2.Design and application of auxiliary recovering device after retinal detachment operation
Lilan LI ; Lianhong NI ; Xiaoxin WU
Chinese Medical Equipment Journal 2017;38(5):45-47
Objective To develop an auxiliary recovering device for prone position nursing after the retina vitrectomy in order to improve comfort and treatment compliance.Methods The device was made of stainless steel,and consisted of a base,pulleys,supporting rods and a placing case.Totally 40 patients receiving retinal detachment operation were divided into an experimental group and a control group.The patients in the experimental group applied the auxiliary device and the ones in the control group underwent conventional nursing,and then a 2-week observation was executed on the prone time,overall satisfaction and adverse response after the operation.Results The device behaved well in prone time,patient comfort and satisfaction,and the experimental group gained advantages over the control group in prone time,relieving muscle pains,arthralgia,poor breath,anxiety and insomnia.Chi-square test proved the experimental group had the patient satisfaction significantly enhanced when compared with the control group (P<0.05).Conclusion The device can be used for auxiliary nursing after retinal detachment operation with simple structure,easy operation and high comfort,and thus is worthy promoting practically.
4.LOCALIZATION OF PROTEIN KINASE C? SUBUNIT IN RAT NERVOUS SYSTEM
Tongshang NI ; Shengxi WU ; Yunqing LI
Acta Anatomica Sinica 1954;0(02):-
Objective To observe the distribution of protein kinase C? subunit(PKC?) in the rat nervous system. Methods Immunocytochemical staining for PKC? by using specific antibody against PKC? was performed. Results Intensely and densely stained PKC? immunoreactive neurons were mainly observed in the cerebral cortex,hippocampus,amygdaloid complex,cerebellar cortex, ventral and dorsal cochlear nuclei, caudal spinal trigeminal nucleus and the superficial layers of the spinal dorsal horn.Conclusion\ PKC?\|containing neurons are widely located in the rat nervous system.These results provide morphological evidence for the functional roles of PKC? in the signaling transduction of the nervous system.\;[
5.Preparation of recombinant alpha-virus
Wanling LI ; Bing NI ; Yuzhang WU ;
Journal of Third Military Medical University 2003;0(10):-
Objective To explore a new simpler method for the preparation of recombinant alpha virus as a novel vaccine at the DNA level. Methods Plasmids expressing ? gal protein and helper plasmids were transfected into BHK cells. Virus in culture supernatant of the transfected BHK cells were collected and purified and used to infect BHK cells in vitro to identify the expression of target gene and the titre of the recombinant virus. Results Recombinant virus with high titre, prepared by this method, could be expressed well in mammalian cells in vitro . Conclusion High titre recombinant alpha virus can be produced at the DNA level and this method can be applied for vaccine preparation and gene therapy.
6.Studies on genetic diversity of three Tibetan herbs.
Liang-hong NI ; Zhi-li ZHAO ; Jin-rong WU ; Bo XIONG ; Jia-ni LU ; Dorje GAAWE
China Journal of Chinese Materia Medica 2015;40(19):3883-3888
The genetic diversity of three Tibetan herbs, i. e., Sang-Di, E-Dewa and Ye-Xingba (Tibetan names), was studied based on the field collection, specimen identification and DNA sequence analysis. Swertia hispidicalyx, Gentiana lhassica and Scrophularia dentata, as the original plants of the three Tibetan herbs, were collected and identified. The regions of ITS, matK, rbcL, rpoC1, trnL(UAA), psbA-trnH, atpB-rbcL, trnS (GCU)-trnG(UCC), rpl20-rps12, trnL(UAA)-trnF(GAA) and nadl 2nd intron were amplified and sequenced. The ITS regions of S. hispidicalyx and S. dentata were cloned and sequenced, and the sequences were classified into different genotypes. All the sequences were analyzed and compared with those of closely related species. Our studies may provide reference for the genetic diversity analysis and molecular identification of the three Tibetan herbs.
Genetic Variation
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Gentiana
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classification
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genetics
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Phylogeny
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Plant Proteins
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genetics
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Plants, Medicinal
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classification
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genetics
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Scrophularia
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classification
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genetics
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Swertia
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classification
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genetics
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Tibet
7.The feasibility of building the oblique lumbar interbody fusion corridor: A CT image study
Haichao XU ; Zhenhua FENG ; Xiaobin LI ; Aimin WU ; Wenfei NI
Chinese Journal of Orthopaedics 2017;37(16):1021-1028
Objective To determine the radiographic feasibility of oblique lumbar interbody fusion (OLIF) corridor to treat lumbar disease at each lumbar disc level,including the corridor's numerical value and the influence of diaphragmatic crura and aorta abdominalis.Methods A retrospective CT study was conducted on 110 patients (including 69 males and 41 females,average age 47.95 years,range 16-83 years) that continuously collected and analyzed in the PACS system.The oblique corridor was defined as the area between the left lateral border of the aorta abdominalis(or iliac artery) and the right lateral border of the left psoas.The distances and angles of L1-2,L2-3,L3-4 and L4-5 levels were measured.Whether the change of diaphragmatic crura and aorta abdominalis affected the building of the corridor was also observed.Results The mean distances of oblique corridor to the levels of L1-L5 discs were:L1-2 15.90 mm,L2-3 14.82 mm,L3-4 17.57 mm,L4-5 11.16 mm.At the levels of L1-2 and L3-4,all of the images could build the corridor.But there were only 97.27% images allowing operation at both L2-3 and L4-5,and the other 3 cases couldn't build the corridor since the aorta abdominalis was very close to psoas,and the distance was almost 0 mm.The max mean distance was 36.79 mm at L3-4 level.The mean angles were:L1-2 36.98°;L2-3 37.76°;L3-4 40.96°;L4-5 37.85°.The significant difference was at L3-4,ranged from 13.09 to 61.93°.The level of the aortic bifurcation was from the lower third of the L3 vertebral body to the middle third of the L5 vertebral body.The levels of left diaphragmatic crura's ending point in the lumbar was divided into four groups:1) Group L1 vertebral body level:the level at L1 vertebral body and above,5 cases (4.55%);Group L1-2 disc to L2 vertebral body level:at L1-2 disc and L2 vertebral body,67 cases (60.91%);Group L2-3 disc to L3 vertebral body level:at L2-3 disc and L3 vertebral body,36 cases (32.72%);Group L3-4 disc to L4 vertebral body level:at L3-4 disc and L4 vertebral body,2 case (1.81%).Conclusion The OLIF corridor can be built successfully at L1-2 and L3-4.However,it may be difficult at L2-3 and L4-5 for some patients due to the aorta abdominalis which is too close to psoas.The angles of L1-L5 levels were similar.While the left diaphragmatic crura was mainly impact the corridor insertion at L1-2 and L2-3.And the level of the aortic bifurcation was mainly located at the upper endplate of L4 to the L4-5 disc (87%).
9.Correlation between blood lipid metabolic disorder and insulin resistance in the non-diabetic first degree relatives of type 2 diabetic pedigrees
Quanmin LI ; Suhua ZHANG ; Wei REN ; Yinxing NI ; Jing WU
Chinese Journal of Tissue Engineering Research 2005;9(7):156-157
BACKGROUND: Metabolic disorder of blood lipid can be observed in the non-diabetic first-degree relatives.OBJECTIVE: To explore the relationship between the level of blood lipid and insulin resistance in members of type 2 diabetic pedigrees.DESIGN: A retrospective case control study of the non-diabetic first-degree relatives of type 2 diabetic families.SETTING: The department of endocrinology of a university hospital.PARTICIPANTS: This study was carried out in the Department of Endocrinology of the First Affiliated Hospital of Chongqing Medical College from June to November 1999. A total of 125 non-diabetic first-degree relatives of type 2 diabetic pedigrees in Chongqing were recruited and divided into hyperlipemia group and normal control group according to their blood lipid level.INTERVENTIONS: Body height, body mass and blood pressure of all the subjects were measured. Their cubital venous blood samples were also obtained for determination of the levels of fasting plasma glucose (FPG), fasting insulin(FINS), fasting C-peptide(FCP), triacylglycerol(TG), total cholesterol(TC), low-density lipoprotein-cholesterol (LDL-C), and high-density lipoprotein-cholesterol (HDL-C).homeostasis model assessment insulin resistance(HOMA-IR) between the two groups.RESULTS: The level of FINS was markedly higher in the hyperlipemia group [(12.92 ±5.26) μU/L] than in the control group[(9.15 ±4.66) μU/L]( t = 2. 17, P < 0.05) . HOMA-IR was also significantly higher in the hyperlipemia group [ (2.94 ± 1.25 ) ] than in the control group [ (2.01 ± 0.94) ]( t = 2.70, P < 0.01 ) . In the hyperlipemia group, HOMA-IR was positively correlated with TC, TG and LDL-C ( r = 0. 34, 0. 78, 0.42), with TG most closely correlated( r = 0. 78, P < 0. 01), while HOMA-IR had no significant relation with HDL-C ( P > 0.05).CONCLUSION: There is a close correlation between lipid metabolic disorder and insulin resistance in the high-risk diabetic group.
10.The Application of Correspondence Analysis toFactorial Experiment for Analysis of Cephalosporinwith Reversed Phase High Performance Liquid chromatography
Shiqian ZHU ; Kunyi NI ; Zhenjie WU ; Ze LI ; Qiguo ZHANG
Chinese Journal of Analytical Chemistry 2001;29(4):457-460
Correspondence analysis was used for determining the important factors from the six factors influencing analysis of cephalosporin in reversed phase-high performance liquid chromatography (RP-HPLC). Experiments were planed by U7(76), then the synthetical correspondence between a series of optimizations and the factors was calculated. Result indicated that that proportion of organic solvent, pH and concentration of buffer were three most important factors. It showed that the correspondence analysis was a better method to determining the important factors in RP-HPLC