This paper took the charity culture cultivation system of the medical university as the research ob-ject, used the method of empirical research , illustrate how to build the charity culture educational system based on the practice of charitable culture education in Wenzhou medical university .The following:imparting charity culture knowledge , setting up the concept of charity;carrying out thematic education activity , cultivating gratitude;carry-ing out community volunteer service , creating the atmosphere of charity;creating charitable culture brand , building a charity culture education platform were discussed .Furthermore , the practice effect of the charity culture cultiva-tion system was also discussed from educating students , campus culture and promoting social morality .

The study progress of the function of Bid gene, Bid regulate to cell apoptosis, and it can affected by which factors has impulse clinical treatment to develop in recent years. Bid can change into tBid which urge cytochrome C releasing after stimulated by apoptosis signal, and release of cytochrome C induce apoptosis. Apoptosis mediated by Bid is very important to taking place and developing of many diseases. Bid also show dual role that promote apoptosis or proliferation in a few decreas. Studies in influencing factors of Bid mediate apoptosis also provid new ideas to treatment of solid tumor and malignancy hematopathy.

Objective To explore effects of community health education on awareness of hypertension and compliance behavior of hypertensive patients. Methods 300 patients with hypertension aged 18-70 yrs were selected from Hongqiao district of Tianjin in December 2007, and given health education for six-month, including the diagnostic criteria, the main clinical features, common risk factors and treatment-related knowledge, and targeted to help them establishing and maintaining a healthy lifestyle. A questionnaire survey was conducted before (in December 2007) and after (in July 2008) health education and blood pressure was measured. Results Before and after health education, the differences were statistically significant on awareness rates of the diagnostic criteria for hypertension, the main clinical features, risk factors, no cure for hypertension and should take medication, continue with medication even after feeling good (P

OBJECTIVE: To compare the distribution of moxifloxacin in ocular tissue and its pharmacokinetics in rabbit with intravenous and perfusion administration. METHODS: 54 rabbits were divided into intravenous administration group and perfusion administration group (n=27 each). Each group were randomized into 9 sub-groups (n=3). Those groups were given 24 mg?kg-1 moxifloxacin with corresponding adminstration and HPLC was used to detect plasma concentration of moxifloxacin in ocular tissue. 3p97 software was adopted to calculate pharmacokinetic parameters. RESULTS: The Cmax of moxifloxacin by perfusion administration vs. intravenous administration in cornea,aqueous humor, iris-ciliary body,lens and vitreous were (7.09?4.708) ?g?g-1 vs. (2.55?0.382) ?g?g-1; (3.84?1.217) ?g?g-1 vs. (3.71?0.54) ?g?g-1; (5.87?1.93) ?g?g-1 vs. (2.72?0.662) ?g?g-1; (1.36?0.176) ?g?g-1 vs. (0.74?0.396) ?g?g-1; (1.87?0.586) ?g?g-1 vs. (0.87?0.073) ?g?g-1. The AUC0～t of two groups in above tissues were (12.24?1.112) ?g?h?g-1 vs. (9.27?0.559) ?g?h?g-1; (4.71?0.082) ?g?h?g-1 vs. (2.33?0.399) ?g?h?g-1; (13.79?0.814) ?g?h?g-1 vs. (8.04?2.045) ?g?h?g-1; (5.83?0.309) ?g?h?g-1 vs. (3.61?0.41) ?g?h?g-1; (3.45?0.064) ?g?h?g-1 vs. (3.09?0.539) ?g?h?g-1. The pharmacokinetic parameters in perfusion administration group were all higher than that in intravenous administration group (P

The mono-and diacylglycerol lipase(MDGL)-encoding gene(mdlA) was inserted into methanol-inducible expression vector pPIC9K.The linearized recombinant plasmid was uransformed into chromosome of Pichia pastoris GS115 strain by electroporation.Some high-copy transformants(His~(+)Mut~(+)) were picked up by G-418 and conformed by PCR.SDS-PAGE analysis indicated efficient expression of recombinant MDGL.Some enzymatic properties of the recombinant MDGL were also determined.The activity of the recombinant MDGL was up to 325U/mL under the optimal conditions and no activity was detected towards olive oil.The amount of fatty acid produced by the catalysis of recombinant MDGL and triacylglycerol lipase had a increase of 93.5% over triacylglycerol lipase lonely.

Objective:To identify and analyze proliferative diabetic retinopathy with heavy macular edema-related proteins in the vitreous. Mehtods:The vitreous samples of 27 eyes active proliferative diabetic retinopathy with heavy macular edema patients with 9 normal eyes vitreous samples were analyzed by two-dimensional gel electrophoresis then compared the 2-DE map with PDquest to find the disparation proteins spots.The spots were extracted and been enzymolysis and progressing mass spectrogram identification.Then according the results of Peptide Mass Finger printing(PMF)to find the disparation proteins by searching internet using Mascot and Ms-Fit.One sample of the each group was enrolled to identify disparation proteins by Western blot.Results:At least 30 proteins were up regulated or down-regulated by over two folds between two groups.20 protein spots up regulated and 10 down-regulated.Four proteins(apolipoprotein A-IV precursor,Transthyretin precursor,apolipoprotein D,Retinol-binding protein I,cellular)in the 20 spots were expressed significantly differently between the normal and PDR with heavy macular edema patients.The four proteins were up regulated in the diabetic retinopathy with macular edema patients.Conclusions:We constructed vitreous protein profiles for proliferative diabetic retinopathy with heavy macular edema patients and identified four candidate proteins believed to be involved in the pathogenesis of proliferative diabetic retinopathy with heavy macular edema.

Objective:to study the effect of vitrctomy combined with or without cataract extraction.Methods:There were 156 eyes.Team A 86 eyes were operated complicated with cataract extraction(phacoemulsificotion).52 eyes keep post-capsule,34 eyes don't keep post-capsule.Team B 70 eyes simply with vitrectomy.The complications and the incidence of cataract after vitrectomy were observed.Results:The complications were no differents between the two team.The incidence of cataract tampond with C3F8 was 49.03%.The incidence of cataract in patients which tampond with silicone oil was 55.10%.Conclusions:Vitrectomy combined with cataract extraction is effective and safety.Vitrectomy combined can decrease the need of operation in short time.

Objective:To study and evaluate the outcome of Express~(TM) Claucoma Valve Implantation for refractory glaucoma. Methods:Cases series included the eyes of Caucasian patients with refractory glaucoma,operated between 01.2003—10.2004. There were 41 eyes. The Ex-PRESS~(TM) devices were inserted under the sclera flap and into the anterior chamber.If necessary anti-metabolile (MMc or 5-Fu) was used under the sclera flap before injecting the device. Each patient underwent ophthalmic examinations (Vision,IOP,visual field,gonioscopy,cup/disc ratio) before and after the operation. The longest postoperative follow-up period was 12 months. Resultes:The IOP decreased from 24.1?4.62mm Hg pre-operatively to 14.65?1.96mmHg at 26 weeks amt 13.81?1.91mmHg at one year. Success rate was 90.2% (IOP below 20 mm Hg with or without drugs). Complete success was 80% (IOP below 20 mm Hg without anti-glaucoma drops or medications). In 4 eyes IOP was not controlled with eyes drops,and the eves had already been re-operated. 3 cases had an athalamy in anterior chamber but recovered soon by treatment. None of the patients developed infection or irreversible damage to the eye. No case was found to have erosion. Conclusions:the safety and efficacy of the device ureter a sclera flap have been demonstrated in glaucoma surgery in this study.

Hyperoxia, including hyperbaric oxygen (HBO) and high-concentration oxygen (HO), has an important therapeutic effect on various ophthalmological diseases, such as retinal vein occlusion, ischemic optic neuropathy and central serous chorioretinopathy. However, excessive inhalation of oxygen of high-concentration or high atmospheric pressure can cause damage of ocular tissue, in which the lens opacity and retinopathy of prematurity (ROP) are the most common complications following hyperoxia exposure. At present, the pathogenesis of hyperoxia-induced ocular injury is still unclear thoroughly, and it may be related to oxidative stress, lipid peroxidation and inflammation. This article reviewed the current advance in the mechanism and the potential prevention of hyperoxia-induced the lens,retina and other ocular injuries.

Objective To study the effect of cyclic adenosine monophosphate on aquaporin 8 (AQP8) expression and distribution in human amnion-derived WISH cells.Methods Human amnionderived WISH ceils were cultured.The cells were divided into control group and study group at random.The study group was established by exposure to various concentrations of 8-Br-cAMP.Western analysis was used to quantify AQP8 expression levels. RT-PCR was used to quantify AQP8 mRNA expression levels.Immunofluorescence was used to determine the localization of AQP8 in WISH cells.Results With increase of cAMP,AQP8 mRNA and protein expressions significantly increased in WISH cells in vitro.When the concentration of cAMP reached 200 μmol/L,AQP8 mRNA and protein expressions were highest.Incubation with cAMP (200 μmol/L) for 2 hours resulted in a 2-fold increase in AQP8 mRNA level,and incubation for 8 hours resulted in a peak.After incubation for 16 hours AQP8 mRNA level began to descend,and after 24 hours it decreased to baseline.Incubation with cAMP for 8 hours AQP8 protein level began to increase.Incubation for 24 hours resulted in a peak in AQP8 protein level,and after incubation for 48 hours it began to decline.By immunofluorescence microscopy after incubation with cAMP (200 μmol/L) cells,AQP8 labeling in plasma membrane was enhanced and intracellular AQP8 labeling was decreased.Conclusion The cAMP triggers translocation of AQP8 from cytosel to the plasma membrane via vesicle-transporting related protein instead of AQP8 itself,cAMP may upregulate the transcription of target gene protein kinase A.The cAMP may be the critical regulatory medium of AQP8 in WISH cells.