Objective:To study the effect of Vitamin E(VitE)and Sodium selenite on nonalcoholic fatty liver(NAFL).Methods:24 SD rats were randomly divided into 3 groups.The model group,treatment group and contrast group were respectively fed with high fat diet,interfering diet and normal diet.All animals were sacrificed at the end of the 5th week.The liver pathology was observed under the light microscope.Superoxide dismutase(SOD),malondialdehyde(MDA)were determined by biochemistry analysis.The expressions of nuclear factor kappa B(NF-kB)and tumor necrosis factor alpha(TNF-?)proteins in hepatocytes were examined by immunohistochemistry.Result:①compared with the contrast group,serum and liver SOD levels decreased in model group,while MDA were raised.The expressions of NF-kB and TNF-a proteins in liver tissue increased significantly in model group.②compared with the model group,serum and liver SOD level increased in treatment group,while MDA was lowered.The expression of NF-kB proteins in liver tissue was reduced in treatment group,and no significant changes occured in TNF-a protein expression.Conclusions:Combination of sufficient quantum of VitE and Sodium selenite can improve the SOD activities and reduce the expression of NF-kB proteins in liver tissue,which is possibly the important mechanism for VitE and Sodium selenite to prevent NAFL.

Objective:To do research on designing the new applied multifunctional integrated tubular pipeline in femoral artery and vein which combined with blood transfusion, fluid infusion, blood sampling, administration, measurement of central venous pressure. Methods: Pipeline is consisted of three parts, which are blood transfusion part and fluid infusion part, including three way switch and three way extended tube. The first way connected with the injection is for blood sampling and doses, the second way connected with the central venous is for measurement of central venous pressure, and the third way connected with the blood transfusion set and fluid infusion set is for blood transfusion and fluid infusion. In a standard thoraco-abdominal combined injury and revivification of 31 beagle, compare the time effect. Results: In the injury and revivification test, the average time cost in 532 times nursing care operation with the multifunctional integrated tubular pipeline is 19+5.1 seconds, the average time cost in 31 times individual nursing care operation of 5 procedure is 80+12 seconds. The multifunctional integrated tubular pipeline can quickly and effectively work on blood transfusion, fluid infusion, blood sampling, administration, measurement of central venous pressure. Conclusion:The multifunctional integrated tubular pipeline in femoral artery and vein saves time and human resources and reduces the infection rate in repeated venous puncture and works quickly and conveniently on animal experiment and acute fatal injury, so it is worthy a further clinical application.

Objective To study the expression and significance of excision repair cross complementing genes 1 (ERCCI) in the tissue of nasopharyngeal carcinoma(NPC).Methods ERCC 1 proteins was assayed with immunohistochemical method in the tissue of 103 cases of NPC and 50 cases of chronic inflammation of nasopharyngeal epithelium(CINE).Results The expression rate of ERCC1 was 45.63％ in NPCs,which was significantly lower than that in CINE (70.00％) (χ2 =8.037,P ＜ 0.05).The expression rate of ERCC 1 in NPCs had relationship with T,N staging and clinical staging as well as the presence of recurrence and metastasis (χ2 =9.007,9.992,8.343,5.792,all P ＜ 0.05),while no relationship with age and gender(χ2 =0.026,0.871,all P ＞ 0.05).The expression of ERCC1 in group of recurrence and metastasis was significantly lower than those in group of no recurrence and metastasis.Conclusion The expression of ERCC1 was significantly decreased in NPCs.ERCC1 will probably become prognostic biological parameters of NPC.

Objective To explore influence factors of gerontal patients with lung squamous carcinoma after surgical therapy . Methods 210 gerontal patients with lung squamous carcinoma were accepted surgical therapy and adjuvant therapy ,and were fol-lowed up .The survival rates of 1 ,3 and 5 years after the surgery were evaluated by Kaplan-Meier ,and influence factors were ana-lyzed by Cox regression .Results The 1 ,3 and 5 years survival rates were 89 .0% ,68 .6% and 56 .2% ,respectively .The survival rates were significantly influenced by the smoking history ,the abnormal degree of mediastinal lymph node ,TNM stage ,the class number of lymph node dissection ,and the class number of mediastinal lymph node dissection(P<0 .05) .The smoking history(χ2 =16 .198 ,P=0 .000) ,the abnormal degree of mediastinal lymph node(χ2 =8 .873 ,P=0 .003) ,TNM stage(χ2 =18 .718 ,P=0 .000) , and the class number of mediastinal lymph node dissection (χ2 =4 .897 ,P=0 .027) were the influence factors of prognostic .Conclu-sion The accurate TNM staging and mediastinal lymph node with image examination could be more appropriate to operative indica-tions ,meanwhile ,if smoking was controlled ,the class number of mediastinal lymph node dissection was more .These could be bene-ficial to the survival .

Objective To investigate the serotype distribution and antimicrobial resistance of the S.pneumoniae strains isolated from children in Humen,and evaluate coverage of the serotypes by several vaccines,especially PCV7.Methods A total of 229 S.pneumoniae strains were isolated from the children treated in our hospital during January 2011 and December 2012. Capsular typing was performed by Quellung reaction.Antimicrobial susceptibility was determined by ATB STREP 5.The data were analyzed by WHONET 5.5 software according to CLSI 2010 breakpoints.Results The main serotypes identified from the 229 S.pneumoniae strains were 19F (146,63.8%),23F (49,21.4%),6B (12,5.2%)and 14 (8,3.5%).The coverage of these serotypes was 95.2% by PCV7,95.2% by PCV11,and 97.4% by PCV13.Of all the S.pneumoniae strains,penicillin susceptible S.pneumoniae (PSSP)accounted for 92.6% (212/229),penicillin intermediate S.pneumoniae (PISP)accounted for 5.7% (13/229)and penicillin resistant S.pneumoniae (PRSP)accounted for 1.7% (4/229).About 95.6%,93.0%, 88.2%,86.5%, 7.0%, 2.2% and 0.9% of the isolates were resistant to erythromycin, clindamycin, tetracycline, trimethoprim-sulfamethoxazole,chloramphenicol,cefotaxime and amoxicillin,respectively.No strain was found resistant to vancomycin or levofloxacin.Conclusions The serotypes 19F, 23F, 6B and 14 are the main prevalent serotypes of S. pneumoniae in children in Humen.PCV7 could cover 95.2% of these S.pneumoniae,indicating its appropriateness for vaccination in Humen area.These S.pneumoniae strains were highly sensitive to vancomycin,levofloxacin and penicillin,but relatively more resistant to erythromycin, clindamycin, tetracycline and trimethoprim-sulfamethoxazole.

Objective This study compared the efficacy and safety of atorvastatin and simvastatin treatment for 8 weeks in hypercholesterolemic Chinese adults.Methods This randomized,open-lable,single center study enrolled the patients with LDL-C≥4.14mmol/L(1.6 g/L) but

Objective To study the characteristics of the binding of 125 I-VIP with VIP receptor in H22 hepatocarcinoma cells in mice and in vitro. Methods VIP was radio-labelled with carrier free 125 I with the chloramine T method and purified with sephadex G-50 column chromatography. Experiments of saturation binding and competition binding were carried to study the characteristics of the binding between 125 IVIP and the VIP receptors in the H22 cells in vitro. Experiments of dynamic biodistribution and competion distribution were performed to verify the characteristics of the binding between 125 I-VIP and VIP receptors in mice with H22 hepatocarcarcinoma. Results The labelling rate of 125 I to VIP was 73.8%. The specific radioactivity of 125 I-VIP was 18.2 TBq/mmol and the radiochemical purity was 98%. The binding of 125 I-VIP with VIP receptors in H22 cells was inhibited by non-labelled VIP in a dose-dependent manner. Two classes of specific high affinity binding sites for VIP expression in H22 cells were detected with scatchard analysis. The Kd was 1.74 nmol/L and 28.63 nmol/L and Bmax was 0.27 pmol/10 6 cells and 5.57 pmol/10 6 cells for high and low affinity binding sites respectively. Radioactivity accumulation was higher in the carcinoma tissue than in the muscles of the mice bearing H22 hepatocarcinoma in each phase (P

0.6. Conclusions Ultrasonography plays an important role in the diagnosis and conservative therapy of pregnancy within a cesarean delivery scar with placenta increta.

OBJECTIVE:To observe therapeutic efficacy and safety of Leonurus artemisia injection combined with carboprost tromethamine for preventing postpartum hemorrhage (PPH) of pregnant women after cesarean section. METHODS:177 pregnant women undergoing cesarean section were randomly divided into control group(87 cases)and observation group(90 cases). Preg-nant women of both groups received cesarean section. Control group was given intramuscular injection of oxytocin 20 U in uterine muscle wall above the incision after the foetus parturition,and given intravenous dripping of oxytocin 20 U added into 0.9% sodi-um chloride 250 mL;4 h after the operation,the pregnant women received intramuscular injection of oxytocin 10 U,every 12 hours,for 3 days. Observation group was given intramuscular injection of L. artemisia injection 2 mL and Carboprost tromethamine injection 1 mL in uterine incision after the foetus parturition;4 h after the operation,the pregnant women received intramuscular in-jection of L. artemisia injection 2 mL and Carboprost tromethamine injection 1 mL,and then give L. artemisia injectien 2 mL,ev-ery 12 hours,for 3 days. Clinical efficacies of 2 groups were observed as well as blood loss during operation,2 h after operation and 24 h after operation,the amount of hemoglobin(Hb)before operation and 24 h after operation,height of uterine fundus 3,7 d after operation,the occurrence of ADR. RESULTS:The total response rate of observation group was significantly higher than that of control group;blood loss during operation,2 h after operation and 24 h after operation,height of uterine fundus 3,7 d af-ter operation were also significantly than control group,with statistical significance(P<0.05). There was no statistical significance in Hb of pregnant women between 2 groups before operation(P>0.05). 24 h after operation,Hb of pregnant women in control group was significantly lower than before operation and observation group,with statistical significance(P<0.05);there was no sta-tistical significance in Hb of observation group between before operation and 24 h after operation(P>0.05). There was no statisti-cal significance in the incidence of ADR between 2 groups(P>0.05). CONCLUSIONS:L. artemisia injection combined with car-boprost tromethamine shows significant therapeutic efficacy for PPH of pregnant women underwent cesarean section,can signifi-cantly reduce intraoperative and postoperative blood loss,accelerates the recovery of uterus without increasing the rate of ADR.

Aging or senescence is a process in which individuals undergo an exponential decline in vitality, leading to death. Recent years,much progress on the molecular mechanisms underlying senescence have been made. (1) Some senescence-related gene such as SEN6A,hic-5,din1 and MORF 4 have been clarified; (2) In 1997, through a set of experiments sponsered by scientists of Department of Biology Massachusetts Institute of Technology, it was found that the accommulation of extrachromosomal rDNA circles (ERC) in budding yeasts nucleolus is responsible for cell-senescence and the researchers propose that when enough of these circles accumulate, they clog the nucleus and prevent the cell from reading or replicating its genome, causing it to stop dividing and ultimately to die; (3) In another work finished by National Institute on Aging and the Geron biotech company of Melo, it was proved that a cells biological clock,which tells the cell how and how many times to divide, lies in its telomeres, little bits of DNA that coat the tips of the chromosome and it was clarified that a powerful enzyme,telomerase, with the potential to rejuvenate the human bodys aging tissues could effectively extend the shortened telomere . Although there is a long way to go, scientists still believe that it will be made reality in the future to greatly extend the life-span of human.