A total of one hundred and sixty rats were fed with isothiocyanatelabelled candida albicans and then randomly divided into four groups: simple burn (30% TBSA full-thickness scald burn was created on the back), simple antibiotic therapy (oxacillin combind with gentamicin were injected intraperitineally), 30% full-thickness burn plus antibiotic therapy and the control group. The animals were sacrificed and examined at different time phases. The labelled candida albicans were traced in the mesentery lymph nodes, liver, spleen, kidney, lung, blood stream and urine. The labelled candida albicans could be demontrated from 30% of visceral organs at one hour and from 70% at six hours after burn plus antibiotic therapy, while the mesentery lymph node and liver were the primary organs invaded. The process of candida translocation through the intestinal wall was displayed as adhesion and then invasion. For comprative study of the methods or the early diagnosis of systemic dissemination of candida albicans, blood cultures, urine cultures and direct examination of urine under microscope were performed. The detectable rate was only 8% in blood culture and 21.8% in urine culture, however, it was 40% in direct examination of urine, the later method is therefore much more sensitive than the other two.

OBJECTIVE: To study the drug resistance of pseudomonas aeruginosa so as to provide references for rational use of antibiotics in the clinic.METHODS: The clinical distribution and the susceptibility test results of pseudomonas aeruginosa isolated from Beijing Tiantan Hospital in 2006 were analyzed retrospectively.RESULTS: The resistance rate of 334 strains to imipenem stood at 39.8%.The pseudomonas aeruginosa strains were mainly isolated from three important departments(neurological,respiratory and neurosurgery),the resistance rates to imipenem in the above three departments in descending order were as follows: department of respiratory(48.3%),department of neurological(40.2%),and neurosurgery(17.0%).CONCLUSION: The Pseudomonas aeruginosa isolated from Beijing Tiantan Hospital were highly resist to imipenem reduced and the strains isolated from different department varied in bacterial resistance,suggesting the drug resistance of antimicrobial surveillance should be emphasized in some key departments to keep the drug resistance under control.

This paper introduces the operational principle and structure of laser camera. The adjustment of image gray- scale and density is also mentioned

This paper discusses the limitations of traditional evaluation standard for digital medical images, and points out the reasons to select DQE as the new one. The differences of the two standards are compared and analyzed.

Objective:To observe influence ofβreceptor blocker on heart rate variability (HRV)after military train-ing in soldiers.Methods:A total of 80 soldiers with heart rate>60 beats/min from a group army were selected,and randomly divided into metoprolol group (n=40)and control group (n=40).Metoprolol group received metoprolol succinate (12.5mg,once/d)continuously before exercise 5d,while control group received placebo of same dose, 5min dynamic electrocardiography was used to analyze HRV.Heart rate,blood pressure and HRV changes were compared between two groups before and after 5km running.Results:There were no significant difference in heart rate,blood pressure and HRV indexes between two groups before exercise;compared with control group after exer-cise,heart rate and blood pressure were lower,and there were significant reductions in standard deviation of normal to normal RR intervals calculated over the 24 h period [SDNN,(80.1±9.5)ms vs.(69.4±10.5)ms],standard deviation of normal to normal RR intervals in all 5 min segments of the entire recording [SDANN,(65.7±7.8)ms vs.(60.1±9.2)ms],SDNN index [(25.1±11.3)ms vs.(20.4±12.3)ms],root-mean square of differences be-tween successive normal to normal intervals [rMSSD,(25.2±10.7)ms vs.(19.9±11.3)ms],very low frequency [VLF,(451.1±312.9)ms2 vs.(408.3±299.8)ms2 ],low frequency [LF,(191.0±79.3)ms2 vs.(148.2±77.4) ms2]and high frequency [HF,(69.6±50.1)ms2 vs.(51.8±52.7)ms2]in metoprolol group,P<0.05 all.Con-clusion:βreceptor blocker usage before exersise can reduce heart rate variability change after military training,and is helpful to decrease occurrence of acute cardiovascular events.

Objective To explore the possible mechanism of acute cerebral hemorrhage with systemic inflammatory response syndrome (SIRS) which caused multiple organ dysfunction syndrome (MODS), and to investigate the significance of serum nitric oxide (NO) and nitric oxide synthase (NOS) in the development of acute cerebral hemorrhage from SIRS to MODS.Methods The incidence rate of acute cerebral hemorrhage leading to SIRS and MODS was investigated. Serum NO and NOS levels in 73 patients with acute cerebral hemorrhage were detected dynamically by nitratase and chromometry methods. 20 normal healthy people were adopted as control group.Results The incidence rate of cerebral hemorrhage accompanied with SIRS was 47.95%(35/73),74.29%(26/35) of which leaded to MODS. 73 patients serum NO and NOS levels were elevated obviously, and the differences were significant compared with control group (all P

Objective To study X-ray and CT diagnosis and Differential diagnosis of nonossifying fibroma.Methods 29 cases of nonossifying fibroma proved by pathology were included in this paper.29 cases underwent radiography and 12 cases underwent CT scan.There were 22 male and 7 female,with age ranged from 5~59 years(mean age 21.3 years),X-ray and CT findings of tumors were analysed.Results The tumors were located in long bone in 27 cases,in which 17 cases were located in metaphses,2 cases in diaphyses,8 cases in bone end and 2 cases in irregular bone.The maximum diameter of tumors ranged from 0.5~7 cm.The tumors were round,oral or mild lobular in 21 cases,the tumors were showed sclerotic borders in 26 cases.Septum was demonstrated in 22 cases on X-ray plain films,but only 2 cases on CT.Bone expansion was showed in 13 cases.Conclusion Most of nonssifying fibroma have characteristic X-ray and CT findings,the diagnosis be identified by X-ray and CT before operation.

Periodontitis is a chronic infective disease characterized as the destruction of the supporting tissues of the teeth. Bone marrow mesenchymal stem cells, which are ideal adult stem cells for the regeneration of supporting tissues, may play important roles in restoring the structure and function of the periodontium and in promoting the treatment of periodontal disease. As a consequence, the characteristics, especially osteogenic differentiation mechanism, of these stem cells have been extensively investigated. The regulation of the physiological behavior of these stem cells is associated with BMAL1 gene. This gene is a potential treatment target for periodontal disease, although the specific mechanism remains inconclusive. This study aimed to describe the characteristics of BMAL1 gene and its ability to regulate the osteogenic differentiation of stem cells.
ARNTL Transcription Factors ; genetics ; Adult ; Adult Stem Cells ; Bone Marrow Cells ; physiology ; Cell Differentiation ; Hematopoietic Stem Cells ; Humans ; Mesenchymal Stromal Cells ; physiology ; Osteogenesis ; physiology ; Periodontal Ligament ; Periodontitis ; Periodontium ; Regeneration ; Tooth

Objective To investigate the derivatives of ginsenoside sapogenins and their activities against HL-60 cells.Methods The total ginsenoside extract was treated by Smith degradation.All products were isolated by silica gel chromatography and purified by preparative HPLC.On the basis of 1D and 2D NMR data,structures were elucidated.The activity against HL-60 cells was measured by MTT method.Results Three compounds were isolated and identified as 20(S)-protopanaxadiol(PD),20(S)-protopanaxatriol(PT),and 24,25-en-3?,6?-dihydroxy-12,20-(1′-hydroxy) ethanedioxy-dammarane(1).Conclusion Compound 1,named 1′-hydroxy ethanedioxy PT,is a novel derivate of sapogenin with higher inhibitory activity against HL-60 cells than PD and PT.

Objective To explore the relevance of safety culture in hospital nursing and the head nurse leadership behavior.Method The study adopts the method of convenience sampling to diversify the head nurse leadership behavior and nursing safety culture of 344 nurses in 3 first-class tertiary hospital of traditional Chinese Medicine in Heilongjiang Province.All survey data were entered into the SPSS17.0 software for analysis.Finally,conclusion was got.Result The head nurse leadership behavior is the highest score for leadership charm,followed by passive exception management,personalized care,active exception management,charisma,contingent reward and intellectual stimulation.Safety culture of nursing is slightly higher than medium level,in wh ch the team atmosphere,the satisfaction of the work,the unit security atmosphere,the management experience is higher than the medium level.The cognitive score of the pressure is lower than the medium level.Conclusion All dimensions of nurses' perception of head nurse leadership behavior has a positive correlation with each dimension of safety culture in hospital nursing.