Objective:This study aimed to analyze the prognostic factor of bilateral hydronephrosis caused by advanced cervical cancer and evaluate its value of treatment. Methods:A total of 40 patients with bilateral ureteral obstruction secondary to cervical cancer were diagnosed through computerized tomography, radioactive nephrogram, and blood tests for renal function. The placement of retrograde internal double-J ureteral stents was performed under a cystoscope in 13 patients. The placement of antegrade internal double-J ureteral stents via percutaneous nephrostomy was performed in 25 patients. Two cases had external ureteral stents via percutaneous nephrostomy. Twenty-nine patients underwent radiotherapy after normalization of their blood urine nitrogen and creatinine levels. The prognostic value of the treatment and renal function before placement of ureteral stents and radiotherapy after placement of ureteral stents were analyzed. Results:The normalization rate of renal function after ureteral stenting was 91.3%(21/23). The median survival time was longer in patients with untreated cervical cancer than that in patients with recurrent cervical cancer (χ2=9.379, P=0.009). After ureteral stenting, the median survival time was longer in patients who underwent radiation therapy than that in patients untreated with radiation (χ2=17.329, P=0.000). The median survival time was not significantly influenced by renal function before placement of ureteral stents (χ2=1.37, P=0.242). Conclusion:The patient with bilateral ureteral obstruction from untreated cervical cancer or from recurrent pelvic disease after surgical therapy should be considered for ureteral stenting followed by appropriate radiation.

Objective To study bevacizumab combined with chemotherapy regimen in treatment of different metastasis survival in patients with colorectal cancer, in order to optimize the scheme selection of bevacizumab for metastatic colorectal cancer metastasis , improve survival and quality of life of patients with rectal cancer.Methods 128 cases of late January 2013~2013 year in June in general hospital of medical university for treatment of metastatic colorectal cancer patients as the research object, which were randomLy divided into A, B, C, D 4 groups, 32 cases in each group, respectively with bevacizumab (5 mg/kg) combined with FOLFIRI and bevacizumab (10 mg/kg) combined with FOLFIRI and bevacizumab (5 mg/kg) combined with FOLFOX and bevacizumab ( 10 mg/kg ) therapy combined with FOLFIRI regimen, four patients underwent 6 cycles of treatment.Comparison of four groups of patients with recent clinical efficacy, progression free survival (survival progression-free, PFS), three years survival rate, overall survival (overall survival, OS).Results Four groups of patients with gender, age, stage of disease, ECOG score before treatment, primary tumor site, tumor metastasis, metastasis tumor diameter, there were no significant differences.Toxicity of different degrees occurred in the four groups during treatment, but the patients tolerated and complete the established chemotherapy.A, B, C, D group total effective rate were 56.25%, 59.38%, 46.88%, 50%, 3 years survival rates were 25%, 18.75%, 28.13%, 18.75%, PFS respectively: (9.5 ±1.1), (9.6 ±1.1), (9.2 ±1), (9.3 ± 0.9) months, overall survival were: (30.23 ±2.26), (29.83 ±2.24), (26.94 ±2.08), (27.19 ±2.11) months.A, B group of patients in the clinical total effective rate, three years survival rate were higher than that of C and D two groups, but there were no statistically significant difference between the two groups.A, DFS and TTP in B group were significantly higher than that of C and D two groups, and there were significant differences among the groups (P all <0.05), the difference between the two groups was no statistically significant, C, D between the two groups there was no significant difference, A, B groups were significantly higher than that of C, OS in D two group, and the difference between the two groups were statistically the significance (P<0.05), A, B between the two groups, but no significant difference, C, D between the two groups there was no significant difference.Conclusion according to the 5-10 mg/kg/m2 of bevacizumab combined with FOLFIRI regimen in the treatment of metastatic colorectal cancer, can effectively improve progression free survival in patients with a high clinical value to promote the transfer of clinical overall survival of patients with colorectal cancer.

Increased evidences indicate that resistin is a new hormone secreted from adipose tissue, it is reported to be an important signal molecule linking between obesity,insulin resistance and type 2 diabetes. Many factors can affect the gene expression of resistin. However, the detailed function of resistin still remains mysterious and much work needs to be undertaken. [

Objective: To examine the development of digit, verbal and spatial working memory span in adults, and explore the factors which can affect them.Methods:The subjects were 280 healthy adults (male 134, female 146) aged from 18 to 49 years old.Each subject was administrated three subtests of the Multiple Memory Assessment Scale (MMAS), including digit, verbal and spatial working memory tasks, and demographical data were collected.Results:The adult demonstrated a significant longitudinal decline in performance of tests of working memory span (F=2.96~6.87,P

Objective To investigate the value of diffusion tensor tractography (DTT) quantitative assessment of corticospinal tract (CST) damage in acute intracerebral hemorrhage (ICH). Methods Thirty-two patients were enrolled in this study. DTT was obtained in early stage of ICH. The CST was reconstructed with Volume One 1.64 and diffusion Tensor Visualizer (dTV) software from Tokyo University. The compression, the displacement, the destruction, and the relative remaining number of the CST were calculated. Results The compression, the displacement and the destruction of the CST were observed in DTT images in all patients. The correlation coefficient between remaining number of the CST and score of Fugl-Meyer after the onset of ICH was 0.75 (P＜0.01). Conclusion DTT can quantitatively evaluate the state of CST injury in acute ICH.

Objective To study the effect of salmon calcitonin and physical therapy on lumbar spinal ste- nosis.Methods Eighty cases of lumbar spinal stenosis were divided into treatment and control groups.Physical therapy alone was given to the control group,but salmon calcitonin was injected intramuscularly in addition to phys- ical therapy for the patients in the treatment group.Visual analogue scale,range of motion (ROM),pain-free walking distance,tendon reflexes and functional independence measures (FIM) were observed to assess the re- sults.Results The VAS,ROM and walking distance of the treatment group improved more than those of the con- trol group,but the groups' tendon reflexes and FIM were similar.Conclusions Salmon caleitonin can reduce the symptoms of lumbar spinal stenosis,and it has special effects in relieving pain.

OBJECTIVETo evaluate the effects of Attractin (Atrn) silence on the anti-oxidative and anti-apoptotic abilities of TM4 Sertoli cells and its influence on the expressions of superoxide dismutase (SOD) and caspase6 in the cells.

METHODSWe observed the apoptotic indexes of TM4 Sertoli cells with normal expression (control), partial deletion, and complete deletion of the Atrn gene (psiRNA-TM4, psiAtrn-TM4, and mu-SC). We determined the mRNA and protein expressions of SOD and caspase6 by Q-PCR and Western blot, measured the SOD activity and malondialdehyde (MDA) contentby spectrophotometry, and detected the apoptotic index of the cells by TUNEL.

RESULTSCompared with psiRNA-TM4, after inhibition of the Atrn expression, the Sertoli cells in the psiAtrn-TM4 and mu-SCgroups showed significantly decreased expressions ofSOD mRNA (70.76% and 92.58%) and protein (65.11% and 71.0%) (both P < 0.05). The levels of caspase 6 mRNA and protein were increased 5.28 and 3.40 times in the psiAtrn-TM4 and 2.97 and 2.50 times in the mu-SCgroup as compared with the normal control (both P < 0.05). Atrn deletion markedly increased the apoptotic indexes of the cells in the psiAtrn-TM4 and mu-SC groups by 16.22% and 22.03% (P < 0.05) and reduced the activity of SOD by 23.00% and 39.37% (P < 0.05); it also elevated the level of MDA by 155.22% (P < 0.05).

CONCLUSIONThe Atrn gene exerts influence on the function of Sertoli cells in multiple ways, in which antioxidative stress and apoptosis regulation may play an important role.

Animals ; Apoptosis ; Caspase 6 ; metabolism ; Cells, Cultured ; Gene Deletion ; Male ; Membrane Proteins ; genetics ; metabolism ; Mice ; Oxidative Stress ; Sertoli Cells ; metabolism ; pathology ; Superoxide Dismutase ; metabolism

Objective To explore the outcome of the tibial bone-skin flap grafts in the management of severe traumatic osteomyelitis complicated with bone and skin defect in leg.Methods Twenty-seven cases of the traumatic osteomyelitis complicated with bone and skin defect in leg were treated with vascularized tibial bone-skin flap grafe from August, 2007 to November, 2013.Reconstruction of limb tibia continuity and cover the wound.Results The tibial bone-skin flaps were completely survived in 26 of the 27 cases except 1 ease which was repaired by adjacent flap because of the disorder blood circulation.The followed-up showed that all flaps had good blood circulation.The infection was controlled completely.The leg function and contour were satisfactory.Conclusion The tibial boneskin flap has the advantages of abundant blood supply, full bone-skin flap supply, shortens hospitalization and suitable for treatment of traumatic osteomyelitis complicated with bone and skin defect in leg.

ObjectiveTo observe the effect of early systemic rehabilitation trainings on the function recovery in patients after replantation of severed digit. Methods21 patients were planned systemic rehabilitation trainings more than 2 months after operation. Total Active Measurement (TAM) and Two Points Distance (2-PD) were used for the assessment of motor and sensory function. Employment situation were also followed-up after being discharged from hospital. ResultsThe omni-efficiency of motor and sensory function of the 21 cases was 92.3% and 88.5%. 19 cases of them returned to the original work, 2 cases changed another work. ConclusionThe early systemic rehabilitation trainings are very useful for the hands function and vacation ability of the patients after replantation of severed digit.

Objective To observe the effect of pretreatment of aprotinin on nitric oxide (NO) and nitric oxide synthase (NOS) contents after ischemia-reperfusion injury of spinal cord in rabbits.Methods 45 rabbits were randomly divided into aprotinin treatment group (group A), normal saline control group (group B) and pseudo-surgical operation group (group C) with 15 rabbits in each group. The infrarenal segment in abdominal aorta was clamped for 60 min to construct the model of lumbosacral spinal cord ischemia in rabbits. Reperfusion was followed and kept on for 24 h until the blood flow regained normal. Aprotinin was given 3×107 IU/kg as a short time intravenous injection for 10 min before ischemia, and then was drilled with micro pump by 1×107 IU/kg/h. Normal saline was used in group B, the ischemia-reperfusion duration between group A and group B remained same. The group C was only exposured abdominal aorta and not clamped. The rabbits were killed before ischemia and at 8 h, 24 h after ischemia-reperfusion, lumbar segment spinal cords were harvested to detect contents of NO and NOS of spinal cord.Results After 8 h of ischemia-reperfusion,the contents of NO, total NOS (TNOS), and induced NOS (iNOS) in group A and group B were more than that before ischemia (P＜0.05). After 8 h of ischemia-reperfusion, there was a significant difference in the contents of NO, TNOS, iNOS between group A and group B (P＜0.05～0.01). After 24 h of ischemia-reperfusion, there was a significant difference too between group A and group B (P＜0.01). After 8 h and 24 h ischemia-reperfusion, the contents of NO, TNOS, iNOS in group A and group B were more than that in group C (P＜0.01).Conclusion During the ischemia-reperfusion, more NO produced is an important factor of spinal cord injury. Aprotinin can decrease the contents of NO and ischemia-reperfusion injury to spinal cord of rabbits.