Objective To investigate the correlation between the serum levels of homocysteine (Hcy) ,folate acid (FA) and vita‐min B12 (VitB12 ) with cerebral infarction .Methods The enzymatic cycling method and chemiluminescence immunoassay were used to detect the serum Hcy ,FA and Vit B12 levels in 60 patients with cerebral infarction ,and then the detection results were compared the results of 40 people undergoing physical examination in the same period .Results The serum Hcy level in the cerebral infarction group was (23 .16 ± 8 .17)μmol/L ,which was significantly higher than (8 .09 ± 2 .45)μmol/L in the control group(P<0 .01) ,and the serum FA and Vit B12 levels in the cerebral infarction group were (6 .12 ± 2 .19) ng/mL and (323 .92 ± 133 .79)μmol/L ,which were in the control group lower than (10 .57 ± 5 .70) ng/mL and (530 .85 ± 168 .56)μmol/L in the control group (P<0 .01) .The serum Hcy level in the cerebral infarction group showed the negative correlation with FA and VitB 12 levels ( r1 = - 0 .444 , r2 = -0 .535 ,P<0 .05) .Conclusion High hyperhomocysteinemia is an independent risk factor of cerebral infarction and is related to the decrease of FA and VitB12 .

Objective To establish a HPLC method for determination of hypoxanthine and xanthine in Syngnathus.Methods A Lichrosper C_(18) Column was used.The mobile phase was methanol-0.1%HAc.Walvelength was 254nm.Results The linear range of hypoxanthine was within 5.91～94.56?g?mL~(-1),r=0.9999,sample recovery rate was 99.22%,RSD=(1.25)%.The linear range of Xanthine was within 2.04~32.64?g?mL~(-1),r=0.9998,sample recovery rate was 98.05%,RSD=1.21%.Conclusion This method has good repeatability and flexibility.It can be used for quality control in production of Syngnathus.

Objective To discuss the change of bone resorption of primary nephrotic syndrome (PNS) patients with prednisone treatment, andα-D3 effects on it. Methods ①30 PNS patients diagnosed and 28 healthy people in our hospital were selected;blood and urine samples before and after enough prednisone (pred) treatment were collect-ed to detect the iPTH, calcium and urinary DPD. ②30 PNS patients after 6 weeks enough prednisone treatment were randomly divided into Pred +α-D3 group and Pred group. Results ① PNS group compared with healthy group, urine DPD excretion rate was significantly increased(P<0.05),and blood iPTH was significantly increased (P<0.01);blood calcium was similar to healthy group. ② PNS patient after treatment with pred compared with that before treatment, urine DPD excretion rate was significantly increased(P<0.01),and blood iPTH was signifi-cantly increased(P<0.01). There was no obvious change in blood calcium.③Pred+α-D3 group compared with Pred group, urine DPD excretion rate was significantly decreased(P<0.01),and blood iPTH was significantly de-creased(P<0.05). There was no obvious change in blood calcium. Conclusion α-D3 can effectively reduce bone resorption in PNS patients.

Objective: To analyze the constitution of hospitalization expenses and provide evidence for controlling unreasonable increase of medical costs. Methods: To compare the medical expense constitution of cerebral infarction patients with and without medical insurance; interview the doctors, patients and the medical insurance managers of medical insurance, analyze the influencing factors of the expense through combining the result of interview and quantitative data. Results: The patients with medical insurance spent higher expense than their counterparts, the per capita cost of medical insurance increase by year. Through analyzing the constitution of hospitalization cost, medicine fee and examination cost are the major medical costs. Conclusion: The costs of patients with medical insurance have over-treatment problem, which could be controlled by changing payment method and strengthening management.

A questionnaire survey on the awareness and knowledge about stroke was conducted in 392 residents of Fangzhuang community.The results showed residents in Fangzhuang had high awareness rate about stroke,the rates of knowing early symptoms of stroke were from 57.91％ to 79.08％.83.16％ (326) of residents knew to dial 120 or 999 at emergency.The community doctors were thought as the main resource of gaining the knowledge of stroke (78.32％ ).The results indicate that Fangzhuang community health service center plays an important role in health education and guidance for emergent management of stroke in the community.

Objectives Discuss free fibula flap apply in mandibular defect reconstruction after oval cavity tumors resection.Methods Retrospectively review 15 cases clinical materials who undertake free fibula flap reconstruct mandibular defection.Madibular meloblastoma 5 cases,madibular fibrosarcoma 2 cases,submadibular gland adenoidcyst carcinoma 3 cases,submadibular gland mucoepidermoid carcinoma 2cases,oral floor high differentiated squamous carcinoma 3 cases.Simple fibular flap 10 cases,comples fibular osteocutaneous flap 5 cases.Results Fourteen cases fibular flap survival and grow well,1 case putrescence due to crisis of circulation postoperation.Conclusion Free fibular flap reconstruction madibular defect can recover patients visage and oral function,improve their survival quality.

To study an improved translabyrithine approach, twenty adult cadaver heads (40 sides) fixed with formalin were dissected by mimicking translabyrithine method under light microscope ,and measurements were performed . The results showed that the rate of meeting with mastoid emissary was 33/40(82. 50%). The distance between the middle part of sigrnoid and the vertical part of facial nerve was 7. 22 ?1.95 mm. The width of the middle part of sigmoid was 10. 5 ? 0. 79mm, the left side was 8. 89 ? 1. 03mm, and the right side was 9. 88 ?2.14mm. The height of jugular bulb was 8. 08?3. 46mm,the left side was 6. 78 ? 3. 17mm,and the right side was 9. 07?3. 04mm. The distance between the highest point of jugular bulb and the base of internal acoustic meatus was 4. 54 ? 2. 81 mm, the left side was 5. 93?3. 12mm, and the right side was 2. 65?1. 87 mm. The distance between the inferior margin of internal acoustic meatus aperture and superrior petrosal sinus was 5. 09?1. 29mm . The diameter of superrior petrosal sinus was 1. 43?0. 08 mm. There were two types of superrior petrosal sinus entering to the sigmoid (or transverse sinus). The angle between superrior petrosal sinus and sigmoid was 52. 17 ?6. 93?. The results suggest that the improved translabyrithine approach can give enough vision for cerebellopontine angle operation.

In order to verify the dependability of 3D data, twenty-four temporal bones of adult head specimen fixed with formaldehyde were scanned by multi-slice helical CT. The technique of shade surface display and volume rendering were used in reconstruction. The 3D data of the surface structure of temporal bone were compared with anatomical specimens. The result showed that the new type CT has more rapid speed in 3D reconstruction and clearer imaging. The technique of shade surface display and volume rendering can give very clear structure of temporal bone. The 3D data makes no difference with anatomical measurement (no statistic difference). It is suggested that the 3d data can instruct the clinic directly.

Background Epipolis laser in situ keratomihusis(Epi-LASIK) is an potential surgery for myopia because it synthesize advantages of LASEK and LASIK. But its clinical effectiveness and safeness is remarkable.Objective This study was to evaluate the clinical curative effects and safeness of Epi-LASIK for myopia in different diopter of population. Methods Retrospective study was designed for 208 eyes of 104 patients who underwent EpiLASIK for the correction of myopia. The patients were divided into two groups according to preoperative diopter:lower myopia group( ≤ -6.00 D, 111 eyes) and high myopia group ( ＞-6.00 D,97 eyes). The time of epithelial healing, postoperative syndrome, postoperative visual acuity, refraction, intraocular pressure (IOP) , the safe index, the efficacy index and haze were evaluated at 1,3,6,12 months postoperatively. The written informed consent was obtained from each individual before surgery. Results After operation, 20 eyes ( 18.02% ) of lower myopia group and 29 eyes (29.90%) of high myopia group had obvious pain, presenting statistically significant difference between two groups (X2 = 4. 060, P＜0.05 ). The mean time of epithelial healing was (5.49±0. 83 )days in lower myopia group and (5.85± 0.68 )days in high myopia group with a delayed epithelial healing time in high myopia group( u= 3. 377 ,P＜0.05 ).One year after the treatment,the uncorrected visual acuity( UCVA ) ≥ 1. 0 was 90. 99% ( 101 eyes) in lower myopia group and 75.26% ( 73 eyes) in high myopia group, and 9.91% ( 11 eyes) and 15.46% ( 16 eyes) of the eyes improved byl line or more in best spectacle corrected visual acuity(BCVA) ;whereas 3.60% (4 eyes)and 6. 18% (6 eyes)lost a line or more. 91.89% ( 102 eyes) and 85.57% (83 eyes) in both groups gained within 1.00 D of the attempted correction. The safety index and efficacy index were 1.04 and 0. 98 in lower myopia group,and 1. 01 and 0. 96 in high myopia group without statistically significant difference( P＞0. 05 ). haze occurred in 6 eyes in lower myopia group and 9 eyes in high myopia group and the difference in haze grading was not statistically significant between two groups ( P＞0. 05 ). The high intraocular pressure appeared in 10 eyes and 9 eyes in low myopia group and high myopia group respectively during the fellow up duration and back to normal after topical use of timolol. Conclusion Epi-LASIK is an effective and safe method for correction of different diopters of myopia because of its mild symptom and lower incidence of haze.

Objective To study the correlation between senile mild cognitive impairment (MCI) and metabolize of oxygen-derived free radicals, acetylcholinesterase(AchE), blood lipids and inflammatory mediator. Methods Memory,the levels of superoxide dismutase (SOD), malondialdehyde (MDA), AchE, blood lipids, IL-1? and IL-6 of MIC group(45 cases) and elder healthy group(45 controls) were measured, funther compare and correlative analysis were made.Results Compared with elder healthy group, memory score in each item and memory quotient in MCI group were significantly lower (allP