Objective To study the relationship between the expression of breast cancer susceptibility gene (BRCA)1 and the pathological parameters in sporadic breast cancer. Method Immunohistochemistry was used to detect the expression of BRCA1 in 42 cases of breast cancer tissues and 30 cases of breast fibroadenoma tissues and analyzed the correlation of the results with other parameters which included tumor size,tumor type,histological grade and axillary lymph nodes metastases. Results The positive expression rate of BRCA1 was 52.4% (22/42) in breast cancer patients and 85.7% (36/42) in breast fibroadenoma patients. The lower positive expression rate of BRCA1 was found in axillary lymph nodes metastases (P < 0.05). The positive expression rate in stage Ⅲ of breast cancer was lower than that in stage Ⅰ (P < 0.05 ) and was also lower than that in stage Ⅱ (P < 0.05 ), but there was no significant difference between stage Ⅰ and stage Ⅱ (P > 0.05). No significant correlation was observed among expression of BRCA1 and tumor size, tumor type (P > 0.05). Conclusions BRCA1 plays an important role in pathogenesis and development of breast cancer.BRCA1 maybe a adjuvant index for treating breast cancer and estimating prognosis.

This paper analyzes the necessity of offering medical English courses at the preclinical stage,introduces the online medical English listening course,summarizes its characteristics:rich in content,highly interactive,quick in feedback,fair in assessment,and puts forward some suggestions for perfecting this course in the future.

Objective To compare the operations for patients with colorectal cancer through laparoscopic or traditional surgery.Methods Sixty-one colorectal cancer patients,who underwent laparoscopic surgery were enrolled into the observation group.Forty patients with colorectal cancer,who underwent traditional surgery were enrolled into the control group.The operation time,intraoperative blood loss and hospitalization time were observed.Results The operation time of the control group was less than that of the observation group(P ＜ 0.01).The intraoperative blood loss and hospitalization time of the observation group were less than those of the control group (P ＜ 0.01).In the lymph node dissection,there was no significant difference between two groups (P ＞ 0.05).Conclusion Laparoscopic surgery for treatment colorectal cancer can effectively remove cancer cells,dissect lymph nodes.

Cell Movement ; drug effects ; Chemokine CXCL12 ; genetics ; metabolism ; pharmacology ; physiology ; Humans ; Neoplasm Invasiveness ; physiopathology ; Neoplasm Metastasis ; physiopathology ; Neoplasms ; metabolism ; Neovascularization, Pathologic ; physiopathology

Antigens, Neoplasm ; genetics ; metabolism ; Breast Neoplasms ; enzymology ; genetics ; metabolism ; DNA Topoisomerases, Type II ; genetics ; metabolism ; DNA-Binding Proteins ; genetics ; metabolism ; Female ; Gene Expression Regulation, Neoplastic ; physiology ; Humans

Objective: To observe the inhibitory effect of triplex forming oligonucleotede (TFO) of PDGF-B chain combined with antisense oligonucleotide (AON) of VEGF on glioma growth in rats. Methods: Thirty -six male SD rats received 1×106 C6 glioma cells/20 μl normal saline into the right caudate putamen by stereotactic technique. Group I (n=6) was treated in situ with 1.5 mg/20 μl TFO for 3 times on 8,11 and 14 d after glioma cell inoculation. Group II (n=12) and III (n=12) were respectively treated with TFO 1.5 mg+AON 0.125 mg/20 μl and TFO 1.5 mg+AON 0.250 mg/20 μl for 3 times on 8,11 and 14 d after glioma cell inoculation. Control group (n=6) was treated with 20 μl saline for 3 times at the same time pionts. Three weeks after cell inoculation, all rats were sacrificed to observe tumor growth and to determine the expression of PDGF-B,VEGF and PCNA. Results: The inhibiti on rate of tumor growth was 53.1% in group I, 81.4% in group II and 93.1% in group III, with significant difference found between the 3 groups (P<0.01). PDGF-TFO had obvious inhibitory effect on the expression of PDGF-B, VEGF and PCNA in C6 glioma cells;and PDGF-TFO combined with VEGF-AON had more potent inhibitory effect on the expression of PDGF-B, VEGF and PCNA in C6 glioma cells. Conclusion: PD GF-TFO combined with VEGF-AON has more potent inhibitory effect on tumor growth than PDGF-TFO alone.

Objective:To evaluate the value of adjuvant radiotherapy for stage Ⅱ endometrial cancer after surgery.Methods:40 patients with stage Ⅱ endometrial cancer with over five years follow up were analyzed vetrosectively.27 cases received subradical hysterectomy,and additional pelvic lymph node dissection in 13 cases.6 cases were treated with surgery alone.34 cases were treated with postoperative pelvic irradiation and additional vaginal brachytherapy in 12 cases.Results:The 5 year survival rate,pelvic failure rate and extrapelvic recurrence rate were 65%(26/40),17.5%(7/40),25%(10/40) repectively.The 5 year disease free survival rate,pelvic failure rate and extrapelvic recurrence rate were 70.5%(24/34),5.9%(2/34),29%(10/34) respectively in 34 patients with postoperative radiotherapy.5 cases with surgery alone recurrenced at the vaginal residual within two years.The late complications were 44%(15/34),including proctitis,cystitis and intestinal obstruction.Conclusion:Postoperative radiotherapy for stage Ⅱ endometrial cancer can improve the tumor local control and increase the late complications.

Alveolar Process ; physiology ; Animals ; Biocompatible Materials ; therapeutic use ; Bone Matrix ; transplantation ; Bone Regeneration ; physiology ; Bone Transplantation ; methods ; Dental Implantation ; methods ; Esthetics, Dental ; Guided Tissue Regeneration, Periodontal ; methods ; Humans ; Prosthodontics ; methods ; Tooth Extraction ; methods

Objective To explore the feasibility of applying MRI cranial surface locating device in localization of small intracranial space-occupying lesions before the surgeries. Methods Thirty patients with small intracranial space-occupying lesion were localized by MRI cranial surface locating device before the surgeries. The clinical accuracy was verified during the surgeries and the results were compared with cases without using the device. Results According to the verifications during the surgeries , 30 cases which used MRI cranial surface locating device showed much higher accuracy than that using the traditional method. Conclusions MRI cranial surface locating device can precisely delineate the surgical target area. The device is easy to operate and also can help to design the rational surgical approach with minimum injuries , significantly improve accuracy of locating small intracranial space-occupying lesions and it is safe , effective and worthy of widely used clinically.

Objective To determine and compare the minimum local analgesic concentration(MLAC) of ropivacaine and bupivacaine in continuous block of axillary brachial plexus for postoperative active mobilization of flexor tendon. Methods Seventy ASA Ⅰ or Ⅱ patients undergoing relaxation of flexor tendon were randomly allocated into 2 groups (n = 35 each): ropivacaine group (group R) and bupivacaine group (group B). A catheter was inserted into axillary sheath using B. Braun stimulating intraducer, 24-48 h after operation. 30 min before functional exercise 20 ml of ropivacaine or bupivacaine was injected through catheter. The minimum local analgesic concentration of both local anesthetics was determined by up-and-down sequential test. The first concentration of both local anesthetics was 0.1%. The next concentration of local anesthetic was determined by response of the previous patient. The analgesia efficacy was assessed using 100 mm VAS score (0 = no pain, 100 mm = worst possible pain) . Increase/decrease in VAS by 10 mm was defined as effective. An effective result resulted in increase/decrease by 0.01% in the next patient. The motor block was also recorded.Results The two groups were comparable with regard to age, sex, body weight and baseline VAS score. The MLAC of ropivacaine was 0.133% [95% confidence interval (CI), 0.128%-0.138%] and MLAC of bupivacaine was0.121% (95%CI, 0.116%-0.126%). The analgesic potency of ropivacaine was 10% less than that of bupivacaine. The potency ratio of ropivacaine to bupivacaine was 0.91. The degree of motor block is higher with bupivacaine than with ropivacaine. Conclusion The results of our study show that the analgesic potency of ropivacaine is 10% less than that of bupivacaine. The degree of motor block is higher with bupivacaine than with ropivacaine.