Adult ; Animal Experimentation ; Female ; Hemorrhagic Fever with Renal Syndrome ; etiology ; Humans ; Male ; Medical Laboratory Personnel ; Middle Aged

Adolescent ; Adult ; Carcinoma, Embryonal ; diagnosis ; diagnostic imaging ; Child ; Child, Preschool ; Diagnosis, Differential ; Hemangioma, Cavernous ; diagnosis ; diagnostic imaging ; Humans ; Infant ; Lymphoma ; diagnosis ; diagnostic imaging ; Male ; Middle Aged ; Orchitis ; diagnosis ; diagnostic imaging ; Retrospective Studies ; Seminoma ; diagnosis ; diagnostic imaging ; Teratoma ; diagnosis ; diagnostic imaging ; Testicular Neoplasms ; diagnosis ; diagnostic imaging ; Ultrasonography, Doppler, Color ; Young Adult

Objective To study the role of different subtypes of PKC in the neurite outgrowth of primary cultured neurons from embryonic rat spinal cord. Methods We observed the expression and distribution of protein kinase C(?、?Ⅰ、?Ⅱ、? subtypes) by using immunohistrochemical technique in the neurite outgrowth of the primary cultured neurons from the embryonic rat spinal cord by means of computer image analysis system to measure the lengths of neurites and the areas of bodies. Results The areas of the cultured neuronal bodies increased and the neurites prolonged while the expression of the four subtypes of PKC gradually increased before 7div(day in vitro )during primary cultured neurons growth. The expression both in bodies and neurites of neurons decreased after 7div and the processes gradually shrinked and at the same time bubbles appeared inside the bodies. Relative assay demonstrated that ?Ⅱ subtype was most relative to the outgrowth of neurites( r =0 73, P

BACKGROUND: Logistic analysis of multi-ordered response-variable is used to probe into from another view the interrelationship between lead content in fetus faeces and neurobehavioral development under exposure to low-level lead in uterus.OBJECTIVE: To probe into multi-factors of neurobehavioral development in neonates and the sensibility.DESIGN: The total score of neurobehavior in neonates was taken as dependent variable and 24 indexes were as independent variables, such as induced factors, lead in umbilicus blood and lead in fetus faeces in questionnaire. Logistic progressive regression of multi-ordered response-variables was used in analysis and corresponding factors were screened at level of P=0.10.SETTING: Wuhan University of Science and Technology, Laboratory Room of Occupation Disease and Epidemic disease in Tongji Medical College of Huazhong University of Science and Technology and Occupation Hospital of China First Metallurgical Construction General Company.PARTICIPANTS: Totally 103 full-month borne and healthy neonates were randomized in Department of Gynecology of one occupation hospital in Qingshan District of New-type Industry Area of Huanhan City from January to October 1999 as the objects. The relatives agreed with topic research and questionnaire investigation and they provided neonatal faeces and received neonatal tests on time.off the umbilicus and preserved in freezing in refrigerator at -4 ℃. The faeces in 24 hours after birth was collected and the lead contents of umbilicus blood and faeces were assayed with graphite furnace atomic absorpBehavioral Neurological Assessment (NBNA) was used in examination on the 3rd day after delivery. Simultaneously, the self-designed questionnaire was adopted in the investigation for parturients. The questionnaire involved other possible factors of neonatal neurobehavioral development, including dependent variables, concerning to states of family, society, environment and health that affected neurological development in neonates and lead contents in umbilicus blood and faeces. Scores of neonatal neurobehavior were taken as response variables. Finally, the corresponding factors were screened.MAIN OUTCOME MEASURES: To screen the factors of neurobehavioral development of neonates.RESULTS: Totally 103 cases entered result analysis. Six factors were selected in the model, named pregnant weeks, the month of drug administration in pregnancy, hemoglobin, emotions in pregnancy, lead level in fetus faeces and drug administration.CONCLUSION: Neonatal neurobehavioral development was related to multiple factors. Good nutrient in pregnancy, long pregnant weeks and good emotions in pregnancy benefit neurobehavioral development of neonates. Drug administration during pregnancy is disadvantageous in neonatal neurobehavioral development, especially the medication at the early phase of pregnancy. The increased lead content in neonatal faeces does not benefit neonatal neurobehavioral development.

Objective To investigate demographic factors implicated in the functional maturation of autologous arteriovenous fistula in a population of patients with end-stage renal disease. Methods The data of 335 consecutive patients with end-stage renal disease who were performed autologous arteriovenous fistula from January 2010 to December 2015 were analyzed retrospectively. The parameters affecting autologous arteriovenous fistula functional maturation were screened. Results Overall arteriovenous fistula functional maturation rate was 78.14%(218/279). The arteriovenous fistula functional maturation rate was 68.33%(82/120) in diabetes and 85.54%(136/159) in non-diabetes, and there was significant difference (χ2=11.844, P<0.01). The arteriovenous fistula functional maturation rate was 62.26%(33/53) in hypoproteinemia and 81.86%(185/226) in non-hypoproteinemia, and there was significant difference (χ2=9.648, P<0.01). Diabetes and hypoproteinemia were the risk factors to promote functional maturation (OR=6.003, 8.476). The arteriovenous fistula functional maturation rate was 87.10%(81/93) in calcium channel blockers using and 73.66%(137/186) in non-calcium channel blockers using, and there was significant difference (χ2=6.556, P<0.05). Calcium channel blockers was the protective factor for promoting functional maturation (OR=0.086). Conclusions Diabetes and hypoproteinemia are found to be associated with functional non-maturation, while calcium channel-blocker agents are associated with better functional maturation.

Adult ; Aged ; Female ; Gastrointestinal Diseases ; Humans ; Male ; Middle Aged ; Paresthesia ; Pharyngeal Diseases ; Upper Gastrointestinal Tract

Objective To evaluate the clinical effects and clinical classification of occlusal rehabilitation in elderly patients with abnormal occlusion, and to discuss the diagnosis principles and the practical techniques of the dental prosthesis for occlusal rehabilitation. Methods Forty two elderly patients with abnormal occlusion were treated with occlusal rehabilitation with fixed dentures, fixed-removable dentures and removable partial dentures. Eight patients among them simultaneously had temporomandibular joint(TMJ) disorder. After the clinical procedures including examination, diagnosis, prosthesis design, manufacture, application of occlusal rehabilitation and post-treatment evaluation, the using condition of dentures, patients' satisfaction ratings, TMJ functions and abutment teeth healthy status were examined before and after treatment. Results All the patients were satisfied with their dentures' general functions 1 year after treatment. Compared with the removable partial dentures, the other two types of prosthesis showed better clinical outcomes (χ2=4.15,P<0.05) and compacts on phonation of the dentures (χ2=4.71,P<0.05). In the 8 patients with TMJ disorder, 7 cases were cured completely. The treatment effects of TMJ pain (χ2=0.031, P<0.05)and TMJ click (χ2=0.038, P<0.05)had statistical differences. 30 teeth of the 203 abutment teeth (14.8%) had the problems of periodontal diseases and secondary caries and the incidences of these problems were higher in using removable partial dentures treatment than in the other two methods. Conclusions After the systematic diagnosis and the prosthesis design procedures of occlusal rehabilitation, it is important to choose a proper prostheses for the elderly patients according to their physical and psychological features, which may give the patients satisfactory results.

Adult ; Antilymphocyte Serum ; therapeutic use ; Female ; Graft vs Host Disease ; prevention & control ; Hematopoietic Stem Cell Mobilization ; methods ; Humans ; Immunosuppressive Agents ; therapeutic use ; Male ; Middle Aged ; Transplantation Conditioning ; methods ; Vidarabine ; analogs & derivatives ; therapeutic use

Adolescent ; Adult ; Eye Diseases ; chemically induced ; prevention & control ; therapy ; Fisheries ; Gas Poisoning ; etiology ; prevention & control ; therapy ; Humans ; Hydrogen Sulfide ; poisoning ; Male ; Middle Aged ; Young Adult

Objective To analyze retrospectively the MR features of MELAS patients,in order to improve the early diagnosis of MELAS.Methods MR data of 1 6 MELAS patients confirmed by clinical diagnose and muscle biopsy were retrospectively analyzed. MR features of plain scan(n=1 6),ASL(n=3),MRA(n=1 5),DWI(n=14)and MRS(n=3)were analyzed.Results MRI data demonstrated brain lesions mainly distributed in posterior cerebral hemisphere extending to subcortical area,which was not consistent with the vascular supply territories.The lesions were observed as low signal on Spin-echo sequence of T1 WI,while high signal on T2 WI, FLAIR and DWI.The focal lesions showed increasing Lac peak on MRS.Fifteen of 16 patients underwent MRA and one showed increased arterial branches in lesion zones.Three patients had ASL scanning demonstrating high irrigation in lesion zones which would wander once relapsed.Meanwhile,old lesions showed encephalatrophy and cerebromalacia.All patients’muscle biopsy pathology showed ragged red muscle fibers.Under electron-microscopic,bioblasts were bigger and more than average level and muscle fibers atrophied.Mitochondrial DNA sequence of 3 patients showed mtDNA A3243G transgenation.Conclusion The DWI,MRS and ASL sequences show good ability in MELAS diagnosis and differential diagnosis.