Objective To investigate the expression of C-C chemokine ligand 5(CCL5) in head and neck squamous cell carcinoma(HNSCC),and explore the effect of CCL5 on the biological characteristics of laryngeal carcinoma cells.Methods Gene Expression Profiling Interactive Analysis(GEPIA) database was used to investigate the expression of CCL5 in HNSCC.The laryngeal carcinoma cells TU177 were transfected with siRNA(siRNA group),and the control(NC) group was set up.The cell proliferation,migration,cycle and apoptosis of each group were detected by CCK8 assay,cell scratch test and flow cytometry respectively.RT-PCR and Western blot were used to detect the knock-down efficiency of CCL5 and the mRNA transcription and protein expression of multidrug resistance protein 2(MRP2) and bcl-2-associated x protein(Bax).Results The expression of CCL5 in HNSCC was higher than that in normal tissues(P <0.05).Compared with NC group,siRNA showed higher knock-down efficiency(t=12.898 and 22.656 respectively,each P <0.01);siRNA interference with CCL5 inhibited the proliferation and migration of laryngeal carcinoma cells,and promoted the late apoptosis of laryngeal carcinoma cells and the expression of apoptosis protein Bax(t=2.600～11.667,each P <0.05).Conclusion CCL5 was highly expressed in HNSCC,while siRNA interference with CCL5 inhibited the proliferation,migration and promoted apoptosis of laryngeal carcinoma cells TU177 by up-regulating the expression of Bax,which laid a foundation of the possibility of CCL5 as a new target for the treatment of laryngeal carcinoma.

Humans ; International Classification of Diseases ; International Cooperation ; Occupational Diseases ; classification

Objective To investigate correlation between vitamin D and glucose metabolism,lipid metabolism, insulin resistance,early secretory function of pancreatic islets in postmenopausal women with type 2 diabetes. Methods 115 postmenopausal women with type 2 diabetes were selected as observation group.115 postmenopausal women without type 2 diabetes were selected in hospital at same period as control group.All patients received detection of vitamin D,glucose metabolism,lipid metabolism,insulin resistance,early secretory function of pancreatic islets. Correlation between vitamin D and glucose metabolism,lipid metabolism,insulin resistance,early secretory function of pancreatic islets were analyzed.Results Fasting blood glucose,postprandial 2h blood glucose,glycosylated hemoglobin, body mass index,total cholesterol,triglyceride,low density lipoprotein cholesterol,insulin resistance index in the observation group were higher than the control group [(8.5 ±1.2)mmol/L vs (5.1 ±0.6)mmol/L,(13.4 ± 1.5)mmol/L vs (7.8 ±0.7)mmol/L,(8.7 ±1.1)% vs (5.6 ±0.5)%,(25.7 ±1.0)kg/m2 vs (22.3 ±0.4)kg/m2 , (5.6 ±0.7)mmol/L vs (4.5 ±0.4)mmol/L,(2.3 ±0.5)mmol/L vs (1.7 ±0.3)mmol/L,(3.5 ±0.6)mmol/L vs (2.2 ±0.4)mmol/L,(3.6 ±0.5)vs (2.5 ±0.4),t =9.667,10.179,8.535,4.524,5.443,6.529,8.909,7.406, all P <0.05].High density lipoprotein cholesterol,insulin first secretion phase peak factor,pancreatic islet βcell function index,fasting insulin,vitamin D in the observation group were lower than the control group [(1.0 ± 0.2)mmol/L vs (1.2 ±0.3)mmol/L,(5.2 ±1.3)vs (8.4 ±1.6),(135.8 ±11.7)vs (194.3 ±19.2),(4.1 ± 0.9)IU /mL vs (10.8 ±0.6)IU /mL,(10.8 ±2.1)ng/L vs (27.4 ±3.8)ng/L,t =5.087,9.153,7.370,10.414, 18.371,all P <0.05].Vitamin D was significantly negatively correlated with fasting blood glucose,postprandial 2h blood glucose,glycosylated hemoglobin,body mass index,total cholesterol,triglyceride,low density lipoprotein choles-terol,insulin resistance index(r =-0.537,-0.512,-0.469,-0.387,-0.325,-0.316,-0.372,-0.457,all P <0.05).Vitamin D were significantly positively correlated with high density lipoprotein cholesterol,insulin first secretion phase peak factor,pancreatic islet βcell function index,fasting insulin(r =0.351,0.418,0.674,0.316,all P <0.05).Conclusion Vitamin D deficiency in postmenopausal women with type 2 diabetes may be involved in glucose -lipid metabolism and insulin resistance,but also may be one of the reasons leading to decreasing of early secretory function of pancreatic βcells.

Objectlive To explore the health effects of passive smoking on children. Methods During the period of March 1,2000-Feb. 28, 2001, the frequencies of respiratory diseases (eg. cold,chathitis,pneumonia and asthma)were investigated among 1031 4-6-year old children in 4 day-care nursing schools in Lanzhou, 485 children of which were selected for the determinations of the concentration of sulphocyanate,bacteriolytic enzyme and immunoglobulin (IgG, IgA, lgM)in saliva. Results The proportion of the numbers of children suffering from respiratory diseases and cold at least 4 times per year to all of 1031 children increased with the increase of intensity of passive smoking exposure (respiratory disease: X2=13,8, P

Primary biliary cirrhosis (PBC)is a chronic disease characterized by progressive destruction of intrahepatic small bile ducts, which may progress to liver cirrhosis.Anti-mitochondrial antibodies,especially anti-M2 antibody,have a high diagnostic value for PBC, but they are unrelated to the severity and prognosis of the disease and are negative in some patients.There have been reports from around the world that anti-nuclear antibodies,especially anti-gp210 antibody,are closely associated with PBC.It showed that anti-gp210 antibody has high specificity and sensitivity for the diagnosis of PBC,especially for the patients with negative anti-M2 antibody tests;in addition,it has a high predictive value for the prognosis and development model of the disease.Anti -gp210 antibody has a high diagnostic value for PBC,with great clinical significance,so its detection holds promise for clinical application.

Objective To investigate the imaging features of solitary plasmacytoma of bone(SPB),to improve the ability of imaging diagnosis of SPB.Methods Retrospective analysis by surgery or biopsy pathology confirmed 16 cases of patients with SPB clinical and imaging data.Results All lesions were single.X-ray and CT scan showed swelling bone destruction,boundary more clearly,in all cases no periosteal reaction.Compared with the muscle signal,T1-weighted images showed equal or slightly lower signal,T2-weighted images showed high signal.All patients had no significant peritumoral edema in the bone marrow,but were seen varying degrees of soft tissue invasion.Enhanced MR scan,most cases showed significantly enhanced uniformity.Conclusions SPB presents osteolytic destruction,expansive growth and soft tissue mass without bone reaction and peritumoral edema.X-ray,CT and MR together might improve the diagnostic accuracy of SPB.

Tight junctions(TJ) are the primary junctions between intestinal epithelia.TJ serves as the maintenance of epithelial cell polarity and the rate-limiting barrier to passive movement of hydrophilic solutes across intestinal epithelia.The disrupture of TJ may cause or promote some diseases.This review is about structure,biological functions,molecular regulating mechanisms of intestinal epithelial tight junctions and some related diseases.

Objective To establish an effective method of isolating and culturing circulating fibrocytes from human peripheral blood and study the relationship between the expression specific molecule markers expression and the morphological characteristics. Methods Total peripheral blood leukocytes were isolated from human peripheral blood by being centrifuged over Ficoll-Paque and cultured in DMEM supplemented with 20% fetal calf serum.Adhered cells were detected with immunocytochemistry,FCM and electron microscopy.The collagen synthesis was studied by measurement of the hydroxyproline concentration in the medium with chemical method. Results After 9 days cultue in vitro,CD34,CD45 and collagen Ⅰ staining was positive and 83.5% of these cells secreted collagen Ⅰ detected by FCM.Electron microscopy of circulating fibrocytes showed morphological characteristics of fibroblasts.The hydroxyproline concentration in the medium was 11.17%mg/L,which was statistically and significantly different compared with 8.07mg/L in the control medium(P

Objective:To investigate the therapeutic effect of scalp multiple needle insertion by electric stimulation in the treatment of ischemic apoplexy. Methods: Scalp multiple needle insertion by electric stimulation was used to treat ischemic apoplexy and changes in rheoencephalogram, blood rheology, blood lipid and serum endothelin were observed. Results:The therapeutic effect was remarkable(P＜0.05) in the observation group and there were obvious changes in rheoencephalogram, blood rheology, blood lipids and serum endothelin (P＜0.05). Conclusion: Scalp multiple needle insertion by electric stimulation has an exact effect in the treatment of ischemic apoplexy. The therapeutic mechanism is related to improvement in blood circulation.

Objective To determine the association between serum potassium level and the severity of coronary artery atherosclerosis. Methods A total of 246 patients underwent coronary artery angiography were included into this study, and were divided into four groups according to the involved main coronary artery:control group (0 diseased vessel, n=81), one dis?eased vessel group (n=43), double diseased vessel group (n=46) and three diseased vessel group (n=76). Patients were also di?vided into low potassium group (K+<4.0 mmol/L, n=99) and high potassium group (K+≥4.0 mmol/L, n=147) according to the levels of serum potassium. The severity of coronary stenosis was quantitated by Gensini score system. The clinical data were compared between groups. The relationship between Gensini score system and clinical information was analyzed. The multi?ple regression method was used to analyse the risk factors of coronary heart disease (CHD). Results (1) There were higher percentages of elders, male, smoking, diabetes mellitus and hypertension patients in three diseased vessel group than those of control group. The serum potassium level was significantly lower in three diseased vessel group (3.97 mmol/L±0.37 mmol/L) than that of control group (4.11 mmol/L±0.33 mmol/L) and one diseased vessel group (4.13 mmol/L±0.41 mmol/L). There were higher percentages of elders, male and hypertension patients in double diseased vessel group than those of control group. The percentages of male and hypertension patients were higher in one diseased vessel group than those of control group. The Gensini scores were three diseased vessel group>double diseased vessel group>one diseased vessel group>control group (P<0.05). (2) There was a significantly higher Gensini score in low potassium group [36(8, 94)] than that of high potassium group [16(0,56)]. (3) There was significant negative correlation between serum potassium level and Gensini score (r=-0.206, P=0.001). (4) It was found that age, male, the low level of serum potassium, diabetes mellitus and hyperten?sion were independent risk factors of CHD. Conclusion The serum potassium level is negatively correlated with the severi?ty of CHD. The low level of serum potassium is an independent risk factor of CHD.