Objective: To analyze the uncertainty in the determination of formaldehyde in food stimulant.Methods: HPLC with pre-column derivation was used to determine formaldehyde in food stimulant.A calculation formula was established to identify the source of uncertainty and the combined uncertainty was evaluated.Results: The synthetic uncertainty in the determination of formaldehyde was 1.7% and the expanded uncertainty was 3.4%.The formaldehyde content in food stimulant was (1.320 ± 0.044) mg·L-1 (k =2).Conclusion: The study provides reference for the uncertainty analysis on formaldehyde content in food stimulant by HPLC with pre-column derivation.

OBJECTIVE:To provide basic industrial research and development recommendations for the supply-side structural reform in drug distribution in China. METHODS:Structure-conduct-performance(SCP)analysis paradigm from Harvard was used for in-depth study of market structure,business conduct and business performance in China since 2010. RESULTS:Since 2010, drug distribution industry in China had shown the scale of decline in the growth rate,low market concentration,increased barriers to entry,the leading of state-owned capital,etc. Though corporate mergers and acquisitions and public financing expanded the scale,management information,third-party logistics,Internet+and pharmacy alliance business model flourished,the industry op-erating performance was poor,state-owned enterprises showed poor performance. CONCLUSIONS:The transformation and upgrad-ing of the industry business model should be promoted by targetedly accelerating the supply-side structural reforms in the drug distri-bution industry,encouraging corporate finance mergers,breaking local protectionism,developing cooperative pharmacies and phar-maceutical third-party logistics alliances and leveraging the Internet.

By direct communication between clinicians and laboratorians, obstacles between clinical work and laboratory diagnosis were unequivocally found at an academic conference. Many clinical physicians feel disappointed and frustrated at the laboratory diagnosis by semen analysis and frankly express their expectations, as to recognize the role and significance of laboratory diagnosis, understand the value of routine semen analysis, work out the puzzles in the analysis of sperm morphology, fulfil the requirements for the identification of cells in semen, and obtain responses from and initiate discussions among laboratorians about the relevant questions. Both laboratorians and clinicians are appealing for strengthened management and earlier establishment of national standards and operating specifications for laboratory diagnosis by semen analysis.
Clinical Laboratory Techniques ; Communication ; Humans ; Interprofessional Relations ; Male ; Physicians ; Semen ; Semen Analysis

ObjectiveTo explore the risk factors of pulmonary complications in children with mycoplasma pneumoniae pneumonia.MethodsClinincal data of 341 children with mycoplasma pneumonia were retrospectively analyzed.Children under 2 months were divided into study group and control group according to X-ray or lung CT,the study group of children left lung sequelae in children and the control group without sequelae.Using statistical analysis methods to study the children age,sex,hot process,symptoms,signs,lung lesions,lesion type,white blood cells,erythrocyte sedimentation rate,C-reactive protein,antibiotics,gamma globulin,glucocorticoid application.ResultsAt electasis 30 cases,3 cases of bronchiectasis,bronchiolitis obliterans in 1 case.Logistic regression analysis showed that children fever ＞ 10d,pleural effusion,lesion in the right upper lung,X-ray showed a large shadow,pulmonary complications in children with pulmonary high risk of complications,including chest plot sequelae in children with lung fluid was a strong risk factor( P ＜ 0.01,OR =7.684).ConclusionChildren of fever ＞ 10d,pleural effusion,lesion in the right upper lung,X-ray showed a large shadow,pulmonary complications in children with pulmonary high risk of complications,clinical treatment should be noted.

Objective To investigate the influence of genistein(Gen) on the growth and apoptosis regulation of hepatoma carcinoma cells SMMC-7721.Methods SMMC-7721 cells were divided into three Gen groups treated with different concentrations(5.0,10.0,20.0 mg?L-1) and control group(without Gen).After SMMC-7721 cells were treated for 24 and 48 h,the changes of ultrastructure of cells were observed under electron microscope, MTT was used to detect the inhibitory rate of proliferation of SMMC-7721 cells,flow cytometry was performed to analyze cell phase;immunohistochemistry was used to detect the expression of Caspases-3 protein.Results Compared with control group,chromatin in cellular nucleus became conglomeration or lumping,with chondriosome swelling in Gen groups.With the increasing of Gen concentration,the cellular nucleus became condensed,heterochromatin agglutinating,ground cytoplasm obviously cavitating;with the increase of Gen concentration and prolongation of time,from 24 to 48 h,MTT showed the inhibitory rate of proliferation of SMMC-7721 cells increased in a time-and dose-dependent manner.Flow cytometry analysis showed that with the increase of Gen concentration,the number of cells at G2/M phase increased,the number of cells at S phase decreased and the ratio of G0/G1 also decreased.Immunohistochemistry results showed the expression of protein Caspases-3 increased with the Gen concentration in a dose-dependent manner(P

OBJECTIVE To investigate the TORCH infection of women with childbearing age in Chongqing.(METHODS) Special IgM antibodies were detected by the TORCH-IgM screen test kits in 2067 women with(childbearing) age,among them 1829 were women with normal childbearing and 238 were women with abnormal one.RESULTS The infection rate of Toxoplasma gondii(TOX),Rubella virus(RV),Cytomegalovirus(CMV) in women with abnormal childbearing was significantly higher than those in women with normal one(P(0.05)).CONCLUSIONS The(abnormal) pregnancy is (related) with TORCH infection.So TORCH screen before pregnancy is very important to aristogenesis.

[objective] To investigate the effects of curcumin on the behavior of chronic constrictive injury(CCI)rats and the c-fos expression in spinal cord dorsal horn.[Methods]108 male SD rats were randomly divided into 4 groups:Control group(treated with CCI);Sham operation group;Solvent contrast group;Curcumin treated group.Thermal withdrawal latency(TWL)and mechanical withdrawal threshold(MWT)of rats were determined by Hargreave's thermal radiation apparatus and von Frey's method of microfilament,respectively.The time course of expression of c-fos were assessed by immunohistochemical analysis.[Results]Curcumin could significantly attenuate the activation of c-fos induced by CCI and simultaneously ameliorate thermal and mechanical hyperalgesia induced by CCI.[Conclusion]This study shows that curcumin could attenuate the activation of c-fos to ameliorate the CCI-induced neuropathic pain.

Objective To observe the therapeutic effect of segmental scleral buckling and vitrectomy with/without lensectomy on the retinopathy of prematurity (ROP) stage 4a,4b and 5.Methods One hundred and thirty-four ROP infants (181 eyes) diagnosed as stage 4a,4b and 5,and performed with segmental scleral buckling or vitreous with/without lensectomy were retrospectively analyzed.The operated 4a-,4b-and 5-stage eyes were 40,51 and 90 eyes.The operational method depended on the location and severity of fibrovascular membrane.Of 181 eyes,segmental scleral buckling was referred for 37 eyes which include 23 eyes with 4a stage and 14 eyes with 4b stage;vitrectomy was referred for 50 eyes which include 14 eyes with 4a stage,29 eyes with 4b stage and 7 eyes with 5 stage;vitrectomy with lensectomy was referred for 94 eyes which include 3 eyes with 4a stage,8 eyes with 4b stage and 83 eyes with 5 stage.The effect was classified as success,improved and failure.Failure includes lost eye.Follow-up for 4a,4b and 5 stage patients are 34,31 and 29 months respectively.Results Segmental scleral buckling was referred for 37 eyes,success in 23 eyes (62.16％),improved in 11 eyes (29.73 ％),failure in 3 eyes (8.11 ％).Vitrectomy was referred for 50 eyes,and success in 20 eyes (40.00％),improved in 22 eyes (44.00％),and failure in 8 eyes (16.00％).In the total of 94 eyes underwent vitrectomy with lensectomy,20 eyes was success (21.28％),improved in 17 eyes (18.08％),failure in 57 eyes (60.64％).In 40 stage 4a eyes,33 successes (82.50％),6 improved (15.00％) and 1 failure (2.50％).In 51 stage4b eyes,11 successes (21.57％),30 improved (58.82％) and 10 failures (19.61％).For 90 stage 5 eyes,14 successes (17.50％),19 improved (23.75％) and 57 failures (71.25％).The therapeutic effect of segmental scleral buckling for stage 4a was better than that for stage 5 (x2 =6.707,P=0.035).The difference of therapeutic effect of vitrectomy for different stage was significant (x2=21.010,P =0.000);stage 4a was the best;stage 4b was the second,stage 5 was the worst.The therapeutic effect of vitrectomy with lensectomy for stage 5 was worse than that for stage 4a and 4b (x2=16.066,P=0.003).Conclusion The surgery patterns of ROP was determined based on the disease severity,the surgery effects of stage 4a and 4b were better than stage 5,which had nothing to do with the surgical procedures.

Objective To investigate the curative effects of oxalic acid Ai Sciplan in assisting psychological therapy for coronary heart disease accompanied with depression. Methods A total of 136 patients of coronary heart disease with de?pression were randomly divided into control group and treatment group. The routine drug treatment was given to control group. Treatment group was given oxalic acid Ai Sciplan which is assisted by the psychological therapy based on the routine drug treatment schedule for six months. HAMD score before treatment, one-week, one-month, 3-month and 6-month after treatment were compared between two groups. Homocysteine (Hcy) and high sensitive C reactive protein (hs-CRP) levels were detected by circulating enzymatic method before treatment and 6-month after treatment. The WHO quality of life scale (WHOQOL-BREF) was used to assess the quality of life in two groups of patients. The improvements of angina and depressive symptoms were evaluated after 6-month treatment. The cardiovascular events were followed up in two groups. Re?sults HAMD scores significantly decreased after treatment in both groups (P < 0.05). HAMD scores at all different time points were significantly lower after treatment than those before treatment in treatment group. In control group, HAMD scores at different time points were also significantly lower after treatment than those before treatment, except time point of one week after treatment. The levels of Hcy and hs-CRP were decreased after treatment in two groups, and the levels were lower in treatment group than those of control group. Values of WHOQOL-BREF score, mental status, social relations and the sur?rounding environment scores were higher after treatment in both groups. Also they are higher in treatment group than that of control group. The angina and depressive symptoms were improved in treatment group. The incidences of angina, myocardial infarction, arrhythmia, heart failure, sudden cardiac death and other cardiovascular events were significantly lower in treat?ment group than those of control group (χ2=9.396, P<0.05). Conclusion Escitalopram oxalate combined with psychological therapy shows a significant beneficial effect and a better prognosis in the treatment of coronary heart disease and depression.

Objective To evaluate the usefulness of diffusion-weighted MR combined with routine T2 WI in finding the possible residual foci in uterine cervical cancers after radical chemo-radiation therapy.Methods This was a retrospective study including 25 consecutive cervical cancer patients who received hysterectomy after radical chemo-radiation therapy.All of them underwent MR examinations post-chemoradiation and just before operation.Images of T2 WI alone and those of T2 WI combining DWI were evaluated respectively by 2 senior radiologists,in order to decide whether there were residual tumors.ADC values were also measured.Taking the post-operation pathological results as the gold standard,the accuracies,sensitivities and specificities of T2 WI alone,T2 WI combining DWI,and ADC values were all calculated.Results In those 25 patients,9 were found with foci of residual cancer in operative pathology,while no cancer cells were found in the other 1 6 patients.The accuracy,sensitivity and specificity in finding the positive residual cancer using T2 WI alone were 56.0%,77.8% and 43.8%,comparing with 72.0%,66.7% and 75.0% in T2 WI combining DWI.The accuracy and specificity increased with statistical significance after combining DWI (P =0.01 6 for accuracy,P =0.031 for specificity),while the sensitivity decreased but did not reach statistically significant level (P =0.099).No difference in ADC values was found.Conclusion DWI can be used as a supplementary sequence in finding the existence of residual tumors of cervical cancer after radical chemo-radiation therapy.Routine T2 WI combing DWI increased the specificity and accuracy,but still facing the risk of decreasing sensitivity.