Objective To investigate the effects of cinnamic acid to enhance the exercise endurance in mice .Methods Through the exhaustive swimming to establish mice exhaustive exercise model ,the mice were divided into control group ,exercise control group and exercise cinnamic group(n=10) ,the mice exhaustive swimming time were comparted ,blood lactic acid(LAC) lev‐els were used to explore the endurance of cinnamic acid in mice;the super oxide dismutase (SOD) ,malondialdehyde (MDA) and peroxidase (CAT) content in liver homogenates were used to detect mice endurance enhancement and antioxidant capacity of cin‐namic acid ,and expression of Na+ /K+‐ATP enzyme ,Ca2+ /Mg2+‐ATP enzyme on mice quadriceps by Western blot to further study its effect on improving exercise capacity in mice .Results Cinnamic acid significantly prolonged the swimming time of mice;Exercise cinnamic group compared with control group:although the LAC content in plasma elevated ,the difference was not statistically sig‐nificant(P>0 .05) ,SOD and CAT in liver homogenate was significantly decreased(P< 0 .05) ,MDA increased significantly(P<0 .01) ,CAT was decreased but with no statistically significant(P>0 .05) ,the expression level of Na+ /K+‐ATP enzyme and Ca2+ /Mg2+‐ATP enzyme decreased significantly (P< 0 .01) .Exercise control group compared with control group:the LAC content in plasma increased significantly(P<0 .01) ,SOD and CAT in liver homogenate was significantly decreased(P<0 .01) ,MDA increased significantly(P< 0 .01) ,expression level of Na+ /K+‐ATP enzyme and Ca2+ /enzyme Mg2+‐ATP decreased significantly (P<0 .01);Exercise cinnamic group exercise control group:the LAC content in plasma was significantly reduced(P<0 .01) ,SOD and CAT in liver tissue significantly increased(P< 0 .01) ,MDA decreased significantly(P<0 .01) ,CAT decreased significantly(P<0 .01) ,the expression level of Na+ /K+‐ATP enzyme and Ca2+ /Mg2+‐ATP enzyme decreased significantly(P<0 .05) .Conclusion Cinnamic acid has good oxidation resistances and anti‐fatigue ability .

Chronic Disease ; Consensus ; Heart Failure ; diagnosis ; therapy ; Humans ; Integrative Medicine

Objective To investigate the correlation between vitamin D receptor gene and bone mass and obesity phenotypes in patients with type 2 diabetes mellitus.Methods 318 patients with type 2 diabetes were chosen as diabetes group,and 50 healthy people were selected as healthy control group.Vitamin D receptor gene Apa Ⅰ type was detected in the two groups.Height,weight and body mass index(BMI)biochemical index,fat content(FM),lean tissue content(LM)and bone mineral density were detected in patients with type 2 diabetes mellitus.The relationship between vitamin D receptor gene(Apa Ⅰ)polymorphism and BMD and obesity phenotypes in type 2 diabetes was analyzed.Results The VDR gene distribution between the diabetes group and healthy control group showed no signif-icant difference(Z =0.561,P >0.05).The vitamin D receptor genotype in the diabetes group included AA 31 cases (9.7%),Aa type 108 cases(34.0%),aa type 179 cases(56.3%),while the vitamin D receptor genotype in the healthy control group comprised AA 7 cases(9.3%),Aa type 29 cases(38.7%),aa type 39 cases(52.0%).The percentage of AA in both groups was significantly less than that of Aa and aa(χ2 diabetic group =4.127,3.976,all P <0.05;χ2 healthy control group =5.129,4.213,all P <0.05).Proportion of normal bone mass and average bone density in AA,Aa,aa type decreased(χ2 =15.552,P <0.05;F =5.127,P <0.05),the genotype AA was not detec-ted in osteoporosis group.BMI and FM were the highest in AA,which were significantly higher than those of Aa,aa (F =4.319,4.263,all P <0.05).Conclusion Vitamin D receptor gene Apa Ⅰ type polymorphism is related with BMD and obesity in type 2 diabetes mellitus,and it has predictive value on bone mass changes.The increase of BMI and FMmay be beneficial to bone mineral density.

Objective To investigate the protective effect and it's possible mechanism of Tanshinone ⅡA in radiation-induced pulmonary fibrosis.Methods Having the right hemithorax of female Wistar rats irradiated(30?Gy) in 10 fractions within 14 days by 6 ?MV photons,the radiation-induced pulmonary fibrosis animal model was established.In the treatment group,sodium Tanshinone ⅡA sulfonate(15?mg/kg) was given by intraperitoneal injection 1 hour before each fraction of irradiation.Five months after irradiation,the difference of the histopathological changes,the hydroxyproline content and expression of TGF-?1 between the radiation alone group,tanshinone plus radiation and control group were analyzed by HE stain,Massion stain,immunohistochemical methor and reverse transcriptase polymerase chain reaction(RT-PCR) method.Results The histopathological comparison revealed the protective effect of Tanshinone ⅡA.The content of hydroxyproline was(21.99?3.96),((38.25?)(7.18)),(28.94?4.29)??g/g in the control group,radiation alone group and radiation plus Tanshinone ⅡA.The expression of TGF-?1(mRNA and protein) was reduced by Tanshinone ⅡA.Pathological changes of the pulmonary fibrosis was reduced by Tanshinone ⅡA yet.Conclusions Our study shows that Tanshinone ⅡA can inhibit radiation-induced pulmonary fibrosis,and the possible mechanism of its may be made possible through down-regulating the expression of TGF-?1 in the irritated lung tissue.

Objective To investigate the changes of monocyte chemoattractant protein-1(MCP-1)in serum and urine of lupus nephritis(LN)patients in active phase and remission phase.Methods The levels of MCP-1 in serum and urine of 58 LN patients(27 of active phase and 31 of remission phase)were measured by ELISA.The correlation between the levels of MCP-1 in variant phase of LN and other relevant factors were analyzed.Results The MCP-1 levels in sera of both active phase and remission phase of LN patients were markedly higher than those in controls(548.5?347.2 ng/L and 469.1?298.4 ng/L vs 273.3?146.7 ng/L,P0.05).Conclusion The MCP-1 levels in urine of LN patients is more suitable to evaluate the activity of disease as a sensitive marker.

Based on the introduction and analysis about PLoS One, and its number of published papers, impact fac-tor, publishing period, manuscript employed, publication fee, auditing, requirement for paper etc. , this paper pointed out break the barriers of traditional publishing model from several aspects, create a new way of open access scholarly pub-lishing, promoted the fast communication of scientific research. As the author of China, can choose more PLoS One pub-lished as our scientific research platform, improve our position in the field of scientific research in the world.

Objective To summarize the experience of modified mini-cholecystectomy. Methods A total of 128 patients underwent mini-cholecystectomy by using a modified Bookwalter retractor, a self-made deep-site tying tool and an L-shape electrotome from September 1998 to June 2003. Results That the length of incision ≤ 6 cm was regarded as a surgical success. Out of the 128 patients, the surgical success was achieved in 110 patients (85 9%). Conclusions In mini-cholecystectomy, the use of the modified Bookwalter retractor greatly improves the visualization of operation field, and the use of the self-made tying tool facilitates deep-site manual knot tying.

Objective To evaluate the relationship between fragile histidine triad(FHIT) transcription abnormalities and HPV16 infection and human cervical tumorigenesis.Methods Total RNA from 5 cervical carcinoma cell lines(SiHa,HeLa,RJC-1,CS1213 and C4-1),58 primary cervical cancer specimens and 18 normal cervical epithelial tissues were extracted and FHIT transcripts were characterized by a two-step(nested) reverse transcription(RT)-PCR.The seven of the PCR products with different size were purified and sequenced.HPV16 infection was assessed by PCR.Results ① There were altered FHIT transcripts in SiHa,HeLa and C4-1 cells.Aberrant FHIT transcripts were detected in 39 out of the 58 cervical cancer samples(67.2%),but none out of 18 in the normal cervix tissue specimens(0%);HPV16 infections were identified in 37 of the 58 cervical cancer tissues(63.8%),but 1 in the 18 normal cervical epithelial tissues(5%),which showed a significant difference between these two groups(P0.05).③ The exon 5 and exon 6 were mainly deleted in the altered FHIT transcripts and no insertion and point mutation were found by DNA sequencing.Conclusion Aberrant FHIT expression was significantly common in cervical cancers and was correlated with HPV16 infection.These findings suggest that the tumor suppressor gene FHIT and high risk HPV16 may play a very important role in human cervical carcinogenesis.

Objective To study effects of Shoucong capsule on the learning-memorial ability,CAT and MAO activities in brain tissue of AD rat.Methods Male Wistar rats were randomly divided into 5 group:blank compared group,model compared group,Naofukang group and Shoucong capsule(low,high dose) group,every group contained 9 rats.They were injected with D-galactose into the neck and injected with scopolamine into the intraperiton to making AD compound model rats.The learning-memorial ability were examined by diving-stand test,and CAT and MAO activities in brain tissue were determined by spectrophotometry.Results Shoucong capsule greatly deceased error time and enhanced the electric latent period,and enhanced the activities of CAT while deceased the activities of MAO in model rats.Conclusions Shoucong capsule has significantly effect of preventing and treating senile dementia in AD rats by enhancing the activities of CAT and deceasing the activities of MAO in brain tissue.

The purpose of this study was to investigate clinical feasibility of treatment of pancreatic pseudocyst by endoscopy. We reported here one case of large pancreatic psuedocyst that was treated by endoscopic ultrasonography guided cystogastrostomy.The patient's symptoms resolved on the same day of operation. And one week later, the pancreatic pseudocyst decreased by more than 50% in size. Pancreatic psuedocyst resolved and didn't relapse after 6 months. So endoscopic ultrasonography guided cystogastrostomy is a safe, reliable, effective therapy for pancreatic pseudocyst with few complications.