2.Determination of ChrysophanoL in Kuiyanqing Xiangkouye by HPLC
Junxuan YANG ; Li CHEN ; Xiaodong PU
Traditional Chinese Drug Research & Clinical Pharmacology 2000;0(05):-
Objective: To establish a determination method of chrysophanol in Kuiyanqing Xiangkouye . Methods:HPLC with DiamondTM C18 column(4.6?200mm,5?m)was used . Methanol: 0.1 %phosphoric acid(85:15)was used as mobile phase . The flow rate was 1.0mL/min . The detection wavelength was at 254nm . Results:The linearity of chrysophanol was in the range of 0.06462~1.03392 ?g . The recovery was 97.67 %and RSD was 1.37 %. Conclusion:This method is convenient and with a good resolution and can be used for the quality control of Kuiyanqing Xiangkouye .
3.Relationship of chronic myelocytic leukemia and angiogenesis factors
Feng LI ; Pu CHEN ; Shanhua ZOU
China Oncology 2001;0(05):-
Purpose:To study the secretion and gene expression of Angiogenesis factors in the patients with CML and study the effect of Angiogenesis in the occurrence and development of CML. Methods:Concentration of VEGF in peripheral blood was determined by using ELISA. Myeloid tissue was extracted from all CML cases and ITP patients to detect MVD by using CD34 labelling. At the same time the level of VEGF,b-FGF were detected by using RT-PCR in both peripheral blood and myeloid cells.Results:Our results showed that the concentration of VEGF was obviously higher in the peripheral blood of CML patients(177.53?153.45 pg/ml) than in those of control group(73.12?19.82 pg/ml). The gene expression of VEGF and b-FGF were both higher than those of control group. The difference has statistical significance(P
4.The effects of comprehensive oral health care on caries in orthodonticly treated patients.
Meihong CHEN ; Pu LI ; Shenghui ZHANG
Journal of Practical Stomatology 2000;0(06):-
0.01). The new caries did not occur in the test group. DMF index was higher in the controls than that in the tested subjects(P
5.Enhancing the Core Competitiveness of University Students through Simulative Employment Training Course
Rong PU ; Jingjing LI ; Li QIAN ; Yanlin CHEN ; Chen HAI
Journal of Kunming Medical University 2013;(12):142-144
Objective To explore the validity of simulative employment training course on enhancing the core competitiveness of university students.Method We investigate the students with simulative employment trainin, graduates and recruiters through interview, symposium and questionnaire survey. Results The comprehensive quality and occupational capacity of students that completed simulative employment training had been improved. In the simulative employment training course,98%students thought their interview skills had been promoted, 90%recruiters were satisfied with the students,95%graduates who had worked consider that the simulative employment training were helpful. Conclusions Simulative employment training is effective for enhancing the core competitiveness of university students.
6.Function and structure analysis of premembrane and envelope proteins of Zika virus
Chang LU ; Na LI ; Pu XUE ; Nan LI ; Ping CHEN
Chinese Journal of Zoonoses 2017;33(1):81-84
The premembrane and envelope proteins (prM-E),which contains the mainly protective antigen related with virulence and tropism,are the primary structural protein of flavivirus.However,prM-E in ZIKV is rarely understood.We have analyzed the structure and biological effects of prM-E in ZIKV by bionformatics methods.The prM-E proteins virus-like particles of dengue virus was introduced in the present.Then,the prM-E proteins virus like particles of ZIKV was prospected.
7.Algorithms, Strategies and Application Progress of Spectral Searching Methods
Xiaoli CHU ; Jingyan LI ; Pu CHEN ; Yupeng XU
Chinese Journal of Analytical Chemistry 2014;(9):1379-1386
In recent years, many modern spectral databases for complex materials ( such as soil, feed, forensic evidence materials, pharmaceuticals and oils) have been established based on molecular spectroscopy (UV, infrared, near infrared, Raman and fluorescence) both at home and abroad, which are playing more and more important roles in the agricultural and industrial production and science research. Spectral searching method is one of the key technologies to make full use of the molecular spectral database. This paper reviewed the progress in the basic and modified algorithm, strategy and application of molecular spectral searching methods, and put forward the scientific and technological problems that need attention and further research.
8.Obstructive sleep apnea hypopnea syndrome:risk factors for perioperative
Li PU ; Lizhong SU ; Minyuan CHEN ; Liming PAN ; Shuangfei HU
Chinese Journal of Emergency Medicine 2011;20(2):198-202
Objective To analyze the emergency-event in patients with obstructive sleep apnea-hypopnea syndrome( OSAHS), and to discuss the risk factors and preliminary strategies for prevention of emergency-event. Method A total of 257 OSAHS patients in Zhejiang Provincial Peoples Hospital, were enrolled from January 2000 to December 2009 for the retrospective study. Demographics of patients include age, sex,height, weight, related diseases, AHI and LSaO2 before operation , the way of anesthesia and operation,when and how the emergency-events happened, the way to cure and the results. Risk facts of emergency-event were analyzed by using univariate analysis and multiple logistic regression analysis. Results Of the 257patients, the incidents of emergency-event was 7.3%. The independent risk factors of emergency-event were general anesthesia, multiple level surgery in one time, BMI and AHI. Performing CPAP treatment before operation had a beneficial effect to the avoidance of emergency-event. Conclusions Emergency-event are more common in severe OSAHS patients, especially when they were operated under general anesthesia and multiple level surgery in one time. Performing CPAP treatment before operation had a beneficial effect to the avoidance of emergency-event.
9.Treatment of elderly severe osteoporotic vertebral compressive fractures by kyphoplasty
Hai TANG ; Hao CHEN ; Bingqiang WANG ; Jinjun LI ; Pu JIA
Chinese Journal of Orthopaedics 2010;30(10):978-983
Objective To evaluate the feasibility and therapeutic effect of kyphoplasty in treating severe osteoporotic vertebral compressive fractures. Methods Thirty-five patients (48 vertebral bodies) with severe osteoporotic compressive fractures were included. There were 33 females and 2 males with the mean age of 74.2 years. The average compressive rate of the affected vertebral bodies was 77.0%. The thoracolumbar vertebrae were treated with kyphoplasties. Percutaneous puncture direction was adjusted according to compressive rate and shape of the vertebral bodies. The inflatable bone tamp was inserted into the fractured vertebral body. The balloon was inflated with low pressure and dilate-relieve-dilate method was applied. The balloon was deflated and withdrawn, leaving a cavity within the vertebral body, which then fulfilled with visualized bone cement. Preoperative and postoperative symptom level, complications and radiographic findings were recorded. Results All 35 patients tolerated procedure well. The mean heights of the anterior, mid and posterior vertebral body had improved from (0.8±0.1) cm, (0.8±0.2) cm, (2.1 ±0.8) cm preoperatively to (1.2±0.3) cm, (1.3±0.2) cm, (2.3±1.0) cm respectively after operation (P <0.05). There was significance difference between preoperative and postoperative heights of the anterior and mid vertebral body. The mean kyphosis was improved from 28.2°±5.2° before operation to 19.1°±4.9° after operation. Conclusion Kyphoplasty is feasible and effective for severe osteoporotic vertebral compressive fractures.
10.Study on the mechanism of hyperuricemia among middle and elderly groups
Yuesong LI ; Hongbo PU ; Hongyu WU ; Yongwei CHEN ; Liguo ZHU
Clinical Medicine of China 2010;26(9):959-962
Objective To study the mechanism of the hyperuricemia among the middle and elderly populations. Methods Serum uric acid, creatinine (Cr), blood urea nitrogen (BUN), fasting gluose (FG), total cholesterol (TC), triglyceride (TG) were detected in 1073 subjects with hyperuricemia and 1235 subjects with normal serum uric acid as control of middle and elder groups. Results The means of Cr, BUN, FG, TG ,TC in hyperuricemia were significantly higher than those in the control group,respectively (males: t′ =7. 508,P <0.05;t′ =9. 484,P <0.05;t=6.208,P<0.05;t′ =7.055,P <0.05;t = 5. 097,P <0.05;females;t′ = 11.221,P <0.05;t′= 8.314,P <0.05 ;t =5. 641 ,P <0.05 ;t′ =8. 328 ,P <0.05 ;t =7. 227 ,P < 0.05). In males,the mean of the BUN; FG and TG were significant different among the different age groups (the control group: F = 3. 500, P < 0.05; F = 5. 607, P <0.05 ;F =3. 378,P <0.05 ;the hyperuricemia group: F= 15.400,P <0.05 ;F =5. 111 ,P <0.05 ;F = 11. 143 ,P <0.05), the positive rate of BUN, Cr, FG and TG were significant different among the different age groups (control group:χ2 = 17. 112,P < 0.05;χ2 =7. 807,P <0.05 ;χ2 = 17. 829,P <0.05;χ2=8.433,P <0.05; hyperuricemia group:χ2 =35. 587,P <0.05 ;χ2 =83. 005 ,P <0.05 ;χ2 =41. 639,P <0.05 ;χ2 =31. 466,P <0.05). In the same age group,the mean and the positive rate of BUN and Cr were significantly higher in the hyperuricemia group than in the control group(P < 0.05). The mean of TG was significantly higher in every age group of the hyperuricemia group than controls (P < 0.05), but the positive rate had no significant differences in the age group of ≥ 70 years (P >0.05). The mean and the positive rate of FG and TC were significant differences in middle age group between the hyperuricemia and the control group (P < 0.05), but were no differences in elder age group(P > 0.05). In females,the mean and positive rate of Cr, BUN, FG,TG and TC were significant different in different age groups of the controls(BUN:F = 13. 759,P <0.05;χ2 = 19. 491 ,P <0.05; FG: F = 13. 554,P <0.05;χ2 = 33. 438,P <0.05;TG:F= 18. 160,P <0.05;χ2 = 16. 978,P <0.05;TC: F = 37. 647,P <0.05;χ2 =60.547,P <0.05) ,but in the hyperuricemia group that were only significant difference in BUN, Cr and TC (BUN:F = 5. 830, P < 0.05; χ2 =11.941,P<0.05;Cr:F=4.057,P <0.05;χ2 =20.097,P<0.05;TC:F=7.934,P <0.05;χ2 = 16.405,P <0.05). In same age group compared of all the indices were similar with male. Conclusions The mechanism of serum uric acid increasing are different in middle age and elderly age. In middle age, it is metabolic disturbance. However,in elderly age it is descending of the kidney function.