1.Senescence Accelerated Mouse Prone 8 for Study of Alzheimer's Disease (review)
Yan-zheng LI ; Lin LI ; Lan ZHANG
Chinese Journal of Rehabilitation Theory and Practice 2012;18(12):1119-1122
Alzheimer's disease (AD) is a neurodegenerative disorder characterized by memory loss and cognitive decline. The pathological eatures of Alzheimer's disease are abnormal deposition of amyloid beta-peptides (Aβ), neurofibrillary tangles, cholinergic deficits, and loss of synaptic processes and dendritic spines. Senescence accelerated mouse prone 8 exhibits age-related deficits of learning and memory from an early age, tau protein phosphorylation, neurotransmitter changes, synaptic structure and function disorders, circadian rhythm disorders,as well as gene expression and many other characteristic changes, which are consistent with Alzheimer's disease pathological changes,and can be used as an ideal animal model for Alzheimer's disease prevention drugs development.
2.Study on indicators and evaluation of nurses' performance appraisal
Fenshan ZHENG ; Lan CUI ; Chunhua LI
Chinese Journal of Practical Nursing 2015;31(34):2647-2652
As the important content of excellent quality nursing service, the performance appraisal of nursing staff was closely interrelated to their income distribution, professional title promotion and excellence choosing. Performance appraisal was the requisite part of nursing resource management. This study gave an overview about conception and development of nursing staff′s performance appraisal and summarize key performance indicator,balanced score card,SMART principle and 360 degrees performance appraisal, which was mainly used in domestic hospital. It suggested nursing manager should have complementary advantages of them and utilize foreign mature methods to explore and improve our countries performance appraisal of nursing staff and formulate the system which was in keeping with China′s national conditions.
3.Effect of Guishen Pain-Killer on easing pain in micro-wave heating among tissue to treat hemorrhoids
Jihua LI ; Yanmei GUO ; Lan ZHENG
Chinese Journal of Rehabilitation Theory and Practice 2003;9(11):692-693
ObjectiveTo observe the easing pain effect of being used Guishen Pain-Killer, a Chinese medicine, for improving the anaesthesia project which was used in using micro-wave heating among tissue to treat hemorrhoid.MethodsOn the basic of the original anaesthesia project, patients drunk the Chinese medicine Guishen Pain-Killer 50ml which was heated to 40℃ by microwave stove for pre-anaesthesia drug 10 minutes before the operation. The effect of adding drinking Guishen Pain-Killer and of merely using the original anaesthesia project were estimated with the McGill Questionnaire and stated the data with u test.ResultsThe pain estimation of those patients who drunk Guishen Pain-Killer before operation was (1.71±1.05) and (1.32±0.83), in operation and just after operation,while that of patients who didn't drink Guishen Pain-Killer before operation was (2.58±1.03) and (1.93±1.11),P<0.01.ConclusionGuishen Pain-Killer can be used as the pre-anaesthesia drug in micro-wave heating among tissue to treat hemorrhoids.
4.Effects of apM1-contained recombinant adenovirus on malonaldehyde and superoxide dismutase levels in HUVEC cells
Bingrong LI ; Dan ZHENG ; Huacong DENG ; Lizhen LAN ; Hongting ZHENG
Chinese Journal of Endocrinology and Metabolism 2010;26(9):788-791
Objective To evaluate the effects of exogenous adenovirus-mediated gene transfer of human apM1 gene (Ad-apM1) on malonaldehyde (MAD) and superoxide dismutase (SOD) levels in human umbilical vein endothelial cells (HUVEC). Methods HUVEC proliferation was measured by MTT after infection by AdapM1. Adiponetin protein level in cell culture medium of HUVEC cells infected with Ad-apM1 was detected by double antibody sandwich ELISA. The levels of MAD and SOD were measured by chromatometry. Results MTT assay showed that Ad-apMl had no effect on HUVEC proliferation. Human adiponectin protein levels in cell culture medium significantly increased after HUVEC was infected with Ad-apM1 for 24, 48, and 72 h, along with decreased MAD and increased SOD ( all P<0.05 ). MAD levels markedly increased and SOD levels decreased after HUVEC were incubated with 100 μmol/L H2O2 for 6, 12, and 24 h, and these reactions were reversed by AdapM1 transfection (all P<0.05 ). Conclusions HUVEC infected with Ad-apM1 effectively secrete human adiponectin. Ad-apM1 exerts antioxidation effect and antagonizes H2O2 -induced endothelial injury.
5.Comparative study of different preservatives for preservation of isolated nurine heart under low temperature
Yan ZHENG ; Bojun LI ; Libing LI ; Lan MA
Medical Journal of Chinese People's Liberation Army 1982;0(01):-
Objective To compare the results of three kinds of heart preservative fluids,namely histidine-tryptophan-ketoglutarate(HTK)solution,Fuwai modified solution(FWM)and St.Thomas' Hospital cardioplegic solution number 2(St.Thomas-Ⅱ)for the preservation of isolated rat hearts under low temperature.Methods Fifty-four male SD rats were randomly divided into HTK,FWM and ST-Ⅱ groups(18 each),and each group was divided into three subgroups(6 each)according to different duration of preservation under low temperature(4,6 and 8 hours).The hearts were harvested and then mounted on the Langendorff apparatus and perfused with K-H buffer solution for determination of the baseline hemodynamic values(HR,LVSP,?dp/dtmax and CF).The isolated hearts were then arrested and stored in different preservative solution as mentioned at 4℃ for 4,6 or 8 hours.The hemodynamic values were measured before cardiac arrest and after cardiac reperfusion in Langendoff model,and the recovery rates of the values were calculated.The hearts were finally sectioned after reperfusion for the observation of morphological changes with light microscope.Results With the same hypothermic preservation time,the recovery rates of hemodynamic values were significantly higher,and the changes in myocardial structures were obviously less in HTK and FWM groups than in ST-Ⅱ group(P0.05).Conclusions HTK and FWM solutions are more effective than St.Thomas-Ⅱsolution in preservation of isolated heart under low temperature with the same storage duration.The effect of HTK solution for preservation of isolated heart descended with the prolongation of preservation time,especially so after 8 hours.
6.Expression status of HER2 in mammary and extramammary Paget's disease.
Hui MENG ; Xiang-Yu ZHENG ; Lan ZHANG ; Wen-Cai LI
Chinese Journal of Pathology 2011;40(4):255-256
Adult
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Aged
;
Breast Neoplasms
;
genetics
;
metabolism
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pathology
;
surgery
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Female
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Gene Amplification
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Genital Neoplasms, Male
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genetics
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metabolism
;
pathology
;
surgery
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Humans
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Male
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Middle Aged
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Paget Disease, Extramammary
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genetics
;
metabolism
;
pathology
;
surgery
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Paget's Disease, Mammary
;
genetics
;
metabolism
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pathology
;
surgery
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Penile Neoplasms
;
genetics
;
metabolism
;
pathology
;
surgery
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Receptor, ErbB-2
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genetics
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metabolism
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Scrotum
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Vulvar Neoplasms
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genetics
;
metabolism
;
pathology
;
surgery
7.Clinical analysis of infectious mononucleosis misdiagnosed as purulent tonsillitis.
Zhi-xiong XIAN ; Lan LI ; Yue-jie ZHENG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2007;42(6):466-467
Child
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Child, Preschool
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Diagnostic Errors
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Female
;
Humans
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Infant
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Infectious Mononucleosis
;
diagnosis
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Male
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Suppuration
;
diagnosis
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Tonsillitis
;
diagnosis
8.Prenatal Sonographic Characteristics of Clubfoot and Relationship with Chromosomal Abnormalities
Meifang LIN ; Hongning XIE ; Ju ZHENG ; Ting LEI ; Lan LI
Journal of Sun Yat-sen University(Medical Sciences) 2017;38(2):291-295
[Objective]To evaluate the incidence of chromosomal abnormalities and associated abnormalities in prenatally diag?nosed clubfoot,and to determine the prognostic factors as well.[Methods]A total of 89 fetuses with clubfoot diagnosed during Janu?ary 2010 to October 2015 in prenatal ultrasound scan and confirmed postnatally or by autopsy,were selected,within which 16 (18.0%)cases were without other abnormalities and 73(82.0%)cases were with other abnormalities. The associated abnormalities were identified ,the correlation with chromosomal abnormalities were analyzed with Fisher analysis and the factors affecting the outcomes were determined with Logistic regression analysis.[Results]Among associated abnormalities ,the skeletal abnormalities besides the clubfoot were the most frequently associated anomalies (35 ,47.9%),and the central nervous abnormalities followed secondly(30,41.1%). A chromosomal abnormality,with trisomy 18 being the most frequently detected,was identified in 34%(17/49)of the clubfoot fetuses with other anomalies ,whereas none of chromosomal abnormality was identified in 11 fetuses without other anomalies,a significant different rate of aberrant chromosome noted(P < 0.001). The survival rate of clubfoot fetuses without other anomalies was higher than that of clubfoot fetuses with other anomalies(50.0%vs 1.3%,P=0.03). The conditions of with or without associated anomalies were the independent prognostic factors (P = 0.01),the clubfoot fetuses associated with other anomalies had poor outcomes[OR=11.9(95%CI:1.8,80.1)].[Conclusion]Skeletal abnormalities besides the clubfoot were the most frequently associated anomalies. The condition of with or without associated anomalies is the independent prognostic index for fetuses with clubfoot. Aneuploidy were more commonly in clubfoot fetuses with associated abnormalities than in those without other abnormalities. No indication for karyotyping suggests for the clubfoot fetuses without other abnormalities due to the low incidence of associate chromo?somal anomalies.
9.Clinical Study on Acupuncture and Ischemic Stroke
Lan LI ; Ping ZHENG ; Xin CHEN ; Yang BAI ; Min MAO
Journal of Acupuncture and Tuina Science 2009;7(3):137-139
Objective:To study the clinical efficacy of acupuncture for ischemic stroke and its effect on ET-1 levels in IS cases.Method:The 63 cases were randomized into a treatment group (31 cases),receiving acupuncture and routine method,and control group (32 cases),receiving routine method alone.Seven days constitute a course of treatment,a 2-day interval between two courses.The clinical efficacy and ET-1 level in two groups were compared after four weeks.Result:The total effective rate in the treatment group and control group were 96.8% and 75% respectively,with a statistical significance (P<0.05);the ET-1 level in blood plasma also with a statistical significance after the treatment (P<0.05);the pre-treatment and post-treatment ET-1 level in the treatment group showed a statistical significance (P<0.01),whereas the control group didn't.Conclusion:Acupuncture is effective for ischemic stroke and can lower the ET-1 level in ischemic stroke cases.
10.The ECG tube current modulation technique of dual source CT in clinical application of coronary angiography
Guangfang TUO ; Yongshu LAN ; Guangcai TANG ; Zhenlin LI ; Lu ZHENG
Journal of Practical Radiology 2016;32(8):1289-1292
Objective To explore the application value of ECG tube current modulation technology of dual source CT in coronary angiography,compared with retrospectively ECG-gated technique.Methods 200 patients were randomly divided into ECG tube current modulation group (Group A)and retrospectively ECG-gated group(Group B).In Group A,the main parameters were the exposure time windows of full dose,which were set according to different heart rates.χ2 test was adopted in the subjective score,and t test was used in the objective score and radiation dose.Results There was no statistical significance in image quality between the two groups(χ2 =2.125,tnoise =-0.557,P >0.05 );The effective dose (ED)of Group A and Group B were (3.30±0.40)mSv and(6.90±1.76)mSv,respectively.The difference was statistically significant (t ED =-1 9.954,P <0.05).The radiation dosage of Group A was 52% lower than that of Group B.Conclu-sion Compared with retrospectively ECG-gated technique,the ECG tube current modulation technique ensures the image quality, and at the same time it can significantly reduce the radiation dose,therefore,it can be used as a routine examination technique of cor-onary angiography.