Objective:To observe the effect of ischemic preconditioning(IP)on hepatocellular apoptosis caused by ischemia-reperfus ion(IR)injury and expression of apoptosis regulatory genes(Survivin,HIF-1?)protein,to explore the protective mechanisms of IP on IR injury in rat liver.Methods:Ninty SD rats were randomly divided into sham operation(SO)group,ischemia-reperfusion(IR)group,ischemic preconditioning(IP)group.In SO group,the hepatoduodenal ligament of rat was only pulled and separated,and then closed the abdomen;IR group subjected to 30 minutes of ischemia;IP group was achieved by ischemia for 5 minutes,followed by reperfusion for 5 minutes before continuous to ischemia for 30 minutes.Ten rats of each group were killed after reperfusion for 2,6,12,24 hours respectively,then liver tissues were taken out.The rats in SO group were killed after operation for 2 hours.To detect the Apotosis index(A)Iand Survivin,HIF-1? protein levels.Results:AI of hepotocytes significantly increased in IR group and IP group than that in SO group(P

With the development of surgical techniques in dental implantation, traditional teaching methods can not keep pace with the developing teaching situations.To resolve this problem,the live television technique has been applied in the teaching of dental implant surgery.The practice of live television technique not only stimulates students' learning interests,but also turns the passive education model into an interactive model with better teaching results.

Wnt/β-catenin signaling pathway plays an important role in the initiation and progression of colorectal cancer. The key to its carcinogenicity is related to the inhibition of β-catenin degradation, and the subsequent cytoplasmic accumulation and nuclear translocation of β-catenin, which activates the transcription of downstream target genes such as coding region instability determinant-binding protein (CRD-BP). In cancercells ,the expression of CRD-BP mediates the stabilization of β-Trcp, which in normal cells regulates the degradation of β-catenin. The activation of Wnt/β-catenin signaling pathway, overexpression of β-catenin and CRDBP, and the upregulation of β-Trcp by CRD-BP play important roles in the initiation, progression, and metastasis of colorectal cancer.

ObjectiveTo observe the chronological and spatial expressions of transforming growth factor-β receptor typeⅡ(TGF-βRⅡ)and fibronectin (Fn )in developing mouse kidney and investigate the relationship between the expressions and kidney development. Methods Immunohistochemistry and Western blotting were used to observe and measure the expression of TGF-βRⅡ and Fn in embryos and postnatal mouse kidneys. Results The expression of TGF-βRⅡ was noticed in all stages of glomeruli, renal tubes and collecting ducts, and increased gradually with ages; The expression of Fn was noticed too in all stages of glomeruli, renal tubes and collecting ducts, except for the comma-shaped bodies, and also increased gradually with ages. Conclusion TGF-β and Fn might play an important role in the development and maturation of mouse kidney.

BACKGROUND: The meniscal allograft transplantation has been proved good outcome in clinical, however, the source and matching restrict its application. By means of a patent technique of separating antigen, a new biological meniscus can be obtained.OBJECTIVE: To observe the histological changes of transplanted biological meniscus in goat's knee joint, in addition, to investigate the feasibility of reconstituting meniscus by using biological meniscus. DESIGN, TIME AND SETTING: The animal experiment of goats was performed at the Central Laboratory in the Third Affiliated Hospital of Sun Yat-sen University between June 2005 and June 2006.MATEAIALS: A total of 15 healthy goats were used in the experiment. Fresh porcine meniscus was harvested for the preparing of biological meniscus.METHODS: The medial meniscus of goat was excised, and then the absent part was repaired using biological meniscus. MAIN OUTCOME MEASURES: The animals were sacrificed at months 1, 3, 6 and 12 after operation. And the samples were detected by histology, scanning electron microscopy (SEM) and Indian ink perfusion. RESULTS: The incision of all animals healed well, without infection or death. The transplanted meniscus healed well with the surrounding tissue, and the connection was tight. With time prolonged, the transplanted meniscus slowly soaked, and live cells existed inside the meniscus, but there were no live cells in about 1/4 district at 1 year after the surgery. Indian ink perfusion showed that the surrounding tissue grew inside and set up microcirculation, but only limited to 10% periphery of the meniscus (normal goat approximately 15%). The transplanted meniscus began the necrosis at 6 months after surgery. Under SEM, the surface of the meniscus became crude and cracked in some places, but still maintains the gross shape at 1 year after the surgery.CONCLUSION: There is no obvious rejection after the biological meniscus was transplanted into knee joint of goats. During the process of absorption, autologous tissue of goats can grow into and set up microcirculation in the transplanted meniscus. The transplanted meniscus protects the'knee cartilage notably, only slightly damage in cartilage can be observed.

Objective To study the association between platelet distribution width (PDW) and other indexes in coronary heart disease (CHD) patients with heart failure (HF).Methods Five hundred and ninety-one CHD patients with HF were divided into control group with their NT-proBNP＜300 ng/L (n=228),mild HF group with their NT-proBNP=300-3000 ng/L (n=191),and severe HF group with their NT-proBNP＞3000 ng/L(n=172).Their blood and echocardiographic parameters were recorded.Results The PDW was significantly shorter in 3 groups after treatment than before treatment (0.129±0.031 vs 0.133±0.027,0.133±0.030 vs 0.141± 0.029,0.139±0.028 vs 0.148±0.029,P＜0.05,P＜0.01),and was significantly longer in mild HF group and severe HF group than in control group and in severe HF group than in mild HF group after tgreatment (P＜0.05,P＜0.01).Multivariate stepwise regression analysis showed that PDW was positively associated with NT-proBNP and LAD (P=0.001,P=0.000) and negatively associated with LVEF (P=0.003).Conclusion PDW increases with the severity of HF in CHD patients and can thus play an auxiliary role in assessing the severity of their HF.

Rofecoxib, a COX-2 specific inhibitor, is approved for treatment of osteoarthritis, acute pain and primary dysmenorrhea. On September 30, 2004, Merck announced the voluntary worldwide withdrawal of rofecoxib (Vioxx). This paper reviewed the pharmacological actions of the COX-2 specific inhibitors and the characterization, premarket study, clinical application and side-effects of rofecoxib and analyzed the withdrawal of rofecoxib.

AIM:To establish the method for determining oridonin and rosemarinic acid in Donglingcao Tablets(Rabdosia rubescens(Hemsl.) Hara). METHODS:Oridonin and rosemarinic acid were determined by HPLC simultaneously with changing ultraviolet-visible wavelength.Chromatographic condition was composed of ODS-C_(18) column,methanol-03.% phosphoric acid (40∶60),UV detection wavelength of oridonin at 238 nm and of rosemarinic acid at 329 nm. RESULTS:The average recoveries were 97.8% and 96.7% and related standard deviations(RSD) were 1.9% and 2.3%. CONCLUSION:This method can be used to determine simultaneousely oridonin and rosemarinic acid in Donglingcao Tablets.

It is rare that large mixed tumors of salivary gland located in the nose and uvula. The patient who had mixed tumors of salivary gland at nose found exophytic growth tumor for half a year. The other patient who had mixed tumors of salivary gland at uvula felt swallowing foreign body sensation for one year, and dysphagia for one month. After surgical resection, the symptoms disappear.
Adenoma, Pleomorphic ; complications ; pathology ; Deglutition ; Deglutition Disorders ; etiology ; Humans ; Nose Neoplasms ; pathology ; Palatal Neoplasms ; pathology ; Salivary Gland Neoplasms ; complications ; pathology ; Uvula ; pathology

Objective To investigate the clinicopathological features,immunophenotype,diagnosis and differential diagnosis of oncocytic mucoepidermoid carcinoma(OMEC).Methods A case of OMEC was studied for clinicopathological and immunohistochemical features,the available literature on this subject was reviewed as well.Results Magnetic resonance imaging revealed a well-defined mass,measured 1 cm×1 cm,within the right submandibular gland.The patient was underwent the total mass and the right submandibular gland resections.On macroscopic examination,the well-defined tumor was gray-yellowish on cut surface with hard consistence and was sized 1 cm×1 cm×0.8 cm.The uncapsulated tumor was mainly solid with focal microcysts on cut surface.Microscopically,the tumor consisted of nests,sheets,and occasional microcysts of oncocytic cells.Focally,intermediate cells,mucilage cell and epidermoid cell were also present.Immunohistochemically,the oncocytic cells were diffusely and intensely positive for CK(AE1/AE3),EMA and P63.By histochemical staining,the oncocytic cells were positive for PTAH staining,and mucilage cell were positive for PAS staining.Conclusion The OMEC is an exceedingly rare variant of mucoepidermoid carcinoma with a good prognosis and should be differentiated from oncocytoma,oncocytic carcinoma,Warthin's tumor with mucinous metaplasia,pleomorphic adenoma,oncocytic myoepithelioma and myoepithelial carcinoma,et al.