Objective To investigate the effect of hypoxia on vascular endothelial growth factor (VEGF) expression and secretion in human renal fibroblasts. Methods Human renal fibroblasts were primary cultured from donor kidney, and cells in passage 3 were used in this experiment. Hypoxia was induced by incubation in a gas-tight box gassed with 10% CO2/10% H2/80% N2 for 24 hours. VEGF mRNA levels were measured by semi-quantitative RT-PCR method. VEGF proteins expressed on cell surface were measured by flow cytometry. VEGF proteins secreted into supernatants were measured by ELISA method. Results Hypoxia caused a 2. 5-fold increase of VEGF mRNA level in human renal fibroblasts. Hypoxia also induced significant increases of VEGF proteins on cell surfaces (mean fluorescence intensities: 1. 053?0. 055 vs 0.763?0.057, n=4,P

Objective To compare hip arthroplasty versus internal fixation for treatment of displaced femoral neck fractures in the elderly patients in terms of reoperation,surgery-related complications and postoperative 1-2-year mortality.Methods All the randomized controlled trials (RCTs) up to May 2013 on hip arthroplasty versus internal fixation for displaced femoral neck fractures in the elderly were electronically searched for in Pubmed/Medline,EMBASE,Cochrane CENTRAL,CNKI,WanFang and VIP databases.The 2 treatments were compared in terms of reoperation,surgery-related complications and postoperative 1-2-year mortality.The Meta analysis was performed with RevMan 5.2.5 software.Results Five eligible RCTs involving 1 288 cases were included for this review.The rate of reoperation (including implant removal and other operations),either within 2 years or over 2 years,in the internal fixation group was significantly higher than that in the arthroplasty group (P ＜ 0.05).The surgery-related complications in the internal fixation group was significantly higher than that in the hip arthroplasty group [OR =8.82,95％ CI (6.45,12.07),P ＜0.001].No significant difference was found in the postoperative 1-2-year mortality between the 2 groups (P ＞0.05).Conclusion In the treatment of displaced femoral neck fractures in the elderly,compared with internal fixation,arthroplasty should be recommended as the first choice,because it can significantly reduce surgery-related complications and reoperations though it cannot decrease the postoperative 1-2-year mortality.

AIM:To evaluate the efficiency of vitrectomy combined with inferior scleral buckling for a special kind of retinal detachment.
METHODS: Nineteen eyes of special retinal detachment had following features: 1 ) the course was more than 6mo;2) there were at least one hole located in the 5 : 00 - 7 : 00 of the marginal retina; 3 ) the detachment range of retina more than 270 degrees and retinal proliferation in retinal detachment. The 19 eyes who received vitrectomy combined with inferior scleral buckling, retinal anatomic reattachment and visual function recovery was observed.
RESULTS: Among these 19 eyes, all eyes retinal anatomic reattachment and best corrected visual acuity ( BCVA ) was improved in various degrees. The BCVA was 0. 01-0. 1 in 5 eyes, 0. 12-0. 3 in 9 eyes, and ≥0. 4 in 5 eyes.
CONCLUSION: It is an effective method to vitrectomy combined with inferior scleral buckling for the special kind of retinal detachment which has following features:1) the course was more than 6mo;2) there were at least one hole located in the 5:00-7:00 of the marginal retina;3 ) the detachment range of retina more than 270 degrees and retinal proliferation of cable in retinal detachment.

Objective To investigate the best dignostic cut-off point of non-typical ectopic pregnancy(EP) through the determination of vascular endothelial growth factor(VEGF)in 154 cases of suspected ectopic pregnancy patients.Methods The serum level of VEGF in 154 cases with suspected EP was determined by means of immunossay technique,and the results were made into ROC-Curve.Results The acreage behind the curve was 0.95,illustrating that the VEGF can be as a better marker for early diagnosis of EP.The serum VEGF level of 200pg/ml was the cut-off point for early diagnosis of EP throash ROC-Curve.Condusion The serum VEGF can be considered as an early diagnostic marker of EP,which cut-off point is benifical to reducing misdiagnosis of EP.

Objective To evaluate our early results of a combined minimally invasive surgery with surgical procedures for critical extremity ischemia. Methods From July 1999 to October 2000 intraluminal iliac minimally invasive surgery combined with simultaneous conventional surgery were performed on 20 lower extremities of 15 pa- tients suffering from critical multilevel atherosclerotic occlusive disease. There were 11 men and 4 women with an aver- age age of 73 years. The indications for surgery included disabling claudication in 6, rest pain in 9(included tissue gan- grenes in 3)patients. Mean preoperative ankle - brachial index(ABI)was 0. 35 ? 0. 18. Results 17 iliac mini mal- ly invasive approaches combined with simultaneous 11 femoro - popliteal bypass and 3 femoro - femoral popliteal by- pass as well as 5 profundaplasty were performed in 20 limbs of 15 patients. minimally invasive surgery were technically successful in all patients. One femoral - popopliteal bypass was failure. There were no additional instances of procedural or postoperative morbidity or mortality, and no wound - related complications. Postoperative ABI of femoral - popliteal bypass was 0.74 ? 0. 16(p

Objective:To investigate whether the AP-PCR fingerprint of mutans streptococci(MS) can be displayed by PAGE-AgNO 3 staining. Methods: Amplification products of 200 MS clinical isolates by AP-PCR was separated and stained by ?=3.5%PAGE-AgNO 3 and agarose-EB respectively. Results were compared and the agreement value of Kappa between two methods was calculated. Results: ?=3.5%PAGE-AgNO 3 discerned both homogeneity and heterogeneity of MS genotypes, just as agarose-EB,Kappa value for agreement was 1.00 . Moreover, more bands was showed by PAGE-AgNO 3 staining than by agarose-EB, so PAGE-AgNO 3 gave a clearer pattern than agarose-EB. Conclusion: AP-PCR fingerprint of MS can be displayed by ?=3.5%PAGE-AgNO 3 staining.

OBJECTIVE:To introduce coordinate localization method as a new pharmaceutical care method to provide reference for rational use of drug in cancer patients. METHODS:The way that clinical pharmacists assisted physicians in prescribing for cancer patients with coordinate localization method was interpreted combining with examples. RESULTS:Coordinate localization method was conducive to rational use of drug in cancer patients and pharmacist’s self- quality construction. CONCLUSION:Coordinate localization method can effectively assist the doctor to prescribe rationally.

Objective To discuss the practicability of application of fault tree analysis in innovation to electrical system of SL-IE radiotherapy simulator.Methods The structure and principle of the electrical system were analyzed,the fault tree for the electrical system was built,and the qualitative analysis and quantitative analysis to the fault tree were made.The electrical system was innovated with effective redundant method,and the qualitative analysis and quantitative analysis were made after innovation.Theoretical analysis and the actual validation were made for the fault tree of electrical system after innovation.Finally,several notes were provided for the innovation to the similar medical equipments.Results The fault tree analysis method was applied to innovating the electrical system,and theoretical analysis indicated that the failure probability to total system reduced obviously.Actually,the actual effect after innovation also validated the correctness of the theoretical analysis.Conclusion Application of fault tree analysis in innovation to electrical system of SL-IE radiotherapy simulator is practical.But,the selection to the innovation event,the change to maintainence after innovation and the event to innovate carefully should be paid attention to.

Objective:To Observe the effect of recombinant tissue-type plasminogen activator (rt-PA) in treatment of acute myocardial infarct.Methods:Two groups of patients were observed.The subjects in the control group (35 patients) were treated with routine intervention,while those in thrombolysis group (15 patients) were treated with rt-PA in addition to the routine intervention.Results:There is a better outcome in the thrombolysis group as compared with that in the control group,in terms of length of angina relief,degree and length of EKG changes as well as the time for serum enzyme changes.The mortality rate in the thrombolysis group was significantly lower than that in the control group (P＜0.01).Conclusion:The rt-PA for thrombolysis in acute myocardial infarct patients can reduce the infarct size and improve the cardiac function rapidly as well as increase the survival rate.

Objective To study the value of optimal-angle transmission after 3D reconstruction for the display of aneurysm neck of intracranial aneurysm. Methods INNOVA3100 DSA machine and 34 cases with intracranial aneurysm got involved in. Results With conventional DSA, there were 8 effective results and 26 uneffective ones, while with the method of rotational DSA, the number of effective resuls came to be 31, and the number of uneffecive results became 3. Conclusion DSA acquisition after optimal-angle transmission is better than conventional DSA in the display of intracranial aneurysm neck.