To study the pathcytological mechanism of Eales disease and to provide a histological testimony for an effictive therapy.Methods45 cases(48 eyes) of Eales disease that were diagnosed according to vitreous hemorrhage and proliferative vitreoretinopathy or secondary retinal detachment.Cytomorphological examination was taken and vitreous extracts were examined by immunohistochemical staining.ResultLymphocytes and fibroblastes were most of those cells in the vitreous extracts.Immunohistology and electron microscopy revealed that lymphocytes were 78.8% or 61.5% in all specimens,respectively.ConclusionProliferative vitreoretinopathy induced by Eales disease closely relates with lymphocytes.The results suggested that autoimmunity might play an important role in the pathogenic mechanism of the disease

Objective To observe the effect of sinomenine on the proliferation of fibroblast-like synoviocytes (FLS) and expression level of MMP-3 secreted by FLS in rheumatoid arthritis in vitro. Methods FLS were obtained by digesting synovial tissues with collagens and were divided into four groups:sinomenine high, middle, low concentration and control group. The proliferation of FLS was assessed by methyl- thiazolyl-tetrazolium (MTT) assay and the mRNA expression of MMP-3 was measured by semi-quantitative RT-PCR respectively after treated by sinomenine. Results The rate of FLS proliferation was significantly reduced in groups treated by sinomenine. Compared with control group, the mRNA expression of MMP-3 markly decreased in sinomenine groups (P

Objective To investigate the effects of icariin on the expressions of osteoprotegerin(OPG)and receptor activator of nuclear factor-kB ligand(RANKL)mRNA in rat osteoblasts cultured in vitro.Methods Calvarial osteoblasts were obtained from newborn SD rats aged＜24 h by trypsin-collagenase digestion method. After treatment with different concentrations of icariin(10~(-10)-10~(-4) mol/L)for 48 h,total RNA was isolated from osteoblasts.Expressions of OPG and RANKL mRNA were detected by semiquantitative RT-PCR.Results Icariin remarkably increased OPG mRNA levels and decreased RANKL mRNA levels in calvarial osteoblasts in a dose- dependent fasion with a maximum effect at a concentration of 10~(-7)mol/L(P＜0.05).Osteoblasts pre-treated with icariin prevented the inhibitory effect of dexamethasone on OPG mRNA(0.570?0.093 vs 1.083?0.081)and the stimulative effect on RANKL mRNA(1.079?0.050 vs 0.718?0.082)(both P＜0.05).Conclusion Icariin upregulates OPG expression and downregulates RANKL expression in osteoblasts,which may explain the beneficial role of icariin in preventing and treating osteoporosis.

AIM:To evaluate the regulatory effect of microRNA-132 ( miR - 132 ) in human umbilical vein endothelial cell ( HUVEC) .
●METHODS: ln vitro cultured human umbilical vein endothelia cells in hypoxic environment for 6h, then maintained under normal oxygen condition for 3h, 6h, 12h, 24h. miR-132 and peroxisome-proliferator-activated receptor-γ coactivator-1α ( PGC-1α) expression was detected by quantitative Real - time polymerase chain reaction and Western blot analysis. Human umbilical vein endothelial cells transfected miR-132 mimic and miR-132 inhibitor( anti-miR-132 ) were measured by quantitative Real-time polymerase chain reaction and Western blot.
●RESULTS: miR - 132 and PGC - 1α expression was significantly ( P < 0. 01 ) upregulated in the hypoxic environment of cells at 3h compared with the normal oxygen condition. After cells transfection, the hypoxic environment the miR-132 and PGC-1α expression were markedly increased compared with the normal oxygen condition. The cells transfected miR- 132- mimic, the expression of the miR-132 and PGC-1α were higher than that of transfected anti-miR-132 and contrast group ( P<0. 01).
●CONCLUSION:miR-132 level is highly expressed in the HUVEC under hypoxia and may be an effect of regulation for PGC-1α.

Objective To explore the clinical significance of serum clara cell secretory protein(CC16),total immunoglobulin E(TIgE)and eosinophil cationic protein(ECP)in children with asthma.Methods Serum were collected from 59 cases during asthmatic acute attacks,29 asthmatic children who were in mild conditions,and 30 cases who were in moderate to severe conditions,and 30 healthy children.Serum CC16 concentration were measured by enzyme-linked immunosorbent assay(ELISA),TIgE and ECP concentration were measured by uniCAP100.Results The levels of CC16 in serum of asthmatic children during acute attacks were significantly lower than that in control group(t=2.93 Pa

Objective: To explore the correlations between mental health and coping styles and posttraumatic stress response of medical students. Methods: With Symptom Self-rating Scale of Posttraumatic Stress Disorder, Symptom Checklist 90(SCL-90), and Coping Styles Questionnaire, 405 medical students were assessed. Results: The scores of a the factors of SCL-90 were higher in the posttraumatic stress response group than those in the control group, and they had significantly difference (P

Medicine, Chinese Traditional ; Mind-Body Relations, Metaphysical

The special environmental features of high altitude, such as hypobaric hypoxia, low temperature, arid, high solar radiation, variable climate and geochemical anomaly, cause great effects on human physiology and health. It will provide valuable references and new ideas to study drug's metabolism in special environment of high altitude hypoxia, and give the guidance to clinical reasonable medication, avoiding adverse reactions and personalized medicine in plateau areas. This article reviewed the effect of high altitude hypoxia on drug metabolism, elaborated metabolic characteristics of some drugs and the activity and expression of drug metabolism enzymes under hypoxia environment at high altitude, and discussed related mechanism.
Altitude ; Climate ; Cold Temperature ; Humans ; Hypoxia ; Pharmaceutical Preparations ; metabolism

Objective:To analyse the salivary cortisol changes in patients with temporomandibular disorders(TMD)after electrical stimulation,and the relationship between cortisol and pain catastrophizing.Methods:1 1 TMD and 1 1 healthy subjects were evoked by electrical stimulation.The saliva samples were collested before test,50 s and 1 00 s of stimulation after test seperately.Salivary cortisol was analysed by ELISA kit.The pain catastrophizing scale(PCS)was answered by all the subjects before stimulation.Data were statis-tically analysed.Results:The salivary cortisol concentration in TMD patients was higher than that in the controls(P <0.05).After stimulation,it showed a downward trend.No interaction between time and groups.The total scores of PCS including three indexes(ru-mination,magnification,helpless)were positively correlated with salivary cortisol concentration.Conclusion:The unpredictable stress causes high level of cortisol.which is related to pain catastrophizing.

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