Objective To observe the influence of ascites in severe acute pancreatitis (SAP) on the function and viability of peritoneal macrophages in order to investigate the role of peritoneal macrophages in pathophysiological alteration and secondary pancreatic infection in SAP. Methods After the ascites of SAP models treated peritoneal macrophages for 1,3,6,12 and 24 hours in vitro, neutral red phagocytosis, cell viability and TNF secretion of peritoneal macrophages were determined respectively. Results The phagocytosis, cell viability and TNF secretion of macrophage all decreased with the treating time prolonged in the tests. Conclusions The ascites of SAP decreased the phagocytosis, viability and TNF secretion of peritoneal macrophages, and was one of the factors to promote secondary pancreatic and peripancreatic infection as well as bacterial translocation of gut.

Objective To study the antitumor effect of dendritic cell(DC) induced cytotoxic T lymphocyte (CTL). Method CTL induced by DC extracorporeally, were co-cultured with Bxpc-3 cells, CTL activity was observed by counting the killing of Bxpc-3 cells in vitro. Nude mice with Bxpc-3 cell transplant tumors were treated by injection of CTL on the edge of tumors, and kinetics of tumor growth was recorded, RT-PCR-ELISA was used to determine the telomerase of transplant tumor. Result CTL activity was 71.6%. Thirty-one days after transplantation tumor size and telomerase activity were not statistically different among therapy group and control group, whereas after fifty-five days tumor size (38?6)mm 2 , and telomerase activity (1.33?0.03) in CTL group were statistically different from that of ( 74? 33)mm 2 and (4.16?0.32) in control group. ConclusionDC induced CTLs suppress the experimental pancreatic tumor growth, providing an evidence for clinical immunotherapy of pancreatic cancer.

Objective To study the effect of preoperative selective arterial infusion chemotherapy (PSAIC) on large intestine cancer. Methods 63 patients with colorectal cancer underwent PSAIC with 5 fluorouracil,mitomycin and E adriamycin; the changes of clinical manifestation and pathology were observed and analyzed. Results (1)clinical manifestation:abdominalgia relieved in 18 patients and abdominal distention relieved in 16 patients.Improvement of hematochezia was found in 7 patients.In addition, of 13 patients with partial ileus, the clinical symptoms relieved in 9 patients. (2) pathology: there were karyopyknosis,karyorrhexis,coagulation and necrosis of cytoplasm in cancer cells. infiltration of inflammatory cells, edema and fibroelastosis in mesenchyne of cancer tissue. Proliferous intima and thrombus were also observed in the vessels. Most of these changes were moderate,and marked changes could also be seen in necrosis of cytoplasm and vessel intima proliferation. Conclusion PSAIC can control the focus of primary disease and the micrometastatic foci as well as improve the clinical symptoms, such as intestinal obstruction, and hematochezia, so PASIC is helpful to subsequent operation,and can improve the survival rate of patients with colorectal cancer.

Objective To observe the expression of connective tissue growth factor (CTGF) in pancreas, and discuss its significance. Methods The pancreatic fibrosis model was induced by high fat diets. The rats were sacrificed 16 weeks later, and the pancreatic tissue was harvested for routine pathologic examinations. Pancreatic collagen fibrosis I was determined by HE and Sirius red staining;α-SMA and CTGF expression were detected by immunohistochemistry. Results After pancreatic fibrosis, pancreatic lobules and acinar atrophy was observed, lobules gap was widened, interstitial fibrous tissue was significantly proliferated, the synthesis of pancreatic collagen fibrosis I was significantly increased when compared with normal pancreas ( 1500.2 + 255.8 vs. 57.4 ± 23.2, P ＜ 0. 01 ), the expression of α-SMA was significantly increased when compared with normal pancreas( 1500.2 + 255.8 vs. 57.4 + 23.2, P ＜ 0. 01 ), and the expression of CTGF was significantly increased when compared with normal pancreas (2950.5 ± 431.9 vs. 382.2 + 190.8, P ＜0.01 ), and there were abundant activated PSCs. Conclusions CTGF participated in the regulation of pancreatic fibrosis development; the function of CTGF was closely related to PSCs activation.

Purpose To investigate the expression of pFRK protein in non-small cell lung cancer (NSCLC) patients and to explore its prognostic value.Methods The expression of p-FRK protein in tumor tissues and corresponding adjacent normal pulmonary tissues from 162 NSCLC patients was detected by immunohistochemistry of EnVision two-step.The correlation among p-FRK expression,age,gender,clinicopathologic features,pTNM stage and metastasis of NSCLC patients was also analyzed.whether p-FRK could be used as an independent predictor of prognosis for patients with NSCLC was further determined.Results The positive expression rate of p-FRK in NSCLC tissues (51.9％) was significantly higher than that of adjacent normal lung tissues (11.7％) (P =0.001).The expression of p-FRK in NSCLC was significantly correlated with histopathologic type,lymph node status and pTNM stage (P ＜0.05).Kaplan-Meier survival analysis showed that there was a negative correlation between p-FRK positive rate and postoperative overall survival (x2 =17.849,P ＜0.001).Multivariate analysis showed that p-FRK expression was an independent prognostic factor for overall survival of NSCLC patients (HR =0.496,95％ CI:0.295-0.836,P ＜ 0.05).Conclusion As a novel NSCLC biomarker,the expression of p-FRK may predict a poor prognosis in the patients with NSCLC.

Objective To study the effect of Toll‐like receptor 4 inhibitor Fasudil on cerebral vasospasm after SAH .Methods A total of 60 New Zealand rabbits were randomly divided into three groups ,20 rabbits for each group ,SAH group:SAH model was established by autologous blood injection into the cisterna magna .Control group:saline was injected into cisterna magna .Fasudil group:Fasudil was injected into vein after SAH model .Vasospasm was valued by DSA and TCD .Seven days after operation basilar artery were collected .HE stain was used to observe vasospasm .TLR4 were observed by immunohistochemistry and western blot‐ting .Results Vasospasm model after SAH was successfully established .The basilar artery diameters were significantly shorter in the model group compared with the normal group (P<0 .01) .The artery diameter in Fasudil group increased significantly compared with SAH group(P<0 .01) .The expression of TLR4 decreased significantly in the Fasudil group compared with the model group (P<0 .05) .Conclusion Toll‐like receptor 4 pathway may be associated with cerebral vasospasm (DCV) .Fasudil could inhibit TLR‐4 expression and prevent cerebral vasospasm following subarachnoid hemorrhage .

Objective To study bacteria-blocking effect of surgical gowns with new material.Methods Semi-quantitative and qualitative testing methods were used to detect bacteria-blocking rates of key sites of surgical gowns(chest and forearm), the detected samples included sample A (composite material, unused), sample B (composite material, after washing 100 times), and sample C (monolayer material, unused).Results In semi-quantitative testing,the average bacteria-blocking rates of three samples were 75.47%, 70.78%, and 73.73% respectively.In qualitative testing,three samples could effectively block the penetration of Staphylococcus aureus under wet condition and Bacillus subtilis var.niger spores under dry condition.Conclusion In semi-quantitative testing, all three kinds of samples had bacteria-blocking effect, and the average bacteria-blocking rate was> 70%;in qualitative testing, three samples all meet requirements of bacteria-blocking effect under wet and dry condition.

ObjectiveTo explore the sensitivity of cancer stem cells to chemotherapy in human pancreatic carcinoma.MethodsPANC-1 ceils were cultured in an incubator filled with 5％ CO2 at a temperature of 37℃,and were labeled with Hoechst 33342.The SP analysis and sorting were performed using a FACSVantage SE.RT-PCR was performed to detect the expression of human CD133 ABCG2 and Notch1.SP and non-SP cells from the PANC-1 cell line were treated with 5-fluorouracil (5-FU; 1,10,or 100 μg/ml) or gemcitabine (10,100,or 1000μg/ml),and the cell viability was determined using the MTT assay.The sensitivity of sorted tumor cells to chemotherapeutics was determined in NOD/SCID mice model.ResultsSP cells were found to have higher drug-resistance both in vivo and in vitro and higher levels of mRNA expression for CD133,ABCG2 and Notch1,when compared to non-SP ceils.The xenografted tumors derived from injected SP cells and treated with gemcitabine had more CD133± cells than the untreated group.ConclusionsThe SP of PANC-1 pancreatic carcinoma cells are enriched with highly gemcitabine-resistant CSCs and determine the carcinogenesis of the PANC-1 pancreatic carcinoma cells.

ObjectiveTo assess and compare the resting energy expenditure measured by indirect calorimetry (MREE) and calculated with Harris-Benedict formula adjusted with correction factors (CREE) in critically ill surgical patients receiving mechanical ventilation,and to evaluate the relationship between resting energy expenditure and the severity of diseases.MethodsFrom August 2008 to February 2010,21 patients fitting the inclusion criteria were selected into the present study.The data of the patients were collected to calculate acute physiology and chronic health evaluation Ⅱ score ( APACHE Ⅱ score) and multiple organ dysfunction score ( Marshall score).MREEs were measured using indirect calorimetry of a MedGraphics CCM/D System,and CREEs were calculated at the same time with the Harris-Benedict formula.ResultsWithin the week of nutrition support,the mean CREE of the 21 patients was significantly higher than the mean MREE [ ( 8305.09 ± 1392.76 ) kJ vs.(6544.84 ±2079.65) kJ,P =0.000].The differences between MREE and CREE were statistically significant on the 0 ( P =0.000),1 ( P =0.000 ),2 ( P =0.000 ),and 4 day ( P =0.003 ) of nutritional support.There was no correlation between MREE and CREE (r =0.064,P =0.408 ),nor between MREE and APACHE Ⅱ ( r=-0.045,P =0.563 ).There was a correlation between MREE and Marshall score (P =0.001 ),but the correlation coefficient was low ( r =0.263).ConclusionsThe Harris-Benedict prediction modified with correction factors for severity of diseases overestimates the resting energy expenditure of critically ill surgical patients.Indirect calorimetry is a more accurate method for determining resting enenrgy expenditure.

event and treat circular,respiratory and renal insufficiency.