exerts extensive biological effects in nerve system,cardiovascular system,gastrointestinal system,and so on.In cardiovascular system,it exerts the function of vasorelaxation,inhibition of vascular smooth mu-scle cells and cardioprotection.It is also concerted with many cardiovascular diseases such as pulmonary hypertension,hypertension,coronary heart disease,atherosclerosis.This review discusses the latest research progress in the significance of hydrogen sulfide in cardiovascular diseases.
8.Perioperative changes in systemic Inflammatory responses in patients undergoing physical cooling during the operation of acute severe cholangitis
Ruidong QIAO ; Zhigang ZHANG ; Ronghua LI
Chinese Journal of Anesthesiology 1994;0(06):-
0.05). The core temperature, levels of TNF-?,IL-6 and IL-8 were lower at t2 and t3 in group Ⅱ than those in group Ⅰ (P
9.Analysis on the Monoamine Neurotransmitters in Hypothalamus of Rat Models of Liver-qi Stagnation Syndrome
Huiyun ZHANG ; Mingqi QIAO ; Li SUN
Journal of Traditional Chinese Medicine 1993;0(02):-
Objective To study the mechanism of liver-qi stagnation syndrome of rat models. Methods The binding method was used to make rat models of liver-qi stagnation syndrome and the quantitative changes of hypothalamus norepinephrine (NE), epinephrine (E), dopamine (DA), and 5-hydroxytryptamine (5-HT) before and after modeling were determined with reversed phase ion pair (RP-IP)-HPLC. Results The level of E, DA and 5-HT was remarkably increased (P
10.Relationship of single nucleotide polymorphism and primary hepatocellular carcinoma
Ke QIAO ; Shu ZHANG ; Zongfang LI
International Journal of Surgery 2008;35(6):399-401
The genesis of primary hepatocellular carcinoma(PHC)is a complicated biology process involve in multiple factors.It has been paid more attention to the genetic susceptibility in the pathogenesis and development of PHC.As the third generation of molecular genetic marker,single nucleotide polymorphism(SNP)becomes a hot topic in the research field of PHC.This article summarized the research evolution of the relationship between the susceptible gene SNP of PHC and PHC susceptibility.