1.Methylation status of RIZ1 gene promoter in myelodysplastic syndrome.
Rui-Rong XU ; Li-Na XUAN ; Yan WANG
Chinese Journal of Hematology 2012;33(9):774-775
Adolescent
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Adult
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Aged
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Aged, 80 and over
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DNA Methylation
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DNA-Binding Proteins
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genetics
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Female
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Histone-Lysine N-Methyltransferase
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genetics
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Humans
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Male
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Middle Aged
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Myelodysplastic Syndromes
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genetics
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Nuclear Proteins
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genetics
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Transcription Factors
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genetics
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Young Adult
2.Development and implementation of comprehensive experimental training of clinical nursing
Na LI ; Xuan ZHOU ; Xiaoyan LIU ; Shukun JIANG ; Juguo CHEN
Chinese Journal of Medical Education Research 2017;16(8):815-818
In order to train higher vocational nursing talents with strong theoretical knowledge,practical ability and humanistic quality,Kunming health Career Academy specially has developed clinical nursing comprehensive training course.Focusing on the training of students' occupation ability and the employment orientation,this course introduces multi-station concept,design and arrange the site teaching content,uses rich teaching methods and practices scientific and strict assessment,and especially it puts forward and implements intensive training t of site teaching before the practice of nursing students.Finally,the graduation test scores between the students who studied the course and the previous students who didn't are compared and analyzed.The result shows that the teaching of this course has obviously improved the theory,skill level and humanistic quality of nursing students in our hospital.
3.The study of matrix metalloproteinase-9 and tumor necrosis factor-or in predicting chorioamnhionitis in patients with premature rupture of membranes
Jin-Na HUANG ; Yuan-Cheng LI ; Li-Xuan ZHEN ; Shi-Xian GUO ; Chang GAO ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(10):-
Objective To study the relation of matrix metalloproteinase-9 in serum and tumor necrosis fac- tor-?in serum and amniotic fluid in predicting ehorioamnhionitis in patients with premature rupture of membranes (PROM).Methods The levels of MMP-9 in serum and TNF-?in serum and amniotie fluid were measured by ra- dioimmunoassay and ELISA in 67 cases with premature rupture of membranes as study group and 40 cases normal full-termed pregnant women as controls group.Results(1)The levels of TNF-?in amniotie fluid and MMP-9 in serum in study group were significantly higher than those in controls group(P0.05).(2)In study group,the levels of MMP-9 of serum in0.05).Conclusions The levels of TNF-?in amniotic fluid and MMP-9 in serum were valuable clinical indices for identification of chorioamnionitis in patients with PROM.The levels of MMP-9 in serum also could assess the time of rupture of membranes and the degree of ehorioamnionitis.
4.Clinicopathologic analysis of ovarian gonadoblastoma.
Xiu-Li WU ; Lian XU ; Ying HE ; Na YU ; Wei-Wei WU ; Kai-Xuan YANG
Chinese Journal of Pathology 2009;38(6):418-419
Adolescent
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Alkaline Phosphatase
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metabolism
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Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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Bleomycin
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therapeutic use
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Cisplatin
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therapeutic use
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Diagnosis, Differential
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Dysgerminoma
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pathology
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Etoposide
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therapeutic use
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Female
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Gonadoblastoma
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drug therapy
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metabolism
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pathology
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surgery
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Humans
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Hysterectomy
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methods
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Inhibins
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metabolism
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Isoenzymes
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metabolism
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Ovarian Neoplasms
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drug therapy
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metabolism
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pathology
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surgery
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Young Adult
5.Effect of Weifuchun on inhibiting inflammation of Helicobacter pylori-infected GES-1 cells and NF-kappaB signaling pathway.
Xuan HUANG ; Bin LU ; Shuo ZHANG ; Li-Na MENG ; Bing-Bing CHEN ; Min ZHAO
Chinese Journal of Integrated Traditional and Western Medicine 2014;34(4):450-454
OBJECTIVETo study the effect of Weifuchun on inflammation of Helicobacter pylori (Hp)-infected gastric epithelial cells (GES-1) and its correlation with NF-kappaB signaling pathway.
METHODSHp standard home-made strain (CagA +, VacA +) NCTCI 1637 infected GES-1 cells were used. Weifuchun was used as intervention. Weifuchun of different concentrations (5,10, and 20 microg/mL) were screened by MTT assay. A blank group and the model group were set up. Then the growth inhibition rate of drugs on gastric epithelial GES-1 cells was detected with MTT assay. Cell cycle was detected using flow cytometry. The supernatant liquid was separated to detect the contents of IL-8 and IL-4 by ELISA.The protein expression level of NF-kappaB was detected by Western blot analysis.
RESULTSMTT assay indicated significantly inhibitory effect of Weifuchun on GES-1 cells [5% inhibiting concentration (IC5)] was 10 microg/ml in the Weifuchun group. After GES-1 and Hp were cultured together,the contents of IL-8 in the supernatant were more obviously higher in the model group than in the blank group (P < 0.05), and then gradually decreased. After treatment with different concentrations of Weifuchun, the levels of IL-8 in the supernatant were less when compared with the model group at 12, 24, 48, and 72 h (P < 0.05). The decrement was the most significant in the high dose Weifuchun group. The IL-4 level in the supernatant was obviously lower in the model group than in the blank group. It obviously increased in the high concentration Weifuchun group (P < 0.05). There was no statistical difference in the IL-4 level between middle, low concentration Weifuchun group and the blank group (P > 0.05). The protein expression of intranuclear P65 increased and that of IkBalpha decreased 60 min after Hp infection. But the protein expression of intranuclear P65 decreased and the protein expression of IkBalpha increased after intervention of Weifuchun.
CONCLUSIONSWeifuchun adjusted H. pylori induced IL-8 and IL-4 production by gastric epithelial cells through blocking NF-kappaB pathways. Its mechanisms might possibly lie in inhibiting p65 from entry into nucleus and the degradation of IkBalpha. Weifuchun was an effective drug for treatment of Hp correlated chronic gastritis.
Cell Line ; Drugs, Chinese Herbal ; pharmacology ; Epithelial Cells ; drug effects ; metabolism ; Helicobacter Infections ; metabolism ; Helicobacter pylori ; Humans ; I-kappa B Proteins ; metabolism ; Inflammation ; Interleukin-4 ; metabolism ; Interleukin-8 ; metabolism ; NF-KappaB Inhibitor alpha ; NF-kappa B ; metabolism ; Signal Transduction ; drug effects ; Transcription Factor RelA ; metabolism
6.Analysis of the reliability and validity about scientific research competence indicators system of hospitals in Shanghai
Yuhong NIU ; Chunlin JIN ; Hansheng DING ; Na LI ; Shanshi YANG ; Minyu RAO ; Xuan LUO
Chinese Journal of Medical Science Research Management 2015;28(5):382-386,390
Objective To analyze the reliability and validity of an established Research Hospital Competitiveness Evaluation System.Methods To evaluate the research competitiveness of 34 tertiary hospitals and their departments in Shanghai using above system.The reliability and validity of the indicator system were analyzed with the Cronbach's alpha coefficient, and confirmatory factor analysis based on the structural equation model.Results After adjusting for structure of the index system via item parceling, the CFI, MFI, RMSEA AND IFI were 1.000, 1.009, 0.000 and 1.028, the Cronbach's alpha of whole set indicators system was 0.768.Conclusions The results suggested that the measured reliability and validity are both in good degree, survey results can reflect the actual competence of evaluated hospitals.
7.The impact of therapeutic regimen on the depression in type 2 diabetes mellitus: A cross-sectional study
Bing WU ; Xuan CHEN ; Li YANG ; Fangyuan ZHOU ; Na CHEN ; Jing WU
Chinese Journal of Endocrinology and Metabolism 2015;31(4):346-348
To explore the depression in type 2 diabetic patients treated with insulin and compared to those treated with oral anti-diabetic drugs.283 type 2 diabetics were seclected randomly from outpatient and inpatient departments of endocrionology in Jiangsu Province Hospital of Traditional Chinese Medicine,with the self-designed questionnaire and Zung self-rating depression scale to conduct the survey.Comparisons between the two groups were carried out with t-test or x2 test for quantitative and qualitative data,respectively.Logistic regression were used for the analysis of the relationship between the therapeutic regimen and depression.Overall,43.1% of the type 2 diabetic subjects showed depressive symptoms in different degrees.Compared to the oral drug group,the insulin group showed a significantly higher prevalence of depressive symptoms (insulin group,53.5%,oral drug group,30.5%;P<0.01)and higher self-rating depression scale scores (insulin group,51.7 ± 12.4,oral drug group,44.8 ± 10.6;P<0.01).Moreover,after an adjustment for age,sex,body mass index,diabetic duration,complications,HbA1Cand so on,the insulin group showed a significantly higher frequency of depression (OR=4.218,95% CI 1.764-13.285,P=0.004),compared to the oral drug group.The data showed that insulin treatment is an independent risk factor to the presence of depressive symptoms in type 2 diabetics,and it is necessary to pay more attention to their psychological support.
8.Analysis of monitoring data on drinking tea type of endemic fluorosis from 2009 to 2018 in Inner Mongolia Autonomous Region
Zili CHANG ; Na CUI ; Xiaojuan YANG ; Zhenlin LI ; Bo CHEN ; Yijun LIU ; Xuan WANG ; Chengxiang ZHAO
Chinese Journal of Endemiology 2021;40(2):118-123
Objective:To understand the change trend of people's condition in tea drinking endemic fluorosis area of Inner Mongolia Autonomous Region, and to provide scientific basis for formulating prevention and control strategies.Methods:From 2009 to 2018, a cross-sectional survey was conducted in 30 villages of 6 banners (counties) of drinking tea type of endemic fluorosis of Inner Mongolia Autonomous Region. The tea drinking habits of residents were investigated. Ten families were selected from each monitoring village to collect brick tea samples, and the fluorine content in brick tea was detected for health risk assessment. In each monitoring village, one water sample was collected from each drinking water source. Fluoride ion selective electrode method was used to detect fluorine in tea and water. The prevalence of dental fluorosis in children aged 8-12 years old was examined and the X-ray examination and analysis were carried out for all the permanent residents aged 36-45 years and living in the local area for more than 5 years.Results:From 2009 to 2018, a total of 3 000 brick tea samples were collected. The geometric mean value of tea fluorine was 522.01 mg/kg, and the annual per capita consumption of brick tea by permanent residents was 2.52 kg. The average daily intake of fluorine in brick tea was 4.11 mg. According to the standard of "Total Fluoride Intake of Population" (WS/T 87-2016), there were 6 years of risk quotient > 1 in 10 years, while according to the World Health Organization (WHO) recommend standard, there were 5 years of risk quotient > 1. From 2012 to 2018, 210 water samples were detected, and the geometric mean value of fluorine in water was 0.90 mg/L, and the qualified rate was 84.76% (178/210). A total of 5 384 children aged 8-12 years old were examined and 1 562 dental fluorosis patients were detected. The total detection rate of dental fluorosis in children was 29.01% (1 562/5 384), and the dental fluorosis index was 0.62 (very mild). A total of 1 890 adults aged 36 to 45 years old were examined by X-ray. The detection rate of skeletal fluorosis was 18.10% (342/1 890). There were 77 patients with moderate and severe skeletal fluorosis.Conclusions:The brick tea fluorine content of residents in Inner Mongolia Autonomous Region is high, and there are health risks. The dental fluorosis of children aged 8-12 years old is very mild, and there are still moderate and severe skeletal fluorosis patients in adults. Therefore, it is necessary to strengthen the supervision of brick tea market and strengthen health education for residents.
9.Application of diversity and pre-employment professional training in the newly-employed nurses training in military hospitals
Modern Clinical Nursing 2017;16(11):42-45
Objective To explore the applied effect of diversity and professional pre-employment training in the training of newly-employed nurses in military hospitals. Method The diverse and professional pre-employment training methods were used in the training of 52 newly-employed nurses in our hospital on ideological and political knowledge,military quality,psychological knowledge, nursing theory and skills and rules and regulations. Results The excellence rate about pre-employment training theory was 88.5%(46/52). More than 88.7% of newly-employed nurses were satisfied with the organization of pre-employment training, courses, teaching modes and methods,examination methods and teaching skills.Conclusion The diverse and professional pre-employment training in the military hospital is remarkable,for it can improve hired the comprehensive quality of the newly-employed nurses.
10.Roles of full-length and truncated hepatitis B virus X protein and of interactions with the host-encoded damaged DNA binding protein 1 in HBV replication.
Xuan YANG ; Song HE ; Na LUO ; Li LUO ; Hao FAN ; Qian GONG
Chinese Journal of Hepatology 2013;21(6):446-451
OBJECTIVETo investigate the roles of the hepatitis B virus (HBV)-encoded X protein (HBx), including the full-length and truncated isoforms, and in conjunction with the host-encoded damaged DNA binding protein 1 (DDB1) in HBV replication.
METHODSRecominant expression plasmids carrying the wild-type HBV genome (pGEM-HBV1.2) or with deletion of the full-length HBx protein (pHBV-deltaX), or carrying the full-length HBx protein (pSI-X) or the HBx1to101 (pSI-X1to101) or HBx43to154 (pSI-X43to154) isoforms were constructed for transfection into HepG2 cells. The pcDNA6.2-GW/EmGFP-miR (DDB1-miRNA) vector was constructed for silencing of the DDB1 gene in co-transfected HepG2 cells. At 72 h after transfections, DDB1 silencing was confirmed by western blot analysis and real-time quantitive reverse transcription PCR, HBV DNA copies number was assessed by real time PCR, and levels of hepatitis B surface antigen (HbsAg) and hepatitis B e antigen (HbeAg) were determined by ELISA. Differences between groups was statistically analyzed by single-factor analysis of variance and the t-test.
RESULTSTransfection with pHBV-deltaX led to reductions in DDB1 mRNA (to 52.74% of that in the wild-type pGEM-HBV1.2 transfected cells), HBV replication (to 55.49%), HBsAg level (48.05%), and HBeAg level (46.22%). Co-transfection with pSI-X or pSI-X43to154, but not with pSI-X1to101, restored the pHBV-deltaX-induced reductions in DDB1 mRNA, HBV replication, HBsAg and HBeAg to wild-type levels. The quantity of DDB1 mRNA was approximately parallel with the quantity of HBV DNA copies in all the HepG2 transfection groups.
CONCLUSIONThe COOH-terminal amino acids of HBx are required for HBV replication in hepatocytes, possibly involving the host-encoded DDB1 protein.
DNA-Binding Proteins ; metabolism ; Hep G2 Cells ; Hepatitis B Surface Antigens ; metabolism ; Hepatitis B e Antigens ; metabolism ; Hepatitis B virus ; metabolism ; physiology ; Host-Pathogen Interactions ; Humans ; Protein Isoforms ; metabolism ; Trans-Activators ; metabolism ; Transfection ; Virus Replication