AIM: To study the changes of hippocampal ne uronal microenvironment and alterations of excitatory amino (EAA) and inhibitory amino acid (IAA) in extracellular fluid (ECF) in different time after t hrombotic cerebral ischemia in tree shrews. METHODS: The model of focal thrombotic cerebral ischemia was ind uced by photochemistry-technology in tree shrews. Hippocampal ECF was collected b y microperfusion. pH, PCO 2, PO 2 and HCO 3- were analyzed by blood gas ana lyzer, and Asp, Glu, Gly and GABA were measured by high-performance liquid chrom atography (HPLC)-PITC technology after occlusion. RESULTS: The contents of Asp, Glu, Gly and GABA in hippocampal E CF increased, and pH, PO 2 and HCO 3- decreased after photochemical induced cerebral ischemia in tree shrews. There were significant differences between ish emic group and sham group (P

The complete length of P gene from rabies virus was amplified by RT-PCR using a pair of specific primers designed according to the relevant sequences from GenBank. The PCR product was cloned into cloning expression vestor pGM-T to obtain the cloning expressed plasmid pGM-T-P. After double-digestion by NotI and EcoRI, the product was transferred into prokaryotic expression vetor pET-32a(+)to obtain the prokaryotically expressed plasmid pET-32a-P. The target gene was then expressed in the E.coli BL21(DE3) cell with IPTG induction. The highest expression of target protein was analysed by SDS-PAGE, and the good immunoreactivity to rabies virus antibodies was proved by Western-blot analysis. By using purified protein, the indirect ELISA assay for the detection of rabies virus antibodies in canine serum was applied after management of the optional working condition.

Objective To summarize the experience of pituitary adenoma resection by single-nostril transsphenoidal approach and analyze the indications,intraoperative and postoperative common problems in order to improve the technique and quality of life.Methods Summarize the clinical data of 611 pituitary adenoma resections by single-nostril transsphenoidal approach from January 2005 to June 2010 retrospectively,reanalyze the choice of operative indications,analyze the bleeding during the operation and sums up the relevant pro cessing suggestions,and summarize the reasons and countermeasures of postoperative visual impairment.Results The tumors were totally removed in 538 cases,sub-resections in 59 cases,sub-totally removed in 14 cases,and no deaths.Eleven cases were poor gasification or concha sphenoid sinus in 11 totally removed patients.The bleeding came from diploe,emissarium,mucosa of sphenoid sinus,diploe,epidural space,dura matter of the sella turcica region,intercavernous sinuses,tumor or the tumor bed.The bleeding was controlled effectively with different approaches.There were 3 patients who underwent visual impairment,and the impairment was improved after treatment.Conclusion Following the correct operation principle,we can improve the rate of total resection; the single-nostril transsphenoidal approach is also suitable for the poor gasification; The prognosis can improve after deal with the intraoperative and postoperative common problems.

ObjectiveTo compare the therapeutic effect of mininal]y invasive removal of intracranial hematoma and conservative treatment in curing hypertension cerebral hemorrhage of elderly patients.Methods60 patients with hypertension cerebral hemorrhage were divided into two groups.Group A were adopted minimally invasive removal of intracranial hematoma(YL-1 type puncture needle was used into the hematoma edge,and sucked slowly the hematoma), and group B were adopted medical treatment(antihypertensive drugs, dehydration, free radicals and nutrition conservative treatment) ,then clinical therapeutic effect and activity of daily living were observed.ResultsThe hematoma evacuation time in group A (4.5 ± 1.2) d is shorter than in group B (17.0 ± 1.9) d (t = 6.04 ,P ＜ 0.05) ; The rate of neurological function recovery in group A (66.7％) is higher than in group B(93.3％) (x2 = 5.46,P ＜0.05) ; The activity of daily living in group A (80.0％) was better than group B (50.0％) (x2 = 5.93, P ＜ 0.05).The complication rate in group A (30.0％) is lower than in group B (66.7％) (x2 = 8.08, P ＜ 0.05).ConclusionThe therapeutic effect of minimally invasive removal of intracranial hematoma in curing hypertension cerebral hemorrhage was better than medical treatment.

Objective To investigate the effect of ketamine anesthesia in the early pregnancy on the c-fos mRNA and c-jun mRNA expression in the offsprings of rats. Methods Thirty pregnant SD rats at 5-13 days of gestation were randomly divided into control group and ketamine group (n = 15 each). Ketamine 20 mg/kg was injected intravenously through tail vein followed by 2 h infusion at a rate of 130 mg·kg-1 ·h-1 in ketmine group.While the equal volume of normal saline was given instead of ketamine in control group. The learning and memory function of the offsprings were tested by Morris water maze test on postnatal day 20 and 30. The hippocampal tissues were taken to detect the expression of c-fos mRNA and c-jun mRNA and to observe the ultrastructure. Results Compared with group C, the escape latency was significantly prolonged at 2 days during the test which was performed on postnatal day 30, but there was no significant difference in the expression of c-fos mRNA and c-jun mRNA on postnatal day 20 and 30 and in the indices mentioned above on postnatal day 20 in ketamine group (P ＞0.05). The damage to hippocampal neurons happened in ketamine group. Conclusion The mechanism by which ketamine anesthesia in the early pregnancy inhibits the cognitive function of the offsprings is related to the hippocampal neuron damage, but not related to the expression of c-fos mRNA and c-jun mRNA in hippocampus.

Academe hash't got the same view about science attribute of traditional Chinese medicine(TCM)up to now.This article demonstrates the scientific attribute of the traditional Chinese medicine from the nature of science,the seientific attribute of medical science and the relationship between the traditional Chinese medicine and medical science.TCM needs development.In order to develop the traditional Chinese medicine quickly,on the basis of inheriting tradition,we should make the best use of modem scientific technology.Not simply copy the theories and methods of western medicine but using the scientific measurement to build up the measurement of TCM symptoms,the relationship between symptoms and the change of the body,explore the process of physiology and pathology,and combine with western medicine to develop a mature science-medical science.

Aim To explore the effect and mechanism of atorvastatin on lipopolysacchairde (LPS) inducing the expression of inhibitor of nuclear factor ?B? (I?B?) in human vascular endotheliar cells. Methods The human vascular endotheliar cell line ECV304 was cultured and divided into five groups as control group, LPS group, and low, moderate or high does atorvastatin groups. After incubated with different densities atorvastatin, the three atorvastatin groups and LPS group were stimulated with LPS 30min. Then the activation of I?B? was observed with immnofluorescence. The proteins expressions of I?B? and phosphorylated I?B? were detected with western blot. The ex-pression of I?B? mRNA was examined with reversetranscription-polymerase chain reaction. Results Atorvastatin could inhibit the translocation of p65 to the nucleus and reduce the phosphorylation and degradation of I?B? in a dose-dependent manner. The high density atorvastatin could increase the expression of I?B? mRNA. Conclusion The atorvastatin can inhibit the activation of nuclear factor ?B by regulating the expression and degradation of I?B?.

OBJECTIVETo observe thee efficacy of different ways of percutaneous coronary intervention (PCI) for treating elderly coronary heart disease (CHD) patients.

METHODSTotally 470 elderly CHD patients were classified to three age brackets (equal to or more than 85 years old, 60 to 74 years old, 75 to 84 years old). They were assigned to the transradial intervention (TRI) group (236 cases) and the transfemoral intervention (TFI) group (234 cases) according to different intervention pathways. Correlated indices and postoperative clinical efficacy were compared between the two groups.

RESULTSA higher successful rate of surgery was obviously got in patients 85 years old or older than 85 than in those 60 to 74 years old and 75 to 84 years old (P <0. 05). The incidence of major cardiovascular events (MACE) was reduced at post-operative 12 and 24 months in patients 85 years old or older than 85 (P <0. 05). The case number for changing intervention pathway were increased in the TRI group with statistical difference (P <0. 05). Compared with the TFI group, the case number for changing intervention pathway was increased; the time for arteriopuncture, the time for catheterization, and the time for X-ray exposure were prolonged; the time for postoperative bedding were obviously shortened; the incidence of vascular complications at the puncture site were lowered. The incidence of postoperative 12-month MACE was lowered, all with statistical difference (all P <0. 05). The incidence of MACE within postoperative 24-month MACE decreased in patients 60 to 74 years old and 75 to 84 years old (P <0. 05). The incidence of MACE within postoperative 24 months increased in patients 85 years old or older than 85 of the TRI group with statistical difference (P <0. 05).

CONCLUSIONTRI can be preferably chosen for PC in treating elderly CHD patients.

Aged ; Aged, 80 and over ; Coronary Artery Disease ; surgery ; Female ; Humans ; Incidence ; Male ; Middle Aged ; Myocardial Infarction ; Percutaneous Coronary Intervention ; methods ; Radial Artery ; Treatment Outcome

Adolescent ; Child ; Child, Preschool ; Female ; Homeodomain Proteins ; genetics ; Humans ; Infant ; Male ; Oncogene Proteins, Fusion ; genetics ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; genetics

Objective To observe the effect which the skeletal muscle satellite cells were transplanted on denervated skeletal muscle atrophy so as to provide the experimental data for treating denervated muscle atrophy in vivo. Methods Thirty-two Sprague-Dawley rats weighting 200-250 g were randomly divided into control group and experimental group. Each group included 16 rats, the animal model of denervated gastrocnemius muscles were formed by cutting the right sciatic nerve of rats caused nerve despair about 1 cm. Muscle satellite cells were obtained from the dorsal and lower exterenity muscle of SD rats. Before transplantation, muscle satellite cells being labeled with DAPI (4′-6-Diamidino-2-Phenylin Dole) in vitro. Muscle satellite cells and NS were implanted into denervated skeletal muscle from the two groups. Bilateral gastrocnemius muscles of each rat from the two groups were taken and weighed at second and eighth post-operative weeks respectively. The above muscles underwent anti-actin immunohistochemical and HE staining and muscle cross-sectional area of fiber and the actin content of the rats were measured by the image analysis, the data of which was handled with SPSS software. Results The satellite cells and myofiber with fluorescence were observed in transplantation site. The group of skeletal muscle satellite cells transplanted when two weeks and eight weeks denervated gastrocnemius muscle wet weight remnant rate and muscle cross-sectional areas of fiber remnant rate and muscle actin content were better than in the control group respectively(P