Erythroblastosis, Fetal ; blood ; diagnosis ; Female ; Humans ; Infant, Newborn ; MNSs Blood-Group System

Objective: To discuss the microanatomy of anterior inferior cerebellar artery (AICA) and to provide related microanatomical basis for surgery in the area of cerebellopontile angle. Methods: Twenty AICAs and their branches at both sides in 10 adult brain specimens were observed anatomically and were measured under an operative microscope. Results: The frequency of bilateral AICAs was 95% and double branches on the same side in one case (5%), The frequencies of their branching labyrinthine artery, recurrent perforating artery, subarcuate artery, and cerebellar subarcuate artery were 95%, 80%, 70%, and 5%, respectively. The frequency of AICA formed the AICA loop near the facial nerve root was 90%. The diameter of AICAs was 1.29 ± 0.43 mm (0.56 - 1.87 mm). The AICA loop was closely associated with the internal auditory foramen, and 80% penetrates between facial nerve and auditory nerve. Conclusion: Being familiar with the microanatomy of AICA helps avoid the damage of related arteries in the area of cerebellopontine angle during operation.

AIM:To compare the influence of anterior chamber maintainer and viscoelastic agent on corneal astigmatism and endothelial cells after phacoemulsification.METHODS:Totally 70 patients(70 eyes) of cataract from April 2013 to April 2015 were randomly divided into the study group and the control group, with 35 cases in each group.The study group were treated with anterior chamber maintainer during phacoemulsification with support system approach, and the control group were treated with phacoemulsification under viscoelastic agent.RESULTS:The age (t=0.215, P=0.831), the density of corneal endothelial cells (t=-0.352, P=0.726) and corneal luminosity (t=-0.162, P=0.872) of two groups had no significant difference before surgery;there were no significant difference in preoperative visual acuity (t=0.463, P=0.599) and visual acuity (t=1.616, P=0.124) at 1mo after operation.And patients in the study group (t=-21.129, P<0.01) and the control group (t=-12.780, P<0.01) before surgery and 1mo postoperative when compared with the naked eye eyesight showed significant difference.The visual acuity after operations improved significantly.There were significant differences of corneal endothelial cells density (t=8.489, P< 0.01) and corneal astigmatism (t=-2.032, P=0.046) in the study group before surgery and 1mo after surgery;corneal endothelial cell density (t=8.999, P<0.01) and corneal astigmatism (t=-2.167, P=0.034) in the control group before surgery and 1mo after surgery also had significant differences.There was no significant difference in the rate of corneal endothelial cell loss between the two groups (t=0.410, P=0.683).CONCLUSION:Compared with viscoelastic agent, anterior chamber maintainer during phacoemulsification in patients with cataract won't increase the damage of postoperative surgically induced astigmatism and corneal endothelial cells, which mean the method of anterior chamber maintainer during phacoemulsification in the treatment of cataract is safe and effective.

Animals ; Humans ; Spectrometry, Mass, Electrospray Ionization ; Spectrophotometry, Atomic ; Thallium ; analysis ; metabolism

Objective To develop a new method to repair the defect after enlarged excision of dermatofibrosarcoma protuberans.Methods This study included 8 patients with dermatofibrosarcoma protuberans measuring 2.5 to 5.5 cm in diameter.All the patients underwent enlarged excision of the affected skin and subcutaneous tissue.The defect measured 12.5 to 17.5 cm in diameter.Sieve skin flaps secured with a vacuum-assisted closure device were used to repair the huge surface defects.Results All the patients experienced the survival of sieve skin flaps at stage Ⅰ after operation,with no infection,effusion or necrosis.No relapse was observed during the 3 to 40 months of follow up.A satisfactory recovery was achieved in skin appearance and function with the formation of a flat scar,and no obvious proliferation occurred.Conclusion The vacuum-assisted closure device offers a safe and simple method for securing skin grafts to the defect after enlarged excision of dermatofibrosarcoma protuberans.

Objective To investigate the role of arcuate fasciculus in conduction aphasia after ischemic stroke using magnetic resonance diffusion tensor imaging (DTI) technique. Methods Five patients with conduction aphasia after acute ischemic stroke (case group) and 5 patients without aphasia (control group) were selected. The DTI technique was used to detect the fractional anisotropy (FA) of Broca's area, Wernicke's area, and arcuate fasciculus. Results The FA values of Broca's area (0. 341 ± 0. 018 vs. 0. 295 ± 0. 060; t = 4. 533, P = 0. 045), Wernicke's area (0. 303 ± 0. 009 vs. 0. 243 ± 0. 011; t = 6. 556, P = 0. 022), and arcuate fiber bundle (0. 646 ± 0. 020 vs. 0. 541 ± 0. 009; t = 7. 343, P = 0. 018) in the left hemisphere were significantly higher compared with their mirror areas in the control group. The FA values of Broca's area (0. 315 ± 0. 015 vs. 0. 280 ± 0. 006; t = 6. 246, P = 0. 023), Wernicke's area (0. 299 ± 0. 012 vs. 0. 239 ± 0. 022; t = 4. 576, P = 0. 025), and arcuate fiber bundle (0. 226 ± 0. 016 vs. 0. 294 ± 0. 007; t = - 12. 590, P = 0. 006) in the left hemisphere were significantly higher compared with their mirror areas in the case group. There was no significant difference Broca's area (t = 1. 901, P = 0. 13) and Wernicke's area (t =2. 092, P = 0. 105) in the left hemisphere between the case group and the control group, but the FA values of arcuate fasciculus in the left hemisphere in the case group was significantly lower compared with the control group (t = 28. 393, P < 0. 001 ). Conclusions For patients with conduction aphasia after acute ischemic stroke, the arcuate fasciculus has the left-dominant lateralization. Their conduction aphasia may be associated with the reduction and break of the arcuate fasciculus.

Female ; Humans ; Infant ; Male ; Ultrasonography ; Urachus ; abnormalities ; diagnostic imaging

Objective To research the effect of Bifid Lriple Viable on validity for adjuvant treating ulcerative colitis,colon mucosa IgA and sera immunity indexes in patients with ulcerative colitis.Methods The patients with ulcerative colitis in our hospital from November 2004 to June 2006 were randomized into experiment group and control group.The patients in experiment group were treated with Bifid Lriple Viable and routine treatment,however the patients in control group received only routine treatment.The clinical symptom score,colon mucosa inflammation score,colon mucosa IgA expression,sera T lymphocyte subgroup,sera immunoglobulins and complement C3 and C4 were compared between two groups before treatment and two months after treatment.Results The clinical symptom score and colon mucosa inflammation score and colon mucosa IgA expression,sera T lymphocyte subgroup,sera immunoglobulins and complement C3 and C4 had no significant difference between two groups before treatment.Two months after treatment,the clinical symptom score and colon mucosa inflammation score descended significantly(P0.01),but the ascending value of ratio of CD4+ to CD8+ T lymphocyte was more in experiment group than control group(P

Objective To study the psychological changes and the effect of psychological intervention and to discuss the influence of psychological rehabilitation on carcinoma patients after comprehensive interventional therapy.Methods 100 carcinoma patients were divided into two groups randomly:the psychological intervention group(50 casea) received both psychological intervention therapy and comprehensive intervention therapy;the control group (50 cases) received only comprehensive interventional therapy;Curative effects were assessed with the Symptom Checklist90(SCL-90) ,the Self Rating Depression Scale(SDS) and the Self Rating Anxiety Scale(SAS) for each patient. Results For the psychological intervention group, SCL-90 scores after therapy was significantly lower than that before therapy ( P < 0. 05 ), and were also significantly lower than that ( without anxiety agent) of the control group. The scores of SDS and SAS in post-therapy patients were significantly lower than that of pre-therapy patients in the psychological intervention group. The scores of SDS had no statistical difference between post-therapy patients and pre-therapy patients in the control group. The scores of SAS in post-therapy patients was significantly lower than that of pre-therapy patients in the control group. Both scores of SDS and SAS in the control group were significantly higher than that of the psychological intervention group after therapy. Conclusion Psychological intervention for carcinoma patients after comprehensive interventional therapy played an promotional role in psychological rehabilitation in the community.

Background Brn-3a is a newly discovered specificity marker for retina ganglion cells(RGCs).It is well-known that RGCs damage is a important pathological basis of hypertension-visual disorder.But the study concerning expression of Brn-3a in RGCs in glaucoma eye is still rately.Objective The purpose of this work was to investigate the changes of Brn-3a expression in model eye with chronic high intraocular pressure(IOP) and its relation with morphology of retina and the expression of Brn-3a in chronic ocular hypertension rats.Methods Thirty-five clean adult SD rats were randomly divided into normal control group(5 rats) and model group(30 rats).Experimental chronic ocular hypertension models were induced unilaterally in the left eyes of 30 health adult SD rats by cauterizing super-scleral veins,and the conjunctival incision was made in the right eyes as sham operative group.The operated rats were subdivided into 6 groups according to the examination time points and 6 rats for each group.IOP was measured with Tono-Pen tonometer before and after 30 minutes,1,3,7,14,28 day after surgery respectively.The rat models were sacrificed in 1,3,5,7,14,28 days after operation by excessive anesthesia method,and retinal section was prepared for the histopathological examination and the RGCs were counted using Nissl staining method.Expression of Brn-3a in RGCs was detected by immunohistochemistry.This experimental complied with the Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission.Results The IOP was significantly raised from 30 minutes to 28 days after operation in model eyes with the top IOP( 34.46±4.65 )mmHg in the 30 minutes after operation,showing statistically significant differences in various time groups ( F =95.631,P =0.001 ) and different eyes ( F =287.473,P =0.001 ).Compared with sham operative group,the IOP were elevated from 1 day through 14 days after operation ( q =18.418,15.261,10.987,6.931,4.975,2.962,P ＜ 0.05 ).The numbers of RGCs were ( 29.08 ± 1.98 ) in the normal control group and decreased gradually by 3.17％,7.84％,14.60％,22.40％ in 1,7,14,28 day after surgery in the model eyes with the considerable differences in comparison with normal eyes ( t =5.943,8.034,15.023,17.004,19.371,P ＜ 0.05 ).Immunochemistry revealed that Brn-3a was specially expressed in the RGCs layer and the positive RGCs for Brn-3a were evidently decreased as the prolong of high IOP duration ( F =127.583,P =0.000 ).Conclusions Chronic glaucoma model can be successfully established using Shareef-Sharma method.The loss of RGCs is associated with the high lOP duration.The expression level of Brn-3a is a reliable index of high-IOP-induced damage of optical nurve.