Objective To study the expression of red blood cell keratin 19(CK19) mRNA in the peripheral blood of the pa‐tients with colorectal cancer and the relationship between the CK19 mRNA and the pathological characteristics ,to investigate the relationship between CK19 and colorectal cancer .Methods Clinical data of patients with colorectal cancer received treatment at our hospital from 2012 to 2014 was retrospectively analyzed .A total of 160 patients were retrospective analyzed ,including 52 patients with colorectal cancer ,55 with colon polyps and 53 normal persons .The control group was the healthy subjects and the patients with colon polypsc who accept the physical examination at our hospital at the same period .The different expressions of CK19 mR‐NA of colorectal cancer ,colon polyps and the normal persons were compared .The relationship between the CK19 mRNA and the pathological characteristics of colorectal cancer was analyzed .The different diagnostic values of CK19 mRNA and CEA ,CA199 to colorectal cancer were compared .Results There were significant differences in the positive rate of CK19 mRNA between three groups(χ2 =54 .53 ,P<0 .01) .The positive rate of CK19 mRNA in the patients with colorectal cancer was the highest .The positive CK19 mRNA in the patients with colorectal cancer of different Dukes typing(χ2 =16 .14 ,P<0 .01) ,differentiation(χ2 =8 .155 ,P=0 .017) ,liver metastasis(χ2 =13 .68 ,P<0 .01) were different .And the differences were significant .The result of ROC area under the curve was 0 .947 which described the diagnostic value of CK19 mRNA to the patients with colorectal cancer .The sensitivity and specificity of CK19 mRNA were higher than both of CEA and CA199 .Conclusion The expression of CK19 mRNA in colorectal cancer is significantly increased .There is significant correlation between the expression level of CK19 mRNA and the staging ,grad‐ing and transferring of the tumor .The diagnostic value of CK19 mRNA to the patients with colorectal cancer is better than CEA and CA199 .

Flow-diverting stent that appears in recent years is a new method for the treatment of complex intracranial aneurysms.This article reviews the principles,composition,development,advantages and disadvantages,indications,main complications and some problems of flow-diverting stent.

[Summary] From November 2013 to March 2014, 3D laparoscopic operations were carried out in 14 cases in the department of urology of our hospital .The operation went smoothly , without conversions to laparotomy .The operation time was 54-466 min, with an average of 180 min.The intraoperative blood loss was 5 -400 ml, with an average of 70 ml.No pressure sores or operation complications occurred.The medical staff participating in the operation generally felt a good 3D vision, without sense of vertigo.We deem that during urological operations which have different operation postures , it is very important to protect both laparoscopic system and operation area from pollution .Timely and properly wearing 3D glasses, application of one-eye-detection method for lens sharpness , and the switching between 2D and 3D modes can reduce and relieve visual fatigue .

Objective To investigate the relationship between helicobacter pylori(HP)eradication and curative effect of gastroe-sophageal reflux disease(GERD) .Methods Two hundred and fifty four patients with GERD were prospectively recruited for endo-scope and acid reflux evaluation .According to the results of gastroscope ,the eligible patients were divided into non-erosive reflux disease (NERD) and erosive esophagitis (EE) group ,and each divided into HP positive and HP negative group ,the HP positive group were randomly divided into the anti HP treatment group and not .These patients were assigned to rabeprazole triple therapy (the anti HP treatment group) or rabeprazole(20 mg ,twice per day) single(other groups) for 10 d ,then rabeprazole (20mg ,twice per day) was given to all patients until two months .Then all patients underwent acid reflux evaluation ,EE group underwent endos-copy ,and all patients underwent a 13 C urea breath test 4 weeks after cessation of rabeprazole to determine HP status .Results One hundred and seventy nine GERD patients were included in the study(NERD group 119 cases ,including anti HP treatment group 47 cases ,not anti HP treatment group 32 cases ,HP negative group 40 cases ;EE group 60 cases ,including anti HP treatment group 24 cases ,not anti HP treatment group 15 cases ,HP negative group 21 cases) .HP was eradicated in 76 .6% of the NERD anti HP treatment group and in 75 .0% of the EE anti HP treatment group .Overall ,there is no difference between the anti HP treatment group and other groups on these aspects ,including symptom、esophagitis and acid reflux improvement .Conclusion There are no significant correlation between HP eradication and GERD .

The childhood asthma is characterized as high morbidity,recurrent attack,chronic persistence and refractoriness. So asthma with these features mentioned above can have important effect on children′s emo-tional and physical development as well as their families. This review sketches the studies of children asthma family management present situation and its influencing factors,and stresses the necessity of asthma family man-agement. Methods of psychotherapy used in our country and abroad and its implementation effect are reviewed, including behavior therapy,cognitive therapy,cognitive behavioral therapy,relaxation therapy,family therapy and other existing psychological treatment,in order that these measures can be conductive to the development of pre-vention methods of pediatric asthma and further researches.

The pro-inflammation cytokine is secreted by cells,and the function is to promote and regulate inflammatory reactions.They include IL-1,IL-6,TNF-? and IFN-?.Researchs have revealed that the levels of serum pro-inflammation cytokinemight increase notably in inflammatory bowel disease patients and they could cause the change of intestinal epithelium tight junctions.

Objective The present study was to analyze the role of ACh-lateral septum(SL)pressor system in the Central amygdaloid nucleus(AC)-emotional pressor circuit.Method Interurban microinjection of different drugs,then blood pressure and heart vate were vecorded.Results(1)CRF(corticotropin releasing factor)or SP(substance P)microinjection into the SL can induced pressor responses.The AC pressor responses to glutamate(Glu)could be attenuated by preinjection of CRF antagonist or SP antagonist into bilateral SL.(2)the AC pressor responses to Glu were also reduced by preinjection of atropine into either bilateral HBL,LC or RVL respectively,(3)Since lateral HB(HBL)projects to the posterior hypothalamus(HP)containing ACh-ergic neurons,and excitation of the latter produces pressor response via the RVL and LC-RVL;atropine preinjection into the HP could also decrease the HBL-and AC-pressor response.Conclusion The present results indicate that the AC produce pressor responses involved with the SL via their CRF and SP,mechanism underlying pressor response of SL is involved in pressor response of AC.

Objective To study the effect of Iguratimod on the collagen secretion of 3T6 cells. Method The median toxic concen- tration of Iguratimod was determination by MTT Assay. Iguratimod' s median toxic concentration to 3T6 cell was about 1142.6mg/L. Taking into the consideration of the cells performance after administration under spectacles, we chosen the administration concentration as 334.8 mg/L(DMS0:1?l/256?l) ,167.4mg/L,83.7mg/L and 41.85 mg/L. After administration of 12 hours, 24 hours, 36 hours and 48 hours, the supernatant was collected for assayed respectively. The collagens content was detected by the method of chloramine T. Results The collagen concentration of the drug concentration 334.375mg/L group increased (P

Iliac vein compression syndrome is one of the most common lower extremity venous backflow obstruction disorders,which often causes lower limb swelling,venous stasis dermatitis,lower extremity deep venous thrombosis,lower extremity venous valvular insufficiency,etc.At present the commonly used imaging diagnostic means include ultrasonography,multi-slice computed tomography,magnetic resonance and digital subtraction angiography.This paper aims to review the recent progresses in the imaging diagnosis of iliac vein compression syndrome.

Objective To observe and study the distribution and histological feature of the fascicular groups to deltoid muscle in axillary nerve at the level of quadrilateral zone. Methods 12 adult human cadavers (24 upper limbs)embalmed by formalin were used,, and microsurgical longitudinal contradissection of the axillary nerves by tracing their terminal branches were processed. At the level of quadrilateral zone,distrabution of the fscicular groups to deltoid muscle in the never trunk were recorded and their diameters were measured;Another 6 upper extremities (3 right and 3 left) of fresh-frozen human cadavers were obtained.Axillary nerves and their fascicular groups were exposured, and the segments at the level of qusdrilateral zone gotten, then histological cross section was done, acetycholinesterase (ACHE) stained with the method of Karnovsky Roots and myelin stained with the method of Loyezs. Defferent never fibres were distinguished under microscope and the fiber number was counted with IAS . Results At the level of quadrilateral zone,fasciculars were divided into two groups.The fascicular group of anterior branch to deltoid muscle was in the lateral of the nerve trunk . Its cross sectional area was (2.449?1.327)mm2 ,occupied 55.4%?9.3%of the axillary nerve trunk ;Its most fibers were motor fibers;The number of fibers is (2112?631), occupied 45.6 %?1.1%of all fibers of axillary nerve . Conclusions In the treatment of root avulsion of brachial plexus injury, selective suture donor nerve with the lateral fascicular group should be processed to repair the function of shoulder abduction,fibers loss would be reduced and rate of functional restoration improved .