Objective To investigate the effects of Lycopene ( LP) on immune function of ovarian cancer rats′and its mechanism .Metho ds Sixty ovarian cancer rat models made by injecting ovarian cancer cell line NUTU-19 were divided into five groups randomly:model group,Cisplatin group(2 mg/kg)and LP(40,20,10 mg/kg)group,n =12;the LP and Cisplatin were given by intraperitoneal injection ,once every two days for 5 times.The rats general states and tumor growth conditions were observed ,the tumor tissue morphological changes was observed by H&E staining;the tumor weight ,inhibition rate,spleen lymphocyte transformation rate were de -tected;and the content of IL -2 and TNF-αin serum were detected .The present of CD4 +,CD8 +in blood were detected and the ratio of CD 4 +/CD8 +was calculated .Results The food situation of the rats in LP groups was improved,tumor was reduced,activity and hardness were poorer ,necrosis presented ,tumor cell shrinkage and oth-er pathological morphological changes appeared ,especially the rats in LP 40 mg/kg group .Compared with model group,the tumor weight in LP(40,20 mg/kg)groups was decreased,the inhibition rate and the spleen lymphocyte transformation rate were increased;the contents of IL-2 and TNF-αwere decreased;the CD8+present was de-creased,while the CD4 +present in LP 40 mg/kg group was increased,the ratio of CD4 +/CD8 +was increased. All of the difference above were significant (P<0.05,P<0.01).Conclusion LP can effectively improve the immune function of ovarian cancer rat;which perhaps relates to its effects of reducing the inflammatory cytokines levels and increasing the ratio of CD 4 +/CD8 +.

Aged ; Anthracosis ; complications ; diagnostic imaging ; Humans ; Male ; Middle Aged ; Pulmonary Emphysema ; complications ; diagnostic imaging ; Tomography, Spiral Computed ; Trachea ; diagnostic imaging ; pathology

OBJECTIVETo investigate the chemical constituents of the dried buds of Lonicera confusa.

METHODChromatography and spectral analysis were respectively used to isolate and identify the constituents.

RESULTSeven compounds were isolated from the dried buds of L. confusa, and identified as rutin, quercetin, luteilin-7-O-beta-D-galactoside, lonicerin, chlorogenic acid, beta-sitosterol and tetratriacontane.

CONCLUSIONRutin was isolated from the genus for the first time, and the others were isolated from the species for the first time.

Chlorogenic Acid ; chemistry ; isolation & purification ; Flowers ; chemistry ; Lonicera ; chemistry ; Plants, Medicinal ; chemistry ; Quercetin ; chemistry ; isolation & purification ; Rutin ; chemistry ; isolation & purification ; Sitosterols ; chemistry ; isolation & purification

Objective To investigate the effective therapic methods of the perioperative vasovagal reflex(RV)in patients with atrial arrhythmias(AAs)underwent by catheter ablation .Methods The clinical data in the patients with atrial arrhythmias(AAs) complicated by vasovagal reflex(RV)were retrospectively analyzed during the perioperative procedure of catheter ablation guided by three dimensional mapping system CARTO ;the clinical data were compared between patients with RV and without RV .Results A total of 535 patients with AAs underwent catheter ablation guided by three dimensional mapping system were collected from Janu‐ary 2010 to December 2012 ,and 35 cases of intraoperative RV were found .Compared by patients without RV ,the patients with RV had more elderly cases and the cases of hypertension ,coronary heart disease and diabetes .The RV occurrence was also associated with the fasting ,painful stimuli ,operation time and skill .Conclusion The perioperative RV often is occurred in the cases of high risk and have the precipitating factors ,and the good prognosis could be obtained by the detect and treatment as early as possible .

Objective:To explore the risk factors for esophageal stricture after endoscopic resection (ER) of large-area early esophageal cancer (≥3/4 circumferential mucosal defect).Methods:A total of 63 cases of large-area early esophageal cancer treated with ER in the Digestive Endoscopy Center of the First Medical Center of PLA General Hospital from May 2009 to April 2016 were included in the retrospective analysis. They were divided into stricture group (32 cases) and non-stricture group (31 cases) according to the occurrence of postoperative esophageal stenosis. T-test or Chi square test was conducted to compare the indicators between the two groups. Indicators of P<0.05 and potential indicators from the clinical perspective were included in multivariate logistic regression analysis. Results:Univariate analysis showed that the length of lesion, the degree of mucosal defect around the wound and the injury of muscularis propria were associated with esophageal stricture after ER ( P<0.05). The above 3 indicators were included in the multivariate logistic regression analysis, together with 3 other indicators, i. e. preventive measures for stenosis, pathological type, and en bloc resection. The results showed that more than 7/8 circumferential mucosal defect around the wound (VS 3/4-<7/8 circumferential: P=0.028, OR=0.317, 95% CI:0.114-0.884) and no preventive measures ( P=0.002, OR=0.153, 95% CI:0.046-0.512) were independent risk factors for esophageal stricture after ER of large-area early esophageal cancer. Conclusion:Circumferential mucosa defect≥7/8 is the main factor leading to esophagus stricture after large-area early esophagus carcinoma. And appropriate preventive measures can effectively reduce the incidence of postoperative stenosis after ER.

Objective To explore the diagnostic value of prostate specific antigen (PSA),compound prostate specific antigen(cPSA),and cPSA / tPSA ratio in prostatic cancer(PCa).Methods Two hundred nineteen people,aged 62-68,were divided into three groups according to the clinical diagnosis,including PCa group(n=73),benign prostatic hyperplasia(BPH) group(n=76) and control group(n=70) composed of healthy people.The various indicators,including tPSA,cPSA and cPSA/tPSA were measured by Advia Centaur CP chemiluminescent analyzer.Results tPSA,cPSA and c/t significantly increased in PCa group compared with those in BPH or control group (P<0.05).ROC analysis indicated that if the tPSA cut-off values was19.89 μg/L,the sensitivity,specificity,the area under the curve (AUC) and Youden index would be 94.5%,97.9%,0.96 and 0.924 respectively.If the cut-off value of cPSA was 13.14 μg/L,the sensitivity,specificity,the area under the curve(AUC) and Youden index would be 95.9%,91.8%,0.98 and 0.877 respectively.The chi-square test showed that there is no correlation between tPSA,cPSA and pathology diagnosis(χ2=102.036、151.409,P<0.05).Conclusion PCa or PCa treatment relapse should be highly suspected when the concentration of tPSA or cPSA in patients reached 19.89 μg/L and 13.14 μg/L,the patients should be reminded for treatment timely.

Objective:To examine a nomogram model for individualized prediction of the risk for recurrence of early gastric cancer(EGC)in elderly patients undergoing endoscopic submucosal dissection(ESD).Methods:This was a retrospective cohort study, with a total of 3 987 elderly EGC patients who underwent ESD treatment between January 2000 and December 2016 after admission to the gastroenterology department of our hospital.Twenty-eight relapsed patients with complete clinicopathological data and follow-up data were selected as the relapse group, and 276 non-relapsed patients were selected as the control group.General data of all patients were collected and a logistic regression analysis was performed to analyze independent risk factors for the recurrence of EGC in patients after ESD.A corresponding nomogram risk prediction model was established by using the R software.Results:Among the 3 987 elderly EGC patients, 29 relapsed after an average follow-up of 2.7 years, and the recurrence rate was 0.73%(29/3 987). The differences in baseline data such as age(≥75 years old), lesion size(≥3 mm), T stage and lymph node metastasis between the recurrence group and the control group were statistically significant(11 cases or 39.3% vs.171 cases or 62.0%, 19 cases or 67.9% vs.111 cases or 40.0%, 9 cases or 32.1% vs.153 cases or 55.4%, 19 cases or 67.9% vs.102 cases or 39.0%, P<0.05). Logistic regression analysis showed that age over 75 years( OR=2.128, 95% CI: 1.373-3.624), T stage( OR=1.763, 95% CI: 1.079-2.934), lesion size≥3 mm( OR=2.604, 95% CI: 1.363-4.217), and lymph node metastasis( OR=2.871, 95% CI: 1.425-5.639)were independent risk factors for the recurrence after ESD in EGC patients( P<0.05). The nomogram model was established based on the above risk factors, and the validation results showed that the predicted value was basically the same as the actual measured value and had good predictive performance.The internal validation results showed that the consistency index was 0.817(95% CI: 0.722-0.941), suggesting that the model had a high accuracy and discrimination. Conclusions:Before ESD for elderly EGC patients is performed, factors such as age, tumor size, T stage and lymph node metastasis should be fully considered to comprehensively evaluate the recurrence rate of EGC after the procedure.This predictive model can improve the diagnostic efficacy of postoperative recurrence and has high clinical value.

Aged ; Coal Mining ; Humans ; Male ; Middle Aged ; Pneumoconiosis ; complications ; diagnostic imaging ; Pulmonary Heart Disease ; diagnostic imaging ; etiology ; Sensitivity and Specificity ; Tomography, Spiral Computed ; methods

Objective To study the immunogenicity of human embryonic stem cells(hESCs)and the derived neural stem cells(NSCs)in vitro.Methods The constitutive expression of human leucocyte antigen(HLA)Ⅰ and Ⅱ in hESCs and the NSCs derived from these hESCs were detected by flow cytometry (FCM), as well as the expression of HLA-Ⅰ,Ⅱin NSCs induced by 30 ng/ml recombination human interferon-?(IFN-?).Meanwhile, the NSCs before and after induction of IFN-? were co-cultured with peripheral blood lymphocyte obtained from healthy person.Lymphocyte proliferation standing for the immunoreactivity of NSCs was then investigated.Results The hESCs slightly expressed HLA-Ⅰ(6.18%) and hardly any HLA-Ⅱ before differentiation.However, the NSCs expressed more HLA-Ⅰ(23.56%)as well as HLA-Ⅱ(1.28%, 1.73%)than the hESCs did.Both HLA-Ⅰ(46.43%)and HLA-Ⅱ(8.73%, 10.57%)expressed by the NSCs after they were induced by IFN-? were up-regulated.Conclusions hESCs express certain level of HLA-Ⅰ molecules but do not constitutively express HLA-Ⅱ molecules.The derived NSCs express heavy HLA-Ⅰ and a little HLA-Ⅱ, when treated by IFN-? they can inducibly up- regulated both molecules.The NSCs derived from HESCs are of immunogenicity, which induce rejection aiming at HLA-Ⅰ molecules or even at HLA-Ⅱ molecules when the host is inflammative or under stress, which can result in a failure of cellular transplantation.

OBJECTIVEThis study aims to conduct a survey of the oral health status of disabled individuals in Liangshan Autonomous Prefecture in Sichuan province. This study was also conducted to prepare caries prevention planning in the region.

METHODSOn the basis of the Oral Health Surveys: Basic Methods of WHO and the Third National Oral Health Epidemiological Sample Survey Scheme, we investigated caries infecting disabled individuals who live in one big city (Xichang city), three counties (Bhutto county, Muli Tibetan autonomous county, and Huili county), and 46 towns and villages of Liangshan Autonomous Prefecture in Sichuan province, by multi-stage stratified and cluster sampling.

RESULTSAmong 3,439 disabled individuals, 2,085 were males and 1,354 were females; among these individuals, 815 live in the city and 2,624 live in rural areas. Furthermore, 2,177 were Han natives and 1,262 were Yi natives (ethnic). The caries prevalence rate and mean DMFT of disabled individuals in Liangshan Autonomous Prefecture in Sichuan province were 87.1% and 9.53, respectively. In Yi, the caries prevalence rate and mean DMFT of disabled individuals in Liangshan Autonomous Prefecture in Sichuan province were 85.8% and 9.93, respectively; in Han, the caries prevalence rate and mean DMFT of disabled individuals in Liangshan Autonomous Prefecture in Sichuan province were 87.9% and 9.29, respectively. No significant difference was found in the two groups of native (P > 0.05). A very low filling rate of 0.2% was also recorded.

CONCLUSIONDisabled individuals from Liangshan Autonomous Prefecture in Sichuan province exhibited a high prevalence of caries in permanent teeth. The oral health status of this special group of disabled individuals should be provided intensive care.

China ; Dental Caries ; Female ; Humans ; Male ; Oral Health