Objective To observe the characteristics of the images of optical coherence tomography (OCT) performed on the patients with macular edema, and investigate relationship between the retinal thickness at the central fovea and the best-corrected visual acuity. Methods Fourty-seven patients (54 eyes) with macular edema diagnosed by direct and indirect ophthalmoscopy, three mirror contact lens, or fundus fluorescein angiography (FFA) underwent OCT which was also performed on 50 healthy individuals as the control. The examination focused on the horizontal and vertical planes crossing the central fovea to measure the thickness of the fovea. The correlation between retinal thickness at the central fovea and best-corrected visual acuity was analyzed, and the images of OCT in the patients with macular edema were classified according to the macular configuration. Results Significant difference of the macular configuration and best-corrected visual acuity was found between the control and macular edema group. Three characteristics were found in the images of OCT in the patients with macular edema: sponge-like retinal swelling in 20 eyes (37.1%), macular cystoid edema in 26 eyes (48.1%), and serous retinal detachment in 8 eyes (14.8%). The statistical analysis showed that there was a negative correlation between the thickness at the central fovea and best-corrected visual acuity of affected eyes (r=-0.569, P=0.000). Conclusions The images of OCT in macular edema include 3 types: sponge-like retinal swelling, macular cystoid edema, and serous retinal detachment. The retinal thickness at the central fovea of the eyes with macular edema was thicker than that of the normal ones, and the thicker the fovea is, the poorer the visual acuity will be.

Tis study discusses the applicable relationship between Buyang-huanwu decoction and hemodynamic cerebral infarction from pathogenesis of Qi asthenia causing blood stasis.pathological features of hemodynamic cerebral infarction and the function and mechanism of Buyang-huanwu decoction and so on.It also investigates the methods of applying Buyang-huanwu decoction to treat hemodynamic cerebral infarction from such aspects as dose,occasion,contraindication and attentions.

Objective To explore the effect of single and multiple course dexamethasone on brain development in preterm infants.Met-hods One hundred and eighteen preterm infants delivered after 28-34 weeks′ gestation from Aug.2005 to Mar.2007 in our NICU were enrolled in this study.These infants were divided into 3 groups by antenatalcourses of dexamethasone: control group,single-course group and multi-course group.Supportive treatments were given to all 3 groups.Neonatal behavioral neurological assessment(NBNA) was conducted on expected date of delivery.Mental and psychomotor developmental index was evaluated at 3,6,12 months by using intellectual development table made at Children′s Development Center of China(CDCC).Results The score of NBNA was much higher in single-course group than that in multi-course group and control group(Pa

To explore the feasibility of chemical and biological method in evaluation of the in vitro dissolution rate of Liuwei Wuling tablet (LWT), this experiment investigated the inhibitory effect of LWT dissolving solutions on LX-2 hepatic stellate cells in 0.1% SDS dissolution medium in different dissolving periods. From these results, the cumulative dissolution rate of LWT was obtained based on the cell inhibitory rate. The dissolution rates of deoxyschizandrin, phillyrin, and Specnuezhenide were determined by HPLC method. A novel approach of self-defined weighting coefficient had been created to establish the integrated dissolution rate model. Then f2 similar factor method was used to evaluate the relevance of these two methods. The results showed that f2 values for deoxyschizandrin, phillyrin, Specnuezhenide, and the integrated dissolution were 61, 43, 61 and 75 respectively, indicating that the dissolution of multi-component integration could fully reflect the biological potency of the whole recipe. The dissolution evaluation method for multicomponent integration based on biological activity is expected to be one of the effective means for in vitro dissolution test of LWT.
Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; chemistry ; Kinetics ; Quality Control ; Solubility ; Tablets ; chemistry

Objective To synthesize a novel polymer POMs-CS/nano-HAP and study its biocompatibility and safety.Methods The biocompatibility was evaluated through the experiments including acute systemic toxicity test,pyrogen,intracutaneous test.Results The crystals of POMs-CS/nano-HAP were equal on sizes, which average diameter was 20 nm and length was 100 nm.By comparing,the polymer were better than those reported,which were closer to the natural bone.The material had no toxicity,no irritation to skin.(Conclusion The) POMs-CS/nano-HAP artificial bone possesses excellent biocompatibility.

Animals ; Herpesviridae ; genetics ; metabolism ; Herpesviridae Infections ; virology ; Humans ; Viral Envelope Proteins ; genetics ; metabolism

AIM To research the therapeutic effects of Hanbi Formula (Astragali Radix,Aconiti Radix cocta,Scorpio,Scolopendrap and Pheretima) on adjuvant-induced arthritis rats (RA) and its mechanism of action.METHODS RA rat models were established by using Freund's adjuvant,and then the rats were divided into six groups,namely control group,model group,dexamethasone positive group,Baoguang Fengshi Liquid (Notopterygii Rhizoma et Radix,Radix angelicae pubescentis,Chuanxiong Rhizoma,etc.) positive group,and low,high doses of Hanbi Formula groups.The volume and swelling of toes were measured.The interleukin-1 β (IL-1β) and tumor necrosis factor-α (TNF-α) of serum were detected by ELISA;the proliferative capacity of lymphocytes was tested by methyl thiazolyl tetrazolium (MTI) method;synovial tissue was histopathologically examined with HE staining.Finally,the expressions of interleukin-17 (IL-17) and TNF-α in synovial tissue were determined by immunohistochemical assays.RESULTS Hanbi Formula could significantly relieve toe swelling of RA rats.Compared with the model group,Hanbi Formula could significantly alleviate synovitis in rats with RA,down-regulate the expressins of IL-1 β and TNF-α in serum and synovial tissue,and inhibit lymphocyte proliferation.There were no significant differences in above indices between low-dose and high-dose Hanbi Formula groups,which was quite with Baoguang Fengshi Liquid,but less than dexamethasone.CONCLUSION Hanbi Formula possesses an obvious function of anti-RA,and its mechanism may be related to the inhibition of lymphocyte proliferation and reducing secretion of inflammatory cytokines.

Injectable bone tissue engineering scaffold material has a certain form and mechanical strength.It can be loaded with seed cells for injection to the defected site of bone tissue in the form of fluid.After that,structural recovery and functional reconstruction are achieved,and the new bone is formed ultimately.This material has the characteristics of small trauma and good plasticity.It can repair the irregular bone defects and well associate with growth factor,which is the ideal method for repairing bone tissue defects up to now.Among the various injectable bone tissue engineering materials,bioceramic materials,polymer materials,etc.have been proved to have a high biological compatibility and good mechanical properties.That makes these materials become the focus of current research in bone tissue engineering materials.In this paper,the development and application of bioceramic materials,polymer materials,bioceramics and polymer composites were reviewed.

Aim To investigate the effects of cell pro-liferation and activation in HSC-T6 cells by inhibiting the expression of EZH2 , and its partial relevant mech-anism. Methods By introducing the inhibitor DZNep in activated HSC-T6 cells stimulated by TGF-β1 , the protein expression levels of EZH2, p-ERK, p-AKT andα-SMA were detected by Western blot. The siRNA targeting EZH2 was designed and synthesized according to its nucleotide sequence, and their corresponding ex-pression vectors were constructed and transfected into HSC-T6 cells with LipofectamineTM 2000. The prolifer-ation of HSC-T6 cells was determined by MTT. And the protein expression levels of EZH2, p-ERK, p-AKT and α-SMA were measured by Western blot. Results By introducing the inhibitor DZNep in activated HSC-T6 cells stimulated by TGF-β1 , it effectively de-creased the protein levels of EZH2 and also the protein levels of p-ERK, p-AKT and α-SMA. By introducing EZH2-siRNA in activated HSC-T6 cells, it effectively inhibited the cell proliferation, and also the protein levels of EZH2, p-ERK, p-AKT andα-SMA. Conclu-sion Silencing EZH2 expression inhibits HSC-T6 cell proliferation and activation, and EZH2 may be a poten-tial therapeutic target gene for hepatic fibrosis.

Objective To label rat bone marrow mesenchymal stem cells (BMSCs) with superparamagnetic iron oxide in vitro, and to monitor the survivorship and location of the labeled BMSCs in rat models of traumatic brain injury (TBI) with high field MR. Methods BMSCs were cultivated in vitro, and were labeled with SPIO. TBI models were built in the left hemisphere of the rats with Feeney's method. Then SPIO-labeled BMSCs were grafted stereotactically into the region nearby the contusion site 24 h later. The rats underwent MR examination 1 day, 3 days, 1 week and 3 weeks after implantation. Results Brown iron particles could be demonstrated in the SPIO-labeled BMSCs under inverted phase contrast microscope. Numerous intracytoplastic iron particles were stained with Prussian blue, and diffused distribution of iron particles could be seen in the intracytoplasm under electron microscope. At implanted sites low signal intensity could be observed on every sequence of MR examination, among which T2~*WI and SWI were better than other sequences, and SWI was the best. Conclusion MR is sensitive of tracking the survivorship and location of the labeled BMSCs, and SWI is the most sensitive sequence to detect the labeled cells.