Objective To explore the effect of trans-axillary single-port and bilateral breast of ipsilateral axillary approach in endoscopic thyroidectomy on area of skin flap ,complications and the corresponding impact damage .Methods A total of 72 patients who were underwent either BBIAA or TASPET in our hospital from February 2013 to April 2015,were divided into BBIAA group and TASPET group ,each group had 36 cases.The area of skin flap ,complications and the corresponding impact damage between two surgical methods were compared .Results The average separation of the flap area in TASPET group was significantly higher than that in BBIAA group (P<0.01).The VAS score of BBIAA group was (0.24 ±0.39) point,which was lower than (1.85 ±1.37)point of TASPET group,the difference was significant(P<0. 01 ) .The patients of BBIAA group had no complications ,while there were 10 patients suffered complications in TASPET group ,and the differ-ence of postoperative complications was significant (P<0.01).Conclusion Bilateral breast and ipsilateral axillary approach have the advan-tage of less operative time ,smaller separation of the flap area ,lower complication incidence and less damage on parathyroid gland and nerve , which had an ideal safety and cosmetic result .

Objective To investigate the effects of cobalt chloride(COCl2)induced rat alveolar epithelial cell apoptosis and the significance of related apoptosis protein hypoxia inducible factor-lalpha(HIF-1 a)expression. Methods Alveolar epithelial cells were cultured in vitro and divided into four groups with 6 samples in each.Hypoxia 4 h group:added 500μmol/L COCl2 for 4 hours inducing alveolar epithelial cell hypoxia;hypoxia 24 h group:co-cultured with COCl2 for 24 hours;hypoxia 48 h group:co-cultured with CoCl2 for 48 hours;control group:no CoCl2 added.The apoptosis rates were detected by fluorescent microscope and flow cytometry.The apoptosis morphological changes of the cell were observed by transmission electron microscopy.The protein expression of HIF-la was detected bv Western Blot. Results (1)Fluorescent microscope results showed that apoptosis rate in hypoxia 4 h、24 h、48 h group[(5.83±0.76)%,(15.00±3.28)%and(51.50±3.00)%],were higher than control group[(1.50±0.50)%](P＜0.05).(2)Flow cytometry analysis reached the same results as with fluorescent microscope.(3)Typical morphological changes of apoptosis were observed in hypoxia 24 h group.(4)HIF-1α expression in hypoxia 4 h、24 h and 48 h groups[0.69±0.035,1.02±0.044 and 0.71±0.046],were higher than control group(0.21±0.026)(P＜0.01).(5)Positive correlations were found between relative amount of HIF-1αprotein and apoptosis rate by fluorescent microscope and flow cytometry analysis(r=0.484,P=0.016;r=0.713,P=0.009).Conclusions Hypoxia could induce apoptosis of alveolar epithelial cells,which may participate in the pathogenesis of hypoxia-induced lung injury.

To detect the effects of adrenaline and dexamethasone on proliferation of the lens epithelial cells and discuss the mechanism, the central area 5～7mm in diameter of the anterior capsule was obtained in cataract surgery (phacoemulsification, Phaco), and the specimens were divided into three parts, and were cultured in vitro and treated with 10 -6 mol/L adrenaline and dexamethasone for 48 hours. And then the positive area ratio of proliferating cell nuclear antigen (PCNA) in immunohistochemistry was estimated by medical color image analysis. The data were analyzed with the statistical software. The results indicated that the 10 -6 mol/L adrenaline and dexamethasone had obvious inhibition on LEC proliferation. The positive area ratio of PCNA was (2 614?0 922)%( t =5 594, P =0 0000) and (3 338?0 838)%( t =3 361, P =0 0013). It is suggested that adrenaline and dexamethasone could inhibit the proliferation of LEC and could be used to treat PCO after cataract surgery. The experiments provide the scientific basis for selection of drugs to prevent PCO after cataract.

Objective To observe the morphology, cell viability and secretion function of catecholamine of the alginate-polylysine-alginate (APA) microencapsulated bovine chromaffin cells (BCCs) before and after cryopreserving with liquid nitrogen. Methods The APA microencapsulated BCCs were cryopreserved with dimethyl sulfoxide as cryopreservative agent by slow cooling and rapid rewarming for revivification. The change of cell function was observed by detecting the cell viability and secretion of catecholamine. Results As compared with the precryopreseving cells, the morphology and cell viability of the resuscitated APA microencapsulated BCCs showed no significant change. The catecholamine secretion volume of BCCs remained 80% of that by the precryopreserving cells. Conclusion It demonstrates that the resuscitated cryopreserved APA microencapsulated BCCs still remained good morphology, cell viability and secretion function of catecholamine.

Objective To compare the clinical efficacy of esomeprazole or omeprazole triple therapies on gastric ulcer patients complicated with type 2 diabetes mellitus.Methods Seventy-five patients with Hprelated gastric ulcer and type 2 diabetes mellitus were enrolled and were randomly divided into group A(n=39)and group B (n =36).Patients in group A were treated with esomeprazole,amoxicillin and clavulanate potassium dispersible tablets,Clindamycin.Patients in group B were treated with omeprasole,amoxicillin and clavulanate potassium dispersible tablets,Clindamycin.Patients in both groups were treated with hypoglycemic therapy.The efficacy and the Hp eradicate rate of the two groups were observed and compared.Results The cure effect in group A was 97.44％(38/39),much better than that of group B(80.56％(29/36);x2=5.598,P=0.025).The Hp eradicate rate in group A was 92.31％,also much better than that of group B(75.00％;x2 =4.172,P =0.041).Conclusion The clinical egicacy and the Hp eradicate rate of esomeprazole triple therapies on gastric ulcer patients complicated with type 2 diabetes mellitus is better than that of omeprazole triple therapies.Esomeprazole triple therapies and it is worthy of being clinically promoted.

Objective To introduce and translate the Coping-adaptation Processing Scale:Short Form-15 items (CAPS:SF) and evaluate the reliability and validity among people after losing the only-child. Methods CAPS:SF was translated into Chinese (CV-CAPS:SF) according to Brislin. A sample of 77 people after losing the only-child were recruited to complete the scale to evaluate the reliability and validity of scale. Results The scale-content validity index of the CV-CAPS:SF was 0.94;the Cronbachαwas 0.85 and split-half coefficient was 0.84. The score of coping was (38.60 ± 5.30) points. The take strength from spirituality or the successes of courageous people was the highest (3.44±0.50) points;the use humor in handling the situation was the lowest (2.05±0.58) points. Different age, education degree, marital status, occupation, average monthly income of latest year, the length after losing the only-child in the face of the difference was statistically significant (t/F=7.636-85.130, all P<0.05). Conclusions The CV-CAPS:SF is reliable and valid. It could be used to help the nursing staff understand coping among people after losing the only-child .

Objective To study the value of Oxytocin challenge test (OCT) in high-risk pregnancy with abnormal umbilical artery resistance and the clinical significance of performing the umbilical velocimetry during OCT. Methods One hundred and seventy patients with abnormal umbilical artery resistance but maintained forward diastolic flow and 178 patients with normal umbilical blood flow received OCT, umbilical velocimetry was conducted during OCT. If the umbilical artery pulsatility index (PI) increased more than 0.5 during contraction and OCT was positive, cesarean section was performed in the same day, otherwise, vaginal delivery was tried. Results The rate of positive OCT was 45.3% and 10.1% respectively and the rate of positive OCT with umbilical artery PI increasing more than 0.5 was 37.4% and 6.7% respectively in abnormal umbilical artery flow resistance group and normal umbilical artery flow group ( P

Objective:To approach the effectiveness and safety of traditional Chinese medicine combined with risperidone in the treatment of schizophrenia,and to provide basis for their application in clinic. Methods:The Cochrane,Pubmed,EMBase,CNKI,Wanfang and VIP databases were used to retrieve the randomized controlled trials (RCTs ) and CCTs about traditional Chinese medicine combined with risperidone in the treatment of schizophrenia by computer;and manual retrieval was performed at the same time.The literatures met the standard were collected.The qualities of all the included studies were assessed by Cochrane Handbook and Meta-analysis was performed by use of the RevMan 5.2 software. Results:Fifteen trials and 1 314 patients were included in this review. The qualities of 11 studies were grade C.The Meta-analysis results showed that the clinical effective rate in traditional Chinese medicine combined with risperidone treatment group was higher than that in control group,the difference between two groups was statistically significant (OR= 2.11,95% CI: 1.59 - 2.81,P < 0.01 ).The incidence of adverse drug reactions (insomnia,extrapyramidal symptoms,akathisia)in traditional Chinese medicine combined with risperidone treatment group was lower than that in alone risperidone group, and there were significant differences between two groups (OR=0.25,95%CI:0.17-0.36,P <0.01).Conclusion:Traditional Chinese medicine combined with risperidone is superior to simple application of risperidone in the treatment of schizophrenia,and they can reduce the occurrence of adverse effects of medicine.

Bronchopulmonary dysplasia( BPD) is a chronic lung disease that commonly occurs in pre-term. Since the 1980s,with the advances in perinatal care,survival rate of preterm has been raised,while BPD continues to be a significant cause of morbidity and mortality for premature infants. However,the treatment effect of BPD is unsatisfactory. It is urgent to search for innovative ways to reduce the respiratory injury that caused by BPD as well as to improve the quality of life. In recent years,mesenchymal stem cells′( MSC) research has pro-vided a new way for the treatment of BPD. This review primarily summarizes the current status of the MSC ther-apies and its possible mechanism on BPD.

AIM: To assess the effect of two different suture methods on the degree of pain and corneal epithelium healing condition after pterygium excision combined with autologous conjunctival flap graft transplantation. ·METHODS: Retrospective case-series study. According to the suture method, a total of 92 patients (92 eyes) with pterygium who received treatment in the First Affiliated Hospital of Huzhou University from June 2015 to June 2016 were divided into two group. There were 48 patients ( 48 eyes) in Group A were received intermittent suture, and 44 patients ( 44 eyes ) in Group B were received continuous interlocking suture. The degree of pain after surgery were evaluated between the two groups at 2h, 1d and 1wk after surgery by visual analogue score ( VAS). The healing status of corneal epithelium were observed between the two groups at 1d and 1wk after surgery by fluorescent staining. ·RESULTS: There was no significant difference in the average pain value 2h after surgery between Group A and Group B (P>0. 05). The average pain values 1d and 1wk after surgery in Group B was lower than that in Group A respectively (P<0. 01). Mean scores of corneal epithelium healing condition at 1wk were significantly better in Group B than in Group A (0. 54 ± 0. 32 vs 0. 86 ± 0. 34, P<0. 05), while not significantly different at 1d after the surgery (4. 04±1. 46 vs 4. 30±1. 42, P>0. 05). · CONCLUSION: Compared to intermittent suture, continuous interlocking suture can release pain response after pterygium excision combined with autologous conjunctival flap graft transplantation and promote the healing status of corneal epithelium.