Objective To investigate the risk factors of progressive intracerebral hematoma (PIH) in severe traumatic brain injury (STBI) and provide reference for diagnosis and treatment of STBI.Methods The clinical information of 96 patients of STBI from March 2008 to March 2012 were retrospectively analyzed,the risk factors of PIH were analyzed by the univariate and multivariate Logistic regression analysis.Results Thirty-six patients occurred PIH,the incidence rate of PIH was 37.5％ (36/96).The univariate regression analysis showed that the age,admission GCS scores,injury to the first CT time,brain contusion,subarachnoid hemorrhage,epidural hematoma,subdural hematoma had certain relation with PIH (P ＜ 0.01or ＜ 0.05).Multivariate Logistic regression analysis showed that the age,brain contusion,subarachnoid hemorrhage,epidural hematoma,subdural hematoma were risk factors of PIH (P ＜0.05 or ＜0.01).Conclusion Fully aware of the risk factors of PIH and timely intervention is the key to reduce the morbidity and mortality of STBI.

Objective Though X-ray measurement of metacarpal bones of right hand in children aged 7-12 in Kaschin-Beck disease (KBD) endemic areas in Xinghai County,Qinghai Province,to provide scientific basis for KBD diagnosis and research on growth and development of children in KBD endemic areas.Methods In 2012,277 children aged 7-12 were carried out clinical examination and right hand X-ray photos in KBD endemic areas,Xinghai County,Qinghai Province.Metacarpal bones were measured by electronic digital vernier caliper,and the axis length and width of the metacarpal bones and its metacarpal bone index were calculated.All data were analyzed by SPSS 19.0 software.Results The axis lengths of the metacarpal bones were in an order of 2.metacarpal ＞ 3.metacarpal ＞ 4.metacarpal ＞ 5.metacarpal ＞ 1.metacarpal in 6 different age groups and different genders; the width of 1.metacarpal bone was the widest; the width of 4.metacarpal bone was the smallest,and the rest of metacarpal bones were similar.The metacarpal bone indexes of female [(7.96 ± 0.76),(8.24 ± 0.92),(8.24 ± 0.71),(8.26 ± 0.92),(8.60 ± 1.02),(8.67 ± 0.84)mm] were higher than those of male's[(7.30 ± 0.78),(7.53 ± 0.77),(7.98 ± 0.90),(7.79 ± 0.56),(7.96 ± 0.64),(8.17 ± 0.92)mm,F =4.302,P ＜ 0.05].Conclusion The axis length,width and index of metacarpal bones of right hand in children between KBD endemic areas and no-KBD endemic areas are similar.

Objective By X-ray measurement of metacarpal and phalange bones in the right hand of adult patients with Kaschin-Beck disease in Qinghai Province,to provide anatomic data and information for clinic diagnosis of the disease and application of hand surgery.Methods Fifty-four cases of adult patients with Kaschin-Beck disease,who came from Guide County and Xinghai County in Hainan Prefecture,Qinghai Province,were selected as investigation subjects,then their right hand X-ray film were taken,metacarpal and phalange bones were measured by electronic digital vernier caliper.All data were analyzed with SPSS 19.0.Results The axis length of the metacarpal bones was in the order of:the 2nd metacarpal ＞ 3rd metacarpal ＞ 4th metacarpal ＞ 5th metacarpal ＞ first metacarpal.The axis length of proximal phalange bones was in the order of:the middle finger proximal ＞ ring finger proximal ＞ index finger proximal ＞ little finger proximal ＞ proximal thumb,while the middle,distal phalanx axis length varied greatly.The length of metacarpal and phalange bones of all male was longer than that of female(all P ＜ 0.01).The width of the metacarpal bones was in the order of:the 2nd metacarpal ＞ 3rd metacarpal ＞ 5th metacarpal ＞ 4th metacarpal.The width of the 2nd-5th metacarpal of male and female was (8.57 ± 0.90),(8.25 ± 0.80),(6.84 ± 0.69),(7.70 ± 0.77)mm and (7.40 ± 0.74),(7.36 ± 0.70),(6.00 ± 0.57),(6.62 ± 0.65)mm,respectively,the differences were significant(t =5.24,4.32,4.85,5.55,all P ＜ 0.01).The matacarpal bone index of female[(8.23 ± 0.90)mm] was significantly larger than that of male[(7.61 ± 0.76)mm,t =2.73,P ＜ 0.01].Conclusion X-ray measurement of hand may provide reference information for diagnosis of Kaschin-Beck disease and determination of metacarpal and phalange length in hand surgery.

Objective By investigating newly diagnosed Kashin-beck disease,to understand distribution range,epidemic intensity and prevalence trend of Kashin-Beck disease(KBD)in Xinghai County.Method According to monitoring method on national KBD,we carried on epidemical investigation,clinical examination and X-ray photograph on school children aged 7-13 years in 6 villages from 4 towns in Xinghai County,and hair,grain,water and soil were sampled to detect selenium content using naphthalene fluorescence.Resuit Three hundred and seventy.three school children aged 7-13 years underwent the clinical examination,the prevalence rate was 12.06%(45/373);347 school children were went on X-ray,the detection rate of X-ray was 21.61%(75/347).The selenium content was the lowest in Quanqu Village,Ziketan Town,which were(77.70±42.04),(7.44±6.93)μg/kg respectively;the selenium content Was highest in Cainaihai Village,Qushian Town,which were(103.88±58.57),(29.58±24.11)μg/kg respectively;level of in internal and external environment in 4 KBD region were all low.Conclusion These four region are new KBD region.The prevalence state of KBD is serious,pathogenetic factor of Kashin-Beck disease is very active.

Humans ; Mineral Fibers ; toxicity ; Respiratory Tract Neoplasms ; chemically induced

Adolescent ; Child ; Child, Preschool ; Female ; Herpesvirus 4, Human ; Humans ; Infant ; Infectious Mononucleosis ; diagnosis ; pathology ; virology ; Liver ; pathology ; Male

Female ; Hand-Arm Vibration Syndrome ; diagnosis ; Humans ; Hypothermia ; diagnosis ; Male ; Reference Standards ; Skin Temperature ; physiology ; Young Adult

Objective To observe the effect which the skeletal muscle satellite cells were transplanted on denervated skeletal muscle atrophy so as to provide the experimental data for treating denervated muscle atrophy in vivo. Methods Thirty-two Sprague-Dawley rats weighting 200-250 g were randomly divided into control group and experimental group. Each group included 16 rats, the animal model of denervated gastrocnemius muscles were formed by cutting the right sciatic nerve of rats caused nerve despair about 1 cm. Muscle satellite cells were obtained from the dorsal and lower exterenity muscle of SD rats. Before transplantation, muscle satellite cells being labeled with DAPI (4′-6-Diamidino-2-Phenylin Dole) in vitro. Muscle satellite cells and NS were implanted into denervated skeletal muscle from the two groups. Bilateral gastrocnemius muscles of each rat from the two groups were taken and weighed at second and eighth post-operative weeks respectively. The above muscles underwent anti-actin immunohistochemical and HE staining and muscle cross-sectional area of fiber and the actin content of the rats were measured by the image analysis, the data of which was handled with SPSS software. Results The satellite cells and myofiber with fluorescence were observed in transplantation site. The group of skeletal muscle satellite cells transplanted when two weeks and eight weeks denervated gastrocnemius muscle wet weight remnant rate and muscle cross-sectional areas of fiber remnant rate and muscle actin content were better than in the control group respectively(P

Objective To investigate the prevalence of Kaschin-Beck disease in Wulan and Dulan counties,and to provide basic data in distribution of Kashin-Beck disease.Method In 2015,according to the Diagnosis of Kaschin-Beck Disease (WS/T 207-2010),in 18 villages of Wulan (Tongpu Town,Xiligou Town) and Dulan (Xiariha Town,Xiangride Town) counties,children aged 7-15 and adults over 16 years old who had bone and joint dysfunction underwent epidemiological,clinic and X-ray examination.Results A total of 3 800 children were examined,6 children were found with clinic syndrome of degree Ⅰ Kaschin-Beck disease,the detection rate was 0.16％.By X-ray examination,one student with Kaschin-Beck disease was from other place;other 5 students' X-ray signs were normal.A total of 222 adults older than 16 years old who had bone and joint dysfunction were examined,none of them was found with clinic syndrome of degree Ⅰ Kaschin-Beck disease,the detection rate was 0.Conclusion There is no patient with Kaschin-Beck disease in Wulan and Dulan counties,Qinghai Province.

Objective To observe the protective effects of AG on the myocardial ultrastructure of diabetic rats.Methods STZ-induced diabetic male SD rats were divided into two groups: aminoguanidine(AG group,50 mg?kg~(-1) body weight by daily gavage) and diabetes mellitus groups(DM group).Age-matched normal male SD rats served as normal control(NC group).After 10 weeks of treatment the level of blood glucose was measured and the rats were killed.Cardiac muscle were observed by transmission electronic microscope.Results The changes of cardiac ultrastructure had no significant difference between AG-group and NC-group and were better in AG group than in DM group as following:(1) Myofibril arranged tidily with intact regular edge;(2) Mitochodria were big and normomorph with crests arranging densely,some mitochondria gathered locally;(3)Base membrane of blood vessels didn′t thicken with lumen not narrowed;(4) The collagenous fiber in stroma reduced but amorphous material didn′t reduced. Conclusions The ultrastructure pictures of diabetic cardiomyopathy is inhibited or delayed by AG,which suggests that AGEs may play some role in the development of diabetic cardiomyopathy.