Forkhead box Q1 (FoxQ1) is a transcription factor protein with the function of cell differentiation regulation.Recently,increasing evidence has demonstrated that FoxQ1 is significantly associated with pathogenesis of digestive system tumors.This article systematically reviewed the expression and the role of FoxQ1 in the pathogenesis of digestive system tumors.Potential therapeutic target of FoxQ1 was discussed as well.

Objective To investigate the effect of Doxycycline on gastric cancer cell line BGC-823 proliferation and Notch1, Matrix metalloproteinase-9(MMP-9)expression in order to shine a light on new anticancer drugs. Methods Final concentration of 0, 5, 10, 20, 40 mg/L doxycycline were added into human gastric cancer cell line BGC-823. Cell pro-liferation was detected by MTT;Cell cycle distribution was assessed by flow cytometry;Notch1, MMP-9 protein expression was revealed by Immunoblot. Results Doxycycline can inhibit proliferation of human gastric cancer cells line BGC-823, and its effect is dose and time-dependent(P＜0.01). Doxycycline alters distribution of gastric cancer cell line BGC-823. With increasing drug concentration, the proportion of cells in S phase dropped(P＜0.01). Notch1 expression rose and MMP-9 expression decreased(P＜0.01). Conclusion Doxycyclinecan inhibited gastric cancer cell line BGC-823 prolif-eration and up-regulating Notch1 might be one mechanisms.

Objective:To study the public health Emergency Operations Centers (EOCs)in the US, the European Union, the UK and Australia, and summarize the good practice for the improvement of National Health Emergency Response Command Center in Chinese National Health and Family Planning Commission.Methods:Literature review was conducted to explore the EOCs of selected countries.Results:The study focused on EOC function, organizational structure, human resources and information management. The selected EOCs had the basic EOC functions of coordinating and commanding as well as the public health related functions such as monitoring the situation, risk assessment, and epidemiological briefings. The organizational structures of the EOCs were standardized, scalable and flexible. Incident Command System was the widely applied organizational structure with a strong preference. The EOCs were managed by a unit of emergency management during routine time and surge staff were engaged upon emergencies. The selected EOCs had clear information management framework including information collection, assessment and dissemination.Conclusions:The performance of National Health Emergency Response Command Center can be improved by learning from the good practice of the selected EOCs, including setting clear functions, standardizing the organizational structure, enhancing the human resource capacity and strengthening information management.

Animals ; Diabetes Mellitus, Experimental ; metabolism ; Diaphragm ; drug effects ; Lentinan ; pharmacology ; Male ; Mitochondria ; drug effects ; metabolism ; Nitric Oxide Synthase ; metabolism ; Rats ; Rats, Sprague-Dawley ; Succinate Dehydrogenase ; metabolism ; Superoxide Dismutase ; metabolism

Objective To investigate the relation between the expression of HSP70 and p57kip2 in C, CT and Enneking surgical classifi-cation by detecting expression of HSP70 and p57kip2. Methods 30 cases of GCT were collected, including surgical operation specimens and 13 cases of osteochondroma were taken as control group. The expression of HSP70 and p57kip2 were detected, and the relation between the ex-pression of HSP70 and p57kip2 and Enneking surgical classification and prognosis of GCT were analyzed. Results With the increase of tumor Enneking surgical grades, the positive rate of HSP70 was increased and the negative rate of p57kip2 was decreased. Expression rates of HSP70 were 22. 2%, 88.9% ,100% in grades of 1～3 and expression rates of p57kip2 were 88. 9%, 88.9%, 33.3% in grades of 1～3. There was a negative correlation between HSPTo and p.57kip2 expression. Conclusions HSP70 and p57kip2 may play a role in the genesis, and develop-ment of giant cell tumor of bone, and it can be helpful for surgical classification and prognesis of GCT.

Animals ; Cochlea ; metabolism ; Environmental Exposure ; adverse effects ; Hearing Loss, Noise-Induced ; metabolism ; Humans ; Noise ; adverse effects ; Oxidative Stress ; physiology ; Reactive Oxygen Species ; metabolism

Background Wearing of soft contact lens,as one of the important methods to correct ametropia,has been widely applied at present.The clinically deleterious effects of soft contact lens have been reported,but researches about influence of apoptosis of corneal epithelial cells are scant.Objective This study was to detect and compare the ratio of apoptosis of corneal epithelial cells between the patients with contact lens wearing and without contact lens wearing,and explore the influence of soft contact lens wearing for long term on growth of corneal epithelial cells after injury.Methods A retrospective nest case-controlled study was designed.Forty eyes of 20 myopic patients with wearing of soft contact lens for ≥5 years and 40 eyes of 20 myopic patients without wearing of soft contact lens were included in Affiliated Hospital of Guiyang Medical College.All the patients received the off-flap epipolis laser in situ keratomileusis (Epi-LASIK) from 2010 October to 2011 June.The corneal flaps were collected during the surgery.The apoptosis of corneal epithelial cells was detected by flow cytometry(FCM),and the difference of apoptotic proportion between the two groups was compared using independent sample t test.Results The FCM scatter plot showed that compared to the without contact lens wearing group,the early stage of apoptotic cells with high staining annexin V and low staining proaium iodide (PI) were much more,and survival cells with low staining annexin V and PI were less,and necrotic and late stage of apoptotic cells with high staining of annexin V/PI were more in the with contact lens wearing group.The early stage of apoptosis proportion of corneal epithelial cells was (11.23 ± 5.31)％ in the with contact lens wearing group,and that in the without contact lens wearing group was (7.31± 5.43)％,showing a significant difference between them (t=2.754,P=0.008).Conclusions A long term wearing of contact lens induce more apoptosis of corneal epithelial cells,which can result in a disorder of structure and function of ocular surface during the wound healing stage.

[Objective] To observe the influence of Red Sage and Propranolol on portal hypertension rats' pressure,liver fibrosis and gastric-intestinal hormone.[Method] At 4w after making models,rats take the said medicines,then measure their portal pressure with biological signal Medlab-Ug4Cs collection and treatment system;after decapitation,measure the indexes with radio-immune method.[Result] In model group(1),the portal pressure is much more increased than normal one(2),that in Red Sage treatment(3) and Propranolol(4) groups is much lowered,so are gastrin and glucagon;compared to group 1,the gastrin,motilin and glucagon in group 3 are much decreased,so are compared to group 4.[Conclusion] Red Sage can effectively reduce portal pressure,liver fibrosis index and regulate gastro-intestinal hormone,with cure effect better than propranolol.

Objective:To investigate the expression of transforming growth factor-?1 (TGF-?1) in mandibular condylar chondrocytes after the condylar cartilage was stimulated by compressive stress.Methods:20 condylar cartilage samples were obtained from 10 new borne SD rats and cultured for 24 h.Then,10 samples were treated by a static compressive stress with a magnitude of 10 kPa for 1 h.Another 10 samples without stress treatment were used as the controls. At end of stress stimulation, the expression of TGF-?1 in the samples of both groups was examined by immunohistochemistry and image pattern analysis.Results:The grayscale values of TGF-?1 in perichondrium lay of stress treated and control samples were 159.21?19.84 and 174.20?14.47(P

Objective To observe the effect of electroacupuncture(EA) on long-term potentiation(LTP),calmodulin(CaM) and calmodulin protein kinase Ⅱ(CaMPKⅡ) mRNA expression in vascular dementia rats.Methods Seventy SD rats,half in male and aged 2 months,were randomized into 5 groups: normal group(N =14),pseudo-operation group(N = 14),EA group(N = 12),western medicine group(N = 12),and model group(N =12).EA group were given acup uncture on Baihui(GV20),Dazhui(GV14) and Shenshu(BL23),and western medicine group were given nimoldipine 12 mg.kg-1.d-1.The normal group,model group and pseudo-operation group were fed routinely without any medication.Escape latency and frequency of rats crossing the quadrant of the platform in Morris water maze,in-vivo hippocampal LTP,and neuronal CaM and CaMPKⅡ mRNA expression were observed.Results Compared with the model group,escape latency was shortened,frequency of rats crossing the original and left quadrants of the platform rised,LTP amplitude and duration increased,and neuronal CaM and CaMPKⅡ mRNA expression enhanced in EA group and western medicine group(P