OBJECTIVE To study the expression of c-Met,TIMP-2 in laryngeal squamous cell carcinoma and to explore the function and relationship of c-Met,TIMP-2 in the invasion and metastasis of laryngeal squamous cell carcinoma. METHODS Immunohistochemical method(SP) was used to detect the expression of c-Met,TIMP-2 in 41 laryngeal squamous cell carcinoma specimens and 15 specimens adjacent to the tumors.RESULTS The positive rate of c-Met and TIMP-2 in laryngeal squamous cell carcinoma specimens was 56.1% (23/41)and 63.4%(26/41)respectively.The positive rate of c-Met in tissues adjacent to tumors was 13.3%(2/15).No positive expression of TIMP-2 was found in tissues adjacent to tumors.CONCLUSION The expression of c-Met showed a positive correlation with histological differentiation and cervical lymph node metastasis and TIMP-2 showed a positive correlation with cervical lymph node metastasis in laryngeal squamous cell carcinoma.There was no significant relation between the expression of c-Met and TIMP-2 in laryngeal squamous cell carcinoma.

OBJECTIVE:To evaluate the economical efficiency of 3 therapeutic plans for acute upper respiratory tract infec-tion caused by group A β-hemolytic streptococcal (GAS). METHODS:In retrospective study,71 GAS positive patients with acute upper respiratory tract infection in our hospital during Mar. 2014-Mar. 2016 were selected and divided into group A(16 cases),B(30 cases),C(25 cases)according to medication method. Group A,B,C were given intramuscular injection of ben-zathine benzylpenicillin(1200000 U),oral administration of penicillin V potassium(0.5 g,q8 h,10 d)or oral administration of amoxicillin sulbactam(0.5 g,bid,10 d),respectively. Clinical efficacy and bacterial clearance were observed,and econom-ics of 3 therapeutic plans were evaluated. RESULTS:The cure rates of 3 groups were 87.50%,90.00%,and 92.00%;bacterial clearance rates were 88.24%,87.87%,and 92.59%,respectively;there was no statistical significance(P>0.05). Cost-minimi-zation analysis was adopted for pharmacoeconomic evaluation. The costs of 3 groups were 237.79,279.49,400.40 yuan,respec-tively. The cure rate and bacterial clearance rate of therapeutic plan C were the highest,but its cost also was the highest. The re-sults of sensitivity analysis were consistent with the results of cost-minimization analysis. CONCLUSIONS:The intramuscular in-jection of benzathine benzylpenicillin can be served as first choice therapeutic plan for recurrent upper respiratory tract infection caused by GAS and patients with rhenmatic fever risk. The patients who are reluctant to be treated with penicillin but guarantee medication time can take penicillin V potassium orally. The patients with poor efficacy of common penicillin can take amoxicillin sulbactam pivoxil.

In order to determine whether the variations in the calpain-10 gene constitutes risk of type 2 diabetes (T2DM) in Chinese, the frequency of UCSNP-43, 44 in 268 adults newly diagnosed with T2DM (according to the 1999 ADA criteria) and 153 non-diabetic control subjects was investigated. For all subjects, the height, weight, waist-to-hip ratio (W/H) and blood pressure, as well as following parameters were measured: (1) 75-g oral glucose tolerance test with insulin, C-peptide, HbA1c and blood lipid profiles; (2) Genomic DNA extracted from peripheral blood lymphocytes was genotyped for UCSNP-43 (calpain-10-g. 4852 G/A) and UCSNP-44 (calpain-10-g. 4841 T/C) by sequencing a polymerase chain reaction (PCR)-amplified fragment. PCR product was selected by single strand conformation polymorphism (SSCP) and then sequenced. The results showed that there was significant difference between T2DM group and normal control group in allele frequencies, haplotype frequencies, or haplotype combinations of UCSNP-43 and -44 either. But in newly diagnosed T2DM group, it was found that the individuals with the genotype UCSNP-44 T/C + C/C had significantly increased fasting and post-challenge insulin levels (Fins and P2hIns), consistent with reduced insulin sensitivity. In the BMI> 25 subgroup, the differences were even more significant. It was demonstrated that the Calpain-10 gene polymorphism UCSNP-44 was associated with insulin sensitivity and Fins and P2hIns in newly diagnosed T2DM, although Calpain-10 doesn't appear as a major diabetes susceptible gene in this population.
Asian Continental Ancestry Group ; Calpain/*genetics ; Diabetes Mellitus, Type 2/*genetics ; Gene Frequency ; Genetic Predisposition to Disease/genetics ; Insulin Resistance/*genetics ; Phenotype ; Point Mutation ; Polymorphism, Genetic/*genetics

Background The pathogenesis of allergic conjunctivitis has not been clearly established.Current researchers indicate that interleukin-4 (IL-4), IL-5 and IL-13 may play an important role in allergic conjunctivitis.But whether the roles of these inflammatory factors are same in different types of allergic conjunctivitis remains unclear.Objective This study was to investigate the expressions of IL-4, IL-5 and IL-13 in ocular surface with different types of allergic conjunctivitis.Methods A prospective cohort study was designed.Eighty individuals were recruited in Shanxi Eye Hospital from April 2013 to September 2014, including 20 patients with vernal keratoconjunctivitis (VKC), 20 patients with seasonal allergic conjunctivitis (SAC), 20 patients with perennial allergic conjunctivitis (PAC) and 20 normal healthy subjects.Surficial tissues were binocularly scraped using disinfected scraper from upper eyelid conjunctiva, and 4 μl of tear fluid was obtained with capillary tube.The expressions of IL-4, IL-5 and IL-13 protein and mRNA in the conjunctival epithelial cells were detected by immunohistochemistry and real-time fluorescence quantitative PCR.The IL-4, IL-5 and IL-13 concentrations in tear fluid were assayed by Luminex method.This study complied with Declaration of Helsinki and the research protocol was approved by the Shanxi Eye Hospital Ethics Committee.Written informed consent was obtained from each subject prior to entering the cohort.Results IL-4, IL-5 and IL-13 were positively expressed in cytoplasm of conjunctival epithelial cells in the VKC group,SAC group and PAC group,but the expressions of IL-4,IL-5 and IL-13 were absent in the normal control group.The relative expression levels of IL-4 mRNA were 4.11±1.24,2.71±0.71 and 2.00±0.80;the relative expression levels of IL-5 mRNA were 4.02±0.43,2.07±0.45 and 1.47±0.50;and the relative expression levels of IL-13 mRNA expression levels were 6.44±0.66,4.35±1.26 and 2.39±0.86 in the VKC group,SAC group and PAC group, showing significant differences among the 4 groups (F =51.32,220.18,162.49, all at P＜0.01).The relative expression levels of IL-4,IL-5 and IL-13 mRNA were significantly higher in the VKC group than those in the SAC group and PAC group;and those in the SAC group were significantly elevated in comparison with the PAC group (all at P＜0.05).No IL-4, IL-5 and IL-13 were detected in the tear fluid in the normal control group;while the concentrations of IL-4,IL-5 and IL-13 in the tear fluid were (14.06±3.50), (10.88±1.82) and (34.28±8.42) pg/ml in the VKC group,and (7.71 ±0.65), (5.10± 1.33), (23.77±6.29) pg/ml in the SAC group as well as (3.30± 1.50) pg/ml, (2.43± 1.28) pg/ml and (17.67 ± 4.28) pg/ml in the PAC group, showing significant differences among the 3 groups (F =200.29,260.49,128.23, all at P＜0.01).IL-4, IL-5 and IL-13 concentrations in the tear fluid were significantly higher in the VKC group than those in the SAC group and PAC group,and those in the SAC group were significantly raised in comparison with the PAC group (all at P＜0.01).Conclusions IL-4,IL-5 and IL-13 participate in the pathogenesis of multiple allergic conjunctivitis,but their expressions in the ocular surficial tissue are discriminatory in different types of allergic conjunctivitis.

AIM: To investigate the refractive distribution and analysis risk factors for aircrew ametropia.
METHODS: The number of 49 cases with ametropia from 1031 aircrew during May 2013 to May 2014 were reviewed. Various types of refraction composition, age, type, position, time of flight with the subjective assessment of aircrew were analyzed and compared.
RESULTS:Of 49 cases, 43 cases (88%) were myopia, 6 cases ( 12%) were hypermetropia. , Detection rates were higher in age over 50 years aircrew and flight time more than 3000h. Detection rates were lower in self-conscious symptom heavy aircrew, fighter aircrew and good habit of using eyes.
CONCLUSION: The myopia incidence in aircrew with age >50 years and long flight time is higher, than that of fighter pilots and good habit of using eyes. We should pay attention to the increasing late-onset myopia of aviators and habit of using eyes, work intensity and time of using eyes about aircrew.

Notoginseng Radix et Rhizoma (Sanqi), the underground part of Panax notoginseng (Burk.) F. H. Chen (Araliaceae) is commonly used in Chinese medicine for treatment of haemorrhage, haemostasis, swelling, etc. The aerial part including leaves, flowers and fruits are also applied for similar functions. Triterpenoid saponins are considered to be responsible for the biological activities of Sanqi. Up to date, more than 100 saponins have been isolated from theroots, rhizomes, leaves, flowers and fruits of P. notoginseng. The reported saponins can be classified into protopanaxadiol (PPD), protopanaxatriol (PPT), C17 side-chain varied and other types, according to the skeletons of the aglycons. The present review summarizes the saponins isolated from P. notoginseng and their distribution in different medicinal organs, as well as the pharmacological actions on cardiovascular system.
Animals ; Cardiovascular System ; drug effects ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Humans ; Molecular Structure ; Panax notoginseng ; chemistry ; Plant Roots ; chemistry ; Saponins ; chemistry ; pharmacology

Objective To investigate the value of blood culture result in guiding clinical rational use of drugs in clinic.Methods The medical records of 100 children patients with blood culture positive in the four departments of our hospital were retrospectively analyzed.Results The empirical treatment accounted for 98%,the goodness of fit in the medication before culture with the appropriate antibacterial drugs according to final detected bacteria was only 51.6%;after issuing the level 1 report,the adding and substituting drugs in adjusting the antibacterial drugs accounted for about 20% each,60% of antibacterial drugs were not adjusted;after issuing the level 2 report,about 5% of children patients added the antibacterial drugs,the proportion for replacing antibacterial drugs reached 32%,meanwhile the proportion for reducing accounted for 3%;after issuing the level 3 report,the consistency of antibacterial drugs use with the final drug susceptibility test results reached 83%,the inconsistency was only about 5%.Conclusion Clinical pediatric doctors in this hospital still heavily rely on the results of blood culture 3 levels reports,executing antibacterial agents from the experience treatment to the target treatment is also very active.

Objective To evaluate the mechanical properties of SEI silicone for maxillofacial prosthesis before and after heat aging and explore its clinical value. Methods Two kinds of silicones, SEI and A-2186, were performed heat aging for 24, 48 and 72 h. The parameters of tear strength, tensile strength, elongation at break and hardness of them were detected under ordinary state and at different time points after heat aging. Statistic comparison and analysis were conducted. Results Tear strength, tensile strength and elongation at break of A-2186 silicone were more favorable than those of SEI silicone under ordinary state, while the parameter of hardness was an exception (P < 0.05). After heat aging and with time progress, tear strength, tensile strength and elongation at break significantly decreased and hardness significantly increased for both A-2186 silicone and SEI silicone (P < 0.05). After heat aging, tear strength and tensile strength of A-2186 silicone were more favorable than those of SEI silicone, while the latter had a minor change in hardness. Conclusion Compared with the worldwide commonly used A-2186 silicone, there is room for improvement in tear strength and tensile strength for SEI silicone. Given the favorable property of minor change in hardness after heat aging, SEI can be used as a prosthetic material for facial soft tissue defect.

Current materials used for maxillofacial prostheses are far from ideal,and how to develop a final prosthetics material is a significant topic in the research of maxillofacial prostheses.In this paper,some pertinent research of silicone elastomeric materials,such as mechanical properties and colorant are reviewed.

Aged ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Male ; Middle Aged ; Phytotherapy ; Silicosis ; drug therapy ; Treatment Outcome