With the development of conformal radiotherapy,radiotherapy has been used not only in localized prostate cancer as curative therapy.In the treatment of locally advanced prostate cancer,adjuvant therapy after radical prostatectomy and combination therapy with androgen can be used,and can relieve the patients with advanced prostate cancer.The indication,efficiency and side effects of the different techniques are reviewed as follows.

Objective To generate specific targeting T cells against gastrointestinal cancer, a eukaryotic expression vector encoding carcinoembryonic antigen(CEA) specific single chain variable fragment(scFv) fused to the transmembrane and intracellular domains of the signal transducing chain of CD3? was constructed. Methods Anti CEA scFv cDNA was obtained by RT PCR. The transmembrane and intracellular domains of CD3?cDNA was amplified from human T lymphocyte using RT PCR to clone into a eukaryotic expression vector, and anti CEA scFv cDNA was inserted upstream of CD3?. Finally, the eukaryotic expression vector containing scFv CD3? pcDNA3.0 was constructed and sequenced. Results A 810 base pair of anti CEA scFv was in accordance with sequence concerned, a 438 base pair of cDNA of the transmembrane and intracellular domains of CD3? was confirmed as sequence concerning of GenBank. Further identification was carried out through agarose electrophoresis after enzyme digestion. Conclusion We constructed a fusion molecular with both CEA targeting and CD3? signal transduction perporties, which lay a good foundation for generation of modified cytotoxic T lymphocytes against gastrointestinal tumors.

Purpose:Analysing the imaging performance of solitary plasmacytoma of spine,to raise the awareness and the diagnosis accuracy.Materials and Methods: Five cases of spinal solitary plasmacytoma confirmed by clinical pathology were retrospectively analysed,to sum up the imaging performance characteristics.Results: All lesions were single vertebra involved,3 cases in thoracic and 2 cases in lumbar,3 cases occurred vertebral compression fracture.X-ray and CT showed osteolytic destruction,expansive growth and soft tissue mass,MRI showed diversity of signals,and remarkable enhancement after contrast injection.Conclusions: X-ray presents lower diagnostic sensitivity,but contributes to exclude multiple myeloma.CT and MRI can depict the morphological features and extent of tumor regions definitely,CT shows better than MRI on the thickening trabecula and residual bone in the tumor,MRI can provide a lot of information for plasmacytoma,including the diagnosis,identification and staging,which is considered as the best imaging diagnostic methods of plasmacytoma.

Objective To construct and identify the Eukaryotic expression vector of RNAi hTRET. Methods The hRERT interference sequence was synthesized by artificial way and was directed cloning to the Eukaryotic expression vector pSilencer 3.1 H1 neo,and was identified by pcr,enzyme cutting,sequencing,determining its concentration and purity. Results We construct the Eukaryotic expression vector of RNAi hTRET successfully. Conclusion Construction of Eukaryotic expression vector of RNAi hTRET established foundation for biological effectiveness of telomere and telomerase in cancer cells.

Objective To construct the double hairpin siRNA and green fluorescent protein(GFP) expressing vector pU6/double-siRNA/Neo/GFP/1A/2A to interfere 1A and 2A gene of Coxsackie virus B4.Methods 21 bp fragments of the Coxsackie virus B4 2A and 1A gene were chosen as the targets and 65 bp complimentary fragments were synthesized,then the target gene fragments were cloned into pSilencer2.1U6 Neo and pGCsi-U6/Neo/GFP/siNeGative,respectively,then the double siRNA expressing vector pU6/double-siRNA/Neo/GFP/1A /2A was constructed by restrict endonuclease digestion,elctrophoresis isolation and reclaimer,ligatied by T4 DNA ligase;then the double siRNA expressing plasmid was transfected into Hela cells,and the GFP was observed under fluorescent microscope.Results The correct results showed that the recombinant plasmid had the correct special fragments and DNA sequence detected by restrict endonuclease digestion, electrophoresis and DNA sequencing;and GFP was also observed in Hela cells tansfected with pU6/double-siRNA/Neo/GFP/1A /2A under fluorescent microscope more than 15 d after transfection.Conclusion The double siRNA expressing vector pU6/double-siRNA/Neo/GFP/1A/2A is constructed successfully;it has the correct target viral gene sequences and can express GFP gene in Hela cells more than 15 d after transfection.

Objective To analyze the relationship between serum intermedin ( IMD ) , CystatinC ( CysC ) and fibro-blast growth factor23(FGF23) with hypertensive left ventricular hypertrophy (HLVH). Methods Serum IMD,Cy-sC and FGF23 levels of 30 patients with essential hypertension(EH group),30 hypertensions with LVH(HLVH group)and 30 healthy subjects(control group)were detected by enzyme-linked immunosorbent assay(ELISA). All the subjects did UCG for LVMI. Results LVMI and serum IMD,CysC,FGF23 levels were significantly higher in EH group than in control(P<0. 05),higher in HLVH group than in EH and control groups(P<0. 05). In the pa-tients with hypertension LVMI and serum IMD,CysC,FGF23 levels were increasing with increased blood pressure levels. LVMI level was positively correlated with serum IMD,CysC and FGF23 levels(r=0. 769,0. 517,0. 700;P<0. 01). Line to LVMI level as the dependent variable,multiple stepwise regression analysis showed that:systolic blood pressure( SBP) ,IMD,CysC,homocysteine( Hcy) ,FGF23 entered regression equation. They were independent risk factors for LVMI. Conclusion Serum IMD, CysC and FGF23 levels are closely associated with EH and HLVH. They may participate in the development of EH and affect the process of HLVH. Combined-detection of ser-um IMD,CysC and FGF23 can be used as a reference index for the condition and prognosis of HLVH.

Experiment teaching is an important component of teaching in medical college and university, a key step to foster students practical ability and innovation ability and a guarantee to achieve the goal of medical talent training. The quality and level of experiment teaching is directly related to the college talent training quality and the college overall development. According to the requirement of quality engineering, our college strengthens the connotation construction from the aspects of experiment teaching management, teaching staff training and quality monitoring and improves experiment teaching quality.

Objective To investigate the influence of military exercise on some biochemical indexes in soldiers.Methods The level of serum soluble tumor necrosis factor receptor-1(sTNFR-1)and soluble intercellular adhesion molecule-1(sICAM-1)were determined with ELISA in peripheral blood of 133 servicemen who participated in the excercise before and after the military exercise.At the same time,the levels of serum gastrin and tetosterone were determined by radioimmunassay(RIA),the level of superoxide dismutase(SOD)and malonyl dialdehyde(MDA)were determined by biochemical methods.The correlation between the changes in these contents and psychological stress was studied.Results The levels of serum sTNFR-1,sICAM-1,testosterone and MDA of 133 servicemen increased after the military exercise compared with that of before the exercise(P0.05).Conclusion As a strong stress,military exercise can inhibit the immunological function and produce changes in emotional state,gastroenterological hormones,sex hormones etc.It is necessary to carry out mental and physiological training,so that the ability of servicemen to withstand the military stress can be improved.

Objective To explore the surface electromyography(EMG)characteristics of biceps brachii during isometric contraction in children with hemiplegic cerebral palsy.Methods The surface electromyograms signals of biceps brachii of 10 children with hemiplegic cerebral palsy during passive isometric contraction were recorded.Results The intact side registered a stronger EMG than that of the affected side,the integrated electromyography(iEMG)and root mean square(RMS)levels from the intact side were higher than those of affected side significantly(P<0.05).Conclusion The results of electromyographic analysis are consistent with Modified Ashworth Scale.Electromyographic analysis can provide a satisfactory evaluation of the muscle tone in children with hemiplegic cerebral palsy.

Objective To observe the effect of static balance test and training equipment on the equilibrium function in children with spastic diplegia.Methods 24 children with spastic diplegia were treated with visual feedback balance training besides routine rehabilitation training.All cases were assessed with Berg Balance Scale(BBS) and the static balance test and training equipment when eyes opened and closed,before and after the 8-week training.Results After the 8 week-training,the BBS scores of all children were higher than that before training(P<0.05).In the segment of the static balance test,fall index,weight distribution index and stability index decreased significantly(P<0.05～0.01).Conclusion The equilibrium function of the children with cerebral palsy can be assessed and pertinent training can be performed with balance equipment.