Objective To study the effect of sub-clinical dosage of EPO on early renal lesions of diabetic rats. Methods The rats were randomly divided into normal control group (NC), diabetic group (DM), diabetic rats treated with A-dosage EPO (DMTA), and diabetic rats treated with B-dosage EPO (DMTB). Diabetic rats induced by STZ were given EPO intraperitoneally for 10 weeks; then blood glucose, blood pressure, renal function indexes, renal morphological parameter, the expression of cell apoptosis associated protein Bcl-2/Bax and VEGF were measured. Results After treatment of EPO, renal functional and morphological indexes were improved significantly and the ratio of Bcl-2/Bax and VEGF increased, both in a dose-dependent manner. Blood sugar, blood pressure and hemoglobin concentration had no significant differences. Conclusion Sub-clinical dosage of EPO could play an advantageous role in diabetic nephropathy. Inhibition of cell apoptosis by upregulation of Bcl-2/Bax expression rate may be responsible for renal protective effects despite the upregulation of VEGF.

Objective To explore the relationship between plasma homocysteine(Hcy),folic acid,VB12 level and deep venous thrombosis(DVT).Method With case-control study design,fifty DVT patients were included in this study as case group,and 50 healthy subjects selected from the same area as control group.Epidemiological survey were made,and 4ml fasting venous blood was collected for all subjects.Radio-immunoassay and fluorescence polarization immunoassay(FPIA) were used to measure blood serum folic acid,VB12 and plasma Hcy levels respectively.The correlation between Hcy level and DVT was analyzed.Results Plasma Hcy level of subjects in case group was higher than that in control group,There was significant difference of HHcy and low serum folic acid between case and control,and the OR(95%CI) was 3.74(1.21-11.59)and 0.17(0.05-0.53) respectively.There existed correlation among Hcy level,folic acid and VB12 levels.Partial correlation analysis showed the same results between Hcy level and folic acid level,VB12 level and folic acid level,while there existed negative correlation between Hcy level and VB12 level.Conclusion HHcy and low blood folic acid level increased DVT risk.Low blood levels of folic acid and VB12 increase the risk of HHcy.

Drugs, Chinese Herbal ; therapeutic use ; Humans ; Indoles ; therapeutic use ; Liver Neoplasms ; drug therapy ; Male ; Middle Aged ; Pyrroles ; therapeutic use

[Abstract ]Objective:To estimate dental maturation norms of permanent dentition in a group of Nanjing children using Nolla's tech-nique.Methods:549 cases of panoramic radiographs (279 males and 270 females)of Nanjing children at the age range of 3 to 16 years were collected for the study.All permanent mandibular teeth on the left side (except for the third molars)were scored according to Nolla's method,and data were presented in tables and used to plot dental maturation curves for the Nanjing boys and girls.Results:Females were found to be more advanced in the degrees of dental development.Dental maturation is significantly associated with chron-ological age (for males and females:r =0.959,P <0.001 and r =0.953,P <0.001,respectively).The mathematical model of them was established,and then the fitting equation was obtained.Conclusion:The dental maturation curve and chronology of permanent dentition can provide the growth norms of permanent teeth for Nanjing children and facilitate the way for dentists to assess the growing children during diagnosis and treatment planning.

Objective To analyze the present situation about early screening of developmental dysplasia of the hip( DDH) of infants in Shapingba district of Chongqing,and to explore the methods to improve the rate of diagnosis about DDH. Methods A total of 2 066 infants among 0~6 months old living in Shapingba district of Chongqing,were examined with clinical and ultrasound method on hip. Results Six infants with DDH were discovered among the 2 066 infants,which was more common in the female baby. The deficiency of hospital in which can exam hip with ultrasound method and the deficiency about DDH were the significant factors affecting the low detection rate. Conclusion Necessary measure must be taken to improve the low detection rate of DDH.

AIM: To investigate the effect of Ginsenoside Rb1(G-Rb1) on transcription of glucocorticoid receptor ? (GR?) mRNA and expression of its protein in a acute promyelocytic leukemia cell line. METHODS: Antiproliferation effects of G-Rb1 on HL60 cells were determined by MTT. The transcription of GR mRNA and expression of GR were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, respectively. RESULTS: G-Rb1 inhibited the growth of HL-60 cell line in a time-dependent and dose-dependent manner. After 36 hours treatment with 80 mg/L G-Rb1, the expression of GR? mRNA and GR protein increased. CONCLUSION: G-Rb1 exhibits the inhibitory effects on cell growth in HL60 cells, and increases the expression of GR? mRNA and protein. [

Hepatic fibrosis in rat induced by intraperitoneal injection of dimethylnitroamine (DMN) was traeted by RNA (4 mg ? kg-1) and CoQ10(2. 5 mg ? kg-1) ,seperately. Normal and pathological groups were compared. In 30, 60 and 90 days treatment, rats were killed for ul-trastructural examination and measurement of serum enzymology, serum amino acids and the quantity of collagenous fibers. The results indicated that, in RNA-treated and CoQ10 treated groups, Aspartate aminotransferase (AST) and Y-glutamyl-transpeptidase(?-GT) were normal, the ratio of albunm to globulin (A/G) had significantly difference compared with controlgroup. In RNA-treated group,L-Alanine:2-Ox-oglutarate Aminotransferase(ALT), monoamine oxidase (MAO) and N-acetyl-?-glucosaminidase (NAG) decreased evidently, serum amino acids increased slightly. Ultrastructural and microscopical examination showed the degree of hepa-tocytic necrosis degeneration and the quantity of collagenous fibers in RNA-treated group were rather mild. It appeared that there were protection effects of RNA on liver enzymology and hepaticfibrosis.

Objective To investigate the relationship between the polymorphism of the tumor necrosis factor-αgene-308 promoter and bronchial asthma (BA)in Uighur and Kazakh population in Xinjiang.Methods PCR product sequencing method was used to detect the polymorphism distribution of TNF-αgene in 60 BA patients and 60 controls among Xinjiang Uighur and Kazakh population.Then we analyzed the association between different TNF-αgenotypes and BA in Uighur and Kazakh population.Results The distribution of GG,GA and AA genotype was significantly different between the two groups.Statistical analysis showed that BA group had a significantly higher TNF-αA allele frequency than that of the control one (P <0.05).After adjustment for sex and age,we found the A allele was a risk factor for BA pathogenesis (P <0.05).Conclusion The polymorphism of TNF-α gene-308 may be associated with the susceptibility to BA in Xinjiang Uighur and Kazakh population,so TNF-α gene-308 may be considered as a genetic marker for early identification of individuals at high risk for BA,which may play an important role in preventing the development of BA in clinical practice.

Background and Objective Hyperlipidemia is one of the most potent and best substantiated risk factors for coronary heart disease (CHD). Purified Salvia miltiorrhiza extract (PSME) had been shown to have hypolipidemic effect in animal experiments. This study aimed to evaluate its iipids modulating effects in hyperlipidemic patients. Methods In this single-blind, placebo controlled study, lipid profiles of 80 hyperlipidemic patients were checked at same conditions. They were divided into two equal groups randomly (each composing of 40 patients). They were given PSME tablet (800 mg) three times per day, or placebo tablet. All patients were put on NCEP type Ⅱ diet and six weeks later, lipid profiles were checked. Results In PSME group, total cholesterol decreased by 27.32 mg/dl (12.3% reduction), LDL-cholesterol decreased by 23.13 mg/dl (16.8% reduction) and HDL-cholesterol increased by 9.06 mg/dl (11.1%), all were statistically significant. Although triglyceride dropped by 12.12 mg/dl (5.1%) but this was not significant statistically (P=-0.34). There were no significant changes oflipids levels in the placebo group. Conclusions PSME has significant favorable effect on total cholesterol, LDL-cholesterol, and HDL-cholesterol and may be a potential agent for the treatment ofatherogenic dyslipidemia.

According to the method of puncturing the twelve Jing (well) acupoints to treat hemiplegia followYinbai (SP 1), Dadun (LR 1), Shangyang (LI 1) and Guanchong (TE 1), combining with selected acupoints according to the presenting syndromes, to treat 114 cases of hemiplegia following a stroke, the total effective rate was 91.1%.