Gestational diabetes refers to the first occurrence or discovery of different degrees of abnormal glucose tolerance during pregnancy, which is one of the most common complications during pregnancy. As the incidence rate increased year by year, the complications of mother and child increased significantly. In recent years, gestational diabetes mellitus has been paid more and more attention. Most of the current researches are about the diagnosis and treatment of gestational diabetes 24 weeks and later, and the theoretical basis for the screening method and treatment of early gestational diabetes is still lacking. With the further understanding of early gestational diabetes mellitus and its adverse maternal and infant pregnancy outcomes, it is imperative to actively carry out the research on the screening and treatment of early gestational diabetes mellitus.

Child ; Chromobacterium ; Female ; Humans ; Sepsis ; drug therapy ; etiology ; microbiology ; mortality

Objective To establish a method to determine the concentration of Danshensu in rats sera by HPLC after rats were fed with Kangxianerhao(KXEH) extract.Metheds Rats were given 0.45g or 0.55g KXEH extract 7 times in 4 days by gavage,and blood were collected from heart of rats at different time.Condition of HPLC: C18A column was used,the mobile phase was consist of a mixture of acetonitrile-H_3PO_4,the detection wavelength was 205nm.Result Concentration of KXEH reached the highest level one hour after administration of KXEH in different doses,which showed that high dose of drug got higher concentration of drug Danshensu in serum than the medium dose.Linear correlation was obtained over the concentration of 76～1520mg/L,r=0.9932.Average recoveries of samples were 98.3%,101.5%,99.3% respectively at different dose of 76mg/L、304mg/Land 1520mg/L,with RSD was 5.9%、2.5%、2.0% respectively.It was proved indirectly that the concentration of KXEH in rat serum after administraion of KXEH extract reached effective concentration in blood.Conclusion It shows that large dose KXEH get higher concentration of Danshensu in blood than the medium dose.The concentration of drug serum is quantitated by HPLC assay.

Objective:To explore expression levels of Tim and TLR gene family in children with different types of mycoplasma pneumonia and their clinical significance.Methods: 56 patients with mycoplasma pneumonia were randomly selected in Ji′nan Maternity and Child Care Hospital from March ,2013 to March,2014.All the patients were divided into bronchopneumonia group ( n=40) and segmental pneumonia group (n=16) according to their X-ray manifestations.Bronchopneumonia group was characterized as dot,nodular increased density shadow with or without reticular ,stripe-like interstitial lesion.Segmental pneumonia was described as segmental or lobar consolidation of lung in X-ray.Meanwhile,30 healthy children were involved in control group with matched age and gender.Peripheral venous blood of all the children in every group was collected and total RNA was extracted .The mRNA of Toll-recepter 2,4 and Tim1,3 was quantified by quantitative real-time PCR (qRT-PCR),and compared between different groups.Results:The expression of mRNA of TLR-2,4 and Tim1 significantly increases in both bronchopneumonia group and segmental pneumonia group compared to healthy control.Meanwhile,the mRNA of TLR-2,4 and Tim-1,3 was significantly elevated among segmental pneumonia group compared to healthy control.In addition,the gene expression of Tim1,3 was higher in segmental pneumonia group than broncho-pneumonia group.Conclusion:The TLR-2,4 and Tim1 gene are over expressed in both types of mycoplasma pneumonia and Tim-1,3 are highly expressed in children with severe infection in lung.The expression alteration of TLR-2,4 and Tim1,3 gene is involved in different types of mycoplasma pneumonia.

ve To establish a method for determination of arsenic in drinking water. Methods The contents of arsenic in water samples were determined directly by graphite furnace atomic absorption spectrophotome-try with masking agent of palladium. The working conditions were defined as wave length of 193.7 nm, slit width of 1.3 nm, current of hollow cathode lamp of 12.0 mA; drying 40 sec at 80℃~140℃ ; ashing 20 sec at 1100℃~1200℃; atomizing 5 sec at 2 300℃; purifying 4 sec at 2 600℃ . Results The linear relationship was 10~200?g/ L ar-senic. The average recovery rates of water samples at concentrations of 50 ?g/ L and 150?g/ L arsenic after being added arsenic standard solutions were 100.7% and 103.6% respectively, and sensitivity reached 10?g/ L. The cor-relation coefficient were 0.9954~0.999 7 obtained from 6 randomly repeated determination of standard curve. The metal ions coexisting in determined water samples didn't interfere the determination of arsenic under the application of common uncoated graphite tube. The 0.2% aluminum didn't disturb the determination of arsenic also. Conclusion This method could be applied in the determination of arsenic in water, especially could be applied in the direct deter-mination of arsenic in more clear water samples.

Female ; Forced Expiratory Volume ; Humans ; Infant, Newborn ; Infant, Premature ; Intensive Care Units, Neonatal ; Male ; Positive-Pressure Respiration ; methods ; Pulmonary Ventilation ; Respiratory Insufficiency ; physiopathology ; therapy ; Treatment Outcome

Objective To investigate the curative effect of electromyographic(EMG) biofeedback therapy on spastic diplegia children with cerebral palsy.Methods42 children were divided into the treatment group(n=22) and control group(n=20) randomly.The treatment group was treated with rehabilitation training plus EMG biofeedback training,while the control group with rehabilitation training solely.Manual muscle test(MMT) was performed before and 3 months after treatment,and the treatment group was assessed by electromyographic apparatus additionally.ResultsAfter EMG biofeedback training,the muscular force of children in the treatment group was higher than those in the control group significantly(P<0.05) and the level of root mean square(RMS) and integrated electromyography(iEMG) assessed by surface electromyography(sEMG) increased significantly(P<0.05).ConclusionEMG biofeedback training can provide a satisfactory treatment for spastic diplegia children with cerebral palsy.Electromyographic apparatus is effective to assess muscular force.

Animals ; Female ; Galactosylceramides ; metabolism ; Graft vs Host Disease ; metabolism ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; T-Lymphocytes ; cytology ; Th2 Cells ; cytology

Adolescent ; Adult ; Aged ; Female ; Humans ; Induction Chemotherapy ; Intercellular Signaling Peptides and Proteins ; metabolism ; Leukemia ; metabolism ; therapy ; Male ; Middle Aged ; Proto-Oncogene Proteins ; metabolism ; Receptor Protein-Tyrosine Kinases ; metabolism ; Young Adult

Objective To observe the clinical efficacy of Wenshen Xiaochuan Paste Recipe (WXPR) in preventing bronchial asthma, and to explore the mechanism. Methods Eighty patients were randomly divided into treatment group and control group. Each group had 40 patients. The control group was treated by conventional western medicine such as inhalation of steroid, and the treatment group was given oral use of WXPR based on the treatment for the control group. Treatment for both groups covered 60 days, and the follow-up lasted 10 months. The clinical outcomes included the clinical efficacy and immune indexes of peripheral blood T lymphocyte subsets CD3+, CD4+, CD8+and CD4+/CD8+ratio, and the serum IgE level. Results (1) After treatment and 12 months later, the scores of asthma control test (ACT ) in the two groups were increased obviously as compared with those before treatment, and there were statistically significant differences (P<0.05 or P<0.01) . The differences of ACT scores between the two groups were insignificant after treatment ( P>0.05) , but were significant ( P<0.05) 12 months later. (2) Within one year after treatment, the frequency of acute asthma attack was decreased in both groups (P<0.05 compared with that before treatment), and the treatment group had better effect on decreasing the attack frequency than the control group (P<0.05). (3) After treatment, the serum IgE, CD4+level, and CD4+/CD8+ ratio were decreased significantly, while CD8+ level was increased in the treatment group, and there were statistically significant differences ( P<0.05 compared with those before treatment). However, the differences of immune indexes in the control group were insignificant before and after treatment ( P>0.05). The improvement of immune indexes in the treatment group was superior to that in the control group ( P<0.05). Conclusion WXPR has good clinical efficacy in preventing and treating bronchial asthma. The mechanism is related with the inhibition of airway inflammation and improvement of immune function probably through regulating T lymphocyte subsets and lowering serum IgE level in asthma patients.