2.Experimental ultrasonographic study of abdominal aorta in diet-induced hyperhomocysteinemia rabbits
Xinghua WANG ; Fen GAO ; Jie LI
Chinese Journal of Ultrasonography 2003;0(07):-
Objective To investigate the relation between serum homocysteine(HCY) and the development of atherosclerosis(AS).Methods Diet contained 1% methionine was fed to make models of hyperhomocysteinemia and atherosclerosis in 18 male New Zealand rabbits. Hyperlipemia group was as control group. Ultrasonographic image was performed in 0,4,8 weeks respectively with HP 8500 and recorded the stenosis and plaque of abdominal aorta compared with pathology.Results Stenosis and plaque of abdominal aorta were observed in 4 weeks in high fat diet group. Stenosis and plaque of abdominal aorta were observed in 8 weeks in high methionine diet group.This results were confirmed by pathology anatomy.Satistically,significant correlation was found between AS and hypercholesterolemia or hyperhomocysteinemia respectively.But there was no correlation between hypercholesterolemia and hyperhomocysteinemia. Conclusions Hyperhomocysteinemia is an independent important risk factor of atherosclerosis.
3.Mucosa-associated lymphoid tissue lymphoma.
Li-ping GONG ; Jie ZHENG ; Zi-fen GAO
Chinese Journal of Pathology 2006;35(4):240-243
Adaptor Proteins, Signal Transducing
;
genetics
;
metabolism
;
B-Cell CLL-Lymphoma 10 Protein
;
Gastrointestinal Neoplasms
;
genetics
;
metabolism
;
pathology
;
Gene Expression Regulation, Neoplastic
;
Humans
;
Lymphoma, B-Cell, Marginal Zone
;
genetics
;
metabolism
;
pathology
;
Oncogene Proteins, Fusion
;
genetics
;
metabolism
;
Translocation, Genetic
4.Investigation and analysis of the present state of antibiotics use in hospitalized children.
Hui-fen ZHANG ; Feng GAO ; Bai-ping LI
Chinese Journal of Pediatrics 2006;44(2):139-141
Anti-Bacterial Agents
;
administration & dosage
;
adverse effects
;
therapeutic use
;
Child
;
Child, Hospitalized
;
statistics & numerical data
;
China
;
Drug Resistance, Bacterial
;
drug effects
;
Drug Therapy, Combination
;
methods
;
Drug Utilization
;
statistics & numerical data
;
Humans
;
Pediatrics
;
Retrospective Studies
5.Application of failure mode and effect analysis in preventing stroke patients from aspiration
Fen LV ; Yuan GAO ; Xia LI ; Xiaoya CHEN
Chinese Journal of Practical Nursing 2013;29(21):33-36
Objective To investigate the application effect of FMEA(failure mode and effect analysis) in prevention of aspiration after stroke.Methods From October 2011 to April 2012 48 stroke patients in our department without implementation of the FMEA management mode were set as the control group.From May 2012 to November 2012,another 48 cases were set as the observation group,in addition to the routine neurological nursing care,we also used FMEA to find forward-looking reason of the failure mode of aspiration and operation process analysis,evaluation,calculation and identify the risk factors of aspiration in patients with cerebral apoplexy and high-risk operation steps,prevention priority action plan and improvement measures.The incidence of aspiration,RPN value of six high risk factors,and satisfaction degree and mastering degree of aspiration related knowledge by patients and their families were compared.Results In the observation group,the crisis values of 6 high risk factors was significantly lower than those of the control group.Significant differences existed in satisfaction degree and mastering degree of aspiration related knowledge by patients and their families as well as hospitalization time.There was no aspiration cases during hospitalization in the observation group while 3 aspiration cases in the control group.Conclusions Application of FMEA to evaluate patients with swallowing disorder after stroke,analyzing and taking relevant measures can effectively prevent risks,reduce the occurrence of aspiration,improve the quality of nursing and promote patients' recovery.
6.Transcatheter closure of coronary artery fistula
Zhiqing YU ; Aiqing ZHOU ; Wei GAO ; Fen LI
Journal of Interventional Radiology 1994;0(03):-
Objective To explore the indication and the methodology of transcatheter closure of coronary artery fistula.Methods Altogether 17 cases for intervention,included 13 using the Cook corp coil(2 detachable and another 11 un-detachable);1 with the Pfm corp detachable coil;3 with the AGA corp Amplatzer-Duct.The other 26 cases underwent surgery. Results Intervention group,showed 3 failure cases because of selecting too small and un-detachable coil in early stage with final displacement.Fourteen cases were successful.Follow-up 3 years showed no residue shunt and other complications.All patients were successful with surgery.Conclusions Transcatheter closure of the coronary artery fistula is mild invasive,short hospitalization,safe,and effective under proper indications.
7.Application of serum alpha-fetoprotein, α-L-fucosidase, 5'-nucleotidase and γ-glutamyltransferase in early diagnosis of hepatocellular carcinoma
Haifeng GAO ; Qiujian ZHAO ; Jing WANG ; Fengying GAO ; Fen QIAO ; Xiaoyan LI
Cancer Research and Clinic 2016;28(8):519-522,527
Objective To investigate the application of alpha-fetoprotein (AFP),α-L-fucosidase(AFU),5'-nucleotidase(5'-NT) and γ-glutamyltransferase (GGT) in diagnosis of hepatocellular carcinoma(HCC),and to provide the basis for its early and rapid diagnosis.Methods The clinical data of 120 cases with initial diagnosis as hepatocellular carcinoma,150 cases with liver cirrhosis,200 cases with hepatitis were analyzed retrospectively from June 2013 to August 2014,meanwhile,100 healthy people were selected as control group.The serum AFP,AFU,5'-NT and GGT levels were measured by electrochemiluminescence immunoassay,rate method,peroxidase method and enzymatic colorimetric assay,respectively.The results were analyzed by receiver operating characteristic (ROC) curve,while the sensitivity,specificity,AUC were calculated at the same time.Results The levels of serum AFP,AFU,5'-NT and GGT were significantly different among HCC group,liver cirrhosis group,hepatitis group and control group (all P<0.05).When tested alone,the sensitivities of AFP,AFU,5'-NT and GGT were 58.33 %(70/120),82.50 %(99/120),85.00 %(102/120),78.33 %(94/120),the specificities were 88.33 %(106/120),73.33 %(88/120),88.33 %(106/120),71.67 %(86/120),and AUC were 0.655,0.702,0.814,0.754.When four indexes were performed with combined test,the sensitivity was 98.33 %(118/120),specificity was 96.67 %(116/120),AUC was 0.975,which were higher than the single detection (all P<0.05).Conclusion The combined detection of serum AFP,AFU,5'-NT and GGT can greatly increase the sensitivity and specificity for diagnosing HCC and will gain mutual complement advantages,which is of great clinical value for early and rapid diagnosis.
8.Trisomy 3 in intestinal B-cell lymphoma of mucosa-associated lymphoid tissue type.
Wei LIU ; Ning LI ; Min LI ; Zi-fen GAO
Chinese Journal of Hematology 2004;25(4):217-219
OBJECTIVETo determine the frequency of trisomy 3 in the intestinal B-cell lymphoma of mucosa associated lymphoid tissue (MALT) type and to explore the relationship between these two.
METHODSParaffin embedded tissue sections of intestinal B-cell lymphomas of MALT type, according to WHO classification, were successfully studied. Seven cases were classical MALT lymphoma, being clinically low-grade, and one MALT lymphoma with large cell transform, clinically high-grade. By using biotin labelled DNA probes specific for chromosome 3 centromeric, chromosome in situ hybridization (CISH) were performed. Probe for C16 was used as the positive reference of the method, and chronic intestinal inflammation as the experiment control for detection of C3 copies in tumor cells.
RESULTSOf the 7 low-grade classical MALT lymphoma, 5 showed trisomy 3 and 2 normal C3. The only case of MALT transformed was trisomy 3. The frequency of trisomy 3 in the intestinal low-grade lymphoma was 71.4%.
CONCLUSIONThe frequency of trisomy 3 in the intestinal low-grade lymphoma is high, indicating that it may be involved in the pathogenesis of this disease and may be of value for the diagnosis.
Adult ; Aged ; Chromosomes, Human, Pair 3 ; Female ; Humans ; Intestinal Neoplasms ; genetics ; Lymphoma, B-Cell, Marginal Zone ; genetics ; Male ; Middle Aged ; Trisomy
9.Catherization in congenital heart diseased patients with stage operation
Zhiqing YU ; Aiqing ZHOU ; Wei GAO ; Fen LI ; Jianping YANG ; Yun LI ;
Journal of Interventional Radiology 2001;0(05):-
Objective To explore the indications and clinical assessment for catherization in congenital heart diseased patients with stage operation. Methods Sixty five patients were selected from Apr. 1999 to Dec. 2002 undergoing second catherization. 47 male, 18 females with age ranging from 5 months to 22 years old on average of 6.8 years. EKG, chest X-ray, ECHO, cardiovascular pressure and blood oxygen saturation were taken in all patients during the procedure. Results According to the physiologic data recorded during the procedure of catherization, 39 cases needed one more surgery, 9 cases for intervention, 4 cases would be placed with endovascular stent 9 cases should be follow-up and 4 cases couldn t be operated again. Conclusions Catherization is not only offering the accurate physiologic data, but also revealing the details about anatomy with important information to the diagnosis and treatment for the congenital heart disease, especially for the srage-operation.
10.Evaluation of the MICROTEST 1 ESR analyzer and investigation of the reference value
Li-Ya LI ; Wei-Bin CHEN ; Feng GAO ; Shui-Fen SHEN ; Hui-Ping JIN ;
Chinese Journal of Laboratory Medicine 2001;0(03):-
0.37).Meanwhile a good correlation (Y=0.99X-0.18,r=0.987) was obtained. Though Westergren method correlated preferably with MICROTEST 1 (Y=0.86X+1.27,r=0.906),there was a markedly different (t=3.174,P=0.001).At last different references values were collected, according to sex and age.Male,32.5 mm/1 h(60 years old);Female, 34.03 mm/1 h(50 years old).Conclusions MICROTEST 1 correlated preferably with Westergren method.The examination by MICROTEST 1 needs small quantity of sample and fewer time.Furthermore,it has good repeatability and stability.The factors such as temperature and Hct have little influence on the results.The result suggested that it is suitable to apply MICROTEST 1 to large- scale clinical laboratory or other labs.But the reference value of ESR was influenced by age,which should be considered in clinical usage.