Splice variants are transcripts that exhibit various functions or activities and are obtained from alternative splicing of precursor mRNA. An increasing number of tumor-specific splice variants are being discovered and their roles in cancer development and progression are being investigated. In this study, the expression patterns and activities of tumor-specific splice variants were charac-terized. The results suggest that a variant-specific gene therapy that targets cancer cells would be more specific and would have minimal effect on healthy tissues. Using antisense oligonucleotides, mRNA that contributed to cancer cell survival and altered splicing patterns was downregulated in a clinical trial. The results provide a new appreciation for the gene therapy of cancer. Identification of novel tu-mor-specific splice variants and constant improvement of therapeutic strategies by modifying splicing patterns can lead to the develop-ment of more effective and safer cancer therapies.

Antiviral Agents ; administration & dosage ; therapeutic use ; Hepatitis C, Chronic ; drug therapy ; Humans

0.05).No partial side-effect of the medicine were discorvered in the observation process.Conclusion Long-term inspiration of low-dose GCS to treating the babies and infants with asthma has reliable security.

Breast cancer metastasis is the main cause of tumor death and poor long-term effect in clinical treatment. In the process of metastasis, chemokine may play the main role. In the breast cancer cell, some chemokine and receptor were expressed excessively. It has been shown that through the interaction between chemokine and correspondence receptor, phagocyte and lymphocyte infiltration stimulated, tumor cell invasion into normal tissue with angiogenesis, thus the metastasis was formed. This article reviews the mechanisms of chemokine receptor CXCR4 and chemokine RANTES ,IL-8 MCP-1 in breast cancer metastasis.

Objective To investigate the different effect of positive and negative emotion induced by film clips on explicit and implicit memory,and whether there is mood congruency effect. Methods Thirty participants who were randomly assigned to three experimental groups were investigated. They were exposed in different film clips and then worked on memory task. After each emotional condition,the 5-point Self Assessment Scale was registered , Buchner's processing dissociation corrected model was employed to separate explicit and implicit memory. Results (1) The emotion arousal level of different films is significantly different, the scores of negative film and positive film were more than that of neutral film( (3.24 ± 1.02),(2.85 ± 1.35),(1.12 ± 1.42) , P＜0.01) ; the scores of emotional valence of positive,negative and neutral priming condition were (4. 10 ±0. 20), (3. 60 ± 0.22) and (3.20 ±0. 97), and there was a significant difference between emotion priming conditions (F(2,27) = 46.81, P＜ 0. 01). (2) There was a significant difference among different emotion priming conditions in R (F(2,87) = 30.129, P＜0.05) .and there was no significant difference among different emotion priming conditions in A. (3) In R,there was a main effect in emotional words(F(2,81) = 10.516, P＜0.05) ,the R scores of neutral words were significantly higher than those of negative words(P＜0.01) ,there was a main effect in emotion priming conditions(F (2,81) =10.516, P＜0.05) ,the R scores of positive emotion priming group and neutral emotion priming group were significantly higher than those of negative emotion priming group (P＜0.05). There was an interaction of emotional words and emotion priming conditions(F(4,81) = 10.640, P ＜ 0.01). Under positive emotion priming condition, the R scores of positive and neutral words were significantly higher than those of negative words (P＜0.05);under negative emotion priming condition, the R scores of negative words were significantly higher than those of positive words (P ＜0.05); under neutral emotion priming condition, the R scores of positive and neutral words were significantly higher than those of negative words (P＜0.05). (4) In A, the main effect of emotion priming conditions and emotional words and interaction effect between them were not significant(P＞0.05). Conclusions Explicit memory showed mood congruency memory effect,but implicit memory,neither the positive emotion priming group nor the negative emotion priming group exhibited mood congruency memory effect.

Objective: To investigate the chemical constituents from the ethanol extract of Spiraea pubescens. Methods: The compounds were isolated and purified by chromatography on silica gel, ODS, and preparative HPLC. Their structures were elucidated on the basis of chemical and spectroscopic methods, including MS, 1D, and 2D NMR spectral techniques. Results : Fourteen compounds were isolated and identified as β-sitosterol (1), tricosyl alcohol (2), stigmast-4-en-3-one (3), pentacosyl alcohol (4), stigmastanol (5), (+)-cyclo-olivil (6), (+)-africannal (7), (+)-lyoniresinol (8), 5-methoxy-(+)-isolariciresinol (9), (+)-isolariciresinol (10), (7R,8R)-4,7,9,9'-tetrahydroxy-3,3'-dimethoxy-8-O-4'-neolignan (11), (6S,9R)-6-hydroxy-3-one-α-ionol-9-O-β-D-glucopyranoside (12), (+)-lyoniresinol 9-O-β-D-xylopyranoside (13), and (-)-lyoniresinol 9-O-β-D-xylopyranoside (14). Conclusion: Compounds 2-5 and 7-14 are isolated from the plants of Spiraea L. for the first time, and compounds 1 and 6 are obtained from this plant for the first time.

ObjectiveTo explore the effect of various forms of psychological intervention on psychological stress,mental state and treatment process of out- patients. Methods 198 outpatients from February 2010 to July 2011 were randomly divided into the observation group and the control group with 99 cases in each group.The control group received conventional treatment and care.The observation group was given multiple forms of psychological intervention on the basis of conventional treatment and care.Both groups were taken with Zung Anxiety Rating Scale (SAS)and the Hamilton Anxiety Scale(HAMA)to measure the psychological stress and the degree of anxiety before and after treatment.The total efficiencies of various disorders for the two groups before and after the diagnosis and treatment were compared. ResultsThere were no significant differences in SAS score and HAMA scores before psychological intervention.After the intervention,the SAS score and HAMA score in the control group were significantly higher than the observation group.The total effective rate after a course of treatment in the observation group was 90.6％.The total efficiency in the control group was 78.8％,which was significantly lower than that of the observation group. ConclusionsThe multiple forms of psychological intervention can reduce the mental stress,ease the patients' emotional state and improve the patients' therapy effect at the same period for out-patients.

Cardio-protective action of synthetic peptide 6A, fibrinogen degradation segment, was observed on the myocardial injury model produced by subcutaneous injection of iso-proterenol (30 mg?kg-1-d-1) into rat. Treatment with peptide 6A (50 ?mol?kg-1? d-1,iv) significantly ameliorated isoproterenol-induced myocardial lesion, inhibited release of myocardial creatine phosphokinase, ?-hydroxybutyratedehydrogenase, lactate dehydrogenase and gluta-mate-oxaloacetate transaminase, lowered plasma fibrinogen content,and markedly prevented myocardial calcium accumulation. The results suggest that peptide 6A could have potential significance for clinical therapy of ischemia heart diseases.

Rabbit blood samples were exposed to constant magnetic field within a range of intensity from 0.05 T to 0.35 T for 10 to 50 minutes, the propersities of hemorheology were measured by ektacytometry. The following results were obtained: The blood viscosity was lowed;The RBCs deformability was not changed obviously except for 10 minutes of exposure when the RBC＇s deformability was strongly decreased; The orientation index was increased; The results indicate that the magnetic field increases the RBC surface charge and results in the decrease of blood viscosity.

Aim To investigate the interactions be-tween the neuroinflammation caused by lipopolysaccha-ride(LPS) and brain CYP2E1.Methods The human cholinergic neuroblastoma cell line IMR-32 was treated with LPS ( 0.1 mg · L-1 , 1.0 mg · L-1 ) , and the LDH and SOD activities were determined after 24 h in-cubation .In order to determine the roles of MAPK sig-naling pathway in the regulation of CYP 2E1 by LPS, the IMR-32 cells were treated with p38 pathway inhibi-tor SB203580 or ERK pathway inhibitor U 0126 for 45 min before the incubation with LPS .The human do-paminergic neuroblastoma cell line SH-SY5Y with CYP2 E1 over-expression was established . The LDH and SOD activities were determined in SH-SY5 Y cells over-expressed CYP2 E1 and control cells treated with LPS(0.1 mg· L-1 , 1.0 mg· L-1 ) for 24 h.Results
The levels of LDH in IMR-32 cells treated with high-dose LPS were increased by 1.38-fold ( P <0.01 ) compared with the control group , and the levels of SOD reduced by 15.0%( P <0.01 ) .Compared with the control, CYP2E1 mRNA and protein levels in IMR-32 cells treated with high-dose LPS were increased by 1.25-fold(P<0.01) and 1.19-fold(P<0.05).The up-regulation of CYP2E1 by LPS could be attenuated by SB203580 and U0126 pretreatment.Compared with the control cells, the CYP2E1 over-expression in-creased LDH levels by 1.28-fold ( P<0.01 ) and de-creased SOD levels by 3.53-fold ( P<0.01 ) after the low-dose of LPS treatment .The CYP2E1 over-expres-sion increased LDH levels by 1.54-fold ( P <0.01 ) and decreased SOD levels by 2.17-fold( P<0.01) af-ter the high-dose of LPS treatment , compared with the control cells.Conclusions LPS can induce CYP2E1 mRNA and protein levels , and the p38 and ERK sig-naling pathway may be involved in the regulation .The elevated CYP2 E1 levels aggravate the damage to neuro-nal cells caused by LPS .Brain CYP2E1 may be an im-portant contributing factor to the pathological process of neuroinflammatory injury .