1.Ultrastructural Changes of Human Fetal Myocytes in Different Months
Suxia SHAO ; Lei ZHANG ; Xianglin DUAN ; Ping LU ; Jibiao LI
Progress of Anatomical Sciences 2001;7(1):30-32
Objective To study the ultrastructural changes of 8 human fetus hearts of different months Method by TEM and SEM chemical digestion. Results The shape and ultrastructure of myocytes became complicated gradually with development. Conclusion All the changes are related to function of blood supply of human fetal heart.
2.Influence of katsutoxin and its extract on bone marrow colony-forming unit-granulocyte and monocyte following ~(60)Co gamma ray radiation
Yongkui WANG ; Ping DUAN ; Guoling LI ; Xuefei HAN ; Weihua DONG
Chinese Journal of Tissue Engineering Research 2007;0(11):-
The experiment was performed at Basic Medical College and First Affiliated Hospital, Zhengzhou University from September 2004 to December 2005. Totally 60 Kunming mice were divided into 5 groups randomly: ①blank control group (n =15) and simple radiation group (n =15). The mice were given 0.2 mL sterile saline by intraperitoneal injection. ②antineoplastic polypeptide from Buthus Martensii Venom (APBMV) group (n =10) and APBMV plus radiation group (n =10) received 0.2 mL APBMV according to prepared concentration by intraperitoneal injection. ③Katsutoxin extract Ⅲ plus radiation group (n =10) received 0.2 mL katsutoxin extract Ⅲ by intraperitoneal injection every other 5.5 hours for 7 days. After 24 hours from the last injection, the mice were endured 60Co g ray radiation (80 cm, 7.5 Gy irradiation dose, 0.27 Gy/min dose rate). Then katsutoxin extract Ⅲ was given same as above for 7 days. Then bone marrow was extracted to be cultured to colony-forming unit-granulocyte and monocyte (CFU-GM). The findings showed that colony amount of APBMV plus radiation group and katsutoxin extract Ⅲ plus radiation group was obviously more than that of simple radiation group [(32?5),(27?3),(2?1)pieces/well,P
3.Ultrasonic measurement of vasodilation in cerebral infarction patients
Shanchun ZHANG ; Luxiang CHI ; Rui LI ; Ping CHEN ; Lingmin DUAN
Journal of Third Military Medical University 2003;0(20):-
Objective To investigate the bilateral flow-mediated dilation of brachial artery (BA-FMD) and nitroglycerin mediated dilation (BA-NMD) in patients with cerebral infarct, and to find out if the vasodilation of internal catotid artery after sublingual administration of nitroglycerin can be detected as a more direct way to reflect the vasodilation in patients with cerebral vascular diseases. Methods High resolution ultrasonic system was used to measure 13 patients with acute cerebral infarction. The diameter of brachial artery, internal carotid artery and common carotid artery was measured at relaxation, reactive congestion in the brachial artery and after sublingual administration of nitroglycerin. Meanwhile, the intima media thickness (IMT) and plaques of carotid artery was detected. Results Paralysis did not lead to differences in vasodilation of brachial artery (r=0.52, P=0.07). There was a correlation between ICA-NMD and BA-NMD (r=0.83, P=0.01). Conclusion There were no significant differences between brachial vasodilation in paretic and non-paretic limbs of patients with acute cerebral infarction. Ultrasonic measurement of ICA-NMD can be a more direct measure of the vasodilation of patients with cerebral infarction.
4.The Explore of English Teach Model Based on the Capability Cultivation of Medical Postgraduate Students
Li SHANG ; Baosheng XIE ; Lu LIU ; Ping DUAN
Journal of Kunming Medical University 2006;0(06):-
Recently,the traditional English teaching model can not match the social demand to the medical postgraduate students.This paper analyses the effective factor of English teaching and learning.We hope to explore a new set of capability cultivation basal English teaching model of medical postgraduate students through editing teaching outline,evaluating demand,implementing classified teaching,giving medical electives and improving examination means.
5.Factors Associated with Fungal Infection Following Pancreatoduodenectomy
Shigang DUAN ; Ping CHEN ; Ying LI ; Jiming DING ; Lin ZHANG
Chinese Journal of Bases and Clinics in General Surgery 2003;0(03):-
Objective To investigate the relevant factors for fungal infection following pancreatoduodenectomy and offer the theoretical foundation for preventing the emergence of complications after operation. Methods Medical records from 562 consecutive patients who underwent pancreatoduodenectomy in this hospital from 1995 to 2005 were retrospectively reviewed by using single factor and non-condition Logistic regression analyse. Results ①Seventy-eight patients (13.9%) developed invasive fungal infection. The most frequently isolated fungal were Candida albicans accounted for 67.0%, and followed by Candida glabrata, Candida papasilosis and Candida tropicalis and gastrointestinal tract was the most common infection site, followed by respiratory tract, abdominal cavity. ②Fungal infection occurred significantly more often in patients with the length of time in parenteral nutrition, antibiotic use or abdominal cavity complications. Conclusion The most common infection site and isolated fungal associated with pancreatoduodenectomy were gastrointestinal tract and Candida albicans. Abdominal cavity complications such as pancreatic fistula, biliary fistula and abdominal infection and extended use parenteral nutrition and antibiotic are the most important factors leading to invasive fungal infection after pancreatoduodenctomy. Eliminating the various risk factors will decrease the incidence of fungal infection.
6.Expression and significance of CD105 in endothelial cells of primary hepatocellular carcinoma
Ying LI ; Ping CHEN ; Jin ZHU ; Shigang DUAN
Journal of Third Military Medical University 2003;0(07):-
0.05). Conclusion CD105 overexpression may play a crucial role in early recurrence of HCC in patients after operation. The expression of CD105 mRNA in the tumor peripheral tissue was much higher than in the tumor center and normal liver tissue, which indicated CD105 was related to early recurrence.
7.Castleman's disease of chest wall complicated by follicular dendritic cell sarcoma/tumor: report of a case.
Zhan-ping CHANG ; Song-lin LIAO ; Yan JIN ; Qiu-ping SONG ; Li-jiang DUAN
Chinese Journal of Pathology 2007;36(6):430-431
Castleman Disease
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complications
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metabolism
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pathology
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surgery
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Dendritic Cell Sarcoma, Follicular
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complications
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metabolism
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pathology
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surgery
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Follow-Up Studies
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Humans
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Male
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Middle Aged
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Receptors, Complement 3b
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metabolism
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Thoracic Diseases
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complications
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metabolism
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pathology
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surgery
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Thoracic Wall
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Vimentin
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metabolism
8.Effect of Jiuqiang Naoliqing on the TXA_2 and PGI_2 level in spontaneous hypertension rat plasma
Xiang-ying KONG ; Ping-ping ZUO ; Zhi-meng LI ; Jinhong DUAN ; Lingna KONG ; Shunling DAI
Chinese Journal of Rehabilitation Theory and Practice 2004;10(9):518-519
ObjectiveTo investigate the effect of Jiuqiang Naoliqing(JNQ) on the TXA2 and PGI2 level in spontaneous hypertension rat (SHR) plasma.MethodsThe plasma was separated after the SHR and Wistar rats were treated with JNQ at the dose of 0.133g/kg,0.265g/kg,0.530g/kg and 1% carboxymethyl cellulose respectively for 5 weeks. The level of TXB2 and 6 keto PGF1α ,stable metabolin of TXA2 and PGI 2,in SHR plasma was tested by radioimmunoassay.ResultsThe level of TXB2 and the ratio of TXB2/6 keto PGF1α (T/P) in SHR plasma increased significantly(P<0.01),and there was no significant difference in the concentration of 6 keto PGF1α between Wistar rats and SHR plasma(P>0.05). JNQ could increase the generation of 6 keto PGF1α and decrease the level of TXB2 and T/P in SHR plasma after treated with different dosages for 5 weeks.ConclusionJNQ may improve the balance between TXA2 and PGI2 in SHR plasma.
9.Research of interference Livin gene inhibits growth of QBC939 cell
Shengxiong CHEN ; Ping CUI ; Chen LI ; Ming HONG ; Shaoyou LI ; Lele DUAN
Chinese Journal of Hepatobiliary Surgery 2011;17(7):580-583
Objective To study the effect of transfection of livin antisense oligodeoxynucleotide (Livin ASODN) on Livin mRNA and Livin protein expression and proliferation of QBC939 cells.Methods Livin ASODN was transfected into cell line QBC939 by LipofectamineTM 2000. Fluorescence microscopy was used to observe the ASODN transfected cells and to calculate the rate of transfection.to measure Livin mRNA and Livin protein expression by RT-PCR and immunohistochemistry and con-focal laser scanning microscopy after the transfection. Changes in cell proliferation were detected by MTT. Results The highest efficiency was at 24 hours after 500 nmol/L Livin ASODN transfection.The results of MTT showed that the inhibition of cell proliferation of QBC939 cells was most obvious at 60 hours after Livin ASODN transfection (P<0. 05). The level of Livin mRNA and Livin protein expression in the ASODN group was obviously lower than that in the control group (P<0. 05).Conclusion The transfection of Livin ASODN inhibited Livin gene and Livin protein expression, and obviously inhibited the proliferation and depressed the vitality of QBC939 cells.
10.Research progress in receptors involved in rotavirus infection.
Xin MA ; Dan-Di LI ; Xiu-Ping LI ; Zhao-Jun DUAN
Chinese Journal of Virology 2014;30(3):303-309
Rotaviruses, which are recognized as one of the major etiological agents among infants and young children with diarrhea, consist of three concentric layers of protein capsid with the enclosed double-stranded RNA genome. Rotaviruses infect host cells mainly by identifying the specific receptors on cell surfaces and binding to them. Therefore, receptors are important factors for viruses infecting cells. So far, there have been many receptors found to be involved in rotavirus infection, including sialic acid, integrin, Toll-like receptor, and blood group antigen. This article provides an overview of receptors involved in rotavirus infection.
Animals
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Humans
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Receptors, Virus
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genetics
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metabolism
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Rotavirus
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genetics
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physiology
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Rotavirus Infections
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genetics
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metabolism
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virology