Adenoviridae ; genetics ; Genes, p53 ; Genetic Therapy ; methods ; Genetic Vectors ; Humans ; Neoplasms ; therapy ; RNA Interference ; Tumor Suppressor Protein p53 ; genetics

Cardiomyopathy, Restrictive ; diagnosis ; surgery ; Diagnosis, Differential ; Humans ; Infant ; Male

Objective: To study the defense style of undergraduates with cluster B personality disorders. Methods:26 undergraduates with cluster B personality disorders were diagnosed by PDQ~ +4 and PDI from 2826 undergraduates, then they and 63 normal undergraduates were assessed with DSQ (defensive style questionnaire). Results: ①The incidence of histrionic, narcissistic, borderline and antisocial personality disorders were 0.25%?0.50%?0.39% and 0.11% .②The undergraduates with B cluster personality disorder had higher scores in immature defenses and idealization, omnipotenic-devaluation and isolation than normal control (t=3.18-7.03)(P

Objective To explore clinical effect of modified postural drainage for treating pulmonary infection of senile patients with gastric cancer.Methods 107 cases of patients with gastric cancer were randomly divided into two groups:control group (n =53) and treatment group (n =54),which were respectively treated by routine postoperative therapy and nursing measures and modified postural drainage based on these routine measures,and compared daily amount of expectoration sputum,positive cases of sputum culture,restore time of postoperative blood test,postoperative fever time,cases of pulmonary infection on the postoperative tenth day and length of stay.Results Daily amount of expectoration sputum of patients in treatment group was significantly higher than that of control group [(185.7 ± 23.7) ml vs.(99.7 ± 17.6) ml] (P ＜ 0.05).However,restore time of postoperative characters of blood test [white blood cell count (2.9±0.9) d vs (5.0±0.7)d,C-reaction protein (35±0.7)d vs.(7.4±0.6) d],postoperative fever time [(1.9±0.5) d vs.(3.6±1.4) d],cases of atelectasis (5 cases vs.19 cases) and length of stay [(1.9±0.5) d vs.(3.6±1.4) d] were respectively significantly less than those of control group (P ＜ 0.05).Conclusions Modified postural drainage could effectively prevent postoperative atelectasis and pulmonary infection,significantly reduced incidence of postoperative atelectasis of senile patients with gastric cancer operation,and so it was worthy of clinical application.

Objective To explore the effect of lipo-prostaglandin E1 plus dextran 40 in the prophylaxis of hepatic veno-occlusive disease(HVOD) after hematopoietic stem cell(HSC) transplantation.Methods Two hundred and thirty-four patients,162 males and 72 females,mean age 30.6(ranged from 3 to 64),were admitted during May 1999 to April 2007 and undergone HSC transplantation.Lipo-prostaglandin E1 associated with dextran 40 were administered to all patients for the prophylaxis of hepatic veno-occlusive disease.Lipo-prostaglandin E1,10?g dissolved in 100ml saline,20?g per day iv,were administered from day 7 before transplantation(-7 day) to day 30 after transplantation(+30 day).Dextran 40,500ml per day iv,were administered from-1 day to +5 day.In order to identify the risk factors inducing HVOD,patients were allocated to 2 groups according to following factors:age(

Objectives To evaluate the effectiveness of transcarotid artery chemotherapy for gliomas after surgery, and selection of drug, avenue of administration, and optional time for the therapy. Methods Beginning from 4 to 30 days after operation, Nimustine (ACNU, Japan) 2.5mg/kg was injected per carotid artery, once every week for three times as one course. A second course of treatment was given after an interval of 4 to 6 weeks. Results With the above regime, the effect was marked in 39 cases (18.8%), fairly effective in 44 cases (20.8%), only slightly effective in 59 cases (27.8%), no effect in 61 cases (28.8%), and failure in 5 cases (2.4%), the mean survival time was nearly 100 weeks. Conclusion Transcarotid artery chemotherapy for gliomas is helpful in prolonging survival period, with little side effects, easy to carry out, less expensive, and better accepted by the patients.

Objective To compare the clinical results of choledocholithotomy by laparoscopic surgery and open surgery.Methods One hundred and Sixty-nine patients with cholecystoithiasis combined with choledocholithiasis were divided into laparoscopic surgery group(85 cases of laparoscopic choledocholithotomy,LCD-TD) and open surgery group(84 cases of open choledocholithotomy,OCH-TD).The clinical data in the two groups were compared and analyzed.Results The amount of intraoperative bleeding,the recover time of intestinal peristalsis after treatment,length of hospital stay,cases of postoperative pain and biliary fistulas in laparoscopic surgery group were evidently better than those of open surgery group(all P

Objective:To explore effect of relevant training on understanding of theory of naive biology for plant reproduction in 5- and 6-years old,and to attempt to provide demonstration for a debate of different views between domain universality and domain specificity cognitive development.Methods:48 preschoolers who did not comprehend concept of plant reproduction screened from 64 preschoolers ages 5 to 6 were surveyed by clinical method.Half of the 48 children were assigned to intervention group,the other to control group.The effects of intervention were compared by the experimental before-after design.Results:Relevant biological knowledge might widely accelerate 5- and 6-year-olds' understandings for plant reproduction,and 6-year-olds had more enhancement than 5-year-olds.Pass rates of intervention group were more 29.2% than control group in 5 year olds,and more 54.1% in 6 year olds.Conclusion:Acquiring specific knowledge will generally improve preschoolers' cognition on plant reproduction.Children' cognitive development is influenced jointly by both domain-specific and domain-general thinking.

Objective： To study the role of mechanical stress on osteoblast-like characteristics expressed by human periodontal ligament cells in vitro. Methods： Human periodontal ligament cells (hPDLC) were cultured by explant method. We investigated the gene expression for osteoblast-like marker proteins in periodontal ligament stimulated by cyclical mechanical force in vitro by in situ hybridization with non-radioisotopic oligonucleotide probe for alkline phosphatase (ALP) and osteocalcin (OCN). Results： Human periodontal ligament cells exhibited a stronger signal for ALP mRNA stimulated by cyclical mechanical force, and exhibited a moderately intense positive signal for OCN mRNA—OCN was a marker of differentiated osteoblast-like cells. Conclusion： These findings suggest that mechanical force plays role in differentiation from hPDLC to osteoblast-like cells.

Objective To compare the clinical outcomes and safety of synchronous vs staged bilateral total hip arthroplasty (THA).Methods Fifty-eight cases (116 hip joints) undergone bilateral THA from January 2008 to January 2010 were reviewed retrospectively.Follow-up period was postoperative 2 years,including synchronous bilateral THA in 29 cases (synchronous group) and staged bilateral THA in 29 cases (staged group).The two patient groups were compared in aspects of total operation time,total amount of blood loss,total amount of blood transfusion,duration and cost of hospitalization,postoperative discrepancy in bilateral leg length,preoperative and postoperative function score and perioperative complications.Results During the follow-up,no complications of incision infection,deep vein thrombosis and prothesis dislocation or loosening were noted.And postoperative delirium symptoms occurred only in four cases (three in synchronous group and one in staged group).There were no statistical differences between synchronous group and staged group in aspects of total operating time [(117.9 ± 23.8) minutes vs (124.1 ± 18.8) minutes],total amount of blood loss [338.1 ml (180-720 ml) vs 303.9 ml (200-600 rnl)],total amount of blood transfusion [227.6 ml (0-800 ml) vs 189.7 ml (0-400 ml)],postoperative function score.However,differences of the following data were considered statistical significance between synchronous group and staged group:expense of hospitalization [9.5 ten thousand yuan (7.0-11.3 ten thousand yuan) vs 10.5 ten thousand yuan (8.8-11.0 ten thousand yuan)],length of hospitalization [(12.1 ±3.2) days vs (20.1 ±3.5) days],postoperative discrepancy in bilateral leg length [(0.11 ± 0.22) cm vs (0.42 ± 0.44) cm].Conclusions Synchronous bilateral THA is safe and feasible as far as the patients' physical condition is allowed,perioperative management is rational and physicians have mature surgical techniques.Moreover,it gains advantage over staged bilateral THA considering cost of hospitalization,length of hospitalization and postoperative discrepancy in bilateral leg.