Adolescent ; Child ; Child, Preschool ; Diagnosis, Differential ; Female ; Follow-Up Studies ; Humans ; Infant ; Magnetic Resonance Imaging ; Male ; Retrospective Studies ; Spinal Cord ; pathology ; Spinal Cord Injuries ; diagnosis ; diagnostic imaging ; etiology ; pathology ; Spine ; diagnostic imaging ; Tomography, X-Ray Computed

Objective To investigate the expression of integrin linked kinase (ILK) in endothelial progenitor cells (EPC) of cord blood from patients with preeclampsia and its relation to placenta perfusion. Methods Thirty patients with preeclampsia,including 18 mild preeclampsia and 12 severe preeclampsia cases,and thirty-five normal late pregnant women were enrolled into this study.The expression of ILK mRNA and protein in EPC of cord blood was determined with RT-PCR and Western blot respectively.The angiogenesis ability of EPC was examined by tube formation in vitro.Comparisons of ILK mRNA and protein expression were done by analysis of variance and their relations to vascular tube formation were analyzed by correlation analysis. Results (1) ILK mRNA expression in normal group was higher than that in preeclampsia group (0.64± 0.05 vs 0.45 ± 0.06,q=18.76,P＜0.05) and the ILK protein expression in normal group was also higher (32 ±2 vs 26±1,q=18.47,P＜0.05).While the ILK mRNA and protein expression in mild preeclampsia group were also higher than that of severe preeclampsia group (ILK mRNA:0.47±0.07 vs 0.39±0.08,q=5.13,P＜0.05;ILK protein:25±2 vs 20±2,q=4.72,P＜0.05).(2) The tube formation capacity in preeclampsia group was significantly lower than that in normal group (135± 7 vs 330 ± 8,q=152.70,P＜0.05) ; the capacity was even much lower in severe group than in mild group (116±8vs 148±6,q=5.42,P＜0.05).(3) There was positive correlation between ILK mRNA and protein expression and tube formation capacity; the correlation index for normal group was 0.69 and 0.73(P＜0.05),0.67 and 0.72 in preeclampsia group (P＜0.05),0.65 and 0.68 in mild preeclampsia group (P＜0.05) and 0.63 and 0.74 in severe preeclampsia group (P＜0.05). Conclusions Decreased ILK level of EPC in cord blood might play an important role in the development of preeclampsia.

ObjectiveTo investigate the clinicopathological characteristics of non-diabetic renal diseases (NDRD) in the patients with diabetes mellitus.MethodsClinicopatholigical data of 202 patients with diabetes mellitus and NDRD identified by renal biopsy from January 1st,2003 to December 31st,2010 were analyzed retrospectively.All the patients were divided into three groups:the young (≤35 years old),the middle-aged (36-59 years old) and the elder (≥60 years old).Clinicopathological characteristics were compared among 3 groups.ResultsIn the young group (n=33),42.4％ of patients presented as chronic glomerulonephritic syndrome,while 36.4％ as IgA nephropathy for pathology.In the middle-aged group(n=136),35.3％ of patients presented as chronic glomerulonephritic syndrome,27.2％ as nephritic syndrome,17.6％ as chronic renal failure,14.7％ as latent glomerulonephritis,and 5.1％ as acute renal failure,while42.6％ as IgA nephropathy for pathology.In the elder group(n=33),30.3％ of patients presented as nephritic syndrome,30.3％ as chronic renal failure,while 27.3％ as membranous nephropathy for pathology.ConclusionsIn clinical manifestation,young patients are mainly chronic glomerulonephritic syndrome,middle-aged patients are diversified,and elder patients are mainly nephritic syndrome andchronicrenalfailure. Inpathology, youngandmiddle-agedpatientsaremainlyIgA nephropathy,and elder patients are mainly membranous nephropathy.

Objective To study the impact of active control of the trajectory of the center of gravity on the effectiveness of balance training and balance assessment. Methods Two groups of subjects ( group 1 30-45 years old, group 2 45-60 years old ) were evaluated and trained using both active center of gravity trajectory control and static balance methods. Results The percentages of success in controlling the center of gravity were the same when both groups were trained using static balance. Group 1's success percentage was higher than that of group 2 after active center of gravity trajectory training. Their affected lower limbs performed better in wave trace training than after static balance training, and performance improved with increased wave trace amplitude. Conclusions Wave tracing can stimulate the lower limbs of patients with active control force and improve their balance. The wave assessment is superior to static assessment, as it can objectively reflect ability in active center of gravity control and adjust the lower limbs of subjects whose static balance ability is at the same level.

Objective To explore the influence on the JNK signal transduction pathway by BaiHui-QuBin Scalp-acupuncture in rats of experimental acute intercerebral hemorrhage. Methods 192 healthy Wistar male rats were assigned into 3groups randomly:model group, model add pinprick group (to abbreviate:pinprick group), model add suppressor group(to abbreviate:suppressor group) ,containing 60 rats in each. All rats were induced be the intercerebral hemorrage model. Another 12 rats were assigned as the matched blank group. The pinprick group were acupunctured on the "Baihui" penetrating "Qubin". The influence surrounding the hamatoma of the proteinum expression of p-JNK of each group in deferent time phase point with the method of Western blot was observed. Results The results of expression of the proteinum p-JNK with the method of Western blot: all the rats after being made model appeared the phenomenon of the expression of p-JNK of rat's brain tissue surrounding the hematoma and increased to the peak in 2 days,but decreased gradually in 7days. In the same time phase point,compared with the model group,the expression of p-JNK of the pinprick group and the suppressor group were lower obviously (P ＜ 0.01). The pinprick group compared with the suppressor group, the expression of p-JNK showed no significant difference (P ＞ 0.05). Conclusion The therapy of BaiHui-QuBin Scalp-acupuncture could block the JNK signal tranaduction pathway, inhibit the expression of the proteinum p-JNK and so on have the function of protecting the nerve cell and inhibiting apoptosis.

Objective Expwre to the mechanism of the function of nerve protection to the secondary injury in the rat of intercerebral hemorrhage by acupuncture.Methods 160 healthy Wistar male rats were selected.The rats were assigned into 3 group randomly:model group,pinprick set,piracetam group.containing 50 rats in each.Each group were again divided into five time phase point of6 hours,1 day,2days,3days,7days.containing 10 rats in each.All rats were made to be the intercerebral hemorrhage model.Another 10 rats were assigned as the control.The pinprick group were acupunctured on the "Baihui" penetrating "Qubin".Results In the pinprick group,the expression 0fNGF of ratg brain dssue surrounding the hematoma was increased gradually and to the peak in 7days with the increasing of different time phase point.In the same time phase point,compare with the model group,the pinprick groupb expression of NGF was higher obviously.Both were relatively showed the significant difference(P＜0.01)and had statistical sigllificanle.Conclusion The therapy of acupuncture Can promote the expression of NGF of the rat'sbrain tissue surrounding the hematoma.

Acetates ; poisoning ; Acute Disease ; Humans ; Male ; Middle Aged ; Occupational Diseases ; Occupational Exposure ; adverse effects

In this paper, we analyse the general information of medical equipment clinical trials by clinical trial process management experience to elaborate the characteristics of the medical equipment clinical trials and the existent problems in our hospital in 10 years. We propose corresponding countermeasures to ensure the quality of medical tests, and improve the management of medical equipment clinical trials in hospital.
Clinical Trials as Topic ; Equipment and Supplies, Hospital ; Humans

Objective To understand the fluorescence pattern of antinuclear antibody(ANA)and the distribution of target anti-gen and ANA′s impact on rheumatoid arthritis(RA)RA.Methods The data of rheumatoid factor(RF)and anti-cyclic citrullinated peptide antibodies(anti-CCP antibodies)and ANA of 860 RA patients during 2014-2015 were collected and divided into 2 groups according to the ANA levels.The differences of RF and anti-CCP antibodies level between 2 groups were analyzed.Analyzed the fluorescence pattern of RA merger ANA and the distribution of target antigen by statistical.Results Of the 860 RA patients,the positive rate of ANA was 37.6%,the major fluorescence pattern of RA merger ANA was speckled pattern,anti SSA positive rate was 21.7%.The RF and anti-CCP antibodies level had statistical difference between ANA positive group and ANA negative group (P <0.05).Conclusion RA merger ANA positive can show different fluorescence patterns and target antigen,the speckled pattern and anti-SSA positive are the main pattern.The severity of arthritis symptoms and the degree of bone destruction between ANA positive group and ANA negative group were difference.Anti-ANA positive has a certain promoting effect on the progression of RA disease.

OBJECTIVE: To study the role of estrogen receptor (ER) and progesterone receptor (PR) in the formation of cholesterol calculus and investigate the effects of Shengqing Capsule (SQC), a Chinese patent herbal medicine with the function of soothing liver and draining gallbladder, on ER and PR expressions. METHODS: A total of 80 female guinea pigs were divided into normal control group, untreated group, ursodeoxycholic acid group (UDCA group) and SQC group. The cholesterol gallstone was induced by feeding the guinea pigs with high-fat lithogenic diet. SQC and UDCA were separately administered to the guinea pigs in the SQC group and UDCA group. After 7-week administration, all the animals were sacrificed to calculate the incidence of calculus formation and detect the expressions the ER and PR in the epithelial tissue of gallbladder by immunohistochemical method. RESULTS: Gallstone was cholesterol calculus detected by infrared spectrum. The incidence of calculus formation in the SQC group (27.78%) was significantly lower than that in the untreated group (81.25%) (X(2)=9.721 5, P=0.001 8). On the basis of Reiner standard, the expression distribution of ER and PR increased gradually from the normal control group through the SQC group and UDCA group to the untreated group. Except for the former two groups and the latter two groups, the differences between the other groups and UDCA group were statistically significant (P<0.05). Besides, the differences of positive expression rates between groups were statistically significant (P<0.05). CONCLUSION: Increased expressions of ER and PR are closely related to the formation of cholesterol stone. And Shengqing Capsule can down-regulate the expressions of ER and PR.