Objective To explore the relationship among family functioning,coping style,and psychopathic personality for adult male prisoners.Methods 328 adult male prisoners were surveyed with Family APGAR Index,Simplified Coping Style Questionnaire and Psychopathic Deviate Scale.Different demographic variables were compared on male prisoners'family functioning,coping style and psychopathic personality.Coping style and psychopathic personality were compared among family functioning groups,then a relationship model was set up by structural equation modeling.Results The low age male prisoners showed significant lower growth (1.17 ±0.76),resolve (1.11 ±0.79),negative coping style(1.15 ± 0.51),the total scale of family APGAR (5.61 ± 2.73) and higher psychopathic personality (22.11 ± 4.95) than the high age male prisoners ((1.35 ± 0.72),(1.45 ± 0.68),(1.30±0.58),(6.29 ±2.63),(19.40 ±5.06),t=2.17,4.15,2.51,2.31,4.89).The rural male prisoners showed significant lower adaptation (1.04 ± 0.69) and higher positive coping style (1.95 ± 0.40) than the city male prisoners ((1.21 ± 0.74),(1.81 ± 0.43),t =2.07,3.09).The positive coping style (P =0.024)and psychopathic personality (P =0.000) of adult male prisoners among family functioning groups showed significantly difference.The strongest positive effect that adult male prisoners' positive coping style forecast family functioning was 0.275.The strongest negtive effect that male prisoners' psychopathic personality forecast family functioning was 0.257.The strongest negtive effect that male prisoners' negative coping style forecast psychopathic personality was 0.197.The strongest positive effect that male prisoners' family functioning forecast psychopathic personality was 0.266.Conclusions Positive coping style and good family functioning could effectively adjust adult male prisoners' personality.Positive coping style and perfect personality could effectively improve adult male prisoners' family functioning.Family functioning and psychopathic personality interact with each other.

Objective To explore the relationship among family functioning,positive and negative affect in male prisoners, in which the mediating effect of neuroticism and self-acceptance.Methods 315 male prisoners were surveyed with Family APGAR Index, Positive and Negative Affect Scale, Neurotism Scale,and the Self Acceptance Questionnaire.To compare positive and negative affect,neuroticism and self-acceptance of male prisoners in different family functioning groups.In addition,structural equation modeling was established among above mentioned variables.Results (1) Male prisoners in the worst family functio-ning group showed significant difference(P<0.05) in positive affect (26.31±6.55),neuroticism (14.23± 5.11) and self-acceptance (40.58±4.31) with those in the good family functioning group ((29.88±6.48), (11.49±4.89),(42.77±4.97)) and those in the worse family functioning group ((29.80±4.49),(12.91± 4.58),(42.39±5.54)).(2) Male prisoners'family functioning was significantly related to positive affect ( r=0.28) ,neuroticism ( r=-0.30) and self-acceptance ( r=0.20) (P<0.01) .Negative affect was significantly related to neuroticism ( r=-0.25) and self-acceptance ( r=0.39) (P<0.01) .( 3) Neuroticism and self-ac-ceptance played a chain mediating effect between family functioning and positive affect (P<0.01) .Neuroti-cism also played mediating effects between family functioning and negative affect as well as between family functioning and self-acceptance (P<0.01) .Conclusion Family functioning,neuroticism and self-acceptance have significant predictive power for male prisoners'positive and negative affect.To improve family functio-ning of male prisoners is conductive to personality formation and self-acceptance,furthermore,expressing pos-itive emotion experience.

Aim To investigate the role of Wnt/β-cate-nin signaling pathway on the baicalin-induced osteo-genic differentiation in rat bone marrow derived mesen-chymal stem cells ( rBMSC ) . Methods rBMSC was isolated and cultured by adherence screening method. Alkaline phosphatase ( ALP) amount, CFU-FALP and mineralized nodules were compared between each ba-icalin group and vehicle control group at different time points. Real time q-PCR was employed to evaluate the mRNA level of Wnt signaling-related marker ( Wnt10a, GSK-3β,β-catenin and LEF1) after baica-lin treatment. Protein expression of β-catenin and Runx2 was measured by Western blot. Results Ba-icalin significantly increased ALP activities from day 3 to day 7 . The formation of CFU-FALP and mineralized nodules remarkably increased after rBMSC was treated with1, 10, 50 μmol · L-1 baicalin. mRNA levels of Wnt10a, β-catenin, GSK-3β, LEF1and osteocalcin were enhanced significantly in baicalin-treated group compared to control group. Protein expression of β-catenin and Runx2 was also elevated. Conclusion Baicalin ( 0. 1 to 50 μmol · L-1 ) promotes the osteo-genic differentiation and maturation of rBMSC, in which Wnt/β-catenin signaling pathway might be in-volved.

To reveal the underlying mechanism of doxorubicin induced hepatotoxicity, an NMR-based metabolomic approach combined with multivariate statistical analysis was used to observe its metabolic alternations of rat liver. Sixteen differential metabolites between model rats and normal rats were characterized as potential pathological biomarkers related to doxorubicin induced hepatotoxicity. Six pathways, including phenylalanine, tyrosine and tryptophan biosynthesis, valine, leucine and isoleucine biosynthesis, phenylalanine metabolism, glycine, serine and threonine metabolism, alanine, aspartate and glutamate metabolism, and tyrosine metabolism were regarded as the targeted metabolic pathways according to Metabolic Pathway Analysis (MetPA). The results suggested that the metabolic perturbations in rats with doxorubicin induced hepatotoxicity were mainly involved in amino acid metabolism, lipid pathways, purine metabolism, energy metabolism, dysfunction of biotransformation and oxidative stress. The investigation revealed the effects of doxorubicin on liver in a holistic metabolic way, which laid a foundation for further studies on its toxicity mechanism.
Animals ; Biomarkers ; metabolism ; Doxorubicin ; toxicity ; Energy Metabolism ; Liver ; drug effects ; metabolism ; Magnetic Resonance Imaging ; Magnetic Resonance Spectroscopy ; Metabolic Networks and Pathways ; Metabolomics ; Multivariate Analysis ; Oxidative Stress ; Rats

Objective To explore the function of gene phoN1 in Shigella flexneri.Method Using the λ-Red recombi-nant system, phoN1was knocked out from S.flexneri 2a strain 301.Comparative proteomics was performed to analyze the differences between mutant and wild-type strains in protein expression profiles .Sereny tests and competitive infection assays were carried out to compare the virulence of mutant and wild-type strains .Results The deletion mutant of phoN1 was suc-cessfully constructed .No significant difference between the two strains was found in the comparative proteomics analyses . The function of gene phoN1 might be unrelated to the invasion ability of S.flexneri according to the results of Sereny tests and competitive infection assays .Conclusion Gene phoN1 might be of no use for the in vitro survival and host cell invasion of S.flexneri.

Medicinal values and their chemical bases of Paris (Trilliaceae) are reviewed. Paris plants include 40 species and varieties. Among them, 18 ones are medicinal plants with similarity in traditional uses. Fourteen species have been studied phytochemically, which led to isolation of 207 compounds including 121 steroidal saponins. These saponins are major active constituents from Paris plants, which can explain the traditional uses of the plants to treat cancer, malignant boil, bleeding, gastritis, and so on. The similarity in medicinal uses and chemical constituents of Paris plants implies the possibility of resource substitution among these species. It is worth to further investigate Paris plants in chemical constituents, pharmacological activity, biological property, and toxicology.
Animals ; Drug Therapy ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Humans ; Magnoliopsida ; chemistry ; Plants, Medicinal ; chemistry

To explore novel antifatigue agents targeting with AMPA receptor, 10 compounds were synthesized and their structures were confirmed by 1H NMR, ESI-MS and elemental analysis. 1-BCP was treated as the leading compound. The antifatigue activities were evaluated by weight-loaded forced swimming test, and the AMPA receptor binding affinities were tested with radioligand receptor binding assays. The results unveiled that 5b appeared to possess potent antifatigue activities and high affinity with AMPA receptor, which deserved further studies.

OBJECTIVETo study the role of Bcl-2 and Bax protein contents and their gene expression in Al-induced neurons apoptosis.

METHODSNeurons from 0 - 3 day rats were cultured and treated with different concentrations of AlCl(3 x 6) H2O. The cell apoptosis was observed by the TUNEL method and under the scan electron microscope. Bcl-2 and Bax protein contents were detected by the immunochemistry method while their gene expressions were measured by the RT-PCR method.

RESULTS(1) DNA fractions in the TUNEL method increased with the rising aluminum concentration. Blebbings and apoptosis bodies on the surface of the neurons were clearly observed under the scan electron microscope. (2) Bcl-2 protein contents and their gene expression decreased with the rising aluminum concentration (P < 0.01, r = -0.695; P < 0.05, r = -0.647), while Bax increased at the same time (P < 0.01, r = 0.676; P < 0.01, r = 0.794), the value of Bcl-2/Bax was related with the aluminum concentration (P < 0.01, r = -0.655; P < 0.01, r = -0.777).

CONCLUSIONThe aluminum may induce neurons apoptosis. Bcl-2 and Bax protein contents and their gene expression may play an important role in Al-induced apoptosis.

Aluminum ; toxicity ; Animals ; Animals, Newborn ; Apoptosis ; drug effects ; Cells, Cultured ; Dose-Response Relationship, Drug ; Gene Expression ; drug effects ; Neurons ; cytology ; metabolism ; Rats ; Rats, Sprague-Dawley ; bcl-2-Associated X Protein ; biosynthesis ; genetics

Cold Temperature ; Dinoprost ; analogs & derivatives ; urine ; Dust ; Humans ; Interleukin-6 ; urine ; Thromboxane B2 ; analogs & derivatives ; urine ; Weather

Objective To study adrenomedullin (AM) mRNA and protein expression level in myocardium of autoimmune myocarditis animal models induced by immunization of mice with lactobacillus casei cell wall element(LCWE). Methods Forty-five Balb/c male mice were randomly divided into experimental group (n = 30) and control group (n = 15), which were intraperitoneally injected with LCWE and phosphate buffered solution(PBS) at day 0,3,5 and 10,respectively. Sera and myocardium samples were gained 14,21 and 28 days after the first immunization. AM expression levels were determined by semiquantitative reverse transcriptase-polymerase chain reaction(RT- PCR) and immunchistochemistry,and mycardial histopathological lesions were observed. The anti- myosin antibodies in different stages were examined by an ELISA. Results There were myocardial necrosis or inflammatory infiltration in the experimental group, but myocardial lesions were not found in the control group. Anti - myosin antibodies were detected in sera of experimental mice,but not in control group. Immunchistochemistry findings demonstrated that AM expression level was higher in the experimental group than in the control group( P