AIM:To observe the effects of cornus iridoid glycosides(CIG)on inflammatory reaction especially the inflammatory cytokines in the brain after traumatic brain injury,and to explore the possible mechanisms of its neuroprotective effect.METHODS:SD rats were intragastrically administered with different doses of CIG(30,60 and 120 mg?kg-1?d-1)for 7 d.The traumatic brain injury rat model was induced by improved Feeney's fall weight method,and the brains were taken out 24 h and 72 h after brain injury,respectively.The morphological changes were observed by HE staining in the cerebral cortex.The expressions of inflammatory cytokine tumor necrosis factor-?(TNF-?)and interleukin-1?(IL-1?)were detected by immunohistochemical method.The image processing and statistical analysis were used to measure the number and the area of immunoreactive cells.RESULTS:HE staining showed the pathological changes were serious in the cerebral cortex of model group,and compared with the model group,the pathological changes were obviously reduced in CIG group.The positive immunoreactive cells of TNF-? and IL-1? were mainly distributed around the foci of contusion,the expressions of TNF-? and IL-1? in the model group were significantly higher than those in sham operated group,and the high expressions were sustained from 24 h to 72 h after brain injury.Compared with the model group,the levels of TNF-? and IL-1? in the brain of CIG treatment groups were obviously decreased in a dose-dependent manner and the inhibitory effects of TNF-? and IL-1? were more significant at 72 h after brain injury.CONCLUSION:CIG may have neuroprotective effect on traumatic brain injury through inhibiting the expression of inflammatory cytokines and reducing the inflammatory reaction.

Good animal models of traumatic brain injury have a great significance in the pathogenesis research and treatment study. This paper reveiwed development in animal models of traumatic brain injury, including preparation Methods , application range, characteristics and shortcomings of each model.

The rupture of vulnerable carotid atherosclemtic plaques and distal embolization of atherornatous debris are the most important pathogenesis of atherosclerotic stroke.The serolog- ic(and plasma)markers associated with vulnerable plaques will change significantly in this process,and show their different meanings.The studies in this field are in its infancy and still need to be further validated,This article reviews the advances in research on the correlation of these biomakers with vulnerable plaque and ischemic cerebrovascular disease,and briefly intro- duces the clinical application prospect of some of the biomakers.

AIM:To evaluate the efficacy and safety of Sub-tenon's anesthesia for compound trabeculectomy with high intraocular pressure.
METHODS: Forty - six eyes ( 46 cases ) of primary glaucoma received compound trabeculectomy under Sub-tenon's anesthesia, whose preoperative intraocular pressure were higher than normal after 24 to 48h of combined medication. Both efficacy and complication of the anesthesia were studied.
RESULTS: One minute after anesthetic injection, all cases were able to achieve the effect of analgesia and eye brake. During the operation, 0 level of anesthesia effect included 35 eyes ( 76%) , 1 level of anesthesia effect included 10 eyes ( 22%) , 2 level of anesthesia effect included 1 eye(2%). Only 1 case of these patients needed to add the surface anesthetic once, and other cases were successfully operatedunder Sub-tenon's anesthesia. The total effective rate was 98%. No anesthesia complications occurred in all cases.
CONCLUSION: Sub - Tenon's anesthesia is safe, effective, simple and quick for compound trabeculectomy with high intraocular pressure.

Objective To investigate effect of compound mylabris capsules combined with neoadjuvant on efficacy and expression of Ki67,ER,PR in patients with HER2-negative breast carcinoma.Methods Eighty-one HER2 negative-breast cancer patients from the hospital were randomly divided into treatment group(41cases) and control group(40 cases) by random number table method.Control group was treated with three weeks of neoadjuvant chemotherapy,firstly given with cyclophosphamide(CTX)600 mg/m2 +adriamycin amycin(Ad)60 mg/m2,and then next course with docetaxel(DOC) 100 mg/m2, intravenous drip for 1 h,one period per three weeks,one times per week,and for 6 periods.Patients in treatment group were additionally given with compound mylabris capsules from one day before chemotherapy,three particles per time,two times per day,seven times per period,and for another one week after chemotherapy.Score of traditional Chinese medicine(TCM) symptoms,clinical efficacy,and levels of hemorheology indexs were compared.Serum levels of Ki67,ER,and PR were detected between both groups.Results Total efficacy of treatment group was recently 56.10%,which was superior to control group 30.00%(P<0.05).AT the ending of chemotherapy and 1 weeks after chemotherapy,TCM score, plasma viscosity,and high, middle and low shear of whole blood were obviously lower than control group(P<0.05).Serum level of Ki67 in treatment group was lower, while ER and PR were higher than control group at the ending of chemotherapy and 1 weeks after chemotherapy with statistically significant difference ( P<0.05).Conclusion Compound mylabris capsules combined with neoadjuvant chemotherapy in treating breast carcinoma with HER2 negative can decrease TCM symptoms, improve blood viscosity and clinical efficacy,and inhibit expression of Ki67 and up-regualte ER and PR levels.

Objective To investigate the function of mesothelin by analyzing the location,the expressions and the adhesive ability of mesothelin and CA125 in human epithelial ovarian cancer.Methods Mesothelin and CA125 protein expressions and locations in human epithelial ovarian cancer tissues and cell lines were determined by indirect immunofluorescence double maker and Western blot.Cell adhesive ability was detected by EDTA-induced cell detachment.Results Mesothelin and CA125 were specifically located in cytomembrane of human epithelial ovarian cancer cells.Expressions of mesothelin in the SKOV-3 and 3AO cells were weaker than those in the OVCAR-3.SKOV-3 and 3AO cells adhered less effectively to plastic substance and matrigel than OVCAR-3(P

Objective:To construct a recombinant lentivirus plasmid of RNA interference targeting (MSLN) gene and to observe its effect on MSLN expression in human ovarian cancer cell line OVCAR-3 and its effect on cell proliferation. Methods: According to the Genbank information of MSLN, four RNA interfering sequences and a negative sequence were designed and inserted into plasmid pRNAT-U6.2/Lenti and 5 kinds of plasmids were packaged: LV-MSLN-negative,LV-MSLN-shRNA1, LV-MSLN-shRNA2, LV-MSLN-shRNA3, and LV-MSLN-shRNA4; and they were used to transfect OVCAR-3 cells. Western blotting and indirect immunofluorescence were then used to investigate the interfering efficiency. The plasmid with high interfering efficiency was packaged. The cell proliferation test and clone-forming test was used to assess the changes in cell proliferation. Results: DNA sequencing showed that the sequences of 5 recombinant lentivirus plasmids were correct. Lentivirus packaging was successfully done. Western blotting analysis confirmed that LV-MSLN-shRNA4 had the highest interfering efficiency (90%). MSLN specifically bound to cytomembrane of OVCAR-3 cells. Expression of MSLN in the interfered cells (OVC-shRNA) was weaker than that in the control cells (OVC-neg,OVC). OVC-shRNA cells([11.2?1.3]?105) grew slowly compared to OVC-neg cells([20.5?2.5]?105) and OVC cells([21.9?2.3]?105) (P

Objective To find out the relationship between fetal growth restriction (FGR) and transforming growth factor -β1 (TGF-β1). Methods The levels of TGF-β1 in maternal serum and placental tissue of FGR were detected with using ELISA and immunohistochemistry technique, and it was compared with those of normal term pregnancy. Results The levels of TGF-β1 in maternal serum of FGR group were significantly higher than those of normal term pregnancy (76. 5 ± 33. 4 VS 47.6 ± 24. 2, t' = 4. 65, P ＜0. 05). As for the intensity of the immunohistochemistry signal, TGF-β1 in syncytiotrophoblastic cells of FGR was markedly higher than that of control group (81.82% vs 5.83%, P ＜0. 01). Conclusions The levels of TGF-β1 are closely related with FGR.

Adult ; Churg-Strauss Syndrome ; complications ; Humans ; Male ; Myocardial Infarction ; complications

BACKGROUND:Some studies show that basic fibroblast growth factor(bFGF)strongly expresses dudng the process of embryonic stem cells differentiation into hematopoietic stem cell.yolk sac blooding.and fetal liver hematopoiesis.OBJECTIVE:To study the regulation of bFGF on the blast-colony-forming cell(BL-CFC)by adding bFGF in the medium of embryoid body generation.METHODS:The third to fifth generations of the pdmaw mouse embryonic fibroblasts were recovered,and then incubated with the DMEM medium containing mitomvcin C for 2.5 hours in order to lose the proliferative capacity.Then cells were suspended into single cell by trypsinization and inoculated in the gelatin-coated bottle at the density of 10×104/cm2.After culturing for 24 hours,mouse embryonic stem cells(mESC)of D3 were recovered and placed on the feeder layer cells.According to the composition of medium in embryoid body generation.mESCs were divided into two groups:group A:standard medium+VEGF+SCF;group B:standard medium+VEGF+SCF+bFGF.Each group was cultured for 3 days and 6 days respectively,and the cloning number of BL-CFC was quantified,as well as Flk-1+ expression was observed by immunofluorescence staining.Positive number and average absorbance were analyzed using IMAGE-PRO PLUS imaging analysis system.RESULTS AND CONCLUSION:Adding bFGF in the course of embryoid body growth could significantly increase the number of BL-CFC(P＜0.01).and the positive results of Flk-1 and the average absorbance were also increased significantly(P＜0.01).bFGF effectively promoted embryoid body amplification and proliferation of BL-CFC.