Objective: Recent studies have shown that cell implantation can replace infarcted myocardium to improve cardiac performance. The present study was designed to investigate the effects of autologous bone marrow mononuclear cell (BM-MNC) implantation into myocardium bordering the infarction with or without induced by 5-azacytidine on cardiac function after acute myocardial infarction (AMI) in rabbits. Methods: AMI was replicated by ligating the left anterior descending coronary artery. Rabbits were randomly divided into three groups: (1) BM -MNC induced by 5-azacytidine implantation (n=7), (2) BM-MNC implantation alo ne (n=7), and (3) AMI control (n=7). In addition, sham-operated (n=5) rabbit s were randomly selected to serve as non-infarction control. Animals for cell im p lantation were received intramyocardial injection of autologous BM-MNC in myoca rdium bordering the infarction, and echocardiography and hemodynamic studies wer e performed to evaluate cardiac function following 28 days of implantation. Results: Compared with the sham-operated group, the left ventricle (LV) end diastolic pressure (LVEDP) was significantly increased (P0.05). Conclusion: BM-MNC induced by 5-azacytidine implantation into myocardium bordering the infarction can significantly improve impaired cardiac function associated with LV remodeling after AMI, however such improvement is not further promoted compared with that in BM-MNC implantation group alone.

Objective The incidence of pancreatic cancer appears to be increasing and the prognosis is poor. Recent studies have revealed that angiotensin Ⅱ type 1 receptor (AT1) stimulates the growth and angiogenesis of tumor, and selective AT1 antagonist inhibits these effects. This study is investigated the expression of AT1 in pancreatic cancer cell lines. Methods The pancreatic cancer cell lines used in this study were SW1990, PaTu8988s and PANC-1. RT-PCR was used to detect the AT1 mRNA expression, and ABC immunocellularchemical staining and sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) were used to detect the AT1 protein expression. Results Both AT1 mRNA and protein were expressed in the three cell lines. The AT1 protein was found in the cell membrane and cytoplasm of these cells. The AT1 protein ( M r 44?10 3) was also demonstrated by SDS-PAGE. Conclusion The results suggest AT1 may play an important role in pancreatic cancer growth, and inhibition of AT1 might be a useful therapeutic strategy.

Child ; Hemofiltration ; Humans ; Male ; Paraquat ; poisoning ; Poisoning ; therapy ; Treatment Outcome

Analysis of setbacks found in the existing system and mechanism of public hospitals. Based on the health reform principles of Separation of administration and management, Separation of administration and operation, Separation of prescribing and dispensing, and Separation of for-profit hospitals and nonprofit hospitals, the authors named the following roadmap for public hospital governance reform. First, set up a management committee for each public hospital to coordinate the government function for medical service; set up a public hospital authority to upgrade the management level of these hospitals; transform functionality of health authorities to encourage diversity in medical service providers. Second, establish the tiered decision making mechanism, autonomous operation system and diversified supervision system, to separate administration and management. Third, formulate articles of association for hospitals to encourage their evolution to legal entities, consolidating the government reforms by legal means.

Objective To investigate the relationship between the expression of tumor apoptosis inhibitory protein(survivin) and the apoptosis induced by arsenic trioxide(As2O3) in transcatheter arterial chemoembolization therapy.Methods Sixteen Japanese big-ear white rabbits with implanted hepatic VX2 tumor at both right and left hepatic lobes were randomly and equally divided into two groups.Three weeks after the tumor was inoculated,1 ml lipiodol(UFLP) and 2 mg As2O3 were injected via hepatic arterial cannulation into the rabbits of study group,while only 1 ml UFLP was used for the rabbits in control group.Three weeks later,all the rabbits were sacrificed,and the tumor tissue,the tumor-neighboring tissue and the normal liver were separately collected and sent for TUNEL staining and examinations,which included the observation of apoptosis of tumor cells and the assessment of the expression of survivin protein.Results In study group,a large number of yellow apoptosis cells was seen in the tumor tissue but no apoptosis cell was found in the tumor-neighboring tissue or in the normal liver tissue.In the control group,no yellow apoptosis cell was observed in the tumor tissue,tumor-neighboring tissue or normal liver tissue.The survivin protein expression rate of the tumor tissue was 100%(16 / 16) in the control group,including strongly-positive in 12 and weakly-positive in 4 rabbits.In contrast,the survivin protein expression rate of both the tumor-neighboring tissue and the normal tissue was 0%.In study group,the survivin protein expression rate of the tumor tissue was 37.5%(6 / 16),including strongly-positive in 2 and weakly-positive in 4 cases,and the survivin protein expression rate of both the tumor-neighboring tissue and the normal tissue was 0%.Significant difference in survivin protein expression rate of the tumor tissue existed between two groups(P

Objective:To determine the pharmacokinetics of continuously infused midazolam in patients during intensive care. Method: The study involved six critically ill patients who required sedation to facilitate controlled ventilatory support. The infusion rate of midazolam was adjusted individually to achieve an appropriate level of sedation. Blood samples were taken during and after the continuous infusion of midazolam to measure plasma concentration of midazolain with high performance liquid chromatography,and pharmacokinetic parameters were calculated. Result:The mean value of elimination half-life was 5.09 h(range: 3.19-7.30h), and the volume of distribution was 64.45L(range: 10.41-135.25L). The clearance was 7.29L/h, Conclusion:Clinical pharmacokinetics of midazolam in critically ill patients is highly variable. The increased volume of distribution in the patients is the major determinant for the slower elimination of midazolam.

Objective: To investigate the effects of pentoxifylline (PTX) on oxygen free radicals induced myocardial injury. Method: An in vivo myocardial impairment model was developed in SD rat with exogenous oxygen free radicals. The changes cardiac performance and serum malonylaldehyde (MDA), lactic acid, cretine phosphokinase (CK) levels were tested before and at different interval after I. V. administration of oxygen free redieal. Result: Myocardial performance after oxygen free radicals administration was significantly depressed compared to the baseline values (P

Objective To evaluate the efficacy of PTX on the global cerebral ischemia reperfusion injuryMethods The global cerebral ischemia of gerbils was induced by clamping bilateral carotid arteries for 30 min , then declamping with the reperfusion lasting 90 min At the beginning of ischemia , PTX 25mg?kg -1 or 50mg?kg -1 was administered intravenously by mini infusion pump In another group , PTX 25mg?kg -1 was infused at the beginning of reperfusion In sham operated and control groups the same volume of 0 9% NaCl were infused 0 2% Even's blue 1ml?100g -1 was injected intraperitoneally At the end of experiment , The gerbils were decapitated to be preserved at -70℃ for measurement of water contents of whole hemispheres , cerebral superoxide dimutase (SOD) activity, malonyldialdehyde (MDA) content and Even's blue content Results As compared with those in control group, at the beginning of ischemia or reperfusion ,the administration with PTX 25mg?kg -1 significantly reduced the cerebral water content (P

OBJECTIVE:To promote the information management of Chinese medicinal decoction piece.METHODS:Information management modes in every part of the management of Chinese medicinal decoction piece were discussed basing on network structure and operating environment of hospital drug management system.RESULTS&CONCLUSION:Database for rational use of Chinese medicinal decoction pieces has been established to provide links of network operation platform and rational processes,which are beneficial to information management of Chinese medicinal decoction piece.

3 months) showed that 47. 2% of 36 patients had persistent relief without pain recurrence. Besides pain relief, the interventional endoscopy resulted in weight gain and improvement in quality of life in 63. 9% of 36 patients. Furthermore, the incidences of hyperamylasemia and bleeding which recovered after medication only within 3 days were 13. 9% and 5. 6% , respectively. Stent occlusion and detachment occurred respectively in 12. 5% and 4. 2% of 24 patients undergoing stent placement. Conclusion Endoscopic therapy for patients with pancreatic duct stricture appears to be safe and effective.