Arsenic ; blood ; urine ; Environmental Monitoring ; methods ; Humans ; Spectrometry, Fluorescence ; Spectrophotometry, Atomic

Acupuncture Therapy ; Adolescent ; Craniocerebral Trauma ; complications ; Humans ; Male ; Optic Nerve Injuries ; etiology ; physiopathology ; therapy ; Vision, Ocular

The realization of symptom measurement of the traditional Chinese medicine is a must for the development of traditional Chinese medicine, which obtained a common acceptation academically. This article believed the methods for realizing symptom measurement are the combination of both TCM two basic theories of "the wholism conception" and "treatment based on syndrome differentiation" and modern scientific measures and methods. Meanwhile, the western medicine should be simply excluded, other than simply compare a TCM syndrome to a physiological and biochemical indicator of western medicine.

Academe hash't got the same view about science attribute of traditional Chinese medicine(TCM)up to now.This article demonstrates the scientific attribute of the traditional Chinese medicine from the nature of science,the seientific attribute of medical science and the relationship between the traditional Chinese medicine and medical science.TCM needs development.In order to develop the traditional Chinese medicine quickly,on the basis of inheriting tradition,we should make the best use of modem scientific technology.Not simply copy the theories and methods of western medicine but using the scientific measurement to build up the measurement of TCM symptoms,the relationship between symptoms and the change of the body,explore the process of physiology and pathology,and combine with western medicine to develop a mature science-medical science.

Objective To examine the impact of oncology nurses' compassion satisfaction and compassion fatigue on doctor-patient relationship satisfaction.Methods Questionnaire survey was conducted by 388 oncology nurses,the questionnaires include Professional Quality of Life Scale and DoctorPatient Relationship Satisfaction Scale.Results The total scores of compassion satisfaction was 34.22±7.19,the total scores of secondary traumatic was 26.32±6.12,the total scores of job burnout was 25.13±6.08,the total scores of doctor-patient relationship satisfaction was 4.24±0.75.Pearson correlation analysis showed that doctor-patient relationship satisfaction score was positively correlated with oncology nurses' compassion satisfaction (r=0.468,P ＜ 0.05),negatioely correlated with nurses' compassion fatigue factor scores (r=-0.182,-0.531).Regression analysis showed that compassion satisfaction,secondary traumatic,job burnout and night shift frequency were influencing factors of doctor-patient relationship satisfaction,which could explain29.2％ of the variance.Conclusions Improvement of the oncology nurses' compassion satisfaction and compassion fatigue can improve the doctor-patient relationship satisfaction and the quality of nursing service,and create a harmonious medical environment.

Objective To review the physiological characters and potential function of chondroitin sulphate proteoglycan,CSPG(NG2) glial cells in the central nervous system. Methods In conclusion of the NG2 glial properties and the global, novel researches on NG2 cells. Results Cells that express the NG2 proteoglycan comprise a unique population of glial cells in the central nervous system.Study on their role in the CNS becomes more and more popular.Previous researches mainly focus on the differentiation and distinction.Now the relationship between NG2 glial cells and neurons,and the synaptic plasticity is becoming a hot topic.Conclusion This review referred the research development of NG2 glial cells,including the differentiation,migration,their interaction with neurons,and the potential role in glial cell regeneration in disease systematically.

Objective To investigate the relationship between vascular injury of diabetic rats with hind limb ischemia and endothelial nitric oxide synthase(eNOS) uncoupling.Methods 15 SD rats were randomly divided into 3 groups:control group (n =5),diabetes group (n =5) and diabetes ischemia group (n =5).Two weeks after the diabetic rat model and hind limb ischemia of diabetic rat model were set up,Enzyme linked immunosorbent assay(ELISA) was used to detect nitric oxide(NO) expression in the rat femoral artery; Dihydroethidium(DHE) fluorescent probe was used to detect superoxide(O2-) production in the frozen section of femoral artery; and Western blot was used to examine GTP cyclohydrolase 1 (GTPCH-1)expression in femoral artery.Results ①Compared with the control group,NO expression of femoral artery in diabetes group was significantly reduced,diabetes ischemia group decreased more significantly,and the difference between the 3 groups had statistical significance(P =0.000,P =0.000,P =0.001).②Frozen section showed:compared with the control group,O2-expression of diabetes group significantly increased,but not as much as diabetes hind limb ischemia group.③Western blot results showed that compared with the control group,the expression of GTPCH-1 in diabetes group reduced,diabetes hind limb ischemia group decreased more significantly,and the difference between the 3 groups had statistical significance (P =0.001,P =0.000,P =0.012).Conclusion eNOS uncoupling is present in diabetic rats with hind limb ischemia and vascular injury.

Objective To explore the ultrasonographic, clinical and histopathological characteristics features of primary uterus lymphoma. Methods The clinical, sonographic and histopathological characteristics of nine patients who were pathologically comfirmed as primary uterus lymphoma were analyzed. Results Among the nine cases of primary uterus lymphoma, three lesions were found in the uterine bodys and 6 were found in the cervixes. Two patients with uterine body lymphoma had painless pelvic masses and six patients with cervix lymphoma had contact bleeding, including irregular vaginal bleeding in four patients. In sonograms, six lesions showed remarkable hypoechogenicity with homogeneous internal echogenicity;the margins of most lesions were circumcribed;all lesions had posterior enhancement. In color Doppler lfow imaging, abundant vascularities were detected in eight lesions. In the cases of uterine body lymphoma, all of the uterine bodys showed diffuse symmetrical enlargement without disruption of the endometria, while in ifve cases of cervix lymphoma, the tumors showed the shape of lobule. The pathological examination showed that all tumors were diffuse B-cell type non-Hodgkin lymphoma. Conclusions Primary uterus lymphoma has some clinical and ultrasonic features, including contact bleeding or irrgular vaginal bleeding, diffuse symmetrical enlargement of uterine body, complete endometria, remarkable hypoechogenicity, homogeneous echogenicity and posterior enhancement. Final diagnosis depends on the histopathology.

Objective To examine the expression of ATP-binding cassette transporter A1(ABCA1)in eukaryotie cells and the effect of arsenic resistance after the transfection of eukaryotic expression vector containing ABCA1 gene.Methods HeLa cells were transfected with the recombinant plasmid by lipofectaonmine 2000 (recombinant plasmid group),empty plasmid and untransfected HeLa cell as the control group.The level of the mRNA was examined by real-time PCR,and the expression of ABCA1 protein wag examined by Western blot,the change of cell survival rate was examined by methyl thiazolyl tetrazolium(MTT)after exposure in a series of arsenic [0(contro1),4,8,16,32,64,128 μmol/L]for 48 hours.Results Expression level of ABCA1 mRNA in recombinant plasmid,empty plasmid and untransfeeted groups was(2.09±0.08)×10-4,(0.09±0.02)×10-4,(0.08±0.02)×10-4,there was a significant difference between the groups(F=1499.23,P<0.01).The level of ABCA1 mRNA in recombinant plasmid group was higher than empty plasmid and untransfected group(all P<0.01).Western blot showed that specific protein straps existed at 254×103 in all the three groups,with a similar size to the ABCA1 protein.The amount of the recombinant plasmid group was higher than the other two groups.MTT shows that arsenic concentration at 4,8,16,32,64,128 μmol/L,the survival rates of recombinant plasmid group was(94.8±0.9)%,(86.5 ± 2.6)%, (77.8 ± 2.0)%, (56.0 ± 2.0)%, (23.8 ± 1.7)%, (18.6 ± 0.6)%, higher than that of empty plasmid group[ (85.3 ± 1.1)%, (78.7 ± 0.6)%, (67.8 ± 2.4)%, (43.2 ± 1.5)%, (14.5 ± 1.3)%, (8.0 ± 0.4)%], the difference of survival rate had a statistical signifieance(t = 18.985,6.689,5.922,9.504,9.481,32.634, all P < 0.01). Conclusions ABCA1 protein is over expressed in HeLa cells after transfect ABCA1 gene. ABCA1 protein increases resistance of arsenic in HeLa cells.

Objective To evaluate the association of serum uric acid and the incidence and prognosis of kidney diseases systematically, so as to provide reference for the treatment and prevention of kidney diseases. Methods Literatures related to the associations between serum uric acid and incidence and prognosis of kidney diseases were selected from the Chinese Biomedical Literature Database ( CBM) (January 1982 to March 2010), EMBASE (January 1966 to March 2010) and Medline (January 1950 to March 2010) for cohort studies. Two researchers independently screened the studies, assessed the risk of bias of included studies using the Newcastle-Ottawa Quality Assessment Scale for Cohort Studies and extracted data. Stata 10. 0 was used to calculate the pooled relative risk. Results Twenty-one eligible cohort studies were selected, of which 11 on incidence of kidney diseases (n =276 801 ) , and 10 on the prognosis of kidney diseases ( n = 3004). Meta analysis was performed based on data influencing incidence and prognosis factors of kidney diseases except for serum uric acid. The results showed, (1) uric acid and incidence of kidney diseases: hyperuricemia could increase the risk of kidney diseases ( RR = 1. 49, 95% CI 1. 27-1. 75) ; (2) uric acid and prognosis of patients with kidney diseases:hyperuricemia could deteriorate the kidney function (RR = 1. 35, 95%CI 1. 12-1.63) and increase the risk of mortality (RR = 1.67, 95% CI 1.29-2.16). Conclusion Uric acid is an independent risk factor for incidence of kidney diseases and poor prognosis of patients with kidney diseases. Further high quality clinical trials with long-term follow up should be conducted to determine whether lowering uric acid levels would be of clinical benefit in the prevention and treatment of kidney diseases, so as to provide direct evidence for clarifying correlation between uric acid and kidney diseases and the prevention and treatment for patients with high uric acid.