Objective]To sum up director He Ruoping's feature in recognizing ovarian cancer causa morbi and mechanism and clinical treatment rules; To explore the clinical experience of director He Ruoping treatment with ovarian cancer.[Method]By learning from director doctor He Ruoping for three years and combining my own clinical experience, from the etiology and pat hogenesis, it sums up the teacher He' s unique feature of by stages treatment of ovarian cancer, and with one case for detailed explanation.[Result]Teacher He advocates the combination of traditional Chinese medicine and western medicine, puts forward the principle of ovarian cancer.By the way of strengthening body resistance and eliminating evil, Teacher He adopts the help of invigorating the spleen and tonifying the kidney,supplementing qi and nourishing yin,soothing the liver and regulating qi,clearing away heat and toxic material,promoting blood circulation to remove blood stasis, softening firm scattered nodes,and water permeability wet for the main therapy in treatment of ovarian cancer and the curative effect is remarkable.[Conclusion] To take Teacher He' s experience for treating ovarian canceR from essence,replenishing qi and blood,removing turbidity can significantly relieve patients' clinical symptoms and improve patients' life quality.

Objective To investigate the antibacterial activity of the alkaloids extract from Oxytropis falcata Bunge against 9 common pathogens in vitro. Methods Alkaloids was extracted from Oxytropis falcata Bunge. The agar plate dilution method was adopted to evaluate the activity against bacteria of 4 Gram-negative test strains and 5 Gram-positive test strains, while the MIC and MBC were determined by tube double dilution method. Results The alkaloids extract from Oxytropis falcata Bunge had the antibacterial activity against 9 pathogens and there was a good dose-response relationship between sensitivity and concentration. Antibacterial activities against bacillus subtilis and stentrophmomas maltophilia showed stronger than other tested bacteria, the MIC and MBC were 0.95 mg/mL and 1.91 mg/mL, respectively. In other tested bacteria, MIC and MBC were ranged in 1.91-7.63 mg/mL. Conclusion The alkaloids extract showed a broad spectrum of a stronger antibacterial activity against all tested pathogens. Gram-positive strains were more sensitive than Gram-negative strains to the inhibitory effects of the alkaloids extract.

Persister cells are dormant or slowly grown variations in microbial populations .They are highly tolerant to antibiot-ics but the tolerance are not inherited as the genetic resistance , when persisters are inoculated to fresh medium , most bacteria are still susceptive to antibiotic , while only a small fractiont become persisters again .Persisters are believed to be closely related to clinic bio-film forming and the recurrence and recalcitrance of chronic infections .Different persisters have been found in Escherichia coli , Pseud-omonas aeruginosa , Staphyococcus aureus , Mycobacterium tuberculosis , Candida albicans and so on , and a few mechanisms about per-sisters formation have been studied .This article reviews the current progresses of research methods and achievements on persisters from different levels .

[Objective] To explore the relationship between brittle diabetes and yin fire, so as to more comprehensively understand fragile diabetes from TCM, broaden the clinical diagnosis and treatment, and improve the clinical curative effect. [Methods] The theory of yin-fire theory was summarized, and the relationship between brittle diabetes and yin-fire was expounded from the aspects of academic origin, pathological basis, clinical manifestation and so on, proposed treatment principles and prescriptions.[Result] The yin fire theory originated from Nei Jing. Yin and yang do not rise, wet muddy bets, endogenous fire, the fire is too weak. Fever is the reason for the formation of diabetes, Yin fire is an important reason for fluctuations in blood sugar; brittle diabetes expelling Bu Zhong Yi Qi, lifting fine, Xie Yin fire, from Dongyuan Qingshu Yiqi Decoction to the treatment of fragile diabetes sugar made a good effect. [Conclusion] The theory of yin fire has important practical significance for diabetes, especially brittle diabetes. It is a new idea and method to treat brittle diabetes by the thought and method.

Objective To investigate the effect of ketamine on c-fos gene expression in the glutamate induced injury of neuronal PC12 cells line. Methods The differentiated PC12 cells were seeded in 6-well plates(2?10~6/well) and incubated for 18 h,and then were randomly allocated to receive fresh medium(group C)or(10 mmol/L) glutamate(group G) or 0.1 mmol/L ketamine plus 10 mmol/L glutamate(group K1) or 0.5 mmol/L ketamine plus 10 mmol/L glutamate(group K2) or 1.0 mmol/L ketamine plus 10 mmol/L glutamate(group K3).At 5,15,30,60,120,240 and 360 min after administration of these drugs,the cells were collected respectively.(Total) cellular RNA was extracted.Reverse transcriptase-polymerase chain reaction was applied to determine cDNA amplification products with GAPDH mRNA as an internal control.Densities of DNA bands were quantified using the image analysis system.(Results)c-fos mRNA increased at 15 min,peaked at 30 min and 60 min,decreased at 120 min,reco-(vered) to the base level at 360 min among group G,K1 and K2.The c-fos mRNA levels were markedly elevated in group G as compared with the control levels(P

Objective To investigate the expression of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in hippocampus after status convulsion (SC) and explore the influence of age and duration of SC on the expression of BDNF and NGF. Methods Animal model with the different durations of SC (30 min,3h) were established by intraperitoneal injection of 3 mEq/kg lithium chloride,18-20 h later,followed by 5 mg/kg pilocarpine in 40 adult Wistar rats aged 2-3 months and 40 20-day-old Wistar rats. The normal control and experimental control were made in ten adult rats and ten 20-day-old rats (n=5 for each control). The rats were sacrificed at 3 h,6 h,12 h,1 d,3 d,7 d after 30-minute SC,or 3 h,1 d after 3-hour SC respectively. The location and cell type expression of BDNF and NGF in the hippocampus were observed by immunohistochemistry. The levels of BDNF and NGF at different time points were quantitatively analyzed by enzyme-linked immunosorbent assay (ELISA). Results The cells expressing BDNF and NGF located mainly in the dentate granule cells and CA1-CA4 pyramidal cells of hippocampus. The most positive immunoreactivity was in cytoplasm,partly in axons. The expression of BDNF had the tendency of increase in all rats after 3-hour SC,peaked at 1 d and 3 d respectively. These increases lasted for at least 7 d. Moreover,a several-fold increase was observed at the peak levels. The patterns of NGF expression were similar to that of BDNF after SC. The elevated degree and duration of NGF expression were remarkably lower than that of BDNF,declining abruptly to below control levels by 12 h with the characteristic of biphasic increase. The expression of hippocampal BDNF had the positive correlation with the duration of SC,but that of NGF was not. No matter the duration of SC,there was more rapid and evident induction of BDNF in 20-day-old rats and adult rats. Moreover,the age-related difference was more obvious for the longer duration of SC. Conclusion SC induced the expression of BDNF chiefly in hippocampus,and had minor influence on that of NGF. The expression of BDNF was related to age and duration of SC. There was a more rapid and marked induction of BDNF in 20-day-old rats and adult rats. The age-related difference had the positive correlation with the duration of SC.

The work aims to study the drug metabolizing enzymes involved in the metabolism of butylphthalide and evaluate the induction and inhibition activities of butylphthalide on CYP450 isoenzymes by using in vitro (liver microsome incubation system of rats and human) and in vivo (CYP induced model of rats) method. Butylphthalide was incubated with selective inhibitors of CYP450, and its metabolic rate was determined to identify the metabolizing isoenzymes of NBP in rat (normal and induced rats) and human liver microsomes. The in vitro inhibition effect of butylphthalide on 6 main liver microsomal CYP450 isoenzymes was evaluated by using probe drugs; the induction and inhibition activities in vivo of butylphthalide on CYP450 isoenzymes were evaluated by NBP ig dosing (160 mg x kg(-1)) and iv dosing (20 mg x kg(-1)) in rats. After adding the specific inhibitors of CYP2C11, 2E1 and 3A 1/2 for rat, CYP2C19, 2E1 and 3A4/5 for human, the metabolism of NBP in rat and human liver microsomes were reduced 38.8%, 86.2%, 78.4% and 51.0%, 92.0%, 58.9% of control, respectively. The metabolic rates of NBP in CYP2E1 and 3A 1/2 induced rat liver microsomes were increased 25.5% and 68.9%. High concentration of NBP (≥ 200 μmol x L(-1), in vitro) could inhibit the activities of CYP1A2, 2C6, 2C11 and 2D2 in rats, and high concentration of NBP ( ≥ 15 μmol x L(-1), in vitro) could inhibit the activity of CYP2C19 in human. All the results indicated that NBP should be mainly metabolized by CYP2E1, 2C11 and 3A 1/2 in rats and CYP2E1, 2C19 and 3A4/5 in human. High concentration of NBP could inhibit human CYP2C19 in vitro. No significant induction/inhibition effects of NBP were observed on rat liver CYP450 isoforms after ig 160 mg x kg(-1) NBP or iv 20 mg x kg(-1) NBP.

Objective To analyze the nursing interventions of two kinds of percutaneous nephrolithotomy (PCNL),so that we can provide personalized service for patients with PCNL and improved quality of nursing. Methods 140 cases of patients undergoing PCNL were chosen from January 2010 to January 2012.70 of them received standard PCNL,another 70 cases were given tubeless PCNL.The same nursing method was given before the operation,and personalized nursing was given according to difference in operation method.A self-designed questionnaire was conducted for satisfaction degree with nursing service of all patients on discharge. Results Satisfaction degree with psychological care,catheter nursing and complication nursing of tubeless PCNL was higher than standard PCNL. Conclusions Two methods of PCNL have become conventional therapy for upper urinary calculus,and careful nursing is very important,especially for tubeless PCNL.

Aim To study the therapeutic effect of hesperidin (HDN) on adjuvant arthritis (AA) in rats and its mechanisms.Methods Freund's complete adjuvant (FCA) was used to induce AA in rats. Secondary paw swelling of AA rats was measured with volume meter. Splenic lymphocyte proliferation response induced by concanavalin A (ConA) or lipopolysaccharide (LPS) was examined with MTT assay. IL-2 production of splenic lymphocytes and IL-1, IL-6,TNF-? productions of peritoneal macrophage (PM?) were determined by radio-immunity assay.IL-10 production of PM? was estimated by enzyme linked immunosorbent assay (ELISA).Results The secondary inflammation of AA rats appeared on the 12th day after injection of FCA. At the same time (d 12), HDN (40,80,160 mg?kg-1,?12 d) were given to AA rats by intragastric administration. It was found that HDN(80, 160 mg?kg-1) could significantly inhibit the secondary paw swelling of AA rats from the 20 th day.The suppressed lymphocyte proliferation and IL-2 production of splenic lymphocytes in AA rats were reversed by treatment with HDN. Meanwhile,HDN could remarkably down-regulate IL-1,IL-6,TNF-? productions of PM? and up-regulate IL-10 production of PM?.Conclusions The results suggested that HDN had therapeutical effect on AA rats. Its mechanisms may be related to adjusting abnormal immune function in AA rats and keeping the balance of cytokine network.

Objective To explore the influences of age and duration of status convulsion (SC) on hippocampal neuron apoptosis by observing the dynamic change of neuron apoptosis in rats with different age when SC terminates. Methods Seizures were induced in infant rats (IRs) and adult rats (ARs) injected with lithium and pilocarpine intraperitoneally. Rats were sacrificed at 6 time points (3, 6, 12 hours and 1, 3, 7 days) after 30 minutes of SC, or 2 time points (3 hours and 1 day ) after 3 hours of SC respectively. The location and type of apoptotic cells were assessed by using terminal deoxynucleotidyl dUTP nick end-labeling (TUNEL) of brain sections in situ. The proportion of apoptotic cells was quantified by Annexin-Ⅴ-FITC apoptosis detecting method and analysed by flow cytometer. Results (1) SC induced neuronal apoptosis and necrosis mainly in the CA_1 and CA_3 regions of hippocampus. (2) As compared to the time point before SC, the proportion of apoptotic cells in IRs and ARs hippocampus was increased obviously at 3 hours point after 30 minutes of SC (IRs 0.55%?0.21%, ARs 0.53%?0.06%), and with a maximal induction at 12 hours in IRs (0.67%?0.18%) and 1 day in ARs (0.98%?0.38%). The apoptotic process continued at least for 3—7 days. (3) In IRs, the proportion of apoptotic cells was lower than in ARs at different time points after 30 minutes of SC, except 3 hours point. There was a significant difference between the two age groups at 1 day and 7 days after SC respectively. The age-related difference was more obvious after 3 hours SC. Conclusions (1) Severe seizure induces neuronal apoptosis in hippocampus. (2) Age and duration of SC might be the important factors in influencing the neuronal apoptosis. The neuronal apoptosis in hippocampus after SC would have a positive correlation with age and duration of SC.