Objective:To study the effect of Vitamin E(VitE)and Sodium selenite on nonalcoholic fatty liver(NAFL).Methods:24 SD rats were randomly divided into 3 groups.The model group,treatment group and contrast group were respectively fed with high fat diet,interfering diet and normal diet.All animals were sacrificed at the end of the 5th week.The liver pathology was observed under the light microscope.Superoxide dismutase(SOD),malondialdehyde(MDA)were determined by biochemistry analysis.The expressions of nuclear factor kappa B(NF-kB)and tumor necrosis factor alpha(TNF-?)proteins in hepatocytes were examined by immunohistochemistry.Result:①compared with the contrast group,serum and liver SOD levels decreased in model group,while MDA were raised.The expressions of NF-kB and TNF-a proteins in liver tissue increased significantly in model group.②compared with the model group,serum and liver SOD level increased in treatment group,while MDA was lowered.The expression of NF-kB proteins in liver tissue was reduced in treatment group,and no significant changes occured in TNF-a protein expression.Conclusions:Combination of sufficient quantum of VitE and Sodium selenite can improve the SOD activities and reduce the expression of NF-kB proteins in liver tissue,which is possibly the important mechanism for VitE and Sodium selenite to prevent NAFL.

Objective To investigate the interaction between endometrium and embryo under the co-culture condition during the planting window stage. Methods Twenty patients who cancelled transplant for OHSS in the Reproductive Center of the second affiliated hospital of Zhengzhou university were enrolled.The discarded embryos were used to build the embryos co-culture system (Group A), with a single membrane (group B) and a single embryo(group C)as the control group. Levels of LIF and IGF expression and embryo growth were checked on 3, 4,5,6,7 day post-oocyte retrieval. Results Expressions of LIF mRNA and IGF mRNA in Group A were significantly higher than those in Group B. Embryo growth in the Group A was better than that in Group C. Conclusion Co-culture of endometrium and embryo supports the development of embryo and improves the receptivity of endometrium.

Objective To discuss the effect of the PDCA cycle in enhancing the case care ability of nurses in order for nurses to adapt to the assessment model of cases tracking.Methods The training was carried out for enhancing nurses' ability of case care according to the PDCA cycle.Results The scores of the case care,the quality of care in wards and the patient satisfaction degree increased significantly after applying the PDCA cycle compared with those before.The aims of ensuring medical safety,improving the work quality,and increasing patient satisfaction were achieved.Conclusions Training nurses' ability of case care following the PDCA cycle can effectively improve the nurses' ability of case care,make them adapt to the case tracking assessment model,which is worthy of promotion.

Mesenchymal stem cells (MSCs) play a neuroproteetive effect via a variety of mechanisms.They provide a new idea for the treatment of cerebral ischemia.However,the inadequate sources have significantly limited the possible clinical applications.Carbon nanotubes (CNTs),a new nanomaterials,can not only promote the adhesion,proliferation and differentiation of MSCs in vitro,but also as the cell carriers they can provide good microenvironmental guarantee for the survival of MSCs through the regulation of secretion of cytokines and neurotrophic factors,as well as regulation of biological characteristics of neurons,glial cells,and macrophages after their cell transplantation,provide a good microenvironment guarantee for the survival of MSCs,and promote the effect of MSCs on the therapeutic effect of cerebral ischemia.

Induced pluripotent stem cells can differentiate into a variety of cell types,which promote the development of human disease model,drug toxicity screening and sources of autologous cells.However,there have been many problems in the induced pluripotent stem cells reprogramming,such as safety and low efficiency.Small molecules are considered as a promising method to improve the reprogramming processes of induced pluripotent stem cell,and more and more small molecules have been identified to maintain stem cell self-renewal,providing a new approach to produce the desired reprogramming cells.

Objective To establish a specific,stable and reliable real-time fluorescence quantitative PCR for detecting plasma mi-croRNAs(miRNAs).Methods The plasma samples from 10 healthy individuals were collected,and miRNAs was extracted using mirVanaTM PARIS kit.Exogenous cel-miR-39 and cel-miR-238 and endogenous plasma miRNAs were reversely translated by spe-cific stem-loop primers and quantified by real time fluorescence quantitative PCR.Results cel-miR-39,cel-miR-238 and miR-342-3p were amplified and quantified specifically in RNA preparations isolated from plasma samples of healthy individuals.The amplifica-tion products of cel-miR-39,cel-miR-238 and miR-342-3p showed a single melting peak at 81.44,81.62 and 82.71 ℃,respectively, without primer dimer peak or non-specific peak in all 10 cases of healthy individual plasma samples.The standard deviation(SD)of intra-assay and extra-assay of miR-342-3p was 0.13-0.20,and the coefficient of variation(CV)was 0.42%-0.66%,which sug-gesting that this detection method has a good repeatability.The levels of miR-342-3p were detected in a same plasma sample,each experiment was repeated for 5 times,and normalized by cel-miR-39 and cel-miR-238.The SD and CV of ΔCt was 0.22,1.68%,re-spectively,which indicating that cel-miR-39 and cel-miR-238 could be taken as the stable exogenous reference for the plasma miR-NAs detection by real-time fluorescence quantitative PCR.Conclusion Real-time fluorescence quantitative PCR could serve as a good platform for plasma microRNA research.

0.6. Conclusions Ultrasonography plays an important role in the diagnosis and conservative therapy of pregnancy within a cesarean delivery scar with placenta increta.

The elements in blood or serum, including calcium, magnesium, copper, zinc, chromium, manganese, nickel, selenium, lead and cadmium were determined in the normal and patients in Nanjing by atomic absorption spectrophotometry. The method used was proved to be reliable being checked by the reference bovine serum and the data obtained in this paper was coincident with those reported by the other authors previously. The relationship between the concentrations of elements and the ages as well as the sexes in the normal was discussed briefly. The concentrations of several of the elements of the patients were significantly different from those of the normal in the same ages.

Apolipoprotein M-sphingosine-1-phosphate axis ( apoM-S1P axis ) signaling pathway consists of apolipoproteinM (apoM), sphingosine-1-phosphate (S1P) and sphingosine-1-phosphate receptor (S1PR).Plasma apoM belongs to lipocalinsuperfamily members , and is mainly associated to high density lipoprotein( HDL), whereas HDL-cholesterol correlates inversely with cardiovascular risk .The ability of apoM to bind S1P is due to a lipophilic binding pocket within the lipocalin structure of the apoM molecule . S1P, a bioactive mediator of phospholipid metabolism , predominantly abound in HDL among all lipoproteins.S1P can not only be used as intracellular second messengers , but also as intercellular signal molecules, activating of G protein-coupled receptors (S1PR) to mediate various physiological functions.It′s clear that apoM protects human beings from atherosclerosis .Furthermore, recent studies showed that S1P has a significant impact on atherosclerosis , and ApoM-S1P axis may play a important role in the pathogenesis or progression of atherosclerosis .

Aging or senescence is a process in which individuals undergo an exponential decline in vitality, leading to death. Recent years,much progress on the molecular mechanisms underlying senescence have been made. (1) Some senescence-related gene such as SEN6A,hic-5,din1 and MORF 4 have been clarified; (2) In 1997, through a set of experiments sponsered by scientists of Department of Biology Massachusetts Institute of Technology, it was found that the accommulation of extrachromosomal rDNA circles (ERC) in budding yeasts nucleolus is responsible for cell-senescence and the researchers propose that when enough of these circles accumulate, they clog the nucleus and prevent the cell from reading or replicating its genome, causing it to stop dividing and ultimately to die; (3) In another work finished by National Institute on Aging and the Geron biotech company of Melo, it was proved that a cells biological clock,which tells the cell how and how many times to divide, lies in its telomeres, little bits of DNA that coat the tips of the chromosome and it was clarified that a powerful enzyme,telomerase, with the potential to rejuvenate the human bodys aging tissues could effectively extend the shortened telomere . Although there is a long way to go, scientists still believe that it will be made reality in the future to greatly extend the life-span of human.