Objective To investigate the effect of Doxycycline on gastric cancer cell line BGC-823 proliferation and Notch1, Matrix metalloproteinase-9(MMP-9)expression in order to shine a light on new anticancer drugs. Methods Final concentration of 0, 5, 10, 20, 40 mg/L doxycycline were added into human gastric cancer cell line BGC-823. Cell pro-liferation was detected by MTT;Cell cycle distribution was assessed by flow cytometry;Notch1, MMP-9 protein expression was revealed by Immunoblot. Results Doxycycline can inhibit proliferation of human gastric cancer cells line BGC-823, and its effect is dose and time-dependent(P＜0.01). Doxycycline alters distribution of gastric cancer cell line BGC-823. With increasing drug concentration, the proportion of cells in S phase dropped(P＜0.01). Notch1 expression rose and MMP-9 expression decreased(P＜0.01). Conclusion Doxycyclinecan inhibited gastric cancer cell line BGC-823 prolif-eration and up-regulating Notch1 might be one mechanisms.

Forkhead box Q1 (FoxQ1) is a transcription factor protein with the function of cell differentiation regulation.Recently,increasing evidence has demonstrated that FoxQ1 is significantly associated with pathogenesis of digestive system tumors.This article systematically reviewed the expression and the role of FoxQ1 in the pathogenesis of digestive system tumors.Potential therapeutic target of FoxQ1 was discussed as well.

Objective:To study the public health Emergency Operations Centers (EOCs)in the US, the European Union, the UK and Australia, and summarize the good practice for the improvement of National Health Emergency Response Command Center in Chinese National Health and Family Planning Commission.Methods:Literature review was conducted to explore the EOCs of selected countries.Results:The study focused on EOC function, organizational structure, human resources and information management. The selected EOCs had the basic EOC functions of coordinating and commanding as well as the public health related functions such as monitoring the situation, risk assessment, and epidemiological briefings. The organizational structures of the EOCs were standardized, scalable and flexible. Incident Command System was the widely applied organizational structure with a strong preference. The EOCs were managed by a unit of emergency management during routine time and surge staff were engaged upon emergencies. The selected EOCs had clear information management framework including information collection, assessment and dissemination.Conclusions:The performance of National Health Emergency Response Command Center can be improved by learning from the good practice of the selected EOCs, including setting clear functions, standardizing the organizational structure, enhancing the human resource capacity and strengthening information management.

Animals ; Diabetes Mellitus, Experimental ; metabolism ; Diaphragm ; drug effects ; Lentinan ; pharmacology ; Male ; Mitochondria ; drug effects ; metabolism ; Nitric Oxide Synthase ; metabolism ; Rats ; Rats, Sprague-Dawley ; Succinate Dehydrogenase ; metabolism ; Superoxide Dismutase ; metabolism

Animals ; Cochlea ; metabolism ; Environmental Exposure ; adverse effects ; Hearing Loss, Noise-Induced ; metabolism ; Humans ; Noise ; adverse effects ; Oxidative Stress ; physiology ; Reactive Oxygen Species ; metabolism

Objective To investigate the relation between the expression of HSP70 and p57kip2 in C, CT and Enneking surgical classifi-cation by detecting expression of HSP70 and p57kip2. Methods 30 cases of GCT were collected, including surgical operation specimens and 13 cases of osteochondroma were taken as control group. The expression of HSP70 and p57kip2 were detected, and the relation between the ex-pression of HSP70 and p57kip2 and Enneking surgical classification and prognosis of GCT were analyzed. Results With the increase of tumor Enneking surgical grades, the positive rate of HSP70 was increased and the negative rate of p57kip2 was decreased. Expression rates of HSP70 were 22. 2%, 88.9% ,100% in grades of 1～3 and expression rates of p57kip2 were 88. 9%, 88.9%, 33.3% in grades of 1～3. There was a negative correlation between HSPTo and p.57kip2 expression. Conclusions HSP70 and p57kip2 may play a role in the genesis, and develop-ment of giant cell tumor of bone, and it can be helpful for surgical classification and prognesis of GCT.

Objective:To investigate the expression of transforming growth factor-?1 (TGF-?1) in mandibular condylar chondrocytes after the condylar cartilage was stimulated by compressive stress.Methods:20 condylar cartilage samples were obtained from 10 new borne SD rats and cultured for 24 h.Then,10 samples were treated by a static compressive stress with a magnitude of 10 kPa for 1 h.Another 10 samples without stress treatment were used as the controls. At end of stress stimulation, the expression of TGF-?1 in the samples of both groups was examined by immunohistochemistry and image pattern analysis.Results:The grayscale values of TGF-?1 in perichondrium lay of stress treated and control samples were 159.21?19.84 and 174.20?14.47(P

Objective To observe the effect of electroacupuncture(EA) on long-term potentiation(LTP),calmodulin(CaM) and calmodulin protein kinase Ⅱ(CaMPKⅡ) mRNA expression in vascular dementia rats.Methods Seventy SD rats,half in male and aged 2 months,were randomized into 5 groups: normal group(N =14),pseudo-operation group(N = 14),EA group(N = 12),western medicine group(N = 12),and model group(N =12).EA group were given acup uncture on Baihui(GV20),Dazhui(GV14) and Shenshu(BL23),and western medicine group were given nimoldipine 12 mg.kg-1.d-1.The normal group,model group and pseudo-operation group were fed routinely without any medication.Escape latency and frequency of rats crossing the quadrant of the platform in Morris water maze,in-vivo hippocampal LTP,and neuronal CaM and CaMPKⅡ mRNA expression were observed.Results Compared with the model group,escape latency was shortened,frequency of rats crossing the original and left quadrants of the platform rised,LTP amplitude and duration increased,and neuronal CaM and CaMPKⅡ mRNA expression enhanced in EA group and western medicine group(P

Objective To analysis the genetic context of armA gene identified in different species .Methods BLASTN searches were used to analysis sequences harboring armA submitted to the GenBank database .Results armA gene was mostly found down-stream of ISCR1 element and was most commonly detected in Enterobacter spp ..Its flanking sequences were highly conserved in Enterobacter spp ..Conclusion The genetic context of armA gene reported in different species scattered in different geometrical lo-cations share some common characteristics .

Objective To observe the real-time efficacy of balance acupuncture in treating primary hypertension. Method Totally 160 patients with primary hypertension were randomized into a treatment group and a control group, 80 in each group. The treatment group was intervened by balance acupuncture, while the control group was by oral administration of Captopril. The blood pressure and symptom score were observed before and after intervention. Result There were no significant differences in comparing the total effective rate at different time points between the two groups (P>0.05). There was a significant difference in comparing the headache score in male patients at 30 min after treatment between the two groups (P<0.05). Conclusion Balance acupuncture is an effective method in treating primary hypertension, and can release headache of the patients.