There are not yet uniform for the recognition and morphological description of Russell bodies and Mott cells.Some difficulties for the learner in clinical diagnosis were encountered.The characteristics of name and morphology of Russell bodies and Mott cells were described in this paper combined with many years of experience in clinical practice according to new point of view of (2008) World Health Organization classification of tumours of haematopoietic and lymphoid tissues and European Leukemia Net Morphology Faculty.

Based on the current situation of cell morphology of Chediak-Higashi syndrome (CHS) and the reasons for missed diagnosis or misdiagnosis of CHS,the microscopic examination on peripheral blood smear should be emphasized. Some suggestions was proposed for improving staff′s understanding of CHS,unifying the standard of morphological diagnosis of CHS and performing further researches.

Three neo-clerodane were isolated from the aerial parts of Salvia farinacea Benth., and were purified by various technologies, including silica gel, ODS, sephadex LH-20, and their structures were identified by modern spectroscopy techniques as 2β-hydroxy-7,8-dehydrobacchotricuneatin A (1), dugesin C (2), and tonalensin (3). Compound 1 was a new diterpenoid, and compounds 2 and 3 were isolated from this plant for the first time.

Objective To explore the imaging characteristics of seminomas and nonseminomatous germ cell tumors (NSGCT) and its pathological foundation. Methods CT and MR imaging manifestations in 25 cases of testicular germ cell tumors proved by pathological examinations were analyzed retrospectively. All tumors were divided into seminomas group (12 cases) and NSGCT group (13 cases). In the seminomas group, 5 cases were examined by CT and 4 of those also had contrast enhanced CT. Seven cases had MRI and 4 of those had dynamic enhanced MRI. In the NSGCT group, 5 were examined by plain CT in which two were by contrast enhanced CT, eight were by MRI in which 4 were by dynamic enhanced MRI. CT or MRI characteristics (morphology, density or intensity, enhancement) in both groups were analyzed by Fisher test. Results Histological examination revealed 25 intratesticular lesions. In 12 seminomas, 10 showed a nodular/lobulated shape, 5 showed a mixed density or intensity. In 13 NSGCT, only one lesion showed a lobular shape, 11 showed a mixed density or intensity. Seven seminomas showed a low signal on T2WI on MRI while only two NSGCT showed this sign. In four lesions underwent dynamic MRI scanning, 3 showed fibrous septum enhancement while no lesions in NSGCT showed this sign. The occurrence rate of the above imaging characteristics in both group was significantly different (P<0.05). Conclusion Seminomas and NSGCT may have their own CT and MRI characteristics, which may be of great value for differential diagnosis .

Blood Cell Count ; Child ; Fever ; Histiocytosis, Non-Langerhans-Cell ; diagnosis ; Humans ; Male ; Prognosis

OBJECTIVE To study the epidemiology of drug-resistant and antiseptic-resistant genes of(productive)(enzyme) in meticillin-resistant coagulase negative Staphylococcus(MRCNS).METHODS mecA Gene of the(drug-)resistant ?-lactam,aac(6′)/aph(2″) and aph(3′)-Ⅲ genes of aminoglycoside-modifying enzyme(AME) and(ermA/B/C) genes of erythromycin methyltransferase were detected by polymerase chain reaction(PCR) in 40 MRCNS strains.RESULTS From them there were 39 strains with mecA gene,32 strains with aac(6′)/aph(2″) gene,15 strains with aph(3′) -Ⅲ gene,30 strains with ermA/B/C gene,2 strains with tetM and 6 strains with qacA/B gene.There were 26 strains(65.0%) simultaneously with mecA,aac(6′)/aph(2″)and(or) aph(3′)-Ⅲ and ermA/B/C genes in MRCNS.CONCLUSIONS The drug-resistant rate is higher in MRCNS.There are more than half tested strains simultaneously with 3 to 4 drug-resistant genes.

Objective To establish a method for the determination of content in Sinomenine Hydrochloride external applied powder. Methods Diamonsil ODS C_(18) (250 mm ? 4.6 mm, 5 ?m) column was used in HPLC with mobile phase of phosphate buffer (0.01 mol/L K_2HPO_4 solution, and 1%0 triethylamine, pH was adjusted to 3.0)-MeOH (80 : 20). The flow rate was 1.0 mL/min and the detection wavelength was 265 rim. Results The linear range of Sinomenine Hydrochloride was 0.446～4.46 ?g and the regression equation was Y=9.01 ? 10~6X+102803 (r=0.9999). The average recovery was 99.2%, RSD=0.68% (n =9). Conclusion This method is rapid and accurate with good reproducibility. It can be applied to control the quality of Sinomenine Hydrochloride external applied powder.

Objective To study the chemical constituents of Zanthoxylum dimorphophyllum and identify the chemical structures.Methods The compounds were isolated by silica gel,Flash column chromatography and purified by crystallization.Their structures were elucidated by spectral methods.ResultsSeven compounds were isolated and identified.They are 6-(3′,methyl-2′,3′-dihydroxy) butyl-7-methoxyl-8-(3″-methyl-2″-butenyl)-coumarin(Ⅰ),8-(3′-methyl)-2′,3′-butenyl-2′-(1″-hydroxy-1″-methyl)-ethyl-6,7-dihydrofurancoumarin(Ⅱ),6,7,8-trimethoxycoumarin(Ⅲ),scoparone(Ⅳ),umbelliferone(Ⅴ),canthin-6-one(Ⅵ),and syringaresinol(Ⅶ).Conclusion Compound I is a novel compound and all compounds are obtained from Z.dimorphophyllum for the first time.

Objective: To compare the identification effects of different pattern recognition methods combined with chemical fingerprints on Tetrastigma hemsleyanum from different habitats, and propose a new identification method for the habitats of T. hemsleyanum. Methods: A total of 72 batches of T. hemsleyanum samples were collected from Zhejiang, Yunnan and Guizhou. HPLC fingerprints were collected, 18 common peaks were marked, and the difference of principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA) and random forest (RF) in processing complex data of samples from different habitats was compared. Results: A total of 72 batches of T. hemsleyanum samples can only be divided into two categories by PCA, the results of OPLS were better than PCA, and the RF can completely separate the samples from three habitats. The RF combined with fingerprint can effectively identify and distinguish T. hemsleyanum from different habitats. Conclusion: This study can be used as an effective method for the quality control of T. hemsleyanum from different habitats and provide an effective reference for multi-index complex fingerprint identification from different habitats.