Animals ; Cytidine ; analogs & derivatives ; pharmacology ; DNA Methylation ; drug effects ; DNA Modification Methylases ; metabolism ; Gene Knockout Techniques ; Histone Acetyltransferases ; metabolism ; Histone Deacetylase Inhibitors ; pharmacology ; Humans ; Hydroxamic Acids ; pharmacology ; Neoplasms ; genetics ; physiopathology ; therapy ; RNA Interference ; Valproic Acid ; pharmacology

Objective To determine the transfection of PTEN gene in human ovarian carcinoma SKOV3 cells by ultrasound mediating and contrast agent′s enhancing, and to explore the mechanism of PTEN gene inhibiting the tumor cells′ invasion. Methods After addition of PTEN gene and 5 ?l lipofectamine,human ovarian carcinoma SKOV3 cells on 6-well plates were explored to ultrasound for 60 s in the presence(200 ?l) of ultrasound agent. The invasiveness of transfected cells was measured quantitatively by Matrigel invasion assays(Transwell chamber) after 48 h. Results The invasiveness of SKOV3 cells transfected by ultrasound-mediated PTEN gene was significantly declined compared with that of the controls. Conclusions The transfection of PTEN gene into SKOV3 cell can inhibit their invasion.The gene transfection can be enhanced by ultrasound and contrast agent,which may be expected to be a new means of clinical gene therapy.

Objective To observe the immunologic function of critically ill newborn and the relative function of nuclear factor kappa B(NF-?B).Methods The critically ill group contained 50 cases,and 25 cases from healthy newborns were used as control group.Blood samples were collected in each case,levels of cytokine interleukin(IL)-4,interferon(IFN)-?,tumor necrosis factor(TNF)-? and NF-?B were detected.Result Compared with control group,NF-?B of the critically ill newborn activated and the cytokine were disorder,and IL-4 and TNF-? increased,but IFN-? decreased.Conclusions Critically ill newborn exist immune functional disorder.Furthermore,NF-?B activation may be involved in the process in infants with critically illness.

Objective To study the effects of modified Sanxiang Decoction on gastrointestinal transmission function of patients with functional bloating (FB). Methods Sixty cases of FB patients were randomly divided into treatment group (40 cases) and control group (20 cases). Treatment group was given modified Sanxiang Decoction, while control group was given placebo granules, one bag each time. Twice a day, taking it after mixing it with water. Gastrointestinal symptoms, 48 h GITT, and adverse reactions were recorded. Results There was statistical significance in TCM symptom scores between before and after treatment in the two groups (P<0.05). After treatment, the total score of abdominal distension, belching, anorexia and TCM symptoms of the treatment group were lower than the control group, with statistical significance (P=0.000, 0.000, 0.010, 0.003). The total effective rate was 82%(33/40) in the treatment group, 30%(6/20) in thecontrol group;the treatment group was better than the control group (P<0.05). A total of 30 patients completed gastrointestinal transit test, of which delayed 48 h GITT pass rate accounted for 54% (16/30). Compared with the control group, the 48 h GITT of the treatment group was more obviously improved than that of the control group with statistical significance (P=0.002). There were no significant adverse reactions in both groups. Conclusion Most of FB patients have the problem of abnormal intestinal motility. Modified Sanxiang Decoction can improve the symptoms of patients with FB, promote gastrointestinal motility, and improve intestinal transmission function.

To purify the protein encoding the small capsid protein (SCP) of KSHV and analyze its immunogenicity, the carboxyl terminus of orf65 of Kaposi's sarcoma associated-herpesvirus (KSHV) was expressed in a prokaryotic expression system. The expression of recombinant E. coli containing pQE-80L-orf65 was induced by isopropyl-β-D-thiogalactopyranoside (IPTG) and the fusion protein was purified by chromatography. The expressed protein and its purified product were identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and showed that 9 kDa was the expected size of the purified orf65 protein. The antiserum was produced in rabbit which was immunized by purified orf65 protein. An ELISA assay was established to analyze the immunogenicity of the purified orf65 protein. The ELISA analysis demonstrated that orf65 protein has strong immune activity, and the immune activity of polyclonal antibody against orf65 was more than 4 fold higher than that in the serum of the non-immunized rabbit. These results demonstrate that purified orf65 protein has very strong immunogenicity and can be used in screening KSHV infection in the general population using ELISA.

Objective To explore the influencing factors of the pregnant women with preeclampsia in blood pressure,glucose and lipid metabolism to hypertension after delivery.Methods One hundred patients of preeclampsias in Obstetrics and Gynecology Department of Maternal and Child Care Sservice Centre of Weinan from April 2012 to May 2014 were selected as the observation group.One hundred maternal-health at the same period were selected as the control group.All observed objects were followed up from May 2014 to May 2015.The general situation,sugar metabolism,lipid metabolism were recorded when in group and folllowed up.The general situation,sugar metabolism,lipid metabolism in prenatal were contrasted in two groups of prenatal and postpartum.Influence factors for hypertension of preeclampsia in pregnant women after childbirth were analyzed.Results Family history of hypertension,history of long-term use of contraceptives,prenatal body mass index(BMI),postpartum BMI,systolic prenatal,antenatal diastolic in the observation group were higher than that in the control group.The differences were statistically significant (P＜ 0.05).The fasting glucose (FPG),fasting insulin antenatal,prenatal insulin resistance index,total cholesterol(TC),triglycerides (TG),low-density lipoprotein cholesterol (LDL-C) of prenatal and postpartum in the observation group were (4.51 ±0.48) mmol/L and (5.21±1.08) mmol/L,(10.11±3.88) mU/L and (9.68±4.97) mU/L,(1.64±0.65) and (2.34±1.48),(6.21±0.98) mmol/L and (5.14±0.51) mmol/L,(3.51±1.35) mmol/L and (1.41 ±0.79) mmol/L,(3.31±0.97) mmol/L and (2.69±0.56) mmol/L,higher than that in the control group ((4.12±0.51) mmol/L and (4.76±0.97) mmol/L,(8.19±2.99) mU/L and (7.25±3.96) mU/L,(1.47 ±0.42) and (1.79±0.79),(5.91± 1.01) mmol/L and (4.99±0.39) mmol/L,(2.96±0.87) mmol/L and (0.86±0.78) mmol/L,(3.01 ± 1.01) mmol/L and (2.46±0.47) mmol/L),the differences were statistically significant(t=5.238,3.690,2.069,2.005,3.225,2.015,2.917,3.600,3.089,2.200,4.661,2.961;P＜0.05).Prenatal and postpartum BMI (OR =1.356,95％ CI 1.012-2.102;OR =1.378,95％ CI 1.034-2.609),fasting blood glucose level of prenatal(OR=1.753,95％CI 2.307-59.257) and postnatal fasting insulin levels(OR =1.351,95％ CI 1.810-112.267),the difference of systolic pressure of prenatal and postnatal(OR=1.247,95％CI 1.208-108.323),the difference between prenatal and postnatal triglycerides (OR =1.119,95％ CI 4.540-77.559),the difference of insulin resistance index (OR =1.529,95％ CI 1.828-92.844) and the difference between high-density lipoprotein cholesterol hypertension (OR =2.547,95％CI 1.763-110.693) were the risk factors that can cause preeclampsia occurs hypertension after childbirth (P ＜0.05).Conclusion Preeclampsia with high blood pressure,abnormal glucose metabolism and lipid metabolism are the risk factors that can cause preeclampsia occurs hypertension after childbirth.

Objective To study the TCM syndrome distribution and characteristics of pregnant patients with cold in Panzhihua region;To provide evidence for prevention and treatment of this disease.Methods Totally 107 pregnant patients with cold were analyzed to obtain TCM syndrome data and summarize the distribution and characteristics of this disease.Results The syndromes of 107 cases of pregnancy cold, from high to low were sore throat (64.49%), thirst (62.62%), nasal congestion (61.68%), cough (59.81%), runny nose (55.15%), and defense phase symptoms such as sore throat, thirst and nasal congestion were obvious. The main TCM syndrome was wind-heat syndrome, accounting for 82.24%. The incidence rates of early pregnancy (<12weeks), midtrimester pregnancy (13-27 weeks), and late pregnancy (>28 weeks) were 32.71% (35/107), 44.86% (48/107), and 22.43% (24/107), respectively. The incidence rates of early pregnancy and midtrimester pregnancy were higher than that of late pregnancy (P<0.05). The incidence rates were 53.27% (57/107) in spring and summer and 46.73% (50/107) in autumn and winter, with statistical significance (P<0.01). The incidence of wind-heat syndrome in spring and summer was 62.5% (55/88),Conclusion The most common syndrome in pregnancy cold was wind-heat syndromes. The most common characteristics were warm, hot and dry. Early or middle pregnant patients were more likely to catch cold in spring and summer. The syndrome distribution and characteristics were relevant to local environment and climate factors.

Coverage of internal iliac artery during endovascular abdominal aortic aneurysm repair may cause ischemic complications in buttock,intestine,lower extremity and genital.We reported nursing care of 72 patients in our center to investigate the key points of nursing.Our experience were as follows:individualized blood pressure management and doctor-nurse cooperation in pain management were key points in preoperative nursing;close postoperative observation was focused on ischemic colitis,buttock claudication and ischemic buttock skin;cooperation with ostomy care group improved pertinency of nursing care;rounding by integration of doctors and nurses obtained high satisfaction and improved patients' compliance.

Pediatric surgery is the important part of the paediatrics,and the complicated content,limited time and many difficulties are the main problems of pediatric surgery teaching.The bed side teaching is the supplement for the class teaching,which could solve those problems.Multi-media technique is used in bed side teaching of Pediatric surgery,which is convenient for preparing lesson and teaching of teachers and review and summary of students.After the technique is used,the teaching efficiency and the quality are obviously improved.