OBJECTIVETo probe into a better acupoint association for treatment of hyperplasia of mammary glands and the mechanism.

METHODSTwo hundred cases were randomly divided into a regulating kidney and smoothing liver needling group, a routine acupuncture group and a Chinese medicine group. The regulating kidney and smoot hing liver needling group were treated with acupuncture at Shenshu (BL 23), Mingmen (GV 4), Fengfu (GV 16), Lingxu (KI 24), Shenfeng (KI 23), Bulang (KI 22), Qimen (LR 14), etc. and the routine acupuncture group with acupuncture at Zusanli (ST 36), Tanzhong (CV 17), Ganshu (BL 18), Rugen (ST 18), Neiguan (PC 6), Qimen (LR 14), once daily; and the Chinese medicine group with oral administration of Rupixiao tablet, 6 tablets each time, thrice each day. After they were treated for 2 menstrual cycles, the therapeutic effects and changes of E2, PRL, P, CD3+, CD4+ and CD8+ were observed.

RESULTSThe clinical therapeutic effect and the total effective rate for TCM syndromes were 92.0% and 94.0% in the regulating kidney and smoothing liver needling group, 86.0% and 88.0% in the routine acupuncture group and 78.0% and 84.0% in the Chinese medicine group, with a significant difference among the 3 groups, the former being better than the other two groups; after treatment, serum PRL, P, P/E2 significantly improved in all the 3 groups (P < 0.05) and the regulating kidney and smoothing liver needling group was better than other two groups in decreasing PRL and increasing P, P/E2 (P < 0.05); and E2, CD8+, CD4+/CD8+ significantly improved in the regulating kidney and smoothing liver needling group and the routine acupuncture group, the former being better than the later in decreasing E2 (P < 0.05).

CONCLUSIONThe regulating kidney and smoothing liver needling method is an effective method for treatment of hyperplasia of mammary glands and it has regulative action on endocrine and immune functions in the patient with hyperplasia of mammary glands.

Acupuncture Therapy ; methods ; Adult ; Endocrine Glands ; physiopathology ; Female ; Humans ; Hyperplasia ; Kidney ; physiopathology ; Liver ; physiopathology ; Mammary Glands, Human ; pathology ; Medicine, Chinese Traditional ; T-Lymphocytes ; immunology

It is recognized that prenatal care plays an important role in reducing adverse birth.Chinese pregnant women with medical condition were required to seek additional health care based on the recommended at least 5 times health care visits.This study was to estimate the association between prenatal care utilization (PCU) and preterm birth (PTB),and to investigate if medical conditions during pregnancy modified the association.This population-based case control study sampled women with PTB as cases;one control for each case was randomly selected from women with term births.The Electronic Perinatal Health Care Information System (EPHCIS) and a questionnaire were used for data collection.The PCU was measured by a renewed Prenatal Care Utilization (APNCU) index.Logistic regression models were used to estimate odds ratios (OR) and the 95％ confidence interval (95％ CI).Totally,2393 women with PTBs and 4263 women with term births were collected.In this study,695 (10.5％) women experienced inadequate prenatal care,and 5131 (77.1％) received adequate plus prenatal care.Inadequate PCU was associated with PTB (adjusted OR:1.41,95％ CI:1.32-1.84);the similar positive association was found between adequate plus PCU and PTB.Among women with medical conditions,these associations still existed;but among women without medical conditions,the association between inadequate PCU and PTB disappeared.Our data suggests that women receiving inappropriate PCU are at an increased risk of having PTB,but it does depend on whether the woman has a medical condition during pregnancy.

Canine distemper virus (CDV) neutralizing antibody (NT) titer was examined against the sera from 7 giant pandas aged between 8 to 21 years housed at Chengdu Research Base of Giant Panda Breeding,China.Anti-CDV NT titer against the Onderstepoort strain showed a wide range from × 2 to×256 (median=16),even though the ani-mals had been receiving an attenuated live vaccine made from an anonymous domestic CDV strain twice a year since 2003.A single administration of attenuated morbillivirus antigen often be enough to give corresponding host a steady immunogenicity.Anti-CDV-NT variation in the giant panda suggests some deficiency in the relationship between the vaccine and the host.

OBJECTIVETo determine distribution and mutation pattern of type P ATP7B gene mutation and to explore genotype and phenotype correlation in patients with Wilson's disease (WD).

METHODSSixty patients with WD from 57 no kinship families, 37 male and 23 female, were enrolled in this study. The age of onset ranged from 4.6 - 39 years, < or = 16 years in 55 patients. Some exons of ATP7B gene mutation were analyzed in patients with WD by using biochemical methods, polymerase chain reaction-single strand configuration polymorphism (PCR-SSCP), restriction fragment and DNA sequence analysis. Totally 778 coding regions were identified with restriction enzyme Msp I. The activity of Cu-ATPase was assessed by measuring inorganic phosphorus in 3 patients with known genotype.

RESULTSFifty-two of 60 patients (86%) had presented with hepatic manifestations, 30 of them had only hepatic manifestations, 12/52 patients had hepatic and neurological manifestations at the same time; 10/52 patients had hepatic and other symptom; 7/60 patients had only neurological symptom, one patient had no symptom. Eleven mutations were detected by DNA sequencing, including five missense mutations (R778L, V1140A,G943S, V1106I and V1216M), one deletion (1384del17) and five polymorphisms (IVS4-5T/C, A2495G, C2310G, IVS18 + 6C/T and IVS20 + 5A/G) were identified. R778L mutation was identified 52/114 alleles (45.6%). R778L occurred in 38/52 patients with hepatic manifestation (73%), homozygosis of R778L was demonstrated in 14 patients and heterozygosity of R778L in 24 patients. V1106I mutation was 1.7%, G943S, V1140A, and V1216M was 0.86% respectively in this study. Two patients with delayed onset of neurological symptoms occurred V1106I mutation of ATP7B. Cu-ATPase activity of 3 patients with known mutation (R778L/V1106I, R778L/V1216M and R778L/R778L) declined by 44.55%, 88.23% and 69.49%, respectively, compared with normal control.

CONCLUSIONThe 1384del17bp and V1106I are two novel mutations found in patients with WD. R778L was common mutation of ATP7B gene with frequency of 45.6% in this study. The mutation in exon 8 of WD gene may play an important role in pathogenesis of WD in Chinese. Carriage of R778L mutation seems to be correlated with hepatic manifestation.

Adenosine Triphosphatases ; genetics ; Adolescent ; Adult ; Cation Transport Proteins ; genetics ; Child ; Child, Preschool ; Copper-transporting ATPases ; DNA Mutational Analysis ; Exons ; Female ; Gene Frequency ; Genotype ; Hepatolenticular Degeneration ; enzymology ; genetics ; pathology ; Humans ; Male ; Mutation ; Phenotype ; Polymerase Chain Reaction ; Polymorphism, Single-Stranded Conformational ; Sequence Analysis, DNA