2.Application of multivariate statistical analysis and thinking in quality control of Chinese medicine.
Na LIU ; Jun LI ; Bao-Guo LI
China Journal of Chinese Materia Medica 2014;39(21):4268-4271
The study of quality control of Chinese medicine has always been the hot and the difficulty spot of the development of traditional Chinese medicine (TCM), which is also one of the key problems restricting the modernization and internationalization of Chinese medicine. Multivariate statistical analysis is an analytical method which is suitable for the analysis of characteristics of TCM. It has been used widely in the study of quality control of TCM. Multivariate Statistical analysis was used for multivariate indicators and variables that appeared in the study of quality control and had certain correlation between each other, to find out the hidden law or the relationship between the data can be found,.which could apply to serve the decision-making and realize the effective quality evaluation of TCM. In this paper, the application of multivariate statistical analysis in the quality control of Chinese medicine was summarized, which could provided the basis for its further study.
Medicine, Chinese Traditional
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standards
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Multivariate Analysis
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Quality Control
3.Clinical study with prostat for treatment of chronic nonbacterial prostatitis
Ningchen LI ; Yanqun NA ; Hongqian GUO
Chinese Journal of Urology 2001;0(09):-
Objective To evaluate the efficacy and safety of prostat in the treatment of chronic nonbacterial prostatitis (CNP). Methods A multicentral open clinical trial was conducted.A total of 115 CNP patients were enrolled in the trial.For each patient 1 tablet of prostat (70 mg P5+4 mg EA10) was orally administered twice a day. Results After 4- and 8- week therapy,NIH-CPSI overall scores were averagely decreased 7.1 (29.5%) and 11.9 (49.4%),respectively.Symptom rating scores were averagely decreased 5.0 (32.1%) and 8.2 (52.5%),respectively.Quality of life scores were averagely decreased 2.1 (24.6%) and 3.7 (43.9%),respectively.WBC counts in prostate massage fluid were averagely decreased 8.8/HP (39.3%) and 13.9/HP (61.9%),respectively.The total effective rate was 82.1% after 8 week therapy.No adverse reaction associated with medication was observed. Conclusions Prostat is effective and safe in relieving the subjective symptoms and improving the objective evaluation in the treatment of CNP patients.
4.Clinical and pathological features of limb-girdle muscular dystrophy type 2B
Hua GUO ; Na LI ; Dongcai YUAN
Journal of Clinical Neurology 2001;0(05):-
Objective To investigate the clinical and pathological features of limb-girdle muscular dystrophy type 2B(LGMD2B).Methods The clinical data and the pathological result of skeletal muscle in five patients with LGMD2B were analyzed retrospectively.Results Five patients presented muscle atrophy and weakness(four limbs in three patients and both lower limbs in two patients),and the chronic onset,and the progressive deterioration.The pathological examination showed that the muscle fibers degenerating,necrotic and regenerating in different extents were observed,and the infiltration of inflammatory cells were appeared on all of cases.Immunohistochemical stains showed that the expression of anti-dysferlin monoclonal antibody was negative,anti-Dystrophy,Sarcoglycan and dystroglycan monoclonal antibodies were positive normaly,anti-CD8+T cell monoclonal antibody was negative,and anti-MHC-1 monoclonal antibody was up-regulated in five patients.Conclusions The clinical characters of LGMD2B are chronic onset,progressive muscle atrophy and weakness in four limbs or both lower limbs.The pathological characters of LGMD2B are similar to the changes of polymyositis.
5.Clinical Application of Endometrial Scratching in Patients with Repeated Implantation Failure
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong 2015;(5):578-581
Objective To explore the clinical application of endometrial scratching in patients with repeated implantation failure(RIF).Methods A total of 189 women with RIF who planned to undergo in vitro fertilization and embryo transfer(IVF‐ET)once again at the Reproductive Medicine Center of Tongji Hospital ,Huazhong University of Science and Technology ,be‐tween October 2013 and August 2014 ,were prospectively examined.These women were infertile due to tubal factors ,with their male partners having normal semen measurements.According to the case numbers ,the 189 patients were randomly divided into experimental group(n=96)and control group(n=93).In the experimental group ,endometrial scratching was conducted within 6 h of one menstrual cycle one month before oocyte retrieval ,while in control group ,no endometrial scratching was performed at that time.The outcomes of pregnancy were compared between the two groups after embryo transplantation.Results There was no statistical difference between the two groups in the patient age ,infertility duration ,body mass index(BMI) ,basal levels of follicle‐stimulating hormone(FSH) ,luteinizing hormone(LH) ,and progesterone(P) ,gonadotropin‐releasing hormone(Gn‐RH) injection days and doses ,the number of retrieved oocytes ,the number of transferred embryos ,the endometrial thickness and lev‐els of estradiol(E2 )and P on the day of HCG injection(P>0.05).The embryo implantation rate ,the clinical pregnancy rate and the ongoing pregnancy rate in the experimental group were much higher than those in the control group ,with statistically signif‐icant difference noted(P<0.01).Conclusion The endometrial scratching can improve the endometrial receptivity and enhance the clinical pregnancy rate of RIF patients.
6.Progress in clinicopathologic study on uterine smooth muscle tumor of uncertain malignant potential.
Chinese Journal of Pathology 2011;40(8):573-576
Cyclin-Dependent Kinase Inhibitor p16
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metabolism
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Female
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Genes, p16
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Genes, p53
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Humans
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Hysterectomy
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methods
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Ki-67 Antigen
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metabolism
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Leiomyoma
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genetics
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metabolism
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pathology
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surgery
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Leiomyosarcoma
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genetics
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metabolism
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pathology
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surgery
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Prognosis
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Smooth Muscle Tumor
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genetics
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metabolism
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pathology
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surgery
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Tumor Suppressor Protein p53
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metabolism
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Uterine Neoplasms
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genetics
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metabolism
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pathology
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surgery
7.Study of gonadal hormone drugs in blocking filovirus entry of cells in vitro.
Li-li WANG ; Qing CHEN ; Li-na ZHOU ; Ying GUO
Acta Pharmaceutica Sinica 2015;50(12):1545-1550
This study was designed to discover filovirus entry inhibitors in a drug library of commercial medicines. One thousand and six hundred drugs were screened using the ZEBOV-GP/HIV model, a pseudovirus formed by an HIV-core packed with the Zaire Ebola virus glycoprotein. We identified 12 gonadal hormone drugs with inhibitory activities in ZEBOV-GP/HIV entry at final concentration of 10 μmol x L(-1). Among them, three drugs exhibited strong activities with IC50 < 1 μmol x L(-1), such as toremifene citrate (IC50: 0.19 ± 0.02 μmol x L(-1)), tamoxifen citrate (IC50: 0.32 ± 0.01 μmol x L(-1)) and clomiphene citrate (IC50: 0.53 ± 0.02 μmol x L(-1)); seven drugs had moderate activities with IC50 between 1 and 10 μmol x L(-1), such as estradiol benzoate (IC50: 1.83 ± 5.69 μmol x L(-1)), raloxifene hydrochloride (IC50: 3.48 ± 0.07 μmol x L(-1)), equilin (IC50: 4.00 ± 9.94 μmol x L(-1)), estradiol (IC50: 5.26 ± 9.92 μmol x L(-1)), quinestrol (IC50: 6.36?5.37 gmol-L1), estrone (IC50: 6.87 ± 0.03 μmol-L1) and finasteride (IC50: 9.94 ± 0.45 μmol x L(-1)); two drugs, hexestrol (IC50: 14.20 ± 0.55 μmol x L(-1)) and chlormadinone acetate (IC50: 24.60 ± 0.36 μmol x L(-1)), had weak activities against ZEBOV. Further, toremifene citrate, tamoxifen citrate, clomiphene citrate, raloxifene hydrochloride and quinestrol could block both pseudovirus type Sudan ebola virus (SEBOV-GP/HIV) and Marburg virus (MARV-GP/HIV) entries.
Antiviral Agents
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pharmacology
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Drug Evaluation, Preclinical
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Ebolavirus
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drug effects
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physiology
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Hemorrhagic Fever, Ebola
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Humans
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Marburgvirus
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drug effects
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physiology
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Selective Estrogen Receptor Modulators
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pharmacology
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Virus Internalization
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drug effects
8.Ovarian endometrial adneocarcinoma with choriocarcinomatous differentiation: a case report.
Li MA ; Li-na GUO ; Tong-hua LIU
Chinese Journal of Pathology 2005;34(7):442-443
Adenocarcinoma
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metabolism
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pathology
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surgery
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Adult
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Choriocarcinoma
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Endometrial Neoplasms
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metabolism
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pathology
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surgery
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Female
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Humans
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Keratin-7
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metabolism
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Mucin-1
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metabolism
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Ovarian Neoplasms
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metabolism
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pathology
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surgery
9.Advances in Sertoli-Leydig cell tumour of the ovary.
Jing-li SHI ; Li-na GUO ; Jing-he LANG
Chinese Journal of Pathology 2008;37(9):631-633
Female
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Genes, p53
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immunology
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Humans
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Ovarian Neoplasms
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genetics
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pathology
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Ovary
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pathology
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Proto-Oncogene Proteins
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immunology
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metabolism
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Proto-Oncogene Proteins p21(ras)
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Proto-Oncogenes
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immunology
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Sertoli-Leydig Cell Tumor
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genetics
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pathology
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ras Proteins
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immunology
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metabolism
10.Cementoblastoma: report of a case.
Wen-ze WANG ; Ding-rong ZHONG ; Li-na GUO
Chinese Journal of Pathology 2005;34(4):253-253
Adult
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Cementoma
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pathology
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surgery
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Diagnosis, Differential
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Humans
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Male
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Mandibular Neoplasms
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pathology
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surgery
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Tooth Root
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pathology
;
surgery