Objective To investigate the application validity of the adjusted norm of WAIS-RC in the identification of intellectual disability.Methods128 patients with mental retardation (MR) were chosen for identification of the intellectual disability, measured their full scale IQs (FIQ) according to the original norm, calculated their four-subtest short forms FIQ respectively by Tellegen way according to the original standard norm and the new adjusted norm, then compared and analyzed the outcomes.ResultsThe short forms FIQ according to the original and the new norm had high correlation to full forms FIQ (P<0.01). The average of the short forms FIQ was higher than full forms FIQ according to the original norm (P<0.01), showing no significant difference according to the new norm(P＞0.05). In severe intelligence defected group according to full forms IQ, the grade classification corresponding rate of short forms FIQ according to the new norm was 0.00%, as well as the original norm. That in medium and mild intelligence defected groups was higher than that of original norm(P＜0.01).ConclusionThe test validity of adjusted norm short forms of WAIS-RC is superior to the original norm, but not suitable for severe intelligence defected.

Objective To study a rehabilitation nursing menus for neurogenic large intestine dysfunction.Methods Various nursing approaches were used for defecation dysfunction.Results and Conclusion 94.60% patients improved in the second week,which including gained more awareness,control,and spend less time of defecation.

Age Factors ; Endometrial Hyperplasia ; pathology ; Endometrial Neoplasms ; pathology ; Endometrium ; cytology ; pathology ; Female ; Humans ; Menstrual Cycle ; Papanicolaou Test ; Postmenopause ; Vaginal Smears

Adult ; Coloring Agents ; adverse effects ; Diagnosis, Differential ; Humans ; Lymphadenitis ; chemically induced ; Male ; Melanoma ; secondary ; Tattooing ; adverse effects

The recent progress in neural stem cells (NSCs) research has shed lights on possibility of repair and restoration of neuronal function in neurodegenerative diseases using stem cells. Induction of stem cells differentiate into mature neurons is critical to achieve the clinical applications of NSCs. At present, molecular mechanisms modulating NSC differentiation are not fully understood. Differentiation of stem cells into neuronal and glial cells involves an array of changes in expression of transcription factors. Transcription factors then trigger the expression of a variety of central nervous system (CNS) genes that lead NSCs to differentiate towards different cell types. In this paper, we summarized the recent findings on the gene regulation of NSCs differentiation into neuronal cells.

Carcinoma, Squamous Cell ; diagnosis ; Carcinoma, Verrucous ; diagnosis ; Epidermis ; pathology ; Humans

Humans ; Occupational Exposure ; Occupational Health ; Risk Assessment ; Workplace

Objective To compare the clinical efficacy and treatment duration between two methods,tuina plus acupuncture at Qiaogong point predominantly versus conventional tuina method,in treating children with muscular torticollis.Method A hundred children with muscular torticollis were randomized into a treatment group and a control group,50 cases each.The treatment group was intervened by tuina plus acupuncture at Qiaogong point predominantly,while the control group was intervened by conventional tuina method.The clinical efficacies and treatment durations were compared between the two groups.Result The total effective rate and recovery rate were respectively 96.0％ and 80.0％ in the treatment group,versus 90.0％ and 42.0％ in the control group.There was a significant difference in comparing the recovery rate between the two groups (P＜0.05).The between-group difference in comparing the time taken for the effective and recovered cases was statistically significant (P＜0.05).Conclusion Tuina plus acupuncture predominantly at Qiaogong point can produce a more significant efficacy and it takes a shorter time in treating children with muscular torticollis compared to the conventional tuina method.

OBJECTIVETo develop a nestin transgenic mice and study the distribution of nestin expression in the organs.

METHODSThe three segments of nestin in full-length cDNA was amplified using human glioma cell line U251 cDNA as the template and cloned into the vector pBROAD3 containing a strong promoter ROSA26. The constructed vector, after identification with restriction enzyme and sequencing and removal of the prokaryotic sequences, were purified and injected into the fertilized eggs of mice. Transgenic mice were identified by PCR and the founder was maintained. Western blot analysis was used to detect the expression of nestin of the F3 transgenic mice and the wild-type ones in the vital organs (heart, lung, brain and kidney).

RESULTSThe Nestin transgenic vector controlled by ROSA26 promoter was successfully constructed and validated by sequencing. Among the 34 newborn mice, 2 founders were tested to be nestin-positive by PCR. Westem blot analysis showed that the F3 transgenic mice expressed high levels of nestin in the brain and lungs.

CONCLUSIONNestin transgenic mice have been successfully established with stable nestin expression in the brain and lungs of the offspring mice, which can be useful for studying the functions of nestin in tumor metastasis, stemness maintenance and differentiation of cells.

Animals ; Cell Line ; Genetic Vectors ; Humans ; Mice ; Mice, Transgenic ; Nestin ; genetics ; Promoter Regions, Genetic ; RNA, Untranslated ; genetics

Objective Radionuclide-labeled low molecular weight polypeptide is reeently advocated for the diagnosis and treatment of malignant tumor. The purpose of this study was to evaluate the anti-tumor effect of 131Ⅰ-Tyr-octreotide in nude mice bearing human non-small cell lung cancer (NSCLC). Methods 131Ⅰ-Tyr-octreotide was prepared by Ch-T method. The radiochemical purity was measured and biodistribution was evaluated. The nude mice models bearing human NSCLC were studied and divided into four groups: group A injected 131Ⅰ-Tyr-octreotide through tail vein, group B injected normal saline, group C injected 131Ⅰ-Tyroctreotide through stroma and group D injected 131Ⅰ through stroma. The radioactivity ratio of tumor to normal tissue (T/NT) was calculated over region of interest (ROI). The tumor cell cycle and cell apoptosis were analyzed by flow cytometry (FCM), terminal deoxynucleotidyl transferase mediated dUTP-biotion nick end labeling (TUNEL) and histopathological analysis. Statistical analysis was performed with SPSS 11.0, and the comparison for difference between groups performed with one-way ANOVA analysis. Results The labeled radiochemical purity was (95.23±1.67)% and specific activity of 3.5×106Bq/ug. The biodistributiou showed high uptake in kidney, and low uptake in liver and spleen. The radioactive uptake in group C was higher than the other groups, and the retention time was longer. The T/NT was 52.74±0.13 after 24 h, which was much higher than that the other groups (group D: 8.90±0.23, group A: 6.42±0.02, q=628.81 and 664.33, all P<0.05). The resuits of tmnor cell cycle determined by FCM showed that the G1 phase was blocked mast remarkably in group C than the other groups [group C: (83.17±6.86)%, group A: (57.02±18.81)%, group D: (49.29±7.80)%, group B: (45.88±5.13)%, q=5.29, 6.86, 7.55, 1.56, 2.26, 0.69, all P<0.05]. Apeptotic cells were observed by TUNEL, and apoptotic body was detected by immuno-histochemical examination. Conclusions 131Ⅰ-Tyr-octreotide was easily labeled by Ch-T. 131Ⅰ-Tyr-octreotide could induce tumor cell apoptosis and inhibit the tumor cell of NSCLC. It might be a potential target-directed agent in NSCLC.